• The Korean Journal of Mycology
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• v.31 no.2
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• pp.103-113
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• 2003

Twenty five isolates of Alternaria were obtained from various solanaceous crops in Korea. For all isolates, morphological characteristics of the conidia were determined and compared with those of representative isolates of A. solani and A. tomatophila. A selection of the isolates and the representative Alternaria isolates were evaluated for Pathogenicity to potato, tomato, egg plant and red pepper. Molecular characteristics of 17 isolates of Alternaria inculding the representative isolates were determined using sequence analysis of IRS rDNA and histone H3 gene, and URP-PCR analysis. Based on morphological characteristics, the isolates from the solanaceous crops were grouped as identical or very similar to either A. tomatophila (ATO), A. solani (ASO), and unidentified Alternaria sp. (ASP). Isolates of ASO were moderately pathogenic to all the solanaceous crops tested, but ATO isolates were highly pathogenic to tomato and the ASP isolate was pathogenic only to potato. Among the molecular markers used in this study, the URP-PCR analysis was found to be appropriate for taxonomic resolution of these species. Based on the conidial morphology, pathogenicity test and molecular characteristics, A. tomatophila (early blight of tomato) could be distinguished from A. solani (early blight of potato), and the Alternaria sp. (ASP) from potato, which was closely related to ASO in conidial morphology, was considered as a new species.

• Steel and Composite Structures
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• v.39 no.1
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• pp.81-93
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• 2021

Splitting tensile strength (STS) is an important mechanical parameter of concrete. This study offers novel methodologies for the early prediction of this parameter. Artificial neural network (ANN), which is a leading predictive method, is synthesized with two metaheuristic algorithms, namely atom search optimization (ASO) and equilibrium optimizer (EO) to achieve an optimal tuning of the weights and biases. The models are applied to data collected from the published literature. The sensitivity of the ASO and EO to the population size is first investigated, and then, proper configurations of the ASO-NN and EO-NN are compared to the conventional ANN. Evaluating the prediction results revealed the excellent efficiency of EO in optimizing the ANN. Accuracy improvements attained by this algorithm were 13.26 and 11.41% in terms of root mean square error and mean absolute error, respectively. Moreover, it raised the correlation from 0.89958 to 0.92722. This is while the results of the conventional ANN were slightly better than ASO-NN. The EO was also a faster optimizer than ASO. Based on these findings, the combination of the ANN and EO can be an efficient non-destructive tool for predicting the STS.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2361-2366
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• 2013

Diverse studies have shown that miR-155 is overexpressed in different tumor types. However, the precise molecular mechanism of the ectopic expression of miR-155 in breast cancer is still poorly understood. To further explore the role of miR-155 in breast tumorigenesis, we here assessed the influence of miR-155 antisense oligonucleotide (miR-155 ASO) on MDA-MB-157 cell viability and apoptosis in vitro. Furthermore, the effects of inhibitory effects of miR-155 on the growth of xenograft tumors in vivo were determined with performance of immunohistochemistry to detect expression of caspase-3, a pivotal apoptosis regulatory factor, in xenografts. Transfection efficiency detected by laser confocal microscope was higher than 80%. The level of miR-155 expression was significantly decreased (P<0.05) in the cells transfected with miR-155 ASO, compared with that in cells transfected with a negative control. After being transfected with miR-155 ASO, the viability of MDA-MB-157 cells was reduced greatly (P<0.05) and the number of apoptotic cells was increased significantly. Additionally, miR-155 ASO inhibited the growth of transplanted tumor in vivo and significantly increased the expression of caspase-3. Taken together, our study revealed that miR-155 ASO can induce cell apoptosis and inhibit cell proliferation in vitro. Moreover, miR-155 ASO could significantly repress tumor growth in vivo, presumably by inducing apoptosis via caspase-3 up-regulation. These findings provide experimental evidence for using miR-155 as a therapeutic target of breast carcinoma.

• The Journal of Industrial Distribution & Business
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• v.11 no.10
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• pp.75-89
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• 2020

Purpose: The container terminal is an area that plays an important role in the country's import and export. As the volume of containers increased worldwide, competition between terminals became fiercer, and increasing the productivity of terminals became more important. Re-handling is a serious obstacle that lowers the productivity of terminal. There are two ways to reduce re-handling in the terminal yard. The first method is to load containers in terminal yards using effective carry-in algorithms that reduce re-handling. The second method is to carry out effective remarshalling. In this paper, the performance of various carry-in algorithms and various remarshalling algorithms are reviewed. Next, we try to find the most effective combination of carry-in algorithm and remarshalling algorithm. Research design, data and methodology: In this paper, we analyze the performance of the four carry-in algorithms, AP, MDF, LVF, RP and the four remarshalling algorithms, ASI, ASI+, ASO, ASO+. And after making all the combinations of carry-in algorithms and remarshalling algorithms, we compare their performance to find the best combination. To that end, many experiments are conducted with eight types of 100 bays through simulation. Results: The results of experiments showed that AP was effective among the carry-in algorithms and ASO+ was effective among remarshalling algorithms. In the case of the LVF algorithm, the effect of carrying in was bad, but it was found to be effective in finding remarshalling solution. And we could see that ASI+ and ASO+, algorithms that carry out remarshalling even if they fail to find remarshalling solution, are also more effective than ASI and ASO. And among the combinations of carry-in algorithms and remarshalling algorithms, we could see that the combination of AP algorithm and ASO+ algorithm was the most effective combination. Conclusion: We compared the performance of the carry-in algorithms and the remarshalling algorithms and the performance of their combination. Since the performance of the container yard has a significant effect on the performance of the entire container terminal, it is believed that the results of this experiment will be effective in improving the performance of the container terminal when carrying-in or when remarshalling.

• Animal Bioscience
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• v.35 no.1
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• pp.54-63
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• 2022

Objective: This study aimed to evaluate the replacement of degummed soybean oil (DSO) by acid soybean oil (ASO) in diets with or without the inclusion of emulsifier on broiler performance, relative organ weight, lipase activity, intestinal morphometry, and nutrient digestibility. Methods: A total of 704 1-day-old male broiler chicks were allotted to a 2×2 completely randomized factorial design (with or without emulsifier × two lipid sources [ASO and DSO]), with eight replicates and 22 birds each. The metabolizable energy level in diets with emulsifier was reduced by 40 kcal/kg from 1 to 21 d and 50 kcal/kg from 22 to 49 d. Results: Broilers fed diets containing ASO without emulsifier had higher (p = 0.005) weight gain than DSO-fed animals and with the inclusion of emulsifier had worse (p = 0.018) feed conversion ratio (FCR). Birds fed diets with emulsifier worsened FCR regardless of lipid source from 1 to 21 days (p = 0.006) and from 1 to 49 days (p = 0.0002). There was an increase (p = 0.026) in the relative pancreas weight, at 14 days, in birds fed diets containing ASO. Lipase activity and morphometry of the duodenum and jejunum, at 14 and 21 days, were not affected (p>0.05). The dietary inclusion of emulsifier improved the digestible energy (p = 0.053) in the presence of ASO. For the digestibility coefficients (gross energy, crude protein, and mineral matter), no interference was observed (p>0.05). Conclusion: The inclusion of emulsifier to energy-restricted diet with ASO maintained broiler performance in the first week, but worsened FCR in subsequent phases. The ASO can be considered as an alternative lipid source to DSO and does not interfere with the morphophysiological characteristics and performance of broilers. The combination of ASO and emulsifier increased the digestible energy content by 6.2%.

• Journal of Chest Surgery
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• v.20 no.2
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• pp.261-269
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• 1987

From 1976 through 1986, authors have experienced 127 cases of peripheral vascular surgery which had been done in this department. There were 29 cases of atherosclerosis obliterances including 7 Leriche syndrome, 32 Buerger`s diseases, 25 arterial thromboembolisms, 21 vascular injuries, 2 peripheral arterial aneurysms, 2 renovascular hypertensions, 1 congenital A-V malformation, 13 varicose vein of lower extremities, and 2 Jugular venous ectasia. Cases with vena caval disease and aortic disease were excluded. The mean age of ASO and Buerger`s disease was 56.1 yrs, 33.8 yrs respectively. The male to female ratio showed marked male preponderance [27:2, and 30:2], and almost every male patient was smoker. The indication of operation was similar in both disease entities. The method of operation for ASO were bypass procedure [17], thromboendarterectomy [6], and lumbar sympathectomy [5], and for Buerger`s disease were mainly sympathectomy and few bypass procedures and amputations. Seventeen patients with ASO were followed from 3 to 75 month and overall patency rate for bypass or endarterectomy in one and two months and 2 1/2 yr were 93%, 87%, and 31% respectively. Post operatively patient`s symptoms was relieved or alleviated in almost ASO patients, and about 60% of Buerger`s disease. We concluded that in patient with ischemic limb, we must revascularized aggressively for symptomatic relief. And choice of graft for bypass procedure was to be evaluated further.

• Molecules and Cells
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• v.28 no.4
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• pp.341-345
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• 2009

MiRNAs are non-coding RNAs that play a role in the regulation of major processes. The inhibition of miRNAs using antisense oligonucleotides (ASOs) is a unique and effective technique for the characterization and subsequent therapeutic targeting of miRNA function. Recent advances in ASO chemistry have been used to increase both the resistance to nucleases and the target affinity and specificity of these ASOs. Peptide nucleic acids (PNAs) are artificial oligonucleotides constructed on a peptide-like backbone. PNAs have a stronger affinity and greater specificity to DNA or RNA than natural nucleic acids and are resistant to nucleases, which is an essential characteristic for a miRNA inhibitor that will be exposed to serum and cellular nucleases. For increasing cell penetration, PNAs were conjugated with cell penetrating peptides (CPPs) at N-terminal. Among the tested CPPs, Tat-modified peptide-conjugated PNAs have most effective function for miRNA inhibition. PNA-based ASO was more effective miRNA inhibitor than other DNA-based ASOs and did not show cytotoxicity at concentration up to 1,000 nM. The effects of PNA-based ASOs were shown to persist for 9 days. Also, PNA-based ASOs showed considerable stability at storage temperature. These results suggest that PNA-based ASOs are more effective ASOs of miRNA than DNA-based ASOs and PNA-based ASO technology, compared with other technologies used to inhibit miRNA activity can be an effective tool for investigating miRNA functions.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.7
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• pp.1457-1467
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• 2015

Depending on the type of Tx-Rx pairs, VHF Data Exchange System (VDES) for maritime communication is expected to employ the different frequency channels. Load imbalance between the different channels turns out to be a critical problem for the multi-hop communication using Ad-hoc Self-Organizing TDMA (ASO-TDMA) MAC protocol, which has been proposed to provide the connectivity between land station and remote ship stations. In order to handle the inter-channel load imbalance problem, we consider a model of the stochastic geomety in this paper. After analyzing the spatial reuse efficiency in each hop region by the given model, we show that the resource utility can be maximized by balancing the inter-channel traffic load with optimal resource allocation in each hop region.

• Journal of Electrical Engineering and Technology
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• v.9 no.3
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• pp.873-881
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• 2014

Speed sensorless modes of operation are becoming standard solution in the area of electric drives. This paper presents flux estimator and speed estimator for the speed sensorless vector control of induction motors. The proposed sensorless methods are based on the model reference adaptive system (MRAS) observer and adaptive speed observer (ASO). The proposed speed estimation algorithm can be employed in the power control of grid connected induction generator for wind power applications. Two proposed schemes are verified through computer simulation PSIM and compared their simulation results.

• Information and Communications Magazine
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• v.25 no.8
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• pp.55-61
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• 2008

본고에서는 차세대 디지털 지상파 TV표준으로 알려진 DVB-T2(Digital Video Broadcasting-the 2nd Generation Terrestrial)에 대해 소개한다. DVB-T2 기술은 ASO(Analogue Switch Off) 이후의 환경을 위해 DVB프로젝트에 의해 개발되었으며 최신의 변조 및 코딩 기술을 채택하고 있다. 기존 DVB-T에 비해 전송효율을 30% 이상 증가시킬 수 있으며 2010년경에 상용화 될 것으로 예상된다.