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http://dx.doi.org/10.7314/APJCP.2013.14.4.2361

Effects of miR-155 Antisense Oligonucleotide on Breast Carcinoma Cell Line MDA-MB-157 and Implanted Tumors  

Zheng, Shu-Rong (Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College)
Guo, Gui-Long (Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College)
Zhai, Qi (Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College)
Zou, Zhang-Yong (Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College)
Zhang, Wei (Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.4, 2013 , pp. 2361-2366 More about this Journal
Abstract
Diverse studies have shown that miR-155 is overexpressed in different tumor types. However, the precise molecular mechanism of the ectopic expression of miR-155 in breast cancer is still poorly understood. To further explore the role of miR-155 in breast tumorigenesis, we here assessed the influence of miR-155 antisense oligonucleotide (miR-155 ASO) on MDA-MB-157 cell viability and apoptosis in vitro. Furthermore, the effects of inhibitory effects of miR-155 on the growth of xenograft tumors in vivo were determined with performance of immunohistochemistry to detect expression of caspase-3, a pivotal apoptosis regulatory factor, in xenografts. Transfection efficiency detected by laser confocal microscope was higher than 80%. The level of miR-155 expression was significantly decreased (P<0.05) in the cells transfected with miR-155 ASO, compared with that in cells transfected with a negative control. After being transfected with miR-155 ASO, the viability of MDA-MB-157 cells was reduced greatly (P<0.05) and the number of apoptotic cells was increased significantly. Additionally, miR-155 ASO inhibited the growth of transplanted tumor in vivo and significantly increased the expression of caspase-3. Taken together, our study revealed that miR-155 ASO can induce cell apoptosis and inhibit cell proliferation in vitro. Moreover, miR-155 ASO could significantly repress tumor growth in vivo, presumably by inducing apoptosis via caspase-3 up-regulation. These findings provide experimental evidence for using miR-155 as a therapeutic target of breast carcinoma.
Keywords
Breast neoplasms; microRNA-155; antisense oligonucleotide; MDA-MB-157 cells; nude mice;
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