• Applied Biological Chemistry
• /
• v.47 no.1
• /
• pp.135-140
• /
• 2004

The antioxidant activities of 80% methanol extracts obtained from 118 medicinal plants were tested through the evaluation of DPPH and superoxide anion radicals scavenging activity. Methanol extracts of Sophora japonica (76.9%) and Camptotheca acuminata Dence (50.9%) were found to have more than 50% DPPH radical scavenging activity while those of Perilla frutescens (37.2%), Amomum costatum (34.9%), Prunus ansu (33.2%), Mentha arvensis (32.3%), Serratula koreana (32.2%), Eriobotrya japonica (30.5%), and Artemisia asiatica (30.5%) showed more than 30% scavenging activity. Even though all of the commercial antioxidants didn't show superoxide anion radical activity, Mentha arvensis (87.7%), Eriobotrya japonica (84.9%), Amomum costatum (82.9%), Camptotheca acuminata Dence (82.1%) showed more than 80% scavenging activity. Mentha arvensis, Eriobotrya japonica, Amomum costatum, Camptotheca acuminata Dence showed strong antioxidative activity in the both DPPH and superoxide anion radical scavenging activities.

• Molecules and Cells
• /
• v.22 no.1
• /
• pp.104-112
• /
• 2006

We previously reported that DA-9601, ethanol herbal extract of Artemisia asiatica, inhibited histamine and leukotriene releases in guinea pig lung mast cells activated with specific antigen/antibody reaction. This study aimed to evaluate the inhibitory effect of DA-9601 on the OVA-induced airway inflammation in allergic asthma mouse model. BALB/c mice were sensitized and challenged with OVA. DA-9601 was administered orally 1 h before every local OVA-challenge. OVA-specific serum IgE was measured by ELISA, recruitment of inflammatory cells in BAL fluids and lung tissues by Diff-Quik and H&E staining, respectively, the expressions of CD40, CD40L and VCAM-1 by immunohistochemistry, goblet cell hyperplasia by PAS staining, activities of MMPs by gelatin zymography, expressions of mRNA and proteins of cytokines by RT-PCR and ELISA, activities of MAP kinases by western blot, and activity of NF-${\kappa}B$ by EMSA. DA-9601 reduced IgE level, recruitment of inflammatory cells into the BAL fluid and lung tissues, expressions of CD40, CD40L and VCAM-1 molecules, goblet cell hyperplasia, MMPs activity, expressions of mRNA and productions of various cytokines, activities of MAP kinases and NK-${\kappa}B$ increased from OVA-challenged mice. These data suggest that DA-9601 may be developed as a clinical therapeutic agent in allergic diseases due to suppressing the airway allergic inflammation via regulation of various cellular molecules expressed by MAP kinases/NF-${\kappa}B$ pathway.

• The Korean Journal of Physiology and Pharmacology
• /
• v.25 no.5
• /
• pp.439-448
• /
• 2021

DA-9601 is an extract obtained from Artemisia asiatica, which has been reported to have anti-inflammatory effects on gastrointestinal lesions; however, its possible anti-inflammatory effects on the small intestine have not been studied yet. Therefore, in this study, we investigated the protective effects of DA-9601 against the ACF-induced small intestinal inflammation. Inflammation of the small intestine was confirmed by histological studies and the changes in the CD4⁺ T cell fraction induced by the inflammation-related cytokines, and the inflammatory reactions were analyzed. Multifocal discrete small necrotic ulcers with intervening normal mucosa were frequently observed after treatment with ACF. The expression of IL-6, IL-17, and TNF-α genes was increased in the ACF group; however, it was found to have been significantly decreased in the DA-9601 treated group. In addition, DA-9601 significantly decreased the levels of proinflammatory mediators such as IL-1β, GM-CSF, IFN-γ, and TNF-α; the anti-inflammatory cytokine IL-10, on the other hand, was observed to have increased. It is known that inflammatory mediators related to T cell imbalance and dysfunction continuously activate the inflammatory response, causing chronic tissue damage. The fractions of IFN-γ⁺ Th1 cells, IL-4⁺ Th2 cells, IL-9⁺ Th9 cells, IL-17⁺ Th17 cells, and Foxp3⁺ Treg cells were significantly decreased upon DA-9601 treatment. These data suggest that the inflammatory response induced by ACF is reduced by DA-9601 via lowering of the expression of genes encoding the inflammatory cytokines and the concentration of inflammatory mediators. Furthermore, DA-9601 inhibited the acute inflammatory response mediated by T cells, resulting in an improvement in ACF-induced enteritis.

• Journal of Aquaculture
• /
• v.22 no.1
• /
• pp.112-117
• /
• 2009

This study were conducted to investigate the effects of several additives in experimental diets on the growth, feed utilization and body composition of juvenile and grower rockfish. Three replicates of juveniles (3.6 g/fish) and two replicates growers (166 g/fish) were fed the experimental diets containing herb medicines mixture, Artemisia asiatica and Epimedium koreanum for 8 weeks. Dietary supplementation with herb medicines mixture had no beneficial effects on growth and feed utilization of juvenile fish. Weight gain, daily feed intake, condition factor and hepatosomatic index of grower fish fed the diet were not affected by dietary additive (P>0.05). Peed efficiency and protein efficiency ratio of grower fish fed the diet containing herb medicines mixture were significantly higher than those of fish fed the control diet (P<0.05), but not significantly different from fish fed the diet containing A. asiatica and E. koreanum (P>0.05). Proximate analysis of whole body, muscle, viscera and liver in the juvenile and grower were not affected by dietary additives (P>0.05). The results of this study suggest that feed efficiency and protein efficiency ratio of grower rockfish may improved by herb medicines mixture supplementation in the diet.

• Korean Journal of Medicinal Crop Science
• /
• v.13 no.6
• /
• pp.278-286
• /
• 2005

This study was conducted to classify 80 Artemisia spp. collections based on morphological characters and RAPD analysis to get the basic information of Artemisia spp. collections as medicinal plants. The eighty Artemisia spp. collections were classified into 5 groups with the maximum distance 0.82 between clusters based on the complete linkage cluster analysis with morphological traits. Out of 80 operon primer, 10 primers showing polymorphic bands were selected for RAPD analysis. Among the 98 bands amplified with the primers, 68 (69%) bands showed polymorphism. The number of amplified bands ranged from 8 to 10 with an average number of 9.8 bands. Artemisia spp. collections classified into 6 groups with the similarity value of 0.63 in dendrogram derived from the cluster analysis based on RAPDs. Group consisted of 29 collections. Group, which is the largest one, contained 40 collections. Most of the A. asiatica and A. feddei LEV et VNT. were classified into Group and. The rest of the collections (31%) were classified into Group $III{\sim}V$.

• The Korean Journal of Ecology
• /
• v.22 no.1
• /
• pp.59-63
• /
• 1999

To verify allelopathic effects, seed germination and seedling growth test, chemical analysis and bioassay of selected species were carried out with naturally occurring chemicals of Artemisia capillaris. Seed germination ratio of Calamagrostis arundinacea. Youngia denticulata and Lactuca indica var. laciniata showed decrease in proportion to increase in aqueous extracts concentration of A. capillaris. while that of Cosmos bipinnatus and Leonurus sibiricus did not. However, dry weight growth of selected species treated with the same extracts as the above experiment was inhibited remarkably compared to the germination test. In the test at different concentrations of essential oil from A. capillaris, seedling growth of A. princeps var. orientalis and Plantago asiatica was suppressed according to the concentration of the essential oil, and root growth of the selected species was more inhibitory than that of shoot growth. Thirty-six chemical compounds were identified from A. capillaris plant by gas chromatography. Seven compounds out of 36 were bioassayed, and terpinen-4-ol was the most toxic among the tested substances.

• Korean Journal of Medicinal Crop Science
• /
• v.15 no.1
• /
• pp.26-29
• /
• 2007

Extracts of 13 medicinal plants were tested to investigate the improvable effects on microflora growth in the intestines of human. Water extracts of Artemisia capillaris, Taraxacum platycarpum and Ethanol extracts of Allium tuberosum, Artemisia capillaris, Artemisia vulgaris, Plantago asiatica, Schizandra chinensis, Sedum sarmentosum, Taraxacum platycarpum, Zingiber officinale inhibited the growth of C. perfringens. Water and ethanol extracts of Sedum sarmentosum, Schizandra chinensis inhibited the growth of E. coli. While 13 medicinal plants were not remarkably inhibited the growth of B. longum. Therefore, ethanol extracts of Schizandra chinensis, Sedum sarmentosum can be utilized as functional food materials to improve in the intestines.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.23 no.6
• /
• pp.994-1000
• /
• 1994

Hot water extract from mugwort (Artemisia asiatica nakai) leaves and tea inhibited the growth of Bacillus subtilis by adding to the nutrient broth 1.0% and 0.5% concentration, respectively. Among the important compounds that contributing mugwort like flavor to the mugwort leaves and tea were considered, authenic compound of thujone, caryophyllene and farnesol showed bactericidal effect for Escherichia coli, Enterobacter aerogenes, Vibrio paraheaemolyticus, Psudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus when teste by paper disc method ( 8 mm diameter). The mixture of caryophyllene and farnesol was more bactericidal effect for various bacteria than the mixture of thujone, caryophyllene and farnesol was more bactericidal effect for various bacteria that the mixture of thujone, caryophyllene and farnesol or each compounds . Especially, the mixture of caryophyllene and farnesol showed strong bactericidal effect (diameter of inhibition zone>40mm) for Vibrio parahaemolyticus , Enterobacter aerogenes and BAcillus subtilis.

• Proceedings of the Korean Society of Toxicology Conference
• /
• 2001.05a
• /
• pp.146-146
• /
• 2001

Gastroesophageal reflux disease (GERD) is multifactorial in etiology and is characterized by movement of acid and other noxious substances from the stomach into the esophagus. The most severe histologic consequence of chronic gastroesophgeal reflux is Barrett's esophagus, which has been considered as a premalignant condition often leading to the formation of adenocarcinoma of esophagus.(omitted)

• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.129.1-129.1
• /
• 2003

Alcohol, Helicobacter pylori, stress and NSAIDs-activated neutrophils all produce reactive oxygen species (ROS), which play an important role in gastric mucosal damage. Eupatilin is an active component of Artemisia asiatica possessing cytoprotective effect. The effect of eupatilin on the production of ROS and cellular damage in AGS and ECV304 cells were evaluated to prove the cytoprotective action against the above mentioned gastric mucosal cell damages. (omitted)