• Biomolecules & Therapeutics
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• v.8 no.3
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• pp.228-234
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• 2000

We have synthesized a novel platinum(II) coordination complex containing trans-ι-1,2-diaminocy-clohexane (DACH) as a carrier ligand and 1,2-dichloroethane (DCE) as a leaving group. A new series of [Pt(trans-ι-DACH)(DCE)](PC) was evaluated for its cytotoxic activity on MKN-45 human gastric adenocar-cinoma cells and normal primary cultured kidney cells. The new platinum complex has demonstrated high efficacy in the cytotoxicity against MKN-45/P, MKN-45/ADM and MKN-45/CDDP cell-lines. The cytotoxicity of PC against rabbit proximal renal tubular cells, human renal cortical cells and human renal cortical tissues, determined by MTT assay, the [$^3H$]-thymidine uptake arid glucose consumption tests, was found to be quite less than those of cisplatin. Based on these results, this novel platinum(II) coordination complex appears to be better for improving antitumor activities with low nephrotoxicity and is a valuable lead in the development of new, clinically available anticancer chemotherapeutic agents.

• Restorative Dentistry and Endodontics
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• v.16 no.2
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• pp.182-188
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• 1991

The purpose of this study was to clarify the effect of intracana1 medication on the clinical symptoms and the number of visits before canal filling in endodontic treatment The experimental teeth was divided into three groups. The teeth of group 1 was irrigated with saline solution and the teeth of group 2 was irrigated with sodium hypochlorite during biomechanical preparation. The intracanal medicament was not applied in the canals of group 1 and group 2. The teeth of group 3 was irrigated with sodium hypochlorite and hydrogen peroxide during canal enlargement and applied formocresol as intracanal medicament after drying the root canals with paper points. The incidence of interappintment pain and percussion pain after 1 st visit and the number of appointments before canal filling was examined, and the results was statistically compared. The following results were obtained. 1. The incidence of interappointment pain and percussion pain and the number of visits before canal filling was not effected by the types of irrigant and the use of the intracanal medicament 2. There was no significant difference in the incidence of interappintment pain arid percussion pain and the number of appointement before canal filling between vital teeth and non vital teeth, and also between single rooted teeth and multirooted teeth. 3. The use of formocresol as intracanal medicament was not able to aid the elimination of clinical symptoms and to decrease the number of appointment before canal filling.

• Journal of Wetlands Research
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• v.25 no.1
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• pp.26-34
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• 2023

Wetlands are very critical for the conservation of aquatic ecosystems, while also serving as the breeding/ nesting/ resting grounds for water birds. Generally, wetlands support both resident and migratory birds, thus serving as connecting dots in the global flyways. The Rajasthan state of India has two Ramsar sites (Keoladeo National Park and Sambhar Lake) and many other water bodies/wetlands. However, most of these areas are segregated in the eastern, southeastern, southern, and northern parts. In the western part of Rajasthan, where lies the Great Indian or Thar desert, there are no such reported prominent wetlands drawing attention towards a substantial number of resident and migratory water birds. The author is an Indian Forest Service officer, who was posted in the Thar Desert region and during that time had identified a hidden wetland in the desert landscape. This study deliberates on the wetland location and its faunal diversity with prospects of developing the area as a proper wetland conservation zone. India is a signatory to the Central Asian Flyway of migratory species and serves as an important member in terms of having significant wetlands and reported migratory birds count. The need of preserving and bring the arid zone's hidden wetlands to the forefront can serve as an important tool to conserve water birds and comply with worldwide bird migration conservation efforts.

• Journal of Nutrition and Health
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• v.33 no.1
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• pp.23-32
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• 2000

The purpose of this study was to investigate the effcts of n-3, n-6 fatty arid and d-limonene on histopathological and biochemical changes in experimental rat hepatocarcinogenesis. To attain the above objectives, weanling Sprague-Dawley female rats were intraperitoneally injected twice with a dose of diethylnitrosamine(DEN, 50mg/kg body weight) and after 1 week 0.05% phenobarbital was provided with water. Sardine oil rich in n-3 fatty acids and corn oil rich in n-6 fatty acids were fed at 15% by weight and 5% d-limonene was added to the diet in each group. Ten weeks or 20 weeks after DEN treatment, rats were sacrifirced. The formation of glutathione S-transferase placental form positive(GST-P$\^$+/) foci was significantly decreased by the treatment of either sardine oil or d-limonene HMG-CoA reductase activity was not affected by dietary oils and d-limonene. Protein kinase C (PKC) activity was decreased by either sardine oil or d-limonene. Particularly d-limonene decreased the membrane PKC activity. Membrane Cholesterol/Phospholipid(Chol/PL) ratio was significantly decreased by d-limonene in sardine oil group. The data showed that GST-P$\^$+/ foci number was positively correlated with membrane PKC activity and serum cholesterol and negatively correlated with liver cholesterol level. These results suggest informations about the correlation between histopathological and biochemical changes such as cholesterol metabolism and PKC activity in experimental hepatocarcinogenesis and thereby can elucidate the possible mechanism related to the cancer inhibition.(Korean J Nutrition 33(1) : 23-32, 2000)

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.192-192
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• 2017

In this paper we showed the application of European Space Agency's C-band Sentinel-1 Synthetic Aperture Radar (SAR) imagery to identify land subsidence in a heavily groundwater pumping area. We used the repeat pass satellite interferometry method in combination with persistent scattering (PS) interferometric technique to generate and analyze twenty-eight interferograms for the period October 2014 to November 2016. The interferometry results show that land subsidence is more pronounced in the urban areas. Excessive groundwater pumping in the study area is believed to be the main reason for land subsidence. The results are compared with the subsidence rate measured by GPS as reported in other studies and with the mean change in total water storage field of GRACE solutions provided by the Jet Propulsion Laboratory (JPL), the German Research Centre for Geosciences (GFZ) and the Center for Space Research (CSR). The comparison shows persistently decreasing trends during the period of study. A strong reliance of the trend of land subsidence on the temporal decline in total water storage proposes that much of the land subsidence can be attributed to heavy pumping of the groundwater.

• Plant Resources
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• v.7 no.1
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• pp.39-46
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• 2004

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• The Korean Journal of Quaternary Research
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• v.18 no.2 s.23
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• pp.103-104
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• 2004

This paper discuss some results of observed changes of meteorological elements as temperature, precipitation and some extreme indexes in Mongolia. Mongolia is one of the largest landlocked countries in the world. The climate is characterized by a long lasting cold winter, dry and hot summer, low precipitation, high temperature fluctuation and relatively high number of sunny days per year. During last 60 years the annual mean air temperature has risen $1.66^{\circ}C$. Intensive warming of > $2^{\circ}C$ was observed at higher altitudes of high mountains when warming of < $1^{\circ}C$ was observed the Domod steppe and the Gobi Desert. Heat Wave Duration have statistically significant risen trend with increaded number of days by 8-18 at significance level of 95-99.9% depending on geography and Cold Wave Duration have shortened by 13.3 days significance level of 95-99%. In general, by the amount of precipitation, Mongolia falls in semi-arid and arid region. It is 300-350 mm in the high mountain regions while it is only 50-150 mm in Gobi Desert regions. The changes of annual precipitation have very localized character i.e.decreasing at one site and increasing at a sit nearby. Annual precipitation decreased by 30-90 mm in the northern-central region and increased by 2-60 mm in the western and eastern region. The magnitude of alteration changes in precipitation regardless increasing or decreasing is 5-25%. A trends, significant at the level of 90%, found where changes are more than 40 mm or more than 15% of annual mean value. Moreover, the soil moisture resources was decreased in the last 40 years. Specially, moisture contents of the top soil have decreased 2 times(N. Natsagsuren, 2002). Months of June and July in Mongolia is the year that moisture is not inhibiting vegetation growth. Unfortunately, its also found that moisture in this time tends to decrease. Increased temperature, decreased precipitation and soil moisture are most likely resulted in occurences of more intense drought spells that have taken place during the recent years. Intimately, these changes have considerable impact on livestock in Mongolia.

• Animal Bioscience
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• v.34 no.8
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• pp.1415-1424
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• 2021

Objective: Portable laser methane detectors (LMDs) may be an economical means of estimating CH₄ emissions from ruminants. We validated an LMD-based approach and then used that approach to evaluate CH₄ emissions from indigenous dairy cows in a dryland area of Ethiopia. Methods: First, we validated our LMD-based approach in Simmental crossbred beef cattle (n = 2) housed in respiration chambers and fed either a high- or low-concentrate diet. From the results of the validation, we constructed an estimation equation to determine CH₄ emissions from LMD CH₄ concentrations. Next, we used our validated LMD approach to examine CH₄ emissions in Fogera dairy cows grazed for 8 h/d (GG, n = 4), fed indoors on natural-grassland hay (CG1, n = 4), or fed indoors on Napier-grass (Pennisetum purpureum) hay (CG2, n = 4). All the cows were supplemented with concentrate feed. Results: The exhaled CH₄ concentrations measured by LMD were linearly correlated with the CH₄ emissions determined by infrared-absorption-based gas analyzer (r² = 0.55). The estimation equation used to determine CH₄ emissions (y, mg/min) from LMD CH₄ concentrations (x, ppm m) was y = 0.4259x+38.61. Daily CH₄ emissions of Fogera cows estimated by using the equation did not differ among the three groups; however, a numerically greater milk yield was obtained from the CG2 cows than from the GG cows, suggesting that Napier-grass hay might be better than natural-grassland hay for indoor feeding. The CG1 cows had higher CH₄ emissions per feed intake than the other groups, without significant increases in milk yield and body-weight gain, suggesting that natural-grassland hay cannot be recommended for indoor-fed cows. Conclusion: These findings demonstrate the potential of using LMDs to valuate feeding regimens rapidly and economically for dairy cows in areas under financial constraint, while taking CH₄ emissions into consideration.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.3 no.3
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• pp.154-164
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• 2022

Understanding temporal variations in wildlife populations is a prerequisite for conservation planning of wide-ranging species such as tigers (Panthera tigris). We determined the temporal variation in abundance, population growth, and sex ratios at different age and sex stages for a tiger population in Ranthambhore Tiger Reserve, India from November 2007 to February 2011 using motion-sensing cameras. We identified 19 male and 21 female tigers from stripe patterns during 16,110 trap nights within an area covering 233 km². The annual abundance of the population varied from 34.9 (mean)±3.8 (SE) to 23.9±1.5, with a declining trend in the mean annual change of abundance (-12%). The density of adult females remained stable across the study duration, but the densities of adult males and non-breeding tigers fluctuated. The sex ratio was female-biased (0.58 males/female) for breeding tigers and male-biased (1.74 males/female) for non-breeding tigers. Our results reinforce the importance of long-term studies for monitoring the naturally occurring processes in populations to develop population indicators and identify reliable baseline information for conservation and management planning of populations.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.660-666
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• 1997

Changes of chemical and nutritional composition were investigated in low salt-dried Yellow corvenia muscle during frozen storage at $-20^{\circ}C$. Moisture contort of Yellow corvenia was not significantly changed until after 5 months frozen storage. Salinity was $0.28\%$ in fresh Yellow corvenia and $2.30\%$ in the salted sample. Acidity of the fish muscle was increased in excess of 5 months storage. TBA value was increased by a little amount untel 1 month of storage and significantly incraeased in 3 months of storage. Free fatty acid was increased during frozen storage with rapid increase up to 1 month storage. VBN was increased after 1month storage and amino type nitrogen was increased in 5 months of storage. Major free amino acids were glutamic acid, alanine, glycine and lysine, and composition ratio of most free amino acids were not changed untel after 5 months storage. Polyenoic acid contents were decreased after storage and major fatty acids were palmitic acid (16 : 0), stearic arid (18 : 0) and major fatty acids were palmitic acid (16 : 0), stearic acid (18 : 0) and docosahexaenoic acid (22 : 6). From the results of chemical experiments, low salt-dried yellow corvenia would not be stored at $20^{\circ}C$ for more than 5 months.