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Gamma-aminobutyric Acid Content in Commercial Green Tea (시판 녹차의 GABA 함량)

  • Han, Young-Sook
    • Korean journal of food and cookery science
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    • v.23 no.3 s.99
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    • pp.409-412
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    • 2007
  • The aim of this study was to examine the content of bioactive component, ${\gamma}$-aminobutyric acid (GABA) in 7 commercial green teas. The teas were grown in different regions in Sourhtern Korea, and were picked during the middle of the season. The green tea extracts were each derivatized with 6-aminoquinolyl-N-hydroxy-succinimidyl carbamate(AccQ${\cdot}$Fluor), and GABA was detected by a fluorescence detector at arf excitation of 250 nm and emission of 395 nm. The GABA contents varied depending on the regions where the green tea samples were produced. There was no significant difference in GABA contents between samples A and C, where it ranged from from 45.21${\pm}$0.99 (nmol/g) to 63.83${\pm}$0.61 (nmol/g): however, a significantly different amount of GABA was found in the green tea produced in B. The highest GABA content was 210.67${\pm}$0.85 (nmol/g), whereas the lowest content was 3.88${\pm}$0.71 (nmol/g). This variation in GABA contents was probably due to the regional differences, even within the same location, as well as the processing technology, which may retain or develop more GABA components in the final green tea.

Clinical Study of Gamdutang Complex Formula on Patients of Acute Renal Failure due to Paraquat Intoxication

  • Kim Dong Woung
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.3
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    • pp.588-593
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    • 2002
  • Paraquat, one of the potent herbicides, causes fatal damage to many vital organs, when orally ingested, resulting in circulatory failure, respiratory distress syndrome, and a few other serious problems, but there is no known specific antidote against it. Of the possible problems related to paraquat intoxication, oliguric acute renal failure, which has been known to develop within 24 or 48 hours after intoxication, are notoriously life-threatening. So we attempted to investigate the clinical characteristics and progress of paraquat-induced acute renal failure and the therapeutic possibilities of herbal medicines. All of the fifteen subjects were treated with intravenous fluid injection of 5% dextrose saline or 10% dextrose water in conjunction with herbal medicines which were used for oral administration or gargling. Gamdutang, a decoction of Semen Glycin(黑豆 200g) and Radix Glycyrrhizae(甘草 100g) with addition of other herbs when necessary, was administered orally. At the same time, gargling fluid, consisted of Chinese ink(墨汁), char-frying powder of Rhei Rhizoma(大黃炒炭末), Succus phyllostachyos(竹瀝), was used to detoxify the oral cavity. Serum levels of Blood Urea Nitrogen(BUN) and Creatinine reached its peak on the third day of hospitalization, but then decreased and fell within the normal range on the 7th day and remained there. Serum levels of Na+ and K+ decreased down below the lower limits of normal range on the 7th day and on the 3rd day, respectively. Then they returned back within normal limits. Mean urine output on the 1st day of hospitalization was 1,050ml and it continuously increased to reach more than 2,000ml on the 14th day. From that day on, it stayed over 2,000ml. Fifteen cases of acute renal failure caused by paraquat intoxication were treated with combined treatments of oriental and western medicine in our hospital. However, we think that it is necessary to study further about the way to combine oriental and western medicine, to find out a more effective treatment method.

Effects of Moxibustion on Zusanli(SP6), Yinlingquan(SP9) and Pangguangshu(BL28) at Experimental Acute renal failure in rats. (삼음교(三陰交), 음릉천(陰陵泉), 방광유(膀胱兪) 단독(單獨) 및 배혈(配穴) 애구(艾灸) 시술(施術)이 Uranyl Acetate에 의하여 유발(誘發)된 백서(白鼠)의 급성신부전(急性腎不全)에 미치는 영향(影響))

  • Youn Dae-Hwan;Park Jong-Seung;Kim Young-Sung;Kim Jeong-Sang;Na Chang-Su
    • Korean Journal of Acupuncture
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    • v.20 no.2
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    • pp.55-66
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    • 2003
  • Objectives : The aim of this experiment is to investigate the effects of moxibustion on SP6, Sp9 and BL28 in uranyl acetate-induced acute renal failure in rats. Methods : To induce experimental acute renal failure, uranyl acetate was intraperitoneally injected to rats by 5mg/kg. 3 days later moxibustion treatment was done at SP6, Sp9 and BL28 bilaterally of the rats. And then 4 days later, we measured rats' body weight, kidney weight, serum BUN, creatinine and serum electrolytic levels(Na, K and Cl). Results : In the SP6 group, serum BUN were significantly decreased (p<0.01). In the BL28 group, serum Na level were significantly decreased (p<0.05). In the $SP6{\cdot}SP9$ group, serum creatinine level were significantly increased but serum Na level, serum Cl level were significantly decreased(p<0.05). In the $SP6{\cdot}BL28$ group, hypertrophy index of kidney and serum creatinine level were significantly decreased(p<0.01) and serum BUN were marginally significantly decreased (0.1

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Characterization of Phosphatidylcholine-Hydrolyzing Phospholipase D in the Scuticociliate Parasite, Uronema marinum

  • Seo, Jung-Soo;Kim, Moo-Sang;Kim, Na-Young;Ahn, Sang-Jung;Jee, Bo-Young;Jung, Sung-Hee;Kim, Jin-Woo;Kim, Ki-Hong;Lee, Hyung-Ho;Chung, Joon-Ki
    • Journal of fish pathology
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    • v.21 no.1
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    • pp.1-11
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    • 2008
  • We report the existence of new type of phosphatidylcholine-hydrolyzing phospholipase D (PLD), which has been characterized and partially purified in the scuticociliate, Uronema marinum. The enzyme from partial purification showed that it was existed in membrane fraction and was a neutral PLD, which catalyzed both transphosphatidylation and hydrolysis reaction. The activity of partially purified membrane-bound PLD was also found to be optimal at pH 7.0-7.5 for 2 hours at 37℃ and depended strictly on the presence of Ca2+ (2.5 mM) and Mg2+ (1.6 mM). Immunoblot analysis indicated that the enzyme was distinct from hPLD1 (human PLD1) and hPLD2 (human PLD2) because it was not recognized by a polyclonal antibody raised to the 12 terminal amino acid of these enzymes. We also found that the membrane-bound PLD is a PIP2-dependent PLD and that GTP-binding proteins are not implicated in the regulation of this enzyme: This enzyme activity is markedly stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) but not by the small G-protein Arf and GTPrS. In addition, this enzyme was capable of hydrolyzing phosphatidylcholine (PC) but not phosphatidylethanolamine (PE), implying that PC was a preferred substrate.

A Study on Cybersecurity Policy in the Context of International Security (국제협력을 통한 사이버안보 강화방안 연구)

  • Kim, So Jeong;Park, Sangdon
    • Convergence Security Journal
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    • v.13 no.6
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    • pp.51-59
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    • 2013
  • Cyberspace, based on the dramatic development of information and communications technology, has brought enormous benefits to mankind. However, concerns over cyber terrorism and cyber attack are becoming serious. It is time to expand the global dialogue on international security issues in cyberspace. It is imperative to have a common understanding that cyberspace, the infrastructure for prosperity, should not be utilized as a space to create conflicts among states, and that all states agree to build confidence and peace in cyberspace. For this purpose, there are 3 tracks of international cooperations: 1)international cooperation such as UN and Conference on Cyberspace, 2)regional cooperations such as ARF and OSCE. 3)bilateral cooperations such US-Russia Cybersecurity Agreement, US-China presidential level dialogue. This paper will analyze the 1st track of international cooperations of UN and Conference on Cyberspace. With this, Korean government can prepare the forthcoming GGE activities and make our own strategy to deal with the global norms of good behaviour in cyberspace.

Overexpression of GmAKR1, a Stress-Induced Aldo/keto Reductase from Soybean, Retards Nodule Development

  • Hur, Yoon-Sun;Shin, Ki-Hye;Kim, Sunghan;Nam, Kyoung Hee;Lee, Myeong-Sok;Chun, Jong-Yoon;Cheon, Choong-Ill
    • Molecules and Cells
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    • v.27 no.2
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    • pp.217-223
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    • 2009
  • Development of symbiotic root nodules in legumes involves the induction and repression of numerous genes in conjunction with changes in the level of phytohormones. We have isolated several genes that exhibit differential expression patterns during the development of soybean nodules. One of such genes, which were repressed in mature nodules, was identified as a putative aldo/keto reductase and thus named Glycine max aldo/keto reductase 1 (GmAKR1). GmAKR1 appears to be a close relative of a yeast aldo/keto reductase YakC whose in vivo substrate has not been identified yet. The expression of GmAKR1 in soybean showed a root-specific expression pattern and inducibility by a synthetic auxin analogue 2,4-D, which appeared to be corroborated by presence of the root-specific element and the stress-response element in the promoter region. In addition, constitutive overexpression of GmAKR1 in transgenic soybean hairy roots inhibited nodule development, which suggests that it plays a negative role in the regulation of nodule development. One of the Arabidopsis orthologues of GmAKR1 is the ARF-GAP domain 2 protein, which is a potential negative regulator of vesicle trafficking; therefore GmAKR1 may have a similar function in the roots and nodules of legume plants.

Link Adaptation with SNR Offset for Wireless LAN Systems (무선 LAN 시스템에서의 SNR 오프셋을 이용한 링크 적응화)

  • Kim, Chan-Hong;Jeong, Kyo-Won;Ko, Kyeong-Jun;Lee, Jung-Woo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.10A
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    • pp.839-846
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    • 2011
  • Link Adaptation should select the best modulation and coding scheme (MCS) which gives the highest throughput as channel conditions vary. Several link adaptation algorithms for wireless local area network (WLAN) have been proposed but for the future WLAN systems such as 802.11n system, these algorithms do not guarantee the best performance. In this paper, we propose a new link adaptation algorithm in which an MCS level is chosen by the received SNR plus the offset value obtained from the transmission results. The performance of proposed algorithm is simulated by an IEEE 802.11n system. From the analysis, we conclude the proposed algorithm performs better than the well-known link adaptation algorithms such as auto rate fallback and general SNR-based techniques. Particularly, the proposed algorithm improves throughput when the packet error ratio (PER) is constrained for fast fading channels.

Design Flood Estimation using Historical Rainfall Events and Storage Function Model in Large River Basins (과거강우사상과 저류함수모형을 이용한 대유역 계획홍수량 추정)

  • Youn, Jong-Woo;Lee, Dong-Ryul;Ahn, Won-Sik;Rim, Hae-Wook
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.3B
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    • pp.269-279
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    • 2009
  • The design flood estimation in a large river basin has a lot of uncertainties in areal reduction factors, time-spatial rainfall distribution, and parameters of rainfall-runoff model. The use of historical concurrent rainfall events for estimating design flood would reduce the uncertainties. This study presents a procedure for estimating design floods using historical rainfall events and storage function model. The design rainfall and time-spatial distribution were determined through analyzing concurrent rainfall events, and the design floods were estimated using storage function model with a non-linear hydrology response. To evaluate the applicability of the procedure of this study, the estimated floods were compared to results of frequency analysis of flood data. Both floods gave very similar results. It shows the applicability of the procedure presented in this study for estimating design floods in practices.

Clinical Utility of the Minnesota Multiphasic Personality Inventory-Adolescent Restructured Form in the Assessment of Internalizing and Externalizing Disorders in Adolescents: A Preliminary Approach

  • Hye Ji Yun;Eun Hee Park;Hyun Ju Hong
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.34 no.4
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    • pp.268-274
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    • 2023
  • Objectives: This study investigated whether the Minnesota Multiphasic Personality Inventory-Adolescent Restructured Form (MMPI-ARF) can differentiate between two groups of adolescents, one diagnosed with internalizing disorders and another with externalizing disorders, and examined the clinical utility of the MMPI-A-RF by examining which subscales can significantly discriminate between these two groups. Methods: A total of 105 adolescents aged 13-18 years completed the MMPI-A-RF (53 internalizing disorder and 52 externalizing disorder groups). Independent t-test, chi-square test (χ2), and discriminant analysis were used to examine whether MMPI-A-RF can distinguish between the two groups. Results: Sixteen MMPI-A-RF scales best predicted differences between the groups with internalizing and externalizing disorders. Fourteen scales (Higher-Order Scale [Emotional/Internalizing Dysfunction], Restructured Clinical [RC] Scale [RC demoralization, Somatic Complaints (RC1), and Low Positive Emotions (RC2)], Personality Psychopathology Five Scale [Introversion/Low Positive Emotionality-Revised, Negative Emotionality/Neuroticism-Revised], Somatic/Cognitive Scale [Malaise, Head Pain Complaints, and Gastrointestinal Complaints], Internalizing Scale [Stress/Worry, Self-Doubt], Externalizing Scale [Negative School Attitudes], Interpersonal Scale [Social Avoidance, Shyness]) were associated with the internalizing disorder group, whereas two scales (Externalizing Scale [Conduct Problems, Negative Peer Influence]) were associated with the externalizing disorder group. Conclusion: The MMPI-A-RF can be an efficient assessment tool for a quick diagnosis as it can classify individuals with internalizing and externalizing disorders in clinical settings that lack a variety of assessment tools for children and adolescents.

Intercomparison of Shortwave Radiative Transfer Models for Aerosol-laden Atmospheres (에어러솔 대기에서 단파 영역에서의 복사전달모델들의 상호비교)

  • Yoo, Jung-Moon;Jeong, Myeong-Jae;Lee, Kyu-Tae;Kim, Jhoon;Rhee, Ju-Eun;Hur, Young-Min;Kim, Bo-Mi;Lee, Yun-Gon;Lee, Jae-Hwa;Yoon, Jong-Min;Lee, Won-Hak
    • Journal of the Korean earth science society
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    • v.29 no.2
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    • pp.128-139
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    • 2008
  • Intercomparison among the three radiative transfer models (RTMs) which have been used in the studies for COMS, was carried out on the condition of aerosol-laden atmospheres. Also the role of aerosols in the atmospheric radiation budget was analyzed. The results (hereafter referred to as H15) from Halthore et al.'s study (2005) were used as a benchmark to examine the models. Aerosol Radiative Forcing (ARF) values from the three RTMs, calculated under two conditions of Aerosol Optical Thickness (AOT=0.08, 0.24), were systematically underestimated in comparison to H15 in the following shortwave components; 1) direct and diffuse irradiance at the surface, 2) diffuse upward fluxes at the surface and the top of the atmosphere, and 3) atmospheric absorbance. The ARF values for the direct and diffuse fluxes at the surface was $-10{\sim}-40Wm^{-2}$. The diffuse upward values became larger with increasing both AOT and Solar Zenith Angle (SZA). Diffuse upward/downward fluxes at the surface were more sensitive to the SZA than to the atmospheric type. The diffuse downward values increased with increasing AOT and decreasing SZA. The larger AOT led to surface cooling by exceeding the reduction of direct irradiance over the enhancement of diffuse one at the surface. The extinction of direct solar irradiance was due mainly to water vapor in tropical atmospheres, and to both ozone and water vapor in subarctic atmospheres. The effect of water vapor in the tropics was $3{\sim}4$ times larger than that of the ozone. The absorbance values from the three RTMs agree with those from H15 within ${\pm}10%$.