• 한국초전도ㆍ저온공학회논문지
• /
• 제20권1호
• /
• pp.1-10
• /
• 2018

The improvement of critical current properties of $REBa_2Cu_3O_{7-x}$ (REBCO) coated conductors by introducing artificial pinning centers (APCs) is examined with respect to the field-angle anisotropy, high-field performance and relaxation property with time. Nano-rods along the c-axis introduced by PLD method and isotropic nano-particles introduced by TFA-MOD method are treated. The theoretical analysis is also shown to understand the effect of APCs quantitatively. The effects of superconducting layer thickness that influences the high-field performance and relaxation property are also discussed. It is shown that the upper critical field, which is another important factor to determine the high-field property, can be improved by introduction of APCs through electron scattering at interfaces with the superconducting matrix. The optimum critical current property can be obtained by properly designing the morphology and number density of APCs and the superconducting layer thickness.

• IMMUNE NETWORK
• /
• 제11권4호
• /
• pp.196-202
• /
• 2011

Background: B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and dendritic cells, and stimulates B cell proliferation, differentiation, survival, and Ig production. In the present study, we explored the effect of activin A on BAFF expression by APCs. Methods: To investigate the effect of activin A on BAFF expression by mouse APCs, we measured the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA. Results: Activin A markedly enhanced BAFF expression in mouse macrophages and dendritic cells at both the transcriptional and protein levels. SB431542, an activin receptor-like kinase 4 (ALK4) inhibitor, completely abrogated activin A-induced BAFF transcription. Furthermore, overexpression of DN-Smad3 abolished activin-induced BAFF expression at the transcriptional and protein levels. Conclusion: These results demonstrate that activin A can enhance BAFF expression through ALK4-Smad3 pathway.

• Safety and Health at Work
• /
• 제12권4호
• /
• pp.424-431
• /
• 2021

Background: This study aims to introduce the formulation of the regulation for the selection of respirators for accident preparedness chemicals (APCs) according to chemical workplace situations and to determine on-site applicability. Methods: Workplaces were grouped into seven work categories, and APCs were classified into six groups to select adequate respirators. A survey was conducted to enhance the understanding of work situations and adequate respirators. The total number of subjects surveyed in 2018 was 201 managers and handlers, and that in 2019 was 91 handlers and 204 managers. Results: Adequate respirators were allocated to each cell using the matrix method. The study observed an overall lack of understanding of work situations, especially in the operation of open devices, which was the highest at 32.7%. Despite its implementation in 2015, 17.6% and 25.0% of the managers and APCs handlers, respectively, were unaware of the regulations for selecting respirators. Only 70.4% of the APCs handler wore respirators in compliance with regulations. Conclusion: The method for selecting respirators according to work situations using the matrix method is considered reasonable. Thus, this study suggests that the development of educational contents and reinforcing education should be essential steps to increasing awareness of regulations.

• 정보관리학회지
• /
• 제31권3호
• /
• pp.249-270
• /
• 2014

전체 학술지 논문 가운데 오픈액세스(Open Access, OA)가 차지하는 비중은 현재 20%-30%에 이르고 있고, 최근에는 출판 즉시 오픈액세스가 가능한 골드 OA에 대한 관심이 높다. 이 연구는 골드 OA를 출판하기 위한 선결 조건인 '논문 처리 수수료(Article Processing Charges, APC)' 지원에 관한 것으로 연구후원기관과 저자의 재직대학에서 어떠한 정책을 수립하여 실천하고 있는지를 알아보았다. 영국을 비롯한 유럽에서는 수년 동안 연구후원기관의 APC 부담에 관한 타당성 검토와 시범사업을 진행하여 왔고, 현재 본격적으로 시행하고 있는 단계이다. 미국을 비롯한 북미에서는 주요 대학도서관을 중심으로 OA 펀드를 조성하여 APC를 지원하고 있다. 반면에 한국은 연구후원기관과 대학에서 논문의 그린 OA를 추진할 뿐, APC를 지원하여 골드 OA를 출판하는 단계에 이르지 못하고 있다. 주제 분야에 따라 다르지만 국제 학술지에 논문을 출판하는 국내 연구자도 적지 않아 이들의 논문이 골드 OA로 출판된다면 국제적인 파급력은 크게 증진될 전망이다. 이에 국내 연구후원기관과 대학도 골드 OA에 관심을 갖고 OA 전문 학술지와 하이브리드 학술지에서 요구하는 APC를 지원하는 방향으로 정책의 기본 틀을 세우려는 노력을 경주해야 할 것으로 보인다.

• 한국건설관리학회:학술대회논문집
• /
• 한국건설관리학회 2004년도 제5회 정기학술발표대회 논문집
• /
• pp.561-564
• /
• 2004

국내외에서는 크랙실링 공법의 이점 및 도로면 유지보수 공사의 위험 요소를 인식하여 90년대 초반부터 크랙실링 자동화 장비 개발을 위한 연구를 진행하여 왔다. 기존 문헌 고찰과 도로면 크랙실링 자동화 장비(Automated Pavement Crack Sealer; APCS)의 실험실 및 현장 실험 결과, 도로면에 존재하는 크랙 네트워크를 자동으로 탐지하고 모델링하는 과정의 속도와 정확성을 향상시키는 것은 개발된 크랙실링 자동화 장비의 실용화를 위해 매우 중요한 요인으로 인식되었다 그러나, CCD 카메라를 통해 습득된 도로면 영상에서 크랙 네트워크를 완전 자동으로 인식하는 기술은 일반적인 영상 인식 분야에서 보다 외부 환경적인 요인으로 인해 낮은 인식률을 가지고 있다 본 연구를 통해 기존에 개발된 APCS 머신비전 알고리즘의 경우 도로면 영상의 환경 요인에 의해 발생된 문제점들을 많이 해결하였으나 실용화 단계에서 요구되는 크랙 인식률에는 도달하지 못하였다. 따라서, 본 연구의 목적은 기존 APCS 머신 비전 알고리즘의 완전 자동화 방식 크랙 탐지 및 모델링 알고리즘의 문제점을 분석하고 신경망 학습 기법을 이용한 크랙 인식 알고리즘을 개발하는 것이다.

• Biomolecules & Therapeutics
• /
• 제21권1호
• /
• pp.35-41
• /
• 2013

Metformin is widely used for T2D therapy but its cellular mechanism of action is undefined. Recent studies on the mechanism of metformin in T2D have demonstrated involvement of the immune system. Current immunotherapies focus on the potential of immunomodulatory strategies for the treatment of T2D. In this study, we examined the effects of metformin on the antigen-presenting function of antigen-presenting cells (APCs). Metformin decreased both MHC class I and class II-restricted presentation of OVA and suppressed the expression of both MHC molecules and co-stimulatory factors such as CD54, CD80, and CD86 in DCs, but did not affect the phagocytic activity toward exogenous OVA. The class II-restricted OVA presentation-regulating activity of metformin was also confirmed using mice that had been injected with metformin followed by soluble OVA. These results provide an understanding of the mechanisms of the T cell response-regulating activity of metformin through the inhibition of MHC-restricted antigen presentation in relation to its actions on APCs.

• 한국공간구조학회지
• /
• 제12권3호
• /
• pp.29-33
• /
• 2012

• IMMUNE NETWORK
• /
• 제10권2호
• /
• pp.46-54
• /
• 2010

Background: Graft-versus-host disease (GVHD) is initiated when alloreactive donor T cells are primed by host APCs to undergo clonal expansion and maturation. Since there is a controversy regarding the role of nonhematopoietic cells in GVHD, we wanted to investigate the influence of MHC disparity on nonhematopoietic cells on the pathogenesis of GVHD in the MHC-haplomismatched C57BL/6 ($H-2^b$) or DBA/2 $(H-2^b){\rightarrow}$unirradiated ($C57BL/6{\times}DBA/2$) $F_1(BDF_1;\;H-2^{b/d})$ murine model of acute GVHD (aGVHD) or chronic GVHD (cGVHD). Methods: We generated ($BDF_1{\rightarrow}C57BL/6$), ($BDF_1{\rightarrow}DBA/2$), and ($BDF1{\rightarrow}BDF_1$) chimeras and examined GVHD-related parameters and donor cell engraftment in those chimeras. Results: Using this experimental system, we found that 1) severe aGVHD across MHC Ag barrier depends on the expression of nonhematopoietically rather than hematopoietically derived alloAgs for maximal GVHD manifestations; 2) host APCs were sufficient to break B cell tolerance to self molecules in cGVHD, whereas host APCs were insufficient to induce autoimmunity in aGVHD; 3) donor cell engraftment was greatly enhanced in the host with MHC-matched nonhematopoietic cells. Conclusion: Taken together, our results provide an insight into how MHC disparity on GVHD target organs contribute to the pathogenesis of GVHD.

• IMMUNE NETWORK
• /
• 제7권2호
• /
• pp.80-86
• /
• 2007

Background: CD40-activated B (CD40-B) cells might be an attractive source of autologous antigen-presenting cells (APCs) for immunotherapy due to the convenience to obtain from peripheral blood and expand in vitro. Moreover, CD40-B cells were found to be comparable with DCs in their capacity to raise antigen-specific CD8+ T cells. Here, we have established K562 cells expressing CD40L to expand CD40-activated B cells used for APCs. Methods: After activation of B cell by K562/CD40L, CD40-B cells were examined by counting B cell numbers. Surface expression of CD54, CD80, CD86 and HLA class II was measured by flow cytometry. The CD40-B cells were tested for its function as APC by mixed lymphocyte reactions (MLR) and by induction of T cell responses specific for pp65 peptide in vitro. Results: The expansion of B cells by K562/CD40L increased about 6-folds compared with anti-CD40 or K562. Furthermore, the expression of CD54, CD80, CD86 and HLA class II was up-regulated by K562/CD40L. B cells by K562/CD40L showed comparable antigen presentation activity with mature DCs as shown in MLR, INF-${\gamma}$ ELISPOT assay. Conclusion: These results suggest that K562/CD40L could be used to generate activated B cells as potent APCs which could be useful for cellular vaccination and adoptive immunotherapy.

• Journal of Microbiology
• /
• 제39권4호
• /
• pp.300-304
• /
• 2001

Epstein-Barr Virus(EBV)-transformed lymphoblastoid B cell lines, BLCL which expresse antigens, are potential antigen-presenting cells(APCs) for the induction of CTL in vitro. However transfection of BLCLs with subsequent selection by antibiotics is notoriously difficult because plating efficiencies of BLCLsare reported to be 1% or less. To generated stable transfectants of BLCLs we produced high titers of retroviruess encoding pp 65 antigen of human cytomegalovirus of foreign antigens and trans-duced them of BLCLs. The pp 65 gene was cloned into the retroviral vector pLXSN. The recombinant retroviral vector was transfected to ecotropic packaging cell line, CP&E86, and this polyclonal recom-binant retrovirus was transduced to PA317 that is amphotropic pakaging cell line. The titers of colned PA317 amphotropic retroviruses ranged from 5 to $\times$10$^{6}$ colony forming units (CFU)per ml (CFU/ml) We performed three rounds of consecutive transductions to BLCLs in order to improve the clon-ing effieiencies. The expression of recombinant HCMV-pp65 antigen was more than 20% after the final transduction. THe third-transduced BLCLs were easily selected in optimal concentration of G418. BLCLs expressing foreign antigens could be used as target cells for CTL assay and/or as APCs for induction of in vitro CTL responses specific for viral and tumor antigens.