• 한국미생물·생명공학회지
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• 제8권1호
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• pp.33-39
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• 1980

6-APA의 생산에 사용할 Penicillin acylase를 생산하는 야생균주를 분리하여 생리화학적특성을 조사하여 Escherichia속을 동정하였다. Penicillin acylase 생산성이 가장 높은 균주를 선별하여 배양조건을 조사하고 균체를 생산하여 효소를 추출하여 효소의 반응특성을 조사한 결과는 다음과 같다. 1. 분리한 121주의 생리화학적 특성에 의해 47주가 Escherichia 속으로 동정되고 이들중 12주가 penicillin acylase 활성을 나타냈다. 2. 12주중 가장 강력한 P. acylase 활성을 나타낸 균주가 Escherichia No.11 이였다. 3. 이 균주의 효소생산조건은 탄소원은 0.3%-phenylacetic acid, 질소원은 1%-peptone과 1%-yeast extract, amino 산으로는 0.3%, L-glutamic acid가 영향을 주었다. 그리고 배지의 pH는 7.6~8.0, 배양온도는 34~38$^{\circ}C$, 배양시간은 18시간이 최적 생산조건 이였다. 4. 효소는 균체를 초음파에 의해 추출하여 원심 분리하여 상등액을 $0^{\circ}C$에서 보존하여 효소원으로 하였다. 5. 추출효소의 반응특성은 최적 반응온도는 38$^{\circ}C$, PH는 8.0이였고, 1% penicillin G를 기질로 37$^{\circ}C$에서 반응시켰을 때 4시간의 반응에서 이론식의 56%가 6-APA로 변화되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.847-853
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• 2014

There is a lot of debate on the relationship between vitamin D receptor polymorphisms and risk of breast cancer. Herein, we quantitatively analyzed the published case-control studies on this relationship by meta-analysis, performing a bibliographic search from Pubmed and CNKI up to July 31, 2013. The included case-control studies for Fok1, Bsm1, Taq1, Apa1, Cdx2 and Poly-A were 16, 19, 20, 10, 4, 6, respectively. Crude and adjusted odd ratios and 95% confidence intervals were calculated to present and compare the strength of any associations. The results of combined analyses indicated that Fok1, Bsm1, Apa1, Cdx2 and Poly-A were not significantly associated with the risk of breast cancer. In contrast, the tt genotype of Taq1 was a modest risk factor for breast cancer development (tt vs. TT: OR = 1.21, 95% CI: 1.01-1.44). To further confirm the above results, adjusted effects for the six polymorphisms were pooled based on adjusted ORs reported in the original studies. Adjusted ORs of Fok1, Apa1, Cdx2 and Poly-A were similar to the crude ORs. However, Bsm1 and Taq1 showed inconsistent results. For Bsm1, OR for BB vs. bb was 0.85, 95% CI: 0.74-0.98; for Taq1, OR for tt vs. TT was 1.03, 95% CI: 0.92-1.15, and not associated with risk. Subgroup analyses for crude ORs showed some association between Bsm1, Taq1 and breast cancer in Caucasians only, but for adjusted ORs, no associations were found. This meta-analysis suggests that the roles that Fok1, Apa1, Cdx2 and Poly-A polymorphisms play in breast cancer risk are negligible, with Bsm1 and Taq1 as possible exceptions. To be conservative, we still assumed that they may play a modest role in determining breast cancer risk. Further studies are needed to validate our findings.

• 한국환경보건학회지
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• 제30권1호
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• pp.15-21
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• 2004

In an attempt to analyze the characteristics of domestic pathogenic strains of S. pneumoniae, the basic epidemiological charactristics of pathogenic strains such as their serotypes and frequency of penicillin resistance, and pattern of chromosomal DNA from PFGE(pulsed-field gel electrophoresis) were observed. For this study,56 strains of S. pneumoniae isolated from inpatients and outpatients in the four domestic university hospitals were collected from January to December in 1998. Among those strains, a total of 56 pathogenic strains from blood(39 isolates), cerebrospinal fluid(8 isolates) and other specimen(9 isolates) were selected and isolated. The penicillin resistance frequency of those 56 strains was identified with disk diffusion method with 66.1％. From the invasive strains, predominant serotypes were isolated in the order of 19F(12.5％), 23F(10.7%), 14(10.7%) and 9V(10.7％), totalling 45 percent. This experiment also used PFGE patterns to compare the correlations among genetic subtypes in several serotypes. The DNA fragments digested with Sma I and Apa I were resolved by PFGE. The PFGE patterns digested with Sma I were better than Apa I for analysis. In the DNA fragments digested with Sma 1, PFGE analysis of 56 S. pneumoniae isolates showed 25 different patterns. As a result, serotype was on the whole correlated to PFGE pattern on the ground that each different PFGE pattern by serotype was observed. This study can be utilized not only fur the study of incidence trend of domestic pneumococcal diseases but also as a useful basic data for the development of identification tool and treatment.

• Journal of Microbiology and Biotechnology
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• 제8권2호
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• pp.146-151
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• 1998

The aminoglycoside multiple-resistance determinant from Streptoalloteichus hindustanus was cloned into Streptomyces lividans and named nbrB. The 1.2-kb ApaI- BclI fragment encompassing nbrB was located within a 2.6-kb ApaI fragment by successive subcloning experiments. The complete DNA nucleotide sequence of 1.2-kb containing nbrB was determined. The sequence contains an open reading frame that putatively encodes a polypeptide of 281 amino acids with a predicted molecular weight of 30,992. The deduced amino acid sequence of nbrB shows identities of 85.1% to kgmB of S. tenebrarius, 59.6% to sgm of Micromonospora zionensis, and 57.7% to grm of M. rosea. The similarity of nbrB to kgmB suggests that nbrB encodes a 16S rRNA methylase similar to that encoded by kgmB and that both genes might be derived from a common ancestral gene.

• The Journal of Korean Physical Therapy
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• 제29권3호
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• pp.135-141
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• 2017

Purpose: This study was to identify the anticipatory postural adjustment (APA) mechanism which is represented by the onset time of trunk muscles and the displacement of the center of pressure (COP) according to the different base of support (BOS) during upper extremity movement. Methods: Thirty healthy subjects (14 males, 16 females) participated in this study. The movement was performed for 10 trials during each of various BOS (shoulder - width double leg stance, narrow base double leg stance, tandem stance, non-dominant single leg stance) at the 1.2 Hz frequency. Electromyography was used to measure muscle onset time and biorescue was used to measure characteristics of the displacement of COP. Surface bipolar electrodes were applied over the right deltoid anterior, right latissimus dorsi, both rectus abdominis, both internal oblique and both erector spinae. The data were analyzed by repeated one-way ANOVA and Duncan's post hoc test. Results: The study has revealed following. There were significant differences with muscle onset time in each BOS (p<0.01). There were significant differences in characteristics of the COP in each BOS (p<0.01). Conclusion: The study found that the more narrowed the basis requires the more rapid anticipatory postural control in contralateral postural muscle when the upper extremity movement is performed.

• 한국도로학회논문집
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• 제20권4호
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• pp.7-13
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• 2018

PURPOSES : In order to apply high-speed weigh-in-motion (HS WIM) systems to asphalt pavement, three high-durability asphalt concrete mixtures installed with a WIM epoxy are evaluated. METHODS : In this study, dynamic stability, number of loading repetitions to reach the rut depth of 1 mm, and rut depth measurements of three asphalt mixtures at $60^{\circ}C$ were compared using an Asphalt Pavement Analyzer (APA). Laboratory-fabricated material and field core samples were prepared and tested according to KS F2374. RESULTS : Through the laboratory tests, it was found that all three modified asphalt mixtures (stone-mastic, porous, and semi-rigid) with WIM epoxy showed favorable permanent deformation results and passed the dynamic stability criterion of 3000 loading repetitions per 1 mm. In addition, it was confirmed that the modified SMA mixtures cored from the field construction yields satisfactory rutting testing results using the APA. Finally, the epoxy used for the HS WIM installation shows good adhesion with the three asphalt mixtures and permanent deformation resistance.

• 한국미생물·생명공학회지
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• 제9권4호
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• pp.197-205
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• 1981

Bacillus megaterium의 발효액으로 부터 페니실린 아미다제를 셀라이트에 흡착시켜 분리한 후 이 흡착효소를 아크릴아마이드에 포괄시켜 고정화하였다. 관형식 반응조에서의 이 고정화효소의 안정도는 포괄시키지 않은 흡착효소에 비해 크게 증가하였으며 최적 반응 pH는 8.7, 그리고 최적 안정도는 7.5~8.0이었고 최적온도는 5$0^{\circ}C$ 였다. Km과 6-APA, 페닐초산에 의한 저해상수는 각각 4.55mM, 36.5mM, 그리고 10.5mM이었다. Effectiveness factor값은 0.95로 내부확산 효과는 무시할 수 있었다. pH8.0의 조건에서 관형식 반응조 내에서의 효소역가의 반감기는 4$0^{\circ}C$에서 6.8일 그리고 3$0^{\circ}C$ 에서는 47일로 포괄하지 않은 흡착효소에 비해 안정도가 각각 6.8배와 12배로 증가하였다. 이 고정화 효소에 의한 회분식 및 연녹식반응조에서의 6-APA의 생산성을 논의하였다. 실험결과로 미루어 보아 특히 흡착효소를 고정화효소로 사용하는 경우에 포괄방법을 이용함으로써 효소반응조의 안정도를 크게 증가시킬 수 있음을 시사하였다.

• Korean Chemical Engineering Research
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• 제48권1호
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• pp.45-52
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• 2010

본 연구에서는 Dual-Ovenable Packaging 용 폴리에테르이미드의 수분흡수 특성을 개선하기 위하여 가교구조를 형성할 수 있는 말단기를 포함한 이미드 올리고머를 신규 합성하였으며, 이를 이용한 폴리에테르이미드와 가교구조 (semi-IPN, semi-interpenetrating network)를 형성을 통하여 수분흡수와 열적 특성 변화, 그리고 구조적 특성에 대하여 조사하였다. 가교 말단기와 용액 이미드화법을 이용하여 두 종류의 이미드 올리고머 6FDA-ODA/APA와 6FDA-MDA/MA를 제조하였으며, 그 특성을 고찰하였다. 또한 이미드 올리고머와 $Ultem^{(R)}$ 혼합물의 semi-IPN 구조를 제조하였으며, 열적 성질, 수분흡수 거동, 그리고 박막의 구조적 특징을 TGA, Thin Film Diffusion Analyzer, 그리고 XRD 분석을 이용하여 해석하였다. 제조한 이미드 올리고머는 말단기의 양을 이용하여 분자량 조절이 가능하였다. 그리고, 온도가 증가함에 따라 가교에 의한 발열반응이 일어나며, 가교 가능한 말단기가 증가할수록 발열량이 증가하였다. $Ultem^{(R)}$과 이미드 올리고머 혼합물에 의한 semi-IPN 구조는 이미드 올리고머의 비율이 증가할수록 5와 10% 질량감소 온도가 상승하였다. 또한, 수분에 대한 친화도가 작은 불소기를 함유하는 이미드 올리고머와 패킹정도의 증가로 인하여 semi-IPN의 확산계수와 수분흡수량이 감소하였다. 이와 같이 열적 성질과 수분에 대한 안정성이 떨어지는 단점을 semi-IPN 구조를 통하여 보완할 수 있었다.

• 한국전문물리치료학회지
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• 제24권2호
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• pp.9-18
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• 2017

Background: Limitation of hamstring extensibility is often associated with various musculoskeletal problems such as alterations in posture and walking patterns. Thus, certain appropriate strategies need to be established for its management. Objects: The aim of this study was to compare the effects of the neural mobilization technique and static stretching exercises on popliteal angle and hamstring compliance in young women with short hamstring syndrome (SHS). Methods: Thirty-three women with SHS were randomly assigned to either group-1 ($n_1=17$) that underwent the neural mobilization technique or group-2 ($n_2=16$) that underwent the static stretching exercises. Outcome measures included the active popliteal angle (APA) and a hamstring's electromyographic (EMG) activity at a maximum popliteal angle of the baseline. Intervention for each group was performed for a total time of 3-min (6 sets of a 30-sec application). Results: There were significant interactions between time and group in the APA [group-1 (pre-test to post-test): $69.70{\pm}8.14^{\circ}$ to $74.14{\pm}8.07^{\circ}$ and group-2: $68.66{\pm}7.42^{\circ}$ to $70.52{\pm}7.92^{\circ}$] (F1,31=6.678, p=.015) and the EMG activity of the hamstring (group-1: $1.12{\pm}.30{\mu}N$ to $.69{\pm}.31{\mu}V$ and group-2: $1.19{\pm}.49{\mu}V$ to $1.13{\pm}.47{\mu}V$)(F1,31=6.678, p=.015). Between-group comparison revealed that the EMG activity of the hamstring was significantly different at post-test between the groups (p<.05). Furthermore, in within-group comparison, group-1 appeared to be significantly different for both variables between pre- and post-test (p<.05); however, group-2 showed significant difference in only the APA between pre- and post-test (p<.05). Conclusion: These findings suggest that the neural mobilization technique and static stretching exercises may be advantageous to improve hamstring compliance in young women with SHS, resulting in a more favorable outcome in the neural mobilization technique.

• 약학회지
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• 제30권5호
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• pp.232-237
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• 1986

A series of modified poly(acrylic acid)'s containing different levels of 6-aminopenicillanic acid (6-APA), 7-aminocephalosporanic acid (7-ACA) and 6-[D-(-)-$\alpha$-aminophenyl acetamido] penicillanic acid (ampicillin) as pendant groups were prepared. Antibiotic activities of the newly prepared drugs were examined against the various gram-positive and gram-negative bacteria. It was found that ampicillin modified composition posses antibiotic activities against the gram-negative as well as the gram-positive bacteria.