Journal of Microbiology and Biotechnology
- Volume 8 Issue 2
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- Pages.146-151
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Cloning and Sequence Analysis of the Aminoglycoside Resistance Gene from a Nebramycin Complex Producer, Streptoalloteichus hindustanus
- Hyun, Chang-Gu (Department of Biological Science, Myong Ji University) ;
- Kim, Jong-Woo (Department of Biotechnology, Central Lab. of Research, Dong-Wha Pharmaceutical Co. Ltd.) ;
- Han, Jae-Jin (Department of Biotechnology, Central Lab. of Research, Dong-Wha Pharmaceutical Co. Ltd.) ;
- Choi, Young-Nae (Department of Biotechnology, Central Lab. of Research, Dong-Wha Pharmaceutical Co. Ltd.) ;
- Suh, Joo-Won (Department of Biological Science, Myong Ji University)
- Published : 1998.04.01
Abstract
The aminoglycoside multiple-resistance determinant from Streptoalloteichus hindustanus was cloned into Streptomyces lividans and named nbrB. The 1.2-kb ApaI- BclI fragment encompassing nbrB was located within a 2.6-kb ApaI fragment by successive subcloning experiments. The complete DNA nucleotide sequence of 1.2-kb containing nbrB was determined. The sequence contains an open reading frame that putatively encodes a polypeptide of 281 amino acids with a predicted molecular weight of 30,992. The deduced amino acid sequence of nbrB shows identities of 85.1% to kgmB of S. tenebrarius, 59.6% to sgm of Micromonospora zionensis, and 57.7% to grm of M. rosea. The similarity of nbrB to kgmB suggests that nbrB encodes a 16S rRNA methylase similar to that encoded by kgmB and that both genes might be derived from a common ancestral gene.
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