• The Korean Journal of Physiology and Pharmacology
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• 제21권3호
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• pp.293-300
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• 2017

Prostaglandin $D_2$ ($PGD_2$) may act against myocardial ischemia-reperfusion (I/R) injury and play an anti-inflammatory role in the heart. Although the effect of $PGD_2$ in regulation of ANP secretion of the atrium was reported, the mechanisms involved are not clearly identified. The aim of the present study was to investigate whether $PGD_2$ can regulate ANP secretion in the isolated perfused beating rat atrium, and its underlying mechanisms. $PGD_2$ (0.1 to $10{\mu}M$) significantly increased atrial ANP secretion concomitantly with positive inotropy in a dose-dependent manner. Effects of $PGD_2$ on atrial ANP secretion and mechanical dynamics were abolished by AH-6809 ($1.0{\mu}M$) and AL-8810 ($1.0{\mu}M$), $PGD_2$ and prostaglandin $F2{\alpha}$ ($PGF2{\alpha}$) receptor antagonists, respectively. Moreover, $PGD_2$ clearly upregulated atrial peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and the $PGD_2$ metabolite 15-deoxy-${\Delta}12$, 14-$PGJ_2$ (15d-$PGJ_2$, $0.1{\mu}M$) dramatically increased atrial ANP secretion. Increased ANP secretions induced by $PGD_2$ and 15d-$PGJ_2$ were completely blocked by the $PPAR{\gamma}$ antagonist GW9662 ($0.1{\mu}M$). PD98059 ($10.0{\mu}M$) and LY294002 ($1.0{\mu}M$), antagonists of mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) and phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signaling, respectively, significantly attenuated the increase of atrial ANP secretion by $PGD_2$. These results indicated that $PGD_2$ stimulated atrial ANP secretion and promoted positive inotropy by activating $PPAR{\gamma}$ in beating rat atria. MAPK/ERK and PI3K/Akt signaling pathways were each partially involved in regulating $PGD_2$-induced atrial ANP secretion.

• Animal cells and systems
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• 제6권3호
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• pp.253-261
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• 2002

Although cardiac distribution of specific receptors for atrial natriuretic peptide (ANP) was mainly observed in the ventricular endocardium, the modulation of ANP receptors in relation to cardiac development is not defined. The present study was undertaken to investigate ANP receptor modulation in rat during development. In the developmental stages examined (fetus, after postnatal 3-days, 1-, 2-, 3-, 4-, and 8-week-old Sprague Dawley rats) specific ANP binding sites were localized in the right and left ventricular endo-cardia by quantitative in vitro receptor autoradiography using (equation omitted)-rat ANP as labeled ligand. The specific bindings to endocardium were much higher in the right than the left ventricle. The binding affinities of ANP were much higher in the right than the left ventricular endocardium. The difference of these binding affinities among various developmental stages was not observed in the right ventricle, whereas the binding affinity in left ventricle was gradually increased with aging and reached the peak value at 8 weeks. No significant difference in maximal binding capacities of endocardial bindings was observed in the right and left ventricular endocardia during developmental stages. Also, cGMP production via activation of particulate guanylyl cyclase-coupled receptor subtypes in the ventricular membranes was gradually decreased with close relationship to aging. Therefore, the present study show that the endocardial ANP receptor is modulated with close relationship to cardiac development in the left ventricle rather than the right ventricle, and may be involved in regulating myocardial contractility in left heart.

• 대한약침학회지
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• 제17권3호
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• pp.62-69
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• 2014

Objectives: The purpose of this study is to report a case series of advanced cancer patients whose cancer pain was relieved by using autonomic nerve pharmacopuncture (ANP) treatment. ANP is a subcutaneous injection therapy of mountain ginseng pharmacopuncture (MGP) along the acupoints on the spine (Hua-Tuo-Jia-Ji-Xue; 0.5 cun lateral to the lower border of the spinous processes of vertebrae) to enhance the immune system and to balance autonomic nerve function. Methods: Patients with three different types of cancer (gastric cancer, lung cancer, colon cancer with distant metastases) with cancer pain were treated with ANP. 1 mL of MGP was injected into the bilateral Hua-Tuo-Jia-Ji-Xue on the T1-L5 sites (total 12-20 mL injection) of each patient's dorsum by using the principle of symptom differentiation. During ANP treatment, the visual analogue scale (VAS) for pain was used to assess their levels of cancer pain; also, the dosage and the frequency of analgesic use were measured. Results: The cancer pain levels of all three patients improved with treatment using ANP. The VAS scores of the three patients decreased as the treatment progressed. The dosage and the frequency of analgesics also gradually decreased during the treatment period. Significantly, no related adverse events were found. Conclusion: ANP has shown benefit in controlling cancer pain for the three different types of cancer investigated in this study and in reducing the dosage and the frequency of analgesics. ANP is expected to be beneficial for reducing cancer pain and, thus, to be a promising new treatment for cancer pain.

• 한국산학기술학회논문지
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• 제12권10호
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• pp.4354-4358
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• 2011

많은 국내 건설 회사들은 지속되는 건설 경기 침체로 힘든 시간을 보내고 있다. 따라서 건설기업들이 어려운 상황 속에서 살아남기 위해서는 효율적인 운영과 경쟁력 강화를 위한 경영 효율성의 분석이 필요하다. 본 논문에서는 국내 상장건설기업 효율성을 분석하기 위하여 ANP와 DEA의 결합모델을 제안한다. DAE에서 가장 중요한 투입요소와 산출요소를 결정하기 위하여 ANP 모델을 적용하여 투입요소와 산출요소의 중요도를 평가한다. 그리고 비효율성을 갖는 그룹으로 분류된 기업들에 대해서는 효율성을 갖는 벤치마킹 기업들과 이들 그룹으로 이동하기 위하여 개선해야할 효율성 수치를 제공한다. 본 연구에서는 KOSPI에 상장된 36개와 KOSDAQ에 상장된 21개로 구성된 57개의 건설기업을 분석하였다. 분석결과 CCR 모형에서 효율적으로 선정된 기업은 총 11개였고, BCC 모형에서는 총 14개 기업이 효율성을 가지고 있었다. 추가적으로 규모의 효율성이 1인 기업은 19개 기업이었다.

• 한국콘텐츠학회논문지
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• 제12권11호
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• pp.295-302
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• 2012

디스플레이 산업은 전체 한국 경제에서 매우 중요한 역할을 하고 있다. 디스플레이 기업의 보다 효율적인 운영과 보다 경쟁력 강화를 위한 경영 효율성의 분석이 필요함에도 불구하고, 디스플레이 기업의 효율성을 분석한 경험적 연구는 수행되지 않았다. 본 연구의 목적은 ANP와 DEA 모델을 이용한 상장 디스플레이 기업의 효율성 측정하고 분석하는 것이다. 본 연구에서는 2010년말 KOSPI에 상장된 7개와 KOSDAQ에 상장된 37개로 구성된 44개의 상장 디스플레이 기업을 대상으로 하이브리드 ANP와 DEA 모델을 이용하여 기업의 효율성을 분석하였다. DEA에서 가장 중요한 투입요소와 산출요소를 결정하기 위하여 ANP 모델을 적용하여 투입요소와 산출요소의 중요도를 평가하였다. 그리고 비효율성을 갖는 그룹으로 분류된 기업들에 대해서는 효율성을 갖는 벤치마킹 기업들과 이들 그룹으로 이동하기 위하여 개선해야 할 효율성 수치를 제공하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제15권5호
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• pp.273-277
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• 2011

Nitric oxide (NO) and atrial natriuretic peptide (ANP) may induce vascular relaxation by increasing the production of cyclic guanosine monophosphate (cGMP), an important mediator of vascular tone during sepsis. This study aimed to determine whether regulation of NO and the ANP system is altered in lipopolysaccharide (LPS)-induced kidney injury. LPS (10 $mg{\cdot}kg^{-1}$) was injected in the tail veins of male Sprague-Dawley rats; 12 hours later, the kidneys were removed. Protein expression of NO synthase (NOS) and neutral endopeptidase (NEP) was determined by semiquantitative immuno-blotting. As an index of synthesis of NO, its stable metabolites (nitrite/nitrate, NOx) were measured using colorimetric assays. mRNA expression of the ANP system was determined by real-time polymerase chain reaction. To determine the activity of guanylyl cyclase (GC), the amount of cGMP generated in response to sodium nitroprusside (SNP) and ANP was calculated. Creatinine clearance decreased and fractional excretion of sodium increased in LPS-treated rats compared with the controls. Inducible NOS protein expression increased in LPS-treated rats, while that of endothelial NOS, neuronal NOS, and NEP remained unchanged. Additionally, urinary and plasma NOx levels increased in LPS-treated rats. SNP-stimulated GC activity remained unchanged in the glomerulus and papilla in the LPS-treated rats. mRNA expression of natriuretic peptide receptor (NPR)-C decreased in LPS-treated rats, while that of ANP and NPR-A did not change. ANP-stimulated GC activity reduced in the glomerulus and papilla. In conclusion, enhancement of the NO/cGMP pathway and decrease in ANP clearance were found play a role in the pathogenesis of LPS-induced kidney injury.

• 한국수자원학회논문집
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• 제39권1호
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• pp.35-46
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• 2006

본 연구에서는 안전하고 원활한 용수공급을 목적으로 하는 상수도관망시스템의 오염예방 및 위험관리를 위한 통합의사결정시스템의 기본구조를 제시하고 유럽과 미국에서 널리 사용되고 있는 다기준 의사결정기법인 PROMETHEE와 ANP를 적용해 상수도관망의 이상징후 판정을 위한 위험요소들의 우선순위를 평가하였다. 문제 구성을 위하여 pH 잔류염소농도, 유량, 수압, 전기전도도, 탁도, 블록누수량, 수온을 자료항목으로 선정하였고 관부식, 관파열, 관내수질오염을 평가기준으로 하여 PROMETHEE와 ANP의 결과를 비교 분석하였다. 본 연구에서 제시한 상수도관망의 위험요소 평가결과는 위기상황 대처방안시스템 구축시 사고대응 제어알고리즘 설계의 기초자료가 될 수 있을 것이다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2011년도 학술발표회
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• pp.195-195
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• 2011

국가하천의 주변지역을 체계적이고 계획적으로 조성 이용하여 난개발을 방지하고 지속가능한 발전을 도모하며, 그에 따른 이익을 하천의 정비 및 관리 등에 활용함으로써 공공복리의 증진에 기여함을 목적으로 하는 친수구역활용에 관한 특별법이 곧 시행될 예정이다. 친수는 단순히 하천 보전과 상대되는 수변이용의 측면이 아니라 하천의 경관, 생태계, 물 등 전체적인 하천공간에서 이루어지는 인간의 활동과 심리적 만족감으로 해석할 수 있다. 이러한 친수활동 유형을 정립하기 위하여 전문가 및 실무자들의 도움으로 설문지를 작성하고, 조사하였다. 또한 평가 항목간의 중요도를 합리적으로 부여하고자 전문가 집단 활용을 통한 다기준의사결정 기법 중 계층분석과정 AHP(Analytic Hierarchy Process)와 이의 일반적인 형태인 ANP(Analytic Network Process) 기법을 이용하여 비교 분석하여 보았다. AHP는 의사결정의 목표 또는 평가기준이 다수이며 복합적인 경우, 다수의 속성들을 계층적으로 분류하여 각 속성의 중요도를 파악함으로써 최적 대안을 선정하는 기법으로 다수의 목표, 다수의 평가기준, 다수의 의사결정주체가 포함되어 있는 의사결정 문제를 계층화하여 해결한다. 그러나 의사결정 문제는 대부분 요소들 간의 수평적 상호작용과 수직종속성이 존재하기 때문에 단순히 계층적 구조만으로는 설명할 수 없는 경우가 있다. ANP는 AHP 분석을 확장한 개념으로 평가 요소가 계층구조를 가지고 있으면서 각 평가 요소간 네트워크 구조를 가지는 경우에 해당한다. 즉, 시스템 분석, 통합 및 조정을 위한 방법으로 요소들 간의 비선형 관계를 모델화하는 복잡한 의사결정에 적용이 가능하다. ANP는 AHP의 기본 공리인 독립성이 유지되지 않을 때도 적용할 수 있는 의사결정 방법이기 때문이다. AHP와 ANP 분석을 위해 평가기준은 하천특성, 생태환경특성, 인문환경특성, 경관, 유지관리 등으로 분류하였으며, 세부평가항목은 하천의 물리 생태학적 특성, 개발여건과 관련된 특성, 경관의 이용가치, 유지관리 용이성 등 20여 개로 나누었다. AHP와 ANP를 이용하여 친수공간 이용지수 가중치를 산정한 결과, 두 기법은 큰 차이를 보이지 않았다. 그러나 ANP를 이용하여 가중치를 결정하는 방법은 최종 결정의 공정성이나 신뢰성을 확보하는데 기여를 하는 것으로 나타났다.

• The Korean Journal of Physiology and Pharmacology
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• 제26권6호
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• pp.469-478
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• 2022

WNT signaling plays an important role in cardiac development, but abnormal activity is often associated with cardiac hypertrophy, myocardial infarction, remodeling, and heart failure. The effect of WNT signaling on regulation of atrial natriuretic peptide (ANP) secretion is unclear. Therefore, the purpose of this study was to investigate the effect of Wnt agonist 1 (Wnta1) on ANP secretion and mechanical dynamics in beating rat atria. Wnta1 treatment significantly increased atrial ANP secretion and pulse pressure; these effects were blocked by U73122, an antagonist of phospholipase C. U73122 also abolished the effects of Wnta1-mediated upregulation of protein kinase C (PKC) β and γ expression, and the PKC antagonist Go 6983 eliminated Wnta1-induced secretion of ANP. In addition, Wnta1 upregulated levels of phospho-transforming growth factor-β activated kinase 1 (p-TAK1), TAK1 banding 1 (TAB1) and phospho-activating transcription factor 2 (p-ATF2); these effects were blocked by both U73122 and Go 6983. Wnta1-induced ATF2 was abrogated by inhibition of TAK1. Furthermore, Wnta1 upregulated the expression of T cell factor (TCF) 3, TCF4, and lymphoid enhancer factor 1 (LEF1), and these effects were blocked by U73122 and Go 6983. Tak1 inhibition abolished the Wnta1-induced expression of TCF3, TCF4, and LEF1 and Wnta1-mediated ANP secretion and changes in mechanical dynamics. These results suggest that Wnta1 increased the secretion of ANP and mechanical dynamics in beating rat atria by activation of PKC-TAK1-ATF2-TCF3/LEF1 and TCF4/LEF1 signaling mainly via the WNT/Ca²⁺ pathway. It is also suggested that WNT-ANP signaling is implicated in cardiac physiology and pathophysiology.

• 한국자원식물학회지
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• 제25권6호
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• pp.693-699
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• 2012

This study was conducted to investigate the effects of Woohwangcheungsimweun (ox bezoar), deer antlers, and wild ginseng on induction of cardiomyocyte differentiation using the established mouse embryonic stem (ES) cells. The expression of atrial natriuretic peptide (ANP) was highest in Woohwangcheungsimweun treatment group. The expression of rabbit anti-GATA-4(GATA-4) and troponin (TnI) were highest in wild ginseng and Woohwangcheungsimweun treatment groups, respectively. Fluorescence activated cell sorting (FACS) analysis showed that the expression of ANP was highest in Dimethyl sulfoxide(DMSO) and Woohwangcheungsimweun treatment groups. The expression of GATA-4 was relatively high in wild ginseng treatment group. The expression of TnI was highest in Woohwangcheungsimweun treatment group. In the gene expression analysis, DMSO greatly inhibited GATA-4 expression to 25% of control. Woohwangcheungsimweun treatment caused to increase cTnI and cardiac ANP expression significantly. Wild ginseng extract upregulated GATA-4 gene expression. In conclusion, DMSO widely used as cardiomyocyte differentiation inducer did not show significant effects on the expression of ANP, GATA-4 and TnI in this study. Woohwangcheungsimweun showed upregulation of ANP and TnI expression. Wild ginseng extract showed greater effects than DMSO on GATA-4 expression. These results might suggest that the combination of Woohwangcheungsimweun and wild ginseng extract treatment can be expected to increase expressions of all three genes.