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The role and characterization of .betha.-1, 3-glucanase in biocontrol of fusarium solani by pseudomonas stutzeri YPL-1

  • Lim, Ho-Seong;KiM, Sang-Dal
    • Journal of Microbiology
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    • v.33 no.4
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    • pp.295-301
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    • 1995
  • An antifungal Pseudomonas stutzeri YPL-1 produced extracellular chitinase and .betha.-1, 3-glucanase that were key enzymes in the decomposition of fungal hyphal walls. These lytic extracellular enzymes markedly inhibited mycelial growth of the phytopathogenic fungus Fusarium solani. A chitinase from P. stutzeri YPL-1 inhibited fungal mycelial growth by 87%, whereas a .betha.-1, 3-glucanase from the bacterium inhibited growth by 53%. Furthermore, co-operative action of the enzymes synergistically inhibited 95% of the fungal growth. The lytic enzymes caused absnormal swelling and retreating on the fungal hyphal walls in a dual cultures. Scanning electron microscopy clearly showed hyphal degradation of F. solani in the regions interacting with P. stutzeri YPL-1. In an in vivo pot test, P. stutzeri YPL-1 proved to have biocontrol ability as a powerful agent in controlling plant disease. Planting of kidney bean (Phaseolus vulgaris L.) seedlings with the bacterial suspension in F. solani-infested soil significantly suppressed the development of fusarial root-rot. The characteristics of a crude preparation of .betha.-1, 3-glucanase produced from P. stutzeri YPL-1 were investigated. The bacterium detected after 2 hr of incubation. The enzyme had optimum temperature and pH of 40.deg.C and pH 5.5, respectively. The enzyme was stable in the pH range of 4.5 to 7.0 and at temperatures below 40.deg.C, with a half-life of 40 min at 60.deg.C.

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Occurrence of Web Blight in Soybean Caused by Rhizoctonia sol ani AG-l(IA) in Korea

  • Kim, Wan-Gyu;Hong, Sung-Kee;Han, Seong-Sook
    • The Plant Pathology Journal
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    • v.21 no.4
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    • pp.406-408
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    • 2005
  • Web blight symptoms were frequently observed on soybean plants grown in a farmer's fields located in Jincheon in Korea during a disease survey in August, 2005. Incidence of the disease was $5-20\%$ infected plants in two of four soybean fields investigated. A total of 31 isolates of Rhizoctonia sp. were obtained from leaves, leaf petioles, and pods of diseased soybean plants. The isolates were identified as Rhizoctonia solani AG-l(IA) by anastomosis test and based on the morphological and cultural characteristics. Three isolates of R. solani AG-l(IA) were tested for pathogenicity to five cultivars of soybean by artificial inoculation. All the isolates induced blight symptoms on the leaves of soybean and formed sclerotia on the lesions, which were similar to those observed in the field. The pathogenicity tests revealed that all the soybean cultivars tested were susceptible to the pathogen. There was no difference in the pathogenicity among the isolates. The present study first reveals that R. solani AG-l(IA) causes web blight of soybean in Korea.

Knowledge Sharing Behavior in Indonesia: An Application of Planned Behaviour Theory

  • NEGARA, Danes Jaya;FERDINAND, Ferdinand;MEITIANA, Meitiana;ASTUTI, Maria Haryulin;ANDEN, Trecy;SARLAWA6, Rita;MAHRITA, Ani
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.3
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    • pp.1053-1064
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    • 2021
  • The objective of this research is to empirically study public servant knowledge sharing intention by implementing the Theory of Planned Behavior (TPB) of the Sustainable Development Goals (SDGs). The researcher proposes an extended TPB combined with a perceived environmental concern variable as a determinant of behavioral intention. A total of 150 questionnaires were distributed among civil society and the public sector at the district level in the Central Kalimantan Province of Indonesia. Collectively, 126 inquiries were received. A Partial Least Squares (PLS) analysis was applied to analyze and test the research model and hypothesis. The results show that all of the components of the TPB significantly influenced perceived SDG realization. The findings also partially show that knowledge sharing intention mediated the effect of attitude, perceived behavioral control, and perceived environmental concern on SDG realization. This study confirms that perspectives that affect knowledge sharing intention among public sectors could be approached based on the offered model. Hence, the local government can implement the suggestion offered in this research. The originality of this paper lies in the fact that this study discloses factors affecting knowledge sharing among public servants.

Effect of Intangible Assets on the Value Relevance of Accounting Information: Evidence from Emerging Markets

  • AL-ANI, Mawih Kareem;TAWFIK, Omar Ikbal
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.2
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    • pp.387-399
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    • 2021
  • This study mainly aims to examine the effect of intangible assets on the value relevance of the Gulf Cooperation Council (GCC)-listed non-financial firms. This study tested three types of models by using a large sample of non-financial firms listed in GCC countries as emerging markets from 2008 to 2016. The types of models are accounting information (earnings per share and book value of share) without intangible assets model, intangible assets model, and accounting information (earnings per share and book value of share) with intangible assets model. Ordinary least square (OLS) shows mixed results as intangible assets improve the value relevance of accounting information positively in UAE and negatively in Kuwait but not in other countries. The study documents a robust positive relationship between intangible assets and earnings quality in terms of value relevance in KSA and Qatar. The findings provide implications for policymakers, investors, and managers. The results suggest that intangible assets can improve the value relevance in emerging markets, such as GCC, as the need to organize the requirements of information disclosures on intangible assets and provide great transparency and additional disclosure of information about intangible assets and their components.

Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex

  • Pramunadipta, Syafiqa;Widiastuti, Ani;Wibowo, Arif;Suga, Haruhisa;Priyatmojo, Achmadi
    • The Plant Pathology Journal
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    • v.38 no.3
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    • pp.254-260
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    • 2022
  • Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.

Neopestalotiopsis Leaf Blight, an Emerging Concern on Leatherleaf Fern in Indonesia

  • Ani Widiastuti;Indah Khofifah Aruan;Alvina Clara Giovanni;Barokati Tsaniyah;Tri Joko;Achmadi Priyatmojo
    • Research in Plant Disease
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    • v.30 no.1
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    • pp.82-87
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    • 2024
  • Leatherleaf fern (Rumohra adiantiformis) is an important ornamental plant in Indonesia and global. Green fern leaves with bold dark green color with long shelf-life, attract florists as decoration. Indonesia is one important leatherleaf fern exporters, however currently an outbreak of leaf blight decreased production significantly. Initial symptom was reddish brown spots from edge of leaf, which was gradually followed by dark-brown necrotic lesions causing leaf blight and dried. This is a study to do Koch-Postulate approach and molecular identification, to identify the pathogen of the "new emerging disease" reported. Based on multigene analysis using primers from ITS, β-tub and tef1-α gene markers, the pathogen was identified as Neopestalotiopsis sp. All sequences have been deposited in GenBank with accession number of OR905551 (ITS), OR899817 (ßtubulin) and OR899816 (TEF). This Neopestalotiopsis leaf blight causes an emerging concern in leatherleaf fern in Indonesia and global biosecurity because it infected an export commodity.

A Study on the Visual Evaluation of the Gather Effect in Ruffle (Ruffle의 gather 효과에 대한 시각평가의 연구)

  • Kwon Young-Suk;Moon Meyng-Ok
    • Journal of the Korean Society of Clothing and Textiles
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    • v.11 no.1
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    • pp.43-49
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    • 1987
  • To study the visual evaluation on the effect of ruffle's gather, the aesthetic evaluation on the gather's measurements and the image evaluation were performed. Evaluated fabrics were cotton, wool ani synthetic fiber, and 3 kinds of thickness for each fabric were selected. In order to seek for aesthetic gather's measurements, we performed the sensory test by the Rank Method on 5 kinds of gather's measurements(I.5 times, 1. 8times, 2times, 2.5 times, 3 times). For the image evaluation on the effect of the gather, we performed the sensory test by the Semantic Differential Method on the gather's measurements were got high score in the sensory test of the gather's measurements and analyzed by means of a Factor Analysis. The results were as follows. 1. Except 2 times of fabric $A_1$(thin cotton), the aesthetic gather's measurements of the ruffle were evaluted 2.5 times in cotton and synthetic fiber, and 2 times in wool. Generally the aesthetic gather's measurements of the ruffle were 2$\~$2.5 times. 2. The image characteristics of the ruffle were established the characteristics of the fabrics as factor 1, the characteristics of the atomosphere as factor 2 and the characteristics of the drape as factor 3. Therefore, we must consider the characteristics of the fabric, the atomosphere and the drape of the ruffle in production of the ruffle.

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NONLINEAR VARIATIONAL INEQUALITIES AND FIXED POINT THEOREMS

  • Park, Sehie;Kim, Ilhyung
    • Bulletin of the Korean Mathematical Society
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    • v.26 no.2
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    • pp.139-149
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    • 1989
  • pp.Hartman and G. Stampacchia [6] proved the following theorem in 1966: If f:X.rarw. $R^{n}$ is a continuous map on a compact convex subset X of $R^{n}$ , then there exists $x_{0}$ ..mem.X such that $x_{0}$ , $x_{0}$ -x>.geq.0 for all x.mem.X. This remarkable result has been investigated and generalized by F.E. Browder [1], [2], W. Takahashi [9], S. Park [8] and others. For example, Browder extended this theorem to a map f defined on a compact convex subser X of a topological vector space E into the dual space $E^{*}$; see [2, Theorem 2]. And Takahashi extended Browder's theorem to closed convex sets in topological vector space; see [9, Theorem 3]. In Section 2, we obtain some variational inequalities, especially, generalizations of Browder's and Takahashi's theorems. The generalization of Browder's is an earlier result of the first author [8]. In Section 3, using Theorem 1, we improve and extend some known fixed pint theorems. Theorems 4 and 8 improve Takahashi's results [9, Theorems 5 and 9], respectively. Theorem 4 extends the first author's fixed point theorem [8, Theorem 8] (Theorem 5 in this paper) which is a generalization of Browder [1, Theroem 1]. Theorem 8 extends Theorem 9 which is a generalization of Browder [2, Theorem 3]. Finally, in Section 4, we obtain variational inequalities for multivalued maps by using Theorem 1. We improve Takahashi's results [9, Theorems 21 and 22] which are generalization of Browder [2, Theorem 6] and the Kakutani fixed point theorem [7], respectively.ani fixed point theorem [7], respectively.

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Substantial Study on Constituent Elements of the Foot Taeyang Meridian Muscle in the Human Truncus

  • Park, Kyoung-Sik
    • The Journal of Korean Medicine
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    • v.30 no.3
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    • pp.15-27
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    • 2009
  • Objective : This study was carried to identify the anatomical component of BMM (Foot Taeyang Meridian Muscle in the human truncus), and further to help the accurate application to real acupunctuation. Methods: The human truncus was stripped off in order to demonstrate muscles, nerves and other components, and to display the internal structure of the BMM, dividing into outer, middle, and inner parts. Results: The BMM in the human truncus is composed of muscles, nerves, ligaments etc. The internal composition of the BMM in the human truncus is as follows: 1. Muscle A. Outer layer: medial palpebral ligament, orbicularis oculi, frontalis, galea aponeurotica, occipitalis, trapezius, latissimus dorsi, thoracolumbar fascia, gluteus maximus. B. Middle layer: frontalis, semispinalis capitis, rhomboideus minor, serratus posterior superior, splenius cervicis, rhomboideus major, latissimus dorsi, serratus posterior inferior, levator ani. C. Inner layer: medial rectus, superior oblique, rectus capitis, spinalis, rotatores thoracis, longissimus, longissimus muscle tendon, longissimus muscle tendon, multifidus, rotatores lumbaris, lateral intertransversi, iliolumbaris, posterior sacroiliac ligament, iliocostalis, sacrotuberous ligament, sacrospinous ligament. 2. Nerve A. Outer layer: infratrochlear nerve, supraorbital n., supratrochlear n., temporal branch of facial n., auriculotemporal n., branch of greater occipital n., 3rd occipital n., dorsal ramus of 1st, 2nd, 3rd, 4th, 5th, 6th, 7th, 8th, 9th, 10th, 11th, 12th thoracic n., dorsal ramus of 1st, 2nd, 3rd, 4th, 5th lumbar n., dorsal ramus of 1st, 2nd, 3rd, 4th, 5th sacral n. B. Middle layer: accessory nerve, anicoccygeal n. C. Inner layer: branch of ophthalmic nerve, trochlear n., greater occipital n., coccygeal n., Conclusions : This study shows that BMM is composed of the muscle and the related nerves and there are some differences from already established studies from the viewpoint of constituent elements of BMM at the truncus, and also in aspect of substantial assay method. In human anatomy, there are some conceptional differences between terms (that is, nerves which control muscles of BMM and those which pass near by BMM).

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microRNA-214-mediated UBC9 expression in glioma

  • Zhao, Zhiqiang;Tan, Xiaochao;Zhao, Ani;Zhu, Liyuan;Yin, Bin;Yuan, Jiangang;Qiang, Boqin;Peng, Xiaozhong
    • BMB Reports
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    • v.45 no.11
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    • pp.641-646
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    • 2012
  • It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3' untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. miR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation.