• Title/Summary/Keyword: AMS medium

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EPS Production, PHB Accumulation and Abiotic Stress Endurance of Plant Growth Promoting Methylobacterium Strains Grown in a High Carbon Concentration

  • Woo, Sung-Man;Subramanian, Parthiban;Ramasamy, Krishnamoorthy;Joe, M. Melvin;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.4
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    • pp.572-581
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    • 2012
  • In this study, we compared growth pattern, floc yield, Exo-polysaccharides (EPS) production, Poly-${\beta}$-hydroxybutyrate (PHB) accumulation, resistance to osmotic and acid stress in Methylobacterium strains CBMB20, CBMB27, CBMB35, and CBMB110. Modified high C:N ratio medium denoted as HCN-AMS medium was used with a C:N ratio of 30:1. The HCN-AMS medium favored increased growth in all the studied strains. All Methylobacterium strains tested positive for EPS production and showed positive fluorescence with calcoflour stain. Elevated levels of EPS production from 4.2 to 75.0% was observed in HCN-AMS medium. Accumulation of PHB in HCN-AMS medium increased by 3.8, 36.7, and 12.0% in strains CBMB27, CBMB35, and CBMB110 respectively. Among the abiotic stresses, osmotic stress-induced growth inhibition of Methylobacterium strains was found to be lowered when grown in HCN-AMS medium. Likewise, growth inhibition due to acid stress at pH 5.0 was lower for strains grown in HCN-AMS medium compared to growth in AMS medium. Enhanced survivability under stress conditions may be attributed to the high EPS and PHB production at increased carbon concentration in the growth medium.

A Node-Grouping MAC Protocol in Delay-Tolerant Underwater Acoustic Sensor Networks (지연 허용적인 수중 센서 네트워크에서 노드 그룹핑을 이용한 매체 접속 제어 프로토콜)

  • Cheon, Jin-Yong;Son, Kweon;Jang, Youn-Seon;Cho, Ho-Shin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.10B
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    • pp.1200-1209
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    • 2011
  • In this paper, we propose a novel energy efficient MAC protocol which is based on orthogonal frequency division multiple access (OFDMA) and exploits the physical characteristic that propagation loss of acoustic wave depends on the distance. In the proposed scheme, sensor nodes are grouped according to the distance to sink node. Then, each group uses a different frequency band. The proposed scheme not only enables all sensor nodes to maintain the signal-to-noise ratio above a certain required level (Accepted Minimum SNR, AMS), but also reduces overall transmission power consumption. In addition, the dynamic sub-channel allocation is employed in order to improve data transmission rate. Simulations show that proposed MAC protocol has better performance in a delay-tolerant underwater acoustic sensor networks.

Statistical Life Expectancy Calculation of MV Cables and Application Methods (중전압 전선의 통계적 수명예측 계산과 응용 방법)

  • Chong-Eun, Cho;On-You, Lee;Sang-Bong, Kim;Kang-Sik, Kim
    • KEPCO Journal on Electric Power and Energy
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    • v.8 no.2
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    • pp.61-68
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    • 2022
  • In this paper, the change history of various types of MV (Medium Voltage) cables was investigated. In addition, the statistical life expectancy of each type was calculated by using the operation data and the failure data. For cut-off year, 10 years was applied, and realistically applicable statistical life expectancy was calculated by correcting the cause of failure entered by mistake. The life expectancy of FR-CNCO-W was calculated as 51.2 years, CNCV-W 38.1 years, and CNCV 31.4 years and the overall average is 33.8 years. Currently, the life expectancy of TR CNCV-W is 29.4 years, but it is estimated that the lifespan will be extended if failure data is accumulated. As a result, it is expected that life expectancy results can be applied to Asset Management System (AMS) in the future.

Optimization of gibberellic acid production by Methylobacterium oryzae CBMB20 (지베렐린산 생산을 위한 Methylobacterium oryzae CBMB20의 최적 배양조건 확립)

  • Siddikee, Md. Ashaduzzaman;Hamayun, Muhammad;Han, Gwang-Hyun;Sa, Tong-min
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.4
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    • pp.522-527
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    • 2010
  • Gibberellic acid ($CA_3$) is used in many industries and constitutes the primary gibberellins produced by fungi and bacteria. However, there is no information on $CA_3$ production by Methylobacterium oryzae CBMB20, a novel plant growth promoting bacterium. We investigated the favorable carbon (C) and nitrogen (N) sources and ratios and cultural conditions, such as incubation temperature, pH of the culture medium, and incubation period for the maximum production of $CA_3$ by Methylobacterium oryzae CBMB20. Maximum $CA_3$ production was observed in ammonium mineral salt (AMS) broth supplemented with Na-succinate and $NH_4Cl$ as C and N sources, respectively. The maximum $CA_3$ production was found at the C/N ratio of 5:0.4 g $L^{-1}$. The highest $CA_3$ production was obtained when the bacterial culture was incubated at $30^{\circ}C$ for 96 h at pH 7.