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Optimization of gibberellic acid production by Methylobacterium oryzae CBMB20  

Siddikee, Md. Ashaduzzaman (Dept. of Agricultural Chemistry, Chungbuk National University)
Hamayun, Muhammad (Dept. of Botany, Abdul Wali Khan University)
Han, Gwang-Hyun (Dept. of Agricultural Chemistry, Chungbuk National University)
Sa, Tong-min (Dept. of Agricultural Chemistry, Chungbuk National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.43, no.4, 2010 , pp. 522-527 More about this Journal
Abstract
Gibberellic acid ($CA_3$) is used in many industries and constitutes the primary gibberellins produced by fungi and bacteria. However, there is no information on $CA_3$ production by Methylobacterium oryzae CBMB20, a novel plant growth promoting bacterium. We investigated the favorable carbon (C) and nitrogen (N) sources and ratios and cultural conditions, such as incubation temperature, pH of the culture medium, and incubation period for the maximum production of $CA_3$ by Methylobacterium oryzae CBMB20. Maximum $CA_3$ production was observed in ammonium mineral salt (AMS) broth supplemented with Na-succinate and $NH_4Cl$ as C and N sources, respectively. The maximum $CA_3$ production was found at the C/N ratio of 5:0.4 g $L^{-1}$. The highest $CA_3$ production was obtained when the bacterial culture was incubated at $30^{\circ}C$ for 96 h at pH 7.
Keywords
Methylobacterium oryzae; Gibberellic acid; Cultural parameter; Na-succinate; Ammonium chloride;
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