• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.5 no.2
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• pp.55-59
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• 2024

The remarkable economic growth achieved in the modern era has revitalized various industries, including pet trade. More than 2,000 species of non-native species have been introduced to South Korea and approximately 7.7 tonnes of alien turtles are imported annually. Turtles belonging to the family Kinosternidae. They are mostly small and popular pets, although they are designated as Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) species. In the present study, we present the first two species of musk turtles found in the wild in South Korea. On April 5, 2023 and July 8, 2023, an eastern musk turtle (Sternotherus odoratus) and a razorbacked musk turtle (Sternotherus carinatus) were captured in Ilsandong-gu, Goyang-si, Gyeonggi-do and Jung-gu, Daejeon, respectively. The carapace length was 88.6 mm for S. odoratus and 105.68 mm for S. carinatus. They were identified based on their morphological characteristics and mtDNA cytochrome b gene. These turtles were found in waterfront areas with over 1.4 million annual visitors. Both sites were found to have threatened amphibians and reptiles. There is an urgent need to continue monitoring and conducting risk assessments for the protection of endemic species in Korea.

• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1412-1419
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• 2023

Retroviral integration into ancient vertebrate genomes left traces that can shed light on the early history of viruses. In this study, we explored the early evolution of retroviruses by isolating nine Spuma endogenous retroviruses (ERVs) and one Epsilon ERV from the genomes of Agnatha and Chondrichthyes. Phylogenetic analysis of protein sequences revealed a striking pattern of co-evolution between jawless fish ERV and their host, while shark ERV underwent ancient cross-class viral transmission with jawless fish, ray-finned fish, and amphibians. Nucleotide sequence analysis showed that jawless fish ERV emerged in the Palaeozoic period, relatively later than ray-finned fish ERV. Moreover, codon analysis suggested that the jawless fish ERV employed an infection strategy that mimics the host codon. The genealogical diversity of ERVs in the jawless fish genome highlights the importance of studying different viral species. Overall, our findings provide valuable insights into the evolution of retroviruses and their interactions with their hosts.

• Journal of Photoscience
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• v.9 no.2
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• pp.267-268
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• 2002

A vertebrate retina is an organ belonging to the central nerve system (CNS), and is usually difficult to regenerate except at an embryonic stage in life. However, certain species of urodele amphibians, such as newts and salamanders, possess the ability to regenerate a functional retina from retinal pigment epithelial (RPE) cells even as adults. After surgical removal of neural retinas from adult newt eyes, the remaining RPE cells lose their pigment granules, transdifferentiate into retinal progenitor cells, which further differentiate into various retinal neurons, and then finally reform a functional neural network. To understand the molecular mechanisms of CNS regeneration, we attempted to investigate the genes expressing in regenerating newt retina. mRNAs were isolated from regenerating retinas at 18-19 days after the surgical removal of the normal retina, and a cDNA library (regenerating retinal cDNA library) were constructed. Our EST analysis of 112 clones in the regenerating cDNA library revealed that about 70% clones are closely related to the genes previously identified. About 40% clones are housekeeping genes, and about 15% clones encode proteins related to the regulation of gene expression and to the proliferation of the cells. Sequences similar to neural retina- and RPE-specific genes were not detected at all. These results led us to suppose that the regenerating retinal cells are in a state considerably different from those of neither neural retina nor RPE cells.

• Animal cells and systems
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• v.5 no.1
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• pp.51-57
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• 2001

We have previously shown that oocyte maturation is induced by an immobilized progesterone, progesterone-3-carboxymethyloxime - bovine serum albumin conjugate (P-BSA) in Rana dybowskii. In this study, we confirmed the maturation inducing activity of P-BSA on Xenopus oocyte and examined the binding character of the immobilized progesterone on the surface of Xenopus oocytes after removal of the vitelline layer. P-BSA induced maturation of Xenopus oocytes but E-BSA failed to do so as observed in Rana. Binding of the immobilized progesterone, fluorescein isothiocyanate-labeled progesterone-3-0-carboxymethyloxime-BSA (P-BSA-FITC) on the devitellined oocytes surface was examined by fluorescence confocal microscopy. The binding affinity of P-BSA-FITC to the devitellined oocyte was higher than that of estrogen-BSA-FITC (E-BSA-FITC) or testosterone-BSA-FITC (T-BSA-FITC). The binding disappeared in the presence of excess free progesterone but not in the presence of free estrogen. Maximum binding occurred after two-hours of incubation with P-BSA-FITC at pH 7.5. Stronger binding occurred in oocytes at stage Vl than stage IV, and in vitro treatment of hCG enhanced the binding. Taken together, these results suggest that a specific receptor for progesterone exists on the plasma membrane of Xenopus oocytes and that progesterone acts initially on this putative receptors and triggers generation of membrane-mediated second messengers during the early stage of oocyte maturation In amphibians.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.5
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• pp.65-77
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• 2014

The ecosystem of Jaemin stream, flowing into the center of Gongju-si, had been damaged by low water quality and lack of water quantity of the steam. However, after applying the SSB (Sustainable Structured wetland Biotop) system to the flood plain and the upstream of Jaemin stream, the efficiency of ecological water purification and ecological restoration are as follows. Through the constant maintenance and monitoring from year 2009 to year 2013 after restorative design and construction the average influent concentration of BOD5 was 4.2 mg/L, and the average effluent concentration was 1.8 mg/L, reaching ecological water purification rate of 57%. As for the T-N, the average influent concentration was 9.983 mg/L, and the average effluent concentration was 6.303 mg/L, showing the rate of 37%. For the T-P, the average influent concentration was 0.198 mg/L, and the average effluent concentration was 0.098 mg/L, being the rate of 51%. The vegetation of Jaemin stream monitored for 2 years after the restoration was composed of 51 species in 28 families which show high ratio of planted native species. As for the animals in the site, 5 species in 3 families of reptiles and amphibians, 34 species of 23 families of birds, and 3 species in 2 families of mammals were monitored, indicating that the bio-diversity of the site has improved, as well.

• Journal of Environmental Science International
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• v.21 no.8
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• pp.997-1007
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• 2012

This study was performed to assess functions of 10 man-made wetlands in Gunsan City, Jeollabuk Province by means of RAM (Rapid Assessment Method), a technique recommended by Ramsar Convention to appraise wetlands. The assessment of the wetlands value found Gunsan reservoir to have the highest function points while Bukchosan reservoir was estimated to have the lowest function among the wetlands surveyed. By detailed factors of valued 10 man-made wetland, the wetlands were found to function most favorably in terms of vegetational variety and as habitat for wild animals. They need to have more improved functions as habitats for fish and amphibians reptiles, for preservation and betterment of water quality as well as for supplement of underground water, though. From the assessment of vegetational naturalness of the surveyed reservoirs, Gunsan reservoir turned out to be the most favorable wetland from the viewpoint of vegetation science whereas, however, Anjeong wetland was rated as the lowest in the assessment of vegetational value due to simplicity in its vegetation and lots of dangers in its neighborhood threatening its vegetation and ecology. The assessment of vegetational values for Gunsan, Gongchang, Daewi, Bukchosan, Anjeong, Geumgul, Changan and Chuksan reservoirs showed the same orextremely similar results as RAM function assessment. Geumsan reservoir only, however, featured the opposite result.

• Journal of Forest and Environmental Science
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• v.35 no.4
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• pp.281-284
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• 2019

Efficient management of road-kill data is difficult at national scale when there are many organizations that are in charge of different road types. Here, we described the first case to integrate road-kill data through Korea Road-kill Observation System (KROS) by the Korean government. The system was launched in June 2018 to approximately 3,000 road menders. During 15 months, 5,812 road-kill observations were registered on KROS including mammals, birds, reptiles and amphibians. Of them, about 86 % was occupied by five species (Hydropotes inermis, Felis catus, Capreolus pygargus, Nyctereutes procyonoides, and Canis lupus familiaris) listed in number of occurrences. The observed road-kill frequency rapidly increased until April 2019 and peaked on May 2019. However, as the system is just starting, the results from KROS cannot be treated as the exact representation of road-kill trend in the country. Although the efficient method to manage national road-kill statistic is arranged, still there are some limitations to overcome to make the system stable.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.2
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• pp.1-8
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• 1999

This study is one of the processes to build the artificial wetlands in the urban area. The purpose of this study was to survey biotic or physical environments, and to review modelling techniques to find out the ecological structure and function. Case study site was the Bangdong wetland in the north west side of Taejon metropolitan city. The number of species of insects decreased during monitoring. But the number of individuals of insects decreased abruptly after increasing. And then biodiversity index dropped and dominance index increased. So the structure of biotopes was affected by weather conditions. And in the restricted area such as urban area, the changes of insects were affected sensitively by not physical but environmental changes. As for birds, the number of species was reduced a little, but the number of individuals increased abruptly. And dominance index increased slowly. The changes of water depth and increase of temperature affected the habitat condition of vegetations and birds, so the dominance index of specific species increased. In urban areas it is necessary to continue management for water environmental changes, such as the depth and area of water. The number of species of fishes was reduced a little, but the number of individuals increased abruptly. And dominance index and biodiversity index decreased slowly. But the water environments was so changeable that it is difficult to explain current status as some specific trends. The number of species of reptiles and amphibians changed little, but the number of individuals increased abruptly. And dominance and biodiversity were not changed. The changes of water depth and temperature affected the habitat of every species the environmental changes affected.

• Korean Journal of Ecology and Environment
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• v.52 no.4
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• pp.358-365
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• 2019

We measured the body composition, including bone mineral density (BMD) and food resource availability using dual energy X-ray absorptiometry and the carbon and nitrogen stable isotope ratios of body condition of 4 anuran species(Hyla japonica, Glandirana rugosa, Pelophylax nigromaculatus, Lithobates catesbeianus) in South Korea. Additionally, the carbon and nitrogen stable isotope ratios were employed to determine the food resource availability of anurans. We figured out the relationship between the body composition, including BMDs and food resource availability. The body composition and BMDs did not differ between male and female while there was difference among the species. Food resource availability and BMDs were the highest in L. catesbeianus, the lowest in H. japonica. BMDs tended to increase with higher food resource availability. Body composition and BMDs, which represent the body condition of an individual, can be used as an ecological indicator to assess the stability of the habitat of anurans.

• Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.22-30
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• 2011

Vitellogenin (Vg) is the precursor of vitellin (Vn), which is the major yolk protein in nearly all oviparous species, including fish, amphibians, reptiles, and most invertebrates. It is one of the most important factors during reproduction, and numerous studies have shown that Vg genes are markers of the reproductive cycle and effecter genes induced by endocrine-disrupting chemicals (EDCs). Previously, we isolated two distinct cDNAs encoding vitellogenin homologs Pj-Vg1 and Pj-Vg2 from Pandalus shrimp Pandalopsis japonica. In this study, full-length genomic sequences of Pj-Vg1 and Pj-Vg2 were determined using a PCR-based genome walking strategy. Isolated Pj-Vg1 and Pj-Vg2 genes were 11,910 and 11,850 bp long, respectively. Both Pj-Vg genes had 15 exons and 14 introns, and the splicing sites were also the same, suggesting that they arose via gene duplication. The similar structural characteristics of decapod Vg genes suggest that they are all orthologs that evolved from the same ancestral gene. Analysis of Pj-Vg1 and Pj-Vg2 expression revealed that the relative copy numbers of Pj-Vg1 and Pj-Vg2 were similar in the hepatopancreas, whereas Pj-Vg2 transcripts were also detected in the ovary. Expression of both Pj-Vg genes was induced in hepatopancreas of mature individuals, whereas only Pj-Vg2 transcripts were upregulated in the ovaries from mature animals, suggesting that both Pj-Vgs are important for oocyte development. A strong positive correlation was found between Pj-Vg1 and Pj-Vg2 transcripts in the same individual, indicating they are under the same control mechanisms. Additionally, a positive correlation was found between ovarian and hepatopancreatic Pj-Vg2 transcripts, suggesting that its dual expression is regulated by similar physiological conditions. Knowledge of the similarities and differences between the two vitellogenin-like genes, Pj-Vg1 and Pj-Vg2, would help us to understand their roles in reproduction and other physiological effects.