• Toxicological Research
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• 제34권2호
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• pp.143-150
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• 2018

It has been demonstrated that vanadate causes nephrotoxicity. Vanadate inhibits renal sodium potassium adenosine triphosphatase (Na, K-ATPase) activity and this is more pronounced in injured renal tissues. Cardiac cyclic adenosine monophosphate (cAMP) is enhanced by vanadate, while increased cAMP suppresses Na, K-ATPase action in renal tubular cells. There are no in vivo data collectively demonstrating the effect of vanadate on renal cAMP levels; on the abundance of the alpha 1 isoform (${\alpha}_1$) of the Na, K-ATPase protein or its cellular localization; or on renal tissue injury. In this study, rats received a normal saline solution or vanadate (5 mg/kg BW) by intraperitoneal injection for 10 days. Levels of vanadium, cAMP, and malondialdehyde (MDA), a marker of lipid peroxidation were measured in renal tissues. Protein abundance and the localization of renal ${\alpha}_1-Na$, K-ATPase was determined by Western blot and immunohistochemistry, respectively. Renal tissue injury was examined by histological evaluation and renal function was assessed by blood biochemical parameters. Rats treated with vanadate had markedly increased vanadium levels in their plasma, urine, and renal tissues. Vanadate significantly induced renal cAMP and MDA accumulation, whereas the protein level of ${\alpha}_1-Na$, K-ATPase was suppressed. Vanadate caused renal damage, azotemia, hypokalemia, and hypophosphatemia. Fractional excretions of all studied electrolytes were increased with vanadate administration. These in vivo findings demonstrate that vanadate might suppress renal ${\alpha}_1-Na$, K-ATPase protein functionally by enhancing cAMP and structurally by augmenting lipid peroxidation.

• 한국대기환경학회지
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• 제17권5호
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• pp.407-414
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• 2001

In this study, the absorption kinetics of $CO_2$onto a mixture of AMP (2-amino-2-methyl-1-propanol) MEA (monoethanolamine) water were investigated at 30 and 4$0^{\circ}C$ using a packed absorption tower. Solubility and absorption rate of $CO_2$into alkanolamine solutions and optimal condition of $CO_2$absorption process were investigated. The experimental conditions are as follows; temperature of 30 and 4$0^{\circ}C$, gas flow rate of 3ι/min for the absorption tower, and liquid flow rate of 0.1ι/min. Feed gas was a mixture of 85% $N_2$and 15% $CO_2$. The experimental results showed that AMP had greater solubilities and faster absorption rates than MEA and DEA. In addition, MEA had the fastest initial reaction rate. To improve the properties of AMP which have low initial reaction rate and high cost, AMP was used with MEA. The mixing ratio was also changed in constant total molarity of 1,2,3 and 4. The experimental results can be summarized as follows: (1) in solubility experiment, the addition of MEA in constant total polarity decreased the solubility of $CO_2$in AMP/MEA mixture. (2) from 0 to about 0.3 in mixing ratio, the solubility of $CO_2$in AMP/MEA mixture had little differences compared with the sum of solubility of AMP only and solubility of MEA only . (3) mixing ratio of 0.3 was found to be an optimal point with the fastest $CO_2$absorption rate.

• 한국수산과학회지
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• 제8권3호
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• pp.171-176
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• 1975

개불, Urechis unicinctus, 건조중의 핵산관계물질의 변화를 음이온교환칼럼크로마토그래피로 실험하여 다음과 같은 결과를 얻었다. 살아있는 개불에는 AMP가 건물량기준으로 $6.8\mu\;mole/g$ 로서 가장 함량이 높았고, IMP는 존재하지 않았다. 건조중 ATP, ADP, AMP는 감소하고 inosine과 hypoxanthine은 증가하였으나, AMP이외의 다른 pattern은 함량이 적고 개불에서와 마찬가지로 건조한 개불에 있어서도 AMP가 건물량기준으로 $5\mu\;mole/g$로서 함량이 가장 많았다.

• 한국전자통신학회논문지
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• 제11권7호
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• pp.665-670
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• 2016

본 논문에서는 IoT(Internet of Things) 시스템의 기본 구성이 되는 센서 네트워크에 사용될 수 있는 MOSFET(Metal Oxide Semiconductor Field Effect Transistor)의 Sub-threshold 동작을 이용하는 OP-AMP(Operational amplifier) 설계를 제안한다. MOSFET의 Sub-threshold 동작은 전원전압을 낮추는 효과로 회로 시스템을 초저전력으로 유도할 수 있는 특징이 있기 때문에 배터리를 사용하는 IoT의 센서 네트워크 시스템의 초저전력화에 매우 유용한 회로설계 기술이라고 할 수 있다. $0.35{\mu}m$ 공정을 이용한 시뮬레이션 결과, VDD를 0.6 V로 설계할 수 있었으며, OP-AMP 의 Open-loop Gain은 43 dB, 또한 설계한 OP-AMP의 소비전력은 $1.3{\mu}W$가 계산되었다. 또한, Active Layout 면적은 $64{\mu}m{\times}105{\mu}m$이다. 제안한 OP-AMP는 IoT의 저전력 센서 네트워크에 다양한 응용이 가능할 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.181-186
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• 2009

In our previous report, we demonstrated that the tyrosine phosphorylation of sperm proteins (TPSP) of guinea pig was associated with capacitation and hyperactivation (CAHA), and $Ca^{2+}$ and ${HCO_3}^-$ were required for the initiation of CAHA and increasing the TPSP. The aim of this study was to further investigate the mechanism underlying the above events. The results showed that addition of cAMP agonists, dibutyryl-cAMP (db-cAMP) and isobutyl-methylxantine (IBMX), to ${HCO_3}^-$ -free medium significantly increased CAHA to the normal level (when sperm were incubated in TALP). Although addition of the cAMP agonists to $Ca^{2+}$-free medium increased CAHA, the percentages of hyperactivated and capacitated sperm were still significantly lower than the normal level. Compared with ${HCO_3}^-$ -free or $Ca^{2+}$-free medium, TPSP was increased when db-cAMP and IBMX were added in the media. H-89, a specific inhibitor of protein kinase A (PKA), inhibited CAHA in a dose-dependent manner and totally blocked TPSP. These results confirm a previous observation that $Ca^{2+}$ and ${HCO_3}^-$ regulated CAHA and TPSP in a cAMP/PKA pathway, and support an interation between TPSP and CAHA of sperm. Besides the cAMP/PKA pathway, $Ca^{2+}$ might have also played a role in regulating CAHA by other pathways since the normal level of CAHA did not recover by adding cAMP agonists in the media.

• The Korean Journal of Physiology and Pharmacology
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• 제3권2호
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• pp.157-164
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• 1999

To determine molecular mechanisms of Aquaporin-CD (AQP2) gene regulation, the promoter region of the AQP2 gene was examined by transiently transfecting a promoter-luciferase reporter fusion gene into mouse renal collecting duct cell lines such as mIMCD-3, mIMCD-K2, and M-1 cells, and NIH3T3 mouse embryo fibroblast cells. PCR-Southern analysis reveals that mIMCD-3 and mIMCD-K2 cells express AQP2, but M-1 and NIH3T3 cells do not, and that the treatment with cpt-cAMP $(400\;{\mu}M)$) or forskolin/isobutylmethylxanthine (IBMX) increased the AQP2 expression in IMCD cells. In both IMCD and NIH3T3 cells, the constructs containing the promoter of AQP2 gene showed promoter activities, indicating lack of tissue-specific element in the 1.4 kb 5'-flanking region of the mouse AQP2 gene. Luciferase activity in the IMCD cells transfected with the construct containing 5-flanking region showed responsiveness to cpt-cAMP, indicating that the 1.4 kb 5'-flanking region contains the element necessary for the regulatory mechanism by cAMP. The promoter-luciferase constructs which do not have a cAMP-responsible element (CRE) still showed the cAMP responsiveness in IMCD cells, but not in NIH3T3 cells. Increase in medium osmolarity did not affect AQP2 promoter activity in mIMCD-K2 cells. These results demonstrate that AQP2 gene transcription is increased with cAMP treatment through multiple motifs including CRE in the 5'-flanking region of the gene in vitro, and the regulatory mechanism may be important for in vivo regulation of AQP2 expression.

• 대한약리학회지
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• 제31권2호
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• pp.219-231
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• 1995

Vasoactive intestinal polypeptide(VIP) and ${\beta}-adrenergic$ agonists have immunomodultory effects on the peripheral blood T-lymphocytes of rat through their own receptors. Both of them utilize the same signal transduction pathway. That is, the stimulatory guanine nucleotide binding protein(G protein) mediates the receptor-adenylyl cyclase coupling, producing intracellular increase of cyclic adenosine monophosphate(cAMP). In the previous experiment, propranolol, a ${\beta}-adrenergic$ receptor blocker, inhibited the VIP-induced protein phosphorylation in lymphocytes. However, propranolol could not block the effect induced by forskolin. Therefore, this study was designed to elucidate the mechanism of the inhibitory action of propranolol on the effects of VIP. Using peripheral blood lymphocytes of rats, the effect of propranolol on the receptor binding characteristics of VIP was observed. And the effects of propranolol were compared to the effects of timolol on the cAMP increase induced by isoproterenol, VIP or forskolin. The results obtained are as follows. 1) Receptor binding study showed no significant differences in the affinity or density of VIP receptor between the control and propranolol-pretreated groups. 2) VIP-induced increase of cAMP was inhibited by propranolol, but not by timolol. 3) Both propranolol and timolol suppressed the isoproterenol-induced cAMP increase. 4) Propranolol also inhibited the histamine-induced cAMP increase. 5) Propranolol did not inhibit the increase of cAMP stimulated by forskolin. 6) Lidocaine did not block the VIP-induced cAMP increase. These results show that the inhibitory mechanism of propranolol is not related to ${\beta}-adrenergic$ receptor or its membrane stabilizing effect, and it is suggested that propranolol can block the effects of VIP by inhibiting the intermediate step between the VIP receptor and adenylyl cyclase.

• Journal of Radiation Protection and Research
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• 제23권2호
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• pp.115-121
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• 1998

본 연구에서는 AMP와 $MnO_2$에 대한 해수중 방사성 핵종 및 안정원소들의 흡착율을 검토하였다. $^{137}Cs$, $^{85}Sr$ 및 $^{131}I$에 대한 AMP의 흡착율은 각각 97.5%, 11.8% 및 15.1%를 나타낸 반면, 기타 다른 핵종 및 원소에 대한 흡착율은 6% 미만이었다. $MnO_2$에 대한 흡착율은 $^{40}K$, $^{137}Cs$ 및 $^{203}Hg$를 제외한 대부분의 방사성핵종 및 안정원소에 대해 90% 이상이였으나, $^{40}K$, $^{137}Cs$ 및 $^{203}Hg$의 흡착율은 8% 미만이였다.

• Bulletin of the Korean Chemical Society
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• 제15권5호
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• pp.394-399
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• 1994

The catalytic mechanism of malonyl-CoA synthetase from Rhizobium trifolii was investigated by the steady state kinetics and intermediate identification. Initial velocity studies and the product inhibition studies with AMP and PPi strongly suggested ordered Bi Uni Uni Bi Ping-Pong Ter Ter system as the most probable steady state kinetic mechanism of malonyl-CoA synthetase. Michaelis constants were $0.17{\pm}0.04 {\mu}M,\;0.24{\pm}0.18 {\mu}M\;and\;0.045{\pm}0.26 {\mu}$M for ATP, malonate and CoA, respectively. The TLC analysis of the $^{32}P-labelled$ products in reaction mixture containing $[{\gamma}-^{32}P]$ ATP in the absence of CoA showed that PPi was produced after the sequential addition of ATP and malonate. Formation of malonyl-AMP, suggested as an intermediate in the kinetically deduced mechanism, was confirmed by the analysis of $^{31}P-NMR$ spectra of AMP product isolated from the $^{18}O$ transfer experiment using $[^{18}O]$malonate. Two resonances were observed, corresponding to AMP labelled with zero and one atom of $^{18}O$, indicating that one atom of $^{18}O$ transferred from $[^{18}O]$malonate to AMP through the formation of malonyl-AMP. Formation of malonyl-AMP was also confirmed through the TLC analysis of reaction mixture containing $[{\alpha}-^{32}P]$ATP. These results strongly support the ordered Bi Uni Uni Bi Ping-Pong Ter Ter mechanism deduced from the initial velocity and product inhibition studies.

• 해양환경안전학회지
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• 제29권2호
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• pp.215-222
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• 2023

세계적 선박 배출가스 저감에 대한 규제로 인해 항만 및 부두에서 육상전원공급장치(AMP, Alternative Marine Power)가 지속적으로 사용되고 있다. 국내 또한 항만 대기질 개선 특별법에 따라 AMP의 사용이 의무화되고 있고, 다양한 친환경선박과 연계하여 활용하도록 지속적인 시도를 하고 있다. 본 논문에서는 AMP를 통해 선내 전력을 공급하는 상황을 모델링 하여 AC Load Flow Analysis를 수행하였다. 이를 통해 선박에 전력이 공급될 때의 전기적 파라미터와 손실 등에 대해서 분석할 수 있다. 아울러 Transient Stability Analysis를 통해 계통의 사고 발생 시 선내 발전기를 통한 무정전전환에 대한 HSGT(High Speed Generation Transfer)의 한계값을 도출하고 이를 적용하였을 경우 결과에 대해 검토하였다.