• Applied Chemistry for Engineering
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• v.28 no.1
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• pp.42-49
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• 2017

To replace phthalate plasticizer for PVC, acetylated monoglyceride (AMG) plasticizers were prepared from plant oil and their plasticization effects were also investigated. Transesterification of coconut oil by glycerol followed by acetylation with acetic anhydride gave AMG-CoCo (Coco : Coconut Oil). In addition, AMG-GMO (GMO : Glycerol monooleate) and AMG-GMO-Epoxy were synthesized by acetylation and epoxidation with glycerol monooleate. It was found that the thermal stability of AMG plasticizers increased in the following order: AMG-GMO-Epoxy > AMG-GMO > AMG-CoCo and all three plasticizers were thermally more stable than those of common petroleum-based plasticizer DOP (Dioctyl phthalate). The tensile strain values of the PVC containing AMG compounds were ca. 770~810%, while tensile strength values were ca. 19~22 MPa, which were higher than those of PVC containing DOP. DMA (Dynamic Mechanical Analysis) results showed that the miscibility of AMG-GMO-Epoxy in PVC was excellent and the $T_g$ of PVC containing AMG-GMO-Epoxy at 50 phr decreased down to $24^{\circ}C$. Finally, the leaching experiment result showed that the weight loss values of PVC containing AMG-GMO and AMG-GMO-Epoxy at 50 phr were as low as 2 and 1%, respectively, indicating that they have high water migration resistance. The above findings suggested that AMG-GMO-Epoxy could be one of plant oil-based PVC plasticizers to replace DOP.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.55-55
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• 2018

본 연구는 상추 '피델'을 이용한 완전제어형 식물공장에서의 재배를 하기 위해 기존의 배양액에 생육 촉진용 AMG의 농도를 구명하고 그에 맞는 매뉴얼을 확립하기 위해 실시하였다. 실험은 기존 배양액에 AMG(고활성 칼슘용액+복합 활성 미네랄)를 추가하였으며 '피델'의 생육에 적합한 AMG의 농도는 0, 0.05, 0.1, 0.2%를 양액에 추가하여 5반복 4처리로 진행하였다. 생육 조사 항목은 초폭, 초장, 엽장, 엽폭, 엽록소, 생체중을 조사하였다. '피델'의 생장에서 초폭, 엽폭, 엽장은 3주차부터 생장량이 급증하였으며 AMG 4처리구 5주차 조사에서는 초폭 평균 335g, 357g, 369g, 381g을 나타냈으며 엽폭은 평균 168g, 191g, 202g, 208g, 엽장 평균 129g, 134g, 144g, 144g으로 조사되었다. AMG 농도 0.1~0.2%는 통계적으로 생장 촉진 효과가 나타났으나 유의차가 크지 않아 0.1% AMG가 적정한 것으로 판단되었다. 엽록소 함량의 경우 정식 2주차 이후서부터는 SPAD 평균 29~32로 기록되어 엽록소함량은 크게 변화하지 않았다. 또한 엽수의 증가폭에 비해 생체중의 증가폭이 컸으며 생체중의 경우 정식 3주부터 5주 사이에 생체중이 5배 이상 급증하는 생장 패턴을 나타내었다. 5주차 생체중에서는 0% AMG의 경우 평균 82g, 0.05% AMG는 평균 85g, 0.1% AMG는 평균 115g, 그리고 0.2% AMG는 평균 114g으로 조사되었다. 따라서 0.1~0.2% AMG에서는 차이가 없게 나타남에 따라 생장촉진을 위해서는 0.1% AMG를 추가하는 것이 적정하다고 판단되었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.57-57
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• 2018

본 연구는 상추 '피델'을 이용한 완전제어형 식물공장에서의 재배를 하기 위해 기존의 배양액에 생육 촉진용 AMG의 농도를 구명하고 그에 맞는 매뉴얼을 확립하기 위해 실시하였다. 실험은 기존 배양액에 AMG(고활성 칼슘용액+복합 활성 미네랄)를 추가하였으며 '피델'의 생육에 적합한 AMG의 농도는 0, 0.05, 0.1, 0.2%를 양액에 추가하여 5반복 4처리로 진행하였다. 생육 조사 항목은 초폭, 초장, 엽장, 엽폭, 엽록소, 생체중을 조사하였다. '피델'의 생장에서 초폭, 엽폭, 엽장은 3주차부터 생장량이 급증하였으며 AMG 4처리구 5주차 조사에서는 초폭 평균 335g, 357g, 369g, 381g을 나타냈으며 엽폭은 평균 168g, 191g, 202g, 208g, 엽장 평균 129g, 134g, 144g, 144g으로 조사되었다. AMG 농도 0.1~0.2%는 통계적으로 생장 촉진 효과가 나타났으나 유의차가 크지 않아 0.1% AMG가 적정한 것으로 판단되었다. 엽록소함량의 경우 정식 2주차 이후서부터는 SPAD 평균 29~32로 기록되어 엽록소함량은 크게 변화하지 않았다. 또한 엽수의 증가폭에 비해 생체중의 증가폭이 컸으며 생체중의 경우 정식 3주부터 5주 사이에 생체중이 5배 이상 급증하는 생장 패턴을 나타내었다. 5주차 생체중에서는 0% AMG의 경우 평균 82g, 0.05% AMG는 평균 85g, 0.1% AMG는 평균 115g, 그리고 0.2% AMG는 평균 114g으로 조사되었다. 따라서 0.1~0.2% AMG에서는 차이가 없게 나타남에 따라 생장촉진을 위해서는 0.1% AMG를 추가하는 것이 적정하다고 판단되었다.

• Korean Journal of Plant Resources
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• v.32 no.3
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• pp.228-236
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• 2019

This study is conducted to investigate the effects of growth characteristics (GC) and DPPH radical scavenging activity (DRSA) of the heading lettuce 'Fidel' depending on four concentrations of activated mineral groups (AMG: 1.6% active minerals and 0.03% CaO) added to basic nutrient solutions (diluted by 0, 0.05, 0.1, and 0.2%) and on four different light-emitting diodes (LED; B:R:W ratio = 0:1:12, 0:1:9, 0:5:5, and 2:3:5). Both 0.1 and 0.2% AMG showed better GC of lettuce in plant width, plant length, leaf width, leaf length, and the number of leaves than those of other AMG, while leaf thickness and chlorophyll value did not show significant difference among all AMG. Moreover, 0.1 and 0.2% AMG showed heavier shoot fresh weights than those of other AMG. As for the combinations of AMG and LED, B0:R5:W5 showed the best lettuce GC regardless of AMG compared to other AMG and LED combinations. As shown in the above results of LED, although there was a difference in lettuce growth by LED, the differences of lettuce growth by AMG were statistically significant. The DRSA was the highest at 82.8% in B2:R3:W5, followed by B0:R5:W5 at 77.8%. LED showed differences but AMG did not affect DRSA. Therefore, the optimal conditions in plant factory for GC and DRSA of the lettuce were 0.1% AMG and B0:R5:W5.

• Applied Microscopy
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• v.40 no.1
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• pp.15-19
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• 2010

The present study was designed to demonstrate ionic zinc in the rat DRG by means of zinc selenium autometallography($ZnSe^{AMG}$). Ganglion cells varied in size from 15 to 100 ${\mu}m$. The smaller neurons were strongly stained with AMG, whereas the larger cells were weakly stained. Each large ganglion cell was surrounded by perineuronal satellite cells, showing apparent AMG staining. We demonstrated for the first time the existence of zinc-containing satellite cells in the rodent DRG. Using electron microscopy, fine AMG grains were observed scattered in the somata of the DRG neurons, especially small cells. However, much lower concentrations of the AMG grains occupied in the large cells, and these were mostly localized in lysosome-like organelles. These results indicate that zinc may be involved in sensory transmission in the DRG level.

• Journal of Soil and Groundwater Environment
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• v.8 no.3
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• pp.13-22
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• 2003

Laboratory scale study for an air injection and flowing aspect of groundwater saturated zone was conducted for three sediment grains (AMG 0.34, 1.38, 3.89 mm diameter). Air flow for AMG (Average Modal diameter Grains) 0.34 mm diameter grain size provides indication of pattern of channelized air flow in saturated zone and expansion state in above saturated zone. Maximum area of influence is approximately l5.2%/$\textrm{m}^2$for AMG of 0.34 mm diameter. For AMG of 1.38 mm and 3.89 mm modal diameter grains, air flow are pervasive air flow, forming a symmetrical cone of influence around the injection point. Maximum areas affected are 37%/$\textrm{m}^2$for AMG 1.38 mm diameter and 30%/$\textrm{m}^2$for AMG 3.89 mm diameter. AMG 1.38 mm and 3.89 mm diameter grains show onset of collapse and approach to steady state in above saturated zone, respectively. In this study, optimal sites for in situ air sparging, may be grain diameters between about AMG 1.5-2.5 mm diameter.

• Applied Microscopy
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• v.33 no.2
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• pp.145-154
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• 2003

The present study was designed to demonstrate ionic zinc in the rat nasal mucosa by means of zinc selenium autometallography ($ZnSe^{AMG}$). Rats were given sodium selenide either intraperitoneally (i.p) or intranasally (i.n). Prior to the i.n. administration the rats were anesthetized with pentobarbital sodium (30 mg/kg, i.p.). A thin plastic tube coupled to a Hamilton syringe was then inserted into the right nostril and $10{\mu}l$ of the solution was instilled. For the i.p. administration non-anesthetized rats were given $100{\mu}l$ of the sodium selenide solution (10 mg/kg). Control rats were instilled with saline. After 2 hrs survival, the rats were anaesthetized and transcardially perfused with 3% glutaraldehyde. The olfactory area was removed and put into same fixative. The nose was then sectioned ($30{\mu}m$) horizontally, autometallography (AMG) was performed according to Danscher et al. (1997). After silver enhancement, fine AMG grains were scattered in the whole length of the olfactory epithelium containing olfactory receptor neurons, sustentacular and basal cells. However, much higher concentration of the AMG grains occupied near the surface and in the basal region of the olfactory epithelium. Both groups of i.p. and i.n. administration showed almost same level in the concentration of the AMG grains. In i.n. group, few AMG grains were also found in olfactory nerves of the lamina propria, suggesting zinc transport into the olfactory bulb via olfactory axons. At the electron microscopic level, the AMG grains were most entirely found in the supporting cells of the olfactory epithelium, and they were mostly localized in lysosome-like organelles. The i.n. group showed various signs of tissue damage of the olfactory mucosa, where dense concentration of AMG grains were localized at crystalloid structures. The present study demonstrated dense population of ionic zinc in the rat olfactory epithelium. zinc may play a role in the olfactory functioin and in the pathogenesis of the neurodegerative disorders affecting nose.

• KSBB Journal
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• v.16 no.5
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• pp.459-465
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• 2001

The on-line monitoring technique for the concentrations fo glucose and starch by FIA(Flow Injection Analysis)system was studied. Glucose oxidase(GOD) and amyloglucosidase(AMG) were immboilized on VA-Fpoxy carrier and integrated into the FLA system. The pH, buffer flow rate and temperature were optimized and the effects of salts and metabolites dissolved in the sample on the activity of immobilized enzyme were investigated. GOD-FIA and AMG/GOD-FIA were applied for the on-line monitoring of the glucose and starch in a simulated bioprocess. The on-line measurements of glucose concentrations by GOD-FIA agreed with off-line data well and the AMG/GOD-FIA with single cartidge system took and advantage over the FIA system with two separated cartridges for the on-line monitoring of starch concentrations.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.213-217
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• 2004

Laboratory scale study for an area of influence and flowing aspect of groundwater saturated zone was conducted for three sediment grains. On the AMG(Average Modal diameter Grains) 0.34, 1.38, 3.89mm diameter samples, the affected area of the aquifer were 15.2, 37.0, 30.0%/m2 each. Air flow for AMG 0.34mm diameter grain size provides indication of pattern of channelized air flow in saturated zone and expansion state in above saturated zone. For AMG of 1.38, 3.89mm diameter grains, air flow are pervasive air flow, forming a symmetrical cone of influence around the injection point. And also AMG 1.38, 3.89mm diameter samples show onset of collapse and approach to steady state in above saturated zone, respectively. In this study, optimal sites for in situ air sparging, may be grain diameters between about AMC 1.5～2.5mm diameter.

• Applied Microscopy
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• v.30 no.4
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• pp.357-365
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• 2000

Paneth cells have been suggested to contribute to the elimination of excess metals into the intestinal lumen. The purpose of this study wat to investigate the changes of the zinc pools in rats subjected to functional loading with zinc salt by mean of both light and electron microscopical autometallography (AMG). Wistar rats 4 were administrated with zinc chloride (20 mg/kg body weight) intraperitoneally dissolved in 1 ml distilled water. The control group received 1 ml saline IP. After further one hour the animals were transcardially perfused with 0.4% sodium sulphide dissolved in 0.1 M PB fellowed by 3% glutaraldehyde solution for 10 minutes. Pieces of ileum were frozen with solid $CO_2$ and sectioned on a cryostat. The sections $(20{\mu}m)$ were autometallographically developed. Sections selected for EM were reembedded on top of a blank Epon block, from which ultrathin sections (100 nm) were cut. The ultrathin sections were double stained with uranyl acetate (30 min) and lead citrate (5 min), then examined under electron microscope. Studies of comparable sections from control and zinc loaded animals with the AMG selenium method gave quite different results. The control animals demonstrated a weakly positive staining in the cytoplasm of the Paneth cells. In the electron microscope the AMG silver grains were found to be located in the cytoplasm, while the electron dense secretary granules and other cell organelles were void of staining. Few AMG grains were located at the apical surface of the Paneth cells. In sections from zinc loaded rats, the AMG grains were seen in abundance in the lumen of the Lieberkuhn crypts at light microscopic levels. At EM levels the zinc revealing silver grains were located in the cytoplasm as in the controls, but much more AMG grains were shifted into the secretary granules. Furthermore, profound AMG grains were found in the lumen of the crypts and surrounding vessels. And a few grains were seen in the endothelium. The AMG technique demonstrated a pattern of AMG grains in the Paneth cells that strongly suggests a transport of zinc ions through these cells.