• 한국식품조리과학회지
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• 제13권1호
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• pp.23-29
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• 1997

The hypoglycemic effect of fractions of methanol extract of Cassia tora was investigated in diabetic rats. Sprague-Dawley male rats weighing 180∼230 g were divided into 6 groups. Diabetes was induced in the male rats by intravenous injection of streptozotocin at a dose of 45 mg/kg dissolved in citrate buffer (pH 4.5). The diabetic animals then had plasma glucose concentration of above 300 mg/㎗. Fractions of methanol extract of Cassia tora were administered orally into the diabetic rats for 14 days after streptozotocin injection. The food intake and body weight gain were monitored and plasma levels of glucose, triglyceride, free fatty acid, HDL-cholesterol, cholesterol and ALT activity were determined. Levels of glycogen, cholesterol and triglyceride in liver were analysed. Weights of liver and kidney were lighter in all the fraction-administered groups than streptozotocin-control group. Plasma glucose level was significantly decreased by the administration of butanol fraction at 14 days. Plasma triglyceride levels were lower in chloroform, butanol and H$_2$O fraction-administered groups compared to streptozotocin-control group. Plasma cholesterol and HDL-cholesterol levels were not significantly different in all groups. Administrations of each of the four fractions have decreased plasma free fatty acid level, ALT activity and liver triglyceride levels in the diabetic rats. Liver glycogen levels in chloroform, butanol and H$_2$O fraction-administered groups were higher than streptozotocin-control group. It is suggested from the results that butanol fraction of methanol extract of Cassia tora may contain the antihyperglycemic compounds.

• Applied Microscopy
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• 제31권1호
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• pp.37-47
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• 2001

화상에 의한 피부 손상이 간에 미치는 영향을 알아보기 위하여 흰쥐를 이용하여 피부 화상을 유도한 다음 각각 5시간, 24시간 후 생화학적 정량법과 형태학적 관찰을 통해 간 조직 손상의 발병기전을 검토해 보고자 하였다. 흰쥐의 화상 유발은 등쪽면의 털을 깎고(total burn surface area $20\sim25%$) $100^{\circ}C$ 물로 10초간 흡입손상 없이 피부 화상만을 가하였다. 생화학적 정량으로는 혈청 내 xanthine oxidase(XO)와 aniline aminotransferase (ALT)의 활성변화, 그리고 혈장 단백질 함량 변화를 측정하였고, 형태학적 관찰은 혈액 중 다형핵 백혈구 수의 산정과 간 세포의 미세구조 변화를 관찰하였다. 실험 결과, 화상 후 혈청 내 XO의 활성 증가(P<0.01)와 함께 체중 당 간 무게(p<0.05)와 혈청 내 ALT의 활성이 증가되었다. 화상 직후 allopurinol의 복강투여로 XO활성, 간 무게, 그리고 ALT의 활성은 모두 감소되었다. 화상 손상에 의한 간 조직의 미세구조적 변화로는 소포체 종창, 리보솜 탈락, 지방소적의 축적, 그리고 담모세관과 세포간질의 확장이 관찰되었다. 뿐만 아니라 염증세포인 호중구의 침윤과 함께 혈관 내피세포의 손상, 쿠퍼세포의 활성화, 그리고 미세융모의 손상들이 관찰되었다. 또한 혈 중 다형핵 백혈구의 수적인 변화에서 화상 유발 5시간 후에 현저히 감소되어 내부 장기에 호중구 침윤의 가능성을 알 수 있었다. 하지만 allopurinol의 투여로 이러한 미세구조의 변화를 예방할 수 있는 것으로 나타났다. 이러한 결과들을 종합해 볼 때, 피부 화상으로부터 간 손상을 유발하는데 있어서 혈 중 XO의 활성증가가 매우 핵심적인 역할을 담당하는 것으로 사료되었다.

• Journal of Acupuncture Research
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• 제19권5호
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• pp.209-218
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• 2002

Objective : This study was undertaken to examine whether Carthami Semen aquacupuncture (CSA) exerts protective effect against Hg-induced cell injury in rabbit liver. Methods : The cell injury was evaluated by ALT activity and lipid peroxidation was estimated by measuring malondialdehyde (MDA). Results : Hg caused an increase of ALT activity and lipid peroxidation in a dose-dependent-manner over concentrations of 0.1-1 mM, which were prevented by addition of 0.005% CSA. The protective effect of CSA was dose-dependent in concentration range of 0.001 to 0.01%. The increase of ALT activity and lipid peroxidation induced by 0.5 mM Hg were almost completely decreased by addition of 0.01% CSA. When the liver tissues were exposed to 0.5 mM Hg, GSH content was decreased, which was significantly restored by 0.01% CSA. 0.5 mM Hg caused decrease in the amount of total and nonprotein sulfhydryl groups, and 0.01% CSA prevented Hg-induced reduction of nonprotein sulfhydryl group but not protein sulfhydryl group. Conclusions : These results suggest that CSA exerts protective effect against Hg-induced cell injury by antioxidant action resulting from enhancement of nonprotein sulfhydryl group content including GSH in liver.

• 생약학회지
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• 제38권2호통권149호
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• pp.139-147
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• 2007

The aim of this study was to investigate the protective activity of curcumin, demethoxycurcumin and bisdemethoxycurcumin, isolated from Curcuma longa Linne, on hepatocyte injury induced by carbon tetrachloride (CCl$_4$,10 mM), t-butyl hydroperoxide (TBH, 0.5 mM) and D-galactosamine (GaIN,30 mM). Primary cultures of rat hepatocyte (18 h culture) were treated with CCl$_4$, TBH or GaIN and various concentrations (0.1, 1, 10 and 100 ${\mu}$M) of curcumin, demethoxycurcumin and bisdemethoxycurcumin CCl$_4$ significantly increased the levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The increases in LDH, ALT and AST levels were inhibited by curcumin. Demethoxycurcumin and bisdemethoxycurcumin decreased the levels of AST. Curcumin inhibited the increases in ALT and AST levels induced by TBH. The increased levels of LDH, ALT and AST induced by TBH were inhibited by bisdemethoxycurcumin. GaIN markedly increased the levels of LDH, ALT and AST. These increases were significantly inhibited by bisdemethoxycurcumin. The increase in AST level was inhibited by curcumin. These results suggest that curcumin and bisdemethoxycurcumin have potent hepatoprotective activities.

• 한국환경과학회지
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• 제12권6호
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• pp.599-603
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• 2003

This study investigated the effects of Houttuynia Cordata thunb(HCT) administration on the biochemical parameters of function in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated rats. Houttuynia Cordata thunb (200mg/kg) was administeres into rats intraperitoneally for four weeks , seven days after the injection of TCDD(1$\mu\textrm{g}$/kg). We examined the antioxidative enzymatic activity by measuring the level of AST, ALT , SOD and Catalase in serum and liver tissue of rats. HCT group showed 49% of inhibitive effect in AST activity compared to TANO group. ALT level of HCT group was decreased to the level of NO group. SOD and Catalase in TANO group were lower than in NO group, but SOD and Catalase in HCT group were increased by 46％ and by 50％ respectively compared to TANO group.

• 약학회지
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• 제33권1호
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• pp.54-63
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• 1989

Examination of the subacute toxicity of captafol showed that. 1) In the captafol administered group, the body weight was significantly decreased but the amounts of AST, ALT, LDH, BUN, TG in serum were remarkably elevated in comparision to those of the control group. 2) In captafol treated animals, the amount of cytochrome P-450 and the activity of NADPH-cytochrome c reductase in liver and in kidney were decreased, but TAB value in serum and in liver and total ATPase activity in liver and in kideny were found to be remakably elevated. 3) When captafol administered with ethanol to the group, the group showed elevated serum levels of AST, ALT and BUN but the amount of cytochrome P-450 and the activity of NADPH-cytochrome c reductase in liver and in kidney were decreased as the group which was treated with captafol only.

• 동아시아식생활학회지
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• 제6권3호
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• pp.289-294
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• 1996

To evaluate an effect of acute ethanol pretreatment on the liver injury in toluene-treated rats, toluene(50% in olive oil) was intraperitoneally given four times at 0.3ml/100g body weight at interval of one day to the ethanol-pretreated rats(0.3ml of 50%/100g body weight). The increasing rate of liver weitht per body weight(%), serum levels of alanine aminotransferase (ALT) activity, liver lipid peroxide content was higher in toluene treated rats pretreated with ehtanol than those treated with toluene alone. Concomitantly the hepatic glucose-6-phosphatase activity was increased whereas glutathione content was decreased by ehtanol pretreatment before toluene administration in rats. In case of direct administration of acetaldhyde or benzaldehyde to the rats, the liver weitht per body weight(%) and serum levels of ALT activity were almost higher than the control group. There results indicate that the toluene treated rats showed the reversible injury of liver and intensified. however, by the ethanol treatment.

• 한국자원식물학회지
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• 제36권3호
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• pp.198-206
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• 2023

The effectiveness of the extracts of Alnus japonica and Portulaca oleracea, which are effective in improving alcohol-induced liver damage, was confirmed using acute and chronic alcoholic liver injury animal models. In the acute alcoholic liver injury model, dieting Alnus japonica and Portulaca oleracea complex (ALPOC) at a dose of 50 mg/kg showed no significant change in liver or body weight, while measured plasma ALT activity to be deficient (28.12 U/ml) compared to the alcohol intake group (42.5 U/ml), and confirmed that restored it to an average level. It showed an improvement of 34.9% compared to the alcohol intake group. AST activity confirmed that it showed a very effective liver protection activity by showing a gain of 12.6%. The chronic alcoholic liver damage animal model demonstrated that ALT showed an improvement effect of 25%, and AST showed an effect similar to that of the positive control group, Hovenia extract. In addition, through H&E staining analysis, observed that the ALPOC improved the necrosis and bleeding of the liver. And the ALPOC group showed intense antioxidant activity of 127% or more compared to the alcohol intake group, and this was confirmed to have a very high activity, which is more than 20% higher than that of the hovenia fruit extract.

• 한국자원식물학회지
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• 제25권5호
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• pp.633-639
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• 2012

시로미 열매 추출물의 라디칼 소거 활성과 간 손상 동물모델에서의 항산화 효소 활성 측정을 통해 추출물의 항산화 효과를 평가하였다. In vitro 실험에서, 시로미 열매 추출물은 12.0 ug/ml의 농도에서 DPPH 라디칼을 50% 소거하는 활성을 보였고, 1 mg/ml의 농도에서 56.1%의 지질과산화 억제 활성을 나타내었다. 그리고 $CCl_4$로 유도한 간 손상 동물모델에서 serum ALT 및 AST의 수치를 감소시켜 간보호 효능을 나타내었고, 간 조직의 지질 과산화를 최대 73% 억제하였으며, 항산화 효소인 SOD 및 catalase의 활성을 유의적으로 증가시켰다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제12권1호
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• pp.30-38
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• 2009

목 적: 비만에서 발생하는 비알코올성 지방간질환은 대부분 무증상이어서 혈청 검사에서 간효소치 상승이 있거나 간초음파검사에서 지방간이 발견되는 경우에 이 질환에 대한 의학적 관심이 증가한다. 본 연구에서는 비만아와 과체중아에서 간세포손상을 반영하는 ALT 상승과 신체 계측치 및 생화학적 검사치들과의 관련성을 알아보고자 하였다. 방 법: 2006년 1월부터 2008년 6월까지 충남대병원 소아청소년과에서 비만 또는 과체중으로 진단받은 142예를 대상으로 신체 계측, 생화학 검사를, 간초음파검사는 일부 환자에서 시행하였다. ALT치를 기준으로 정상 군(I군)과 상승 군(II군)으로 분류하여 신체 계측치, 생화학 검사치, 간초음파 소견을 비교하였다. 바이러스간염, 약물 유발 간염, 대사이상 간염, 내분비 장애, 유전 질환이 있는 경우는 연구 대상에서 제외하였다. 결 과: 단순 비만아에서 간전이효소치의 상승은 전체의 54.2%에서 있었으며, 남녀별로는 남아 63.0%, 여아 26.5%에서 있었다. I군과 II군의 나이는 각각 11.4${\pm}$3.3, 11.7${\pm}$3.2세로 차이가 없었다. 성별 분포는 I군은 남아 40예, 여아 25예로 총 65예, II군은 남아 68예, 여아 9예로 총 77예로 양군 모두 남아가 통계적으로 유의하게 많았으며(p=0.003, 0.001) 남녀 비는 I군 1.6:1, II군 7.6:1로 II군에서 남아의 발생빈도가 4.8배 더 높았다. 신체 계측치는 허리둘레/키 비, 엉덩이둘레/키 비가 II군에서 통계적으로 유의하게 높았으며(p=0.001, 0.046), 키, 체중, 체질량지수, 허리둘레, 엉덩이둘레, 허리둘레/엉덩이둘레 비, 비만 정도는 양 군 간에 유의한 차이가 없었다. 생화학 검사치와 인슐린 저항성에서는 AST, ${\gamma}$-GT, 총콜레스테롤 수치가 II군에서 통계적으로 유의하게 높았고, AST/ALT비는 I군 1.10${\pm}$0.41, II군 0.55${\pm}$0.17로 II군에서 유의하게 낮았다(p<0.001, <0.001, <0.001). 중성지방, 고밀도지질단백 콜레스테롤, 저밀도지질단백 콜레스테롤 수치, 공복 시 인슐린 농도, 혈당, 인슐린 저항성 지표는 두 군 간에 통계적으로 유의한 차이가 없었다. 단변량 상관관계분석에서는 체질량지수, 엉덩이둘레/키 비, 허리둘레/키 비, ${\gamma}$-GT, 중성지방이 양의 상관관계를 보였다. 간초음파검사는 I군 11예, II군 32예에서 시행되었는데, 양 군 모두 경도의 지방간 소견이 가장 많았으며, 양 군 간에 지방간 빈도에는 통계적으로 유의한 차이가 없었다. 결 론: 비만아에서 허리둘레/키 비, AST, AST/ALT비, ${\gamma}$-GT, 총 콜레스테롤 농도가 ALT치가 정상인 군 보다 상승된 군에서 통계적으로 유의한 차이를 보였으며, 허리둘레/키 비와 ${\gamma}$-GT가 ALT 상승과 통계적으로 유의한 양의 상관관계를 보여 쉽게 측정할 수 있는 신체 계측치인 허리둘레/키 비를 ALT치가 상승된 비만아의 관리에 참고 자료로 이용해 볼 수 있겠다. 그러나 이에 대해서는 향후 더 많은 연구가 필요하다.