• 한국식품영양과학회지
• /
• 제27권4호
• /
• pp.698-704
• /
• 1998

This study was carried out to investigate the effects of diet of Korean safflower(Carthamus tinctorious L.) seed powder on bone tissue during the recovery of rib-fracture in rats. Male Sprague-Dawley rats of 10 weeks old, weighing 370$\pm$5g, were divided into two groups including the control group(C group, AIN-76 semipurified diet) and safflower seed group(S group, AIN-76 semipurified diet+10% safflower seed powder) and were fed experimental diets for 12 days after adaptation period. After this period, the 9th right rib was fractured surgically and sham-operation was also performed. Rats were fed with experimental diets for up to 30 more days after rib-fracture. The degree of bone repair was evaluated during the recovery period at the 8th, 11th, 16th, 21st, 30th days after the surgical operation by microscopic observation of the fractured rib tissue. In callus formation, the portion of hyaline cartilage was noticably higher in S group than C group. The intracatilagenous ossification was observed at the 8th day in S group, but at 11th day in C group. The intramembranous ossification in callus was widely found over the 8th day to the 11th day in S group, but it was shown over the 11th day to the 16th day in C group. Bone resorption was also occured more rapidly in S group as indicated by large numbers of osteoclasts observed. At the 30th day, most of trabecular bones were disappeared in S group, whereas still shwon in C group over wide ranges of fractured ribs. These results imply that the supplementation of Korean safflower seed powder influences in the recovery of bone fracture by accelerating the process of bone repair.

• Applied Biological Chemistry
• /
• 제42권1호
• /
• pp.29-33
• /
• 1999

당귀(Angelica gigas Nakai)의 첨가 식이가 만성적인 알콜 투여시 흰쥐의 지방대사, 알콜대사 및 간기능에 미치는 영향을 조사하기 위해 Sprague-Dawley 수컷 쥐를 대조군(증류수 투여), 에탄올 투여군, 당귀 첨가 식이군, 에탄올 투여 와 당귀 첨가 식이군의 4그룹으로 나누어 30일간 에탄올 및 증류수는 경구 투여하였고, 당귀는 AIN-76 식이에 첨가하여 섭취하도록 하였다. 그룹간 혈중 알콜대사 변화를 조사하기 위해 21째 날에 13% 알콜을 모든 군의 쥐에 경구 투여한 후 혈중 알콜농도 변화를 5시간 동안 조사하였으며, 그 결과는 다음과 같다: 1) 당귀 첨가 식이군의 혈중 알콜농도가 정상식이군에 비하여 알콜투여 후 2시간부터 유의적으로 감소하였다; 2) 만성적인 알콜섭취와 동시 당귀 첨가 식이군의 알콜 투여 직후의 혈중 알콜 산화 속도는 정상식이군 혹은 당귀 첨가 식이군에 비하여 전반적으로 빠르게 진행되었다. 또한 30일 후에는 각 그룹의 쥐를 희생시켜 혈중 및 간장 중의 지방성분 및 혈중의 간장 관련 효소의 활성을 측정하였으며, 그 결과는 다음과 같다: 1) 당귀 첨가 식이는 혈중 LDL-콜레스테롤 함량을 비첨가 식이군에 비하여 유의적으로 낮추었다; 2) 당귀 첨가 식이는 알콜투여로 증가된 간장 중의 중성지방 및 총지방 함량과 혈중 ${\gamma}-GTP$의 수준을 유의적으로 낮추었다. 이상의 결과로부터 당귀 첨가식이가 만성적인 알콜 섭취로 야기될 수 있는 질병에 대한 개선 효과가 있음을 제안할 수 있겠다.

• Food Science and Biotechnology
• /
• 제17권2호
• /
• pp.287-294
• /
• 2008

Protective effects of cheonggukjang fermented with 20% red ginseng (RC) were observed in streptozotocin (STZ)-induced diabetic rats by measuring levels of blood glucose, serum lipid profiles, and hepatic reactive oxygen species generating and scavenging enzymc activities. RC diet was prepared by mixing with AIN-76 diet at the final concentration 2%, and it was fed to STZ-induced diabetic rats for 3 weeks. The RC dict was significantly improved body weight, feed efficiency ratio, levels of serum glucose, and serum and hepatic lipids in diabetes. The significantly elevated O type activity of xanthine oxidase in diabetes was also greatly decreased by the RC diet. The treatment of RC showed the improved hepatic glutathione s-transferase activities in the diabetic animals. The present study indicates that cheonggukjang fermented with red ginseng could ameliorate STZ-induccd diabetic symptoms such as aggravated blood glucose levels, serum lipid profiles, and even the conditions of oxidative stress.

• Journal of Nutrition and Health
• /
• 제34권5호
• /
• pp.493-498
• /
• 2001

To investigate the effects of Ixeris sonchifolia Hance diets on serum and hepatic lipid levles and enzyme activities in rats administered with ethanol chronically, Sprague-Dawley male rats were AIN-76 diet(control), control diet plus ethanol, control plus Ixeris sonchifolia Hance diet, or control plus Ixeris sonchifolia Hance diet plus ethanol for 30 days. Ixeris sonchifolia Hance diets significantly decreased the serum total cholesterol, triglyceride, LDL-cholesterol, and GOT levels that were increased due to the chronic ethanol administration. In addition, Ixeris sonchifolia Hance diets significantly decreased the liver triglyceride and total lipid levels that were increased due to the ethanol administration. The present findings, combined with previous data showing differences in the effects of cabbage diets having a high or a low level of GABA on the lipid levels and the serum Y-GPT activity of rats(Cha and OH[2000] J. Korean Soc. Food Sci. Nutr. 29, 500-505) raise the possibility that GABA in plants could have a nytraceutical role in the recovery of chronic alcohol-related diseases. (Korean J Nutrition 34(5) : 493∼498, 2001)

• Journal of Nutrition and Health
• /
• 제29권6호
• /
• pp.590-593
• /
• 1996

This study was done to evaluate the effect of dietary calcium level (a diet which met 100% or twice the calcium level in AIN-76 diet) on preventing bone loss in ovariectomized rats. Forty Sprauge-Dawley female rats(body weight 200$\pm$5g)were divided into two groups. One group were ovariecotomized (Ovx) while the others received sham operation(Sham). Thereafter, each rat group was further divided into normal calcium diet(0.52%) and high calcium diet(1.04%) subgroups. All rats were fed on experimental diet and deionized water ad libitum for 8 weeks. The total body, spine and femur bone mineral densities and bone mineral contents were measured by Dual Energy X-ray Absorptiometry, Eight weeks following operation, ovariectomized rats fed a high calcium diet had a significantly higher total bone mineral content, total bone calcium content, spine bone mineral density, spine bone mineral content and femur bone mineral content than ovariectomized rats fed control calcium diet. The correlation between dietary calcium intake level and spine bone mineral density were positive, but there was no correlation between dietary calcium intake and femur bone mineral density. The findings from the present study demonstrated that bone loss due to ovarian hormonal deficiency can be partially prevented by a high calcium diet. Futhermore, these findings support the strategy of the use of a high calcium diet in the prevention of estrogen depleted bone loss(postmenopausal osteoporosis)

• Food Science and Biotechnology
• /
• 제16권6호
• /
• pp.879-883
• /
• 2007

This study examined whether dietary supplementation with sea tangle alters the intestinal morphology of streptozotocin-induced diabetic rats and affects the glucose absorption rate. Forty male Sprague-Dawley rats were divided into 2 groups and fed either a control (AIN76-based) diet or a sea tangle-supplemented diet. After 3 weeks, 10 rats in each group received an intramuscular injection of streptozotocin (45 mg/kg BW), and feeding was continued for 3 additional weeks. Dietary supplementation with sea tangle resulted in a lower fasting plasma glucose level compared with the control diet in diabetic rats. Scanning electron micrographs revealed serious damage to the jejunal villi of diabetic rats fed the control diet, whereas supplementation with sea tangle alleviated the damage. In a separate experiment, 20 male Sprague-Dawley rats were divided into 2 groups and fed either a control diet or a sea tangle-supplemented diet for 5 weeks, and fasted rats were subjected to in situ single-pass perfusion. The glucose absorption rate determined in the absence of digesta was decreased by 34% in the jejunum of rats fed a sea tangle diet compared with those fed a control diet. In conclusion, sea tangle supplementation lowered glucose absorption rate, altered intestinal morphology, and appeared to protect villi from damage caused by diabetes mellitus.

• 한국식품영양과학회지
• /
• 제31권1호
• /
• pp.109-116
• /
• 2002

본 연구는 김치와 노화 억제에 미치는 영향을 살펴보기 위하여 AIN-76 마우스 식이에 배추, 갓김치, 갓배추김치를 각동결건조 김치를 5%씩 첨가하여 만든 후 김치식이를 노화촉진쥐인 SAMP8에게 1년간 섭취시키면서 간의 노화관련 특성인 유리기 농도와 항산화효소계의 효소활성 변화를 살펴보았다. 노화촉진쥐 간의 총유리기, OH radical, $H_2O$$_2$그리고 TBARS 모두 가려에 따라 증가되었으며, 간에서 생성된 유리기 농도와 TBARS 농도는 대조군에 비해 김치섭취시 유의적으로 감소되었다(p<0.05). 그러나 생성된 유리기를 제거하기 위한 SOD 활성은 오히려 김치섭취군에서 유의적으로 증가되었으며(P<0.05), catalase와 GSH-px, GSH/GSSG의 비율도 김치섭취군에서 유의적으로 증가하였다(p<0.05). 따라서 김치섭취는 노화에 따라 발생되는 간의 유리기 및 TB-ARS 생성은 억제시키면서, 이를 제거하는 항산화계 효소는 활성화시켜 노화를 억제한 것으로 생각된다. 김치종류 중에서는 배추김치보다 배추갓김치와 갓김치가 유리기 생성 억제 및 항상화 효소 활성을 더욱 증진시킨 것으로 관찰되어 이는 첨가된 갓의 항산화물질 때문인 것으로 생각된다.

• 대한예방한의학회지
• /
• 제9권1호
• /
• pp.105-118
• /
• 2005

Objectives : The purpose of this study is to investigate the effects of Polygonum multiflorum THUNB(何首烏) on decresing concentration of serum lipid which are triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol. Thiobarbituric Acid Reactive(TBARS) in high cholesterol diet induced hyperlipidemia rats. Methods : Sprague-Dawley rats were divided into 3 groups, Normal group (supplied enough water and feeds only, Normal Group), high fat diet administered group(AIN-76 high fat diet #100496 used beef tallow for 4 weeks, Control Group) and Polygonum multiflorum THUNB(何首烏). administered group(AIN-76 high fat diet #100496 used beef tallow and Polygonum multiflorum THUNB(何首烏) extracts 85.0mg/200g 1time/day for 4 weeks, HSO Group). Rats were sacrificed and concentration of triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, TBARS were measured in rat's serum lipid. Results : There was no significant difference in the level of weigh between Control and HSO Group.(by Duncan test) Concentration of Total lipid has no significant difference in each group. (F=0.702, p=0.511, ANOVA test) Concentration of triglyceride has no significant difference in each group. (F=0.727, p=0.500, ANOVA test) Concentration of LDL-cholesterol was significantly different in each group.(F=.9.894. p=0.002, ANOVA test) It was significantly increased in the Control group compared with the Normal group and it significantly decreased in the HSO group compared with the Control group. Concentration of HDL-cholesterol has no significant difference in each group. (F=1.079, p=0.365, ANOVA test) TBARS values(Thiobarbituric Acid Reactive Substances) were significantly different in each group.(F=15.580, p=0.0001, ANOVA test) It was significantly increased in the Control group compared with the Normal group and significantly decreased in HSO group compared with the Control group. Conclusions : According to the above results, it is considered that Polygonum multiflorum THUNB(何首烏) is effective in decreasing concentration of serum lipid in high cholesterol diet induced hyperlipidemia rats.

• 대한예방한의학회지
• /
• 제7권2호
• /
• pp.13-22
• /
• 2003

Objectives: CRATAEGII FRUCTUS(山査) is known as the substance which decreases concentration of sarum lipid. The purpose of this study is to investigate the effects of CRATAEGII FRUCTUS(山査) on decreasing concentration of sarum lipid which are triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol in rat high cholesterol diet inducede hyperlipide rats. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only. Normal Group), high fat diet administered group(AIN-76 high fat diet #100496 used beef tallow for 4 weeks, Control Group) and CRATAEGII FRUCTUS(山査) administered group(AIN-76 high fat diet #100496 used beef tallow and CRATAEGII FRUCTUS(山査) extracts 85.0mg/200g 1time/day for 4 weeks. SS Group). Rats were sacrificed and concentration of triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol were measured in rat's sarum lipid Results: There was no significant difference in the level of waight between each experimental group(F=0.243, p=0.787, ANOVA test), Concentration of triglyceride has no significant difference in each group(F=0.210, p=0.813, ANOVA test). There was significant increase of total cholesterol in the Control group compared to those of Normal but there was no significant difference in the SS group compared to the Control group(F=5.237, p=0.0l9, ANOVA test). There was a significant increase of HDL-cholesterol in the SS group compared to the Control group and decrease in Control group compared Normal group(F= 11.437, p=0.001, ANDYA test). Concentration of LDL -cholesterol was significantly different in each group(F= 16.377, p=0.0001, ANDYA test). It was significantly increased in the Control group compared with the Normal group and it has no difference in the SS group compared to the control group. TBARS values(Thiobarbituric Acid Reactive Substances) were significantly different in each group(F=5.242, p=0.0019, ANDYA test). It was significantly increased in the Control group compared with the Normal group and significantly decreased in SS group compared with the Control group. Conclusions: According to the above results, it is considered that CRATAEGII FRUCTUS(山査) is effective in decreaseing concentration of sarum lipid in high cholesterol diet induced hyperlipidemic rats.

• 한국식품영양과학회지
• /
• 제22권5호
• /
• pp.511-516
• /
• 1993

간장해에 미치는 녹각추출물(water-ext., neutral ext. 및 ether-ext.)의 효과를 구명할 목적으로 녹각을 추출 분리하여 급여하고 매주 일정시각에 galactosa-mine (400mg/kg of body weight)으로 간장해를 유도한 후 녹각추출물이 간기능 상태의 지표인 효소활성에 미치는 영향을 생화학적 측면에서 비교.고찰하였다 체중증가량, 식이섭취량 및 식이효율은 Control군에 비해 galactosamine 투여군에서 감소를 보였고, 각각의 녹각추출물 급여로 증가하는 경향을 보였다. 체중100g당 장기무게는 간장의 경우 Balactosamine 투여로 현저한 증가를 보였으나 각각의 녹각추출물 급여가 이들의 수치를 감소시켰고, 신장, 비장 및 심장에는 별다른 차이가 없었다. 혈청 aminotransferase 활성은 galactosamine투여로 인해 증가하였으며, 각각의 녹각추출물중 water-ext.가 galactosamine투여에 의한 효소 활성도의 변화를 현저히 완화시켰다. 혈청 LDH와 ALP의 활성은 galactosamine투여로 증가하였고, 녹각추출물을 병행 투여한 군에서 Water-CaIN군의 활성이 Control군 수준에 가장 가깝게 감소하였다.