• The Plant Pathology Journal
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• 제35권2호
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• pp.172-177
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• 2019

A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and ${\beta}$-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was $100fg/{\mu}l$ of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.

• 한국농공학회논문집
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• 제58권2호
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• pp.45-52
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• 2016

The object of this paper was to evaulate modified proposed design equation in model test result in order to estimate uplift-resistance of timber pile of reclaimed land greenhouse foundation. Uplift resistance result of model test was increased to according to increased of contact area. Uplift-resistance result of field test tend to lineary increased to according to increased of embedment depth and contact area. Results of field uplift-resistance was evaluate compare with modified proposed design equation results of model test and Effective stress method. As the Effective stress method tend to underestimate, modified proposed design equation results of model test tend to similar type. As the contact area increase, difference between field uplift-resistance results and modified proposed design equation results of model test was considered uplift-speed.

• 한국지하수토양환경학회지:지하수토양환경
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• 제17권2호
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• pp.62-70
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• 2012

Rainfall, on Jeju Island varies regionally in relation to Mt. Halla with higher rainfall within southern area and lower in western area, and its variability is expected to expand according to the climate change scenario. Non-parametric trend analysis for rainfall, using both historic (1971-2010) and simulated (2011-2100) data assuming the A1B emissions scenario, shows regionally increasing trends with time. In perspective of agricultural land use, area for market garden including various crop types with high water demand is increasing over the Island, especially in the western area with lower rainfall compared to southern area. On the other hand, area for fruit including mandarin and kiwi with low water demand is widely distributed over southern and northern part having higher rainfall. These regional disparity of water demand/supply may be more affected by extreme events such as drought and heavy rainfall that has not yet been considered. Therefore, it is necessary to make policies for water resource management considering both demand and supply in different regions with climate change impacts over Jeju Island.

• 농촌계획
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• 제9권2호
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• pp.39-47
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• 2003

This study was carried out to find out the time-dependent change of central functions in the depopulated rural areas. Three county areas were selected for the case study ; Haenam(as a remote flat area), Goksung(as an intermediate mountainous area), and Hwasun(as a peri-urban area). For each district area administered by each county, service facilities stock was surveyed at both the present('02) and the past('89 or '94)time, and its functional index checked. From the study results, table-tennis rooms, oil shops and inns were ascertained to be disappeared now in the absolute or real terms, while beer halls, restaurants and bakeries to be sharply increased. Generally, in spite of the substantial depopulation in the past decade, service facilities stock has been increased in and concentrated to the highest order center of rural area (county office seated district). However, where this center leans to the outer side of its county area and to the opposite direction against the regional center, the dependent level of service function on the highest center have decreased.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 추계학술발표회 요지
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• pp.156-157
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 1996년도 춘계 학술대회지
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• pp.36-37
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• 1996

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.125-125
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• 2022

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.97-97
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• 2020

• 약학회지
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• 제38권6호
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• pp.725-732
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• 1994

To provide a basis for setting up an environmental pollution policy of Pusan, an investigation of metal contents in the soil of Pusan was carried out from May, 1993 to April, 1994. Soil was sampled from 10 sites of industrial area,8 sites of commercial area, 8 sites of residential area, 8 sites of green area and 8 sites of agricultural area. The industrial area was the most heavily polluted and the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.580, 19.377, 67.348, 59.638, 58.555 and 1.006 ppm, respectively. In the soil of commercial area, the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.453, 19.110, 63.384, 56.006, 37.466 and 0.578 ppm, respectively, and the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.289, 10.312, 55.246, 55.536, 17.695 and 0.610 ppm, respectively, in the soil of residential area. The green area was the least polluted and the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.215, 5.949, 19.366, 37.244, 6.856 and 0.295 ppm, respectively. In the soil of agricultural area, the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.160, 7.077, 25.365, 51.485, 10.607 and 0.499 ppm, respectively The fact that the Cd content of agricultural area was lower than that of green area is remarkable.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2001년도 학술발표회 발표논문집
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• pp.89-92
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• 2001

This study was to analyse the correlations between pumping rate to irrigated area and rainfall amount in the Geum river basins. A total of 84 pumping stations and field data from the paddy of 28,772 ha were introduced to the analysis. The results showed that the pumping volume was highly correlated to the rainfall during the irrigation period and irrigated area. But, it was difficult to determine the exact correlation factors, because of the lack of data like the efficiency of water in the paddy field.