• Title/Summary/Keyword: AGR

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Studies on Integrated Control of Citrus Pests (2) Control of ruby states (Ceroplastes rubens) on citrus by introduction of a parasitic natural enemy, Anicetus beneficus (Hymenoptera, Encyrtidae) (감귤해충의 종합적방제에 관한 연구(2) 루비붉은좀벌(Anicetus beneficus)도입에 의한 루비깍지벌레 (Ceroplastes rubens)의 방제효과)

  • Kim H.S.;Moon D.Y.;Lee S.C.;Kim H.S.;Lippold P. C.;Kim H.S.
    • Korean journal of applied entomology
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    • v.18 no.2 s.39
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    • pp.107-110
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    • 1979
  • An parasite, Anicetus beneficus was introduced from Japan to Jeju-do, Korea in 1975, and evaluated as a biological agent for control of the ruby scale, Ceroplastes rubens MASKELL. The results were as follows: 1. A major pest of citrus, ruby scale had one generation a year and hatched from late June to early August with the peak hatching in the middle of July. 2. A. beneficus was widely distributed in 16 citrus growing areas since released in 1975. 3. The pnupulation of ruby scales was considerably decreased by increasing of the parasites. Mean percent parasitism of A. beneficus was 1.7 in 1975, 14.1 in 1976, 31.0 in 1977 and 37.0 in 1978.

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Analysis of Organochlorine Pesticide, in the Presence of Polychlorinated Biphenyls(PCBs) I. Florisil Column Separation of the Pesticides-PCBs Mixture (Polychlorinated Biphenyls(PCBs) 존재하에 유기염소계 살충제의 잔류분 분석 1. Florisil Column에 의한 농약과 PCBs의 분리)

  • Park C. K.;Lee C. Y.;Park R. D.
    • Korean journal of applied entomology
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    • v.16 no.4 s.33
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    • pp.221-227
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    • 1977
  • Polychlorinated Biphenyls (PCBs) interfere with gas chromatographic analysis of multiple organochlorine pesticide residues. In the present work, existing Florisil column chromatographic method has been modified as to improve separation of organochlorine pesticides and their metabolites from PCBs. It was amply demonstrated that separation of $\alpha-BHC,\; \gamma-BHC$ Heptachlor epoxide Dieldrin, p.p-DDD, p.p'-DDT from PCBs such as Aroclor 1254 is complete and recovery of the pesticides is found quantitative. Aldrin and Heptachlor in the Aroclor eluant can be separately analyzed by comparison of the chromatographic pattern of standard Aroclor 1254 with that of Aroclor 1254 a dmixed with the two pesticides. The Florsil column technique can be utilized in the routine evaluation of the organochlorine pesticide residues by gas chromatography.

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Effect of $CaCO_3$ on the Chonggukchang Meju Fermentation by B. subtilis ($CaCO_3$가 Bacillus subtilis에 의한 청국장메주 발효에 미치는 영향)

  • Lee, Kang-Moo;Lee, Si-Kyung;Joo, Hyun-Kyu
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.421-426
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    • 1994
  • This study was carried out to investigate the effect of $CaCO_3$ on the Chungkookjang Meju fermentation. B. subtilis was cultured on the Meju added 0, 0.01, 0.1, 1% $CaCO_3$, respectively, and the chemical composition, protease activity, amino acid and vitamin B complex were examined with fermentation time. The inner temperature of the $CaCO_3$ treatments during fermentation was increased as compared with the control group. Titratable acidity in Meju decreased as $CaCO_3$ concentration increased. And protease activity and amino-nitrogen content were however high in same order. The content of amino acid was on the increase in every treatments, it made no difference between $CaCO_3$, treatments and the control. Vitamin B complex content in the $CaCO_3$ treatments was increased than in the control. The 0.01% added treatment showed the highest amount of vitamin B complex content in all the treatments.

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Growth and Histological Characteristics of Barley (Hordium vulgare L.) Seedling to NaCl Stress (NaCl Stress에 따른 보리 유묘의 생육특성 및 세포학적 반응)

  • Cho, Jin-Woong;Kim, Choong-Soo;Lee, Sok-Young;Park, Ki-Sun
    • Korean Journal of Environmental Agriculture
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    • v.17 no.4
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    • pp.335-340
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    • 1998
  • This study was conducted to determine the morphological responses of barley seedlings to NaCl stress and to investigate histological changes of cells with transmission electron microscope(TEM) after NaCl stress. Plant height and root length of 10-day old barley seedlings with NaCl stress were reduced and inhibition level was found to be more severe in the plant height than in the root length. The leaf length, leaf width and leaf area were shorter as well with NaCl stress than without NaCl stress. However, there was no difference in the number of roots between NaCl treatments. The weight of dry matter decreased at higher NaCl concentrations, especially at 100mM NaCl. The water content of shoots tend to decrease at higher NaCl concentrations, but there was no difference in the water content of roots, The reduced sugar content was greatly increase than starch. Cellulose content was higher in NaCl stressed-plant than control, and tended to decreased at higher NaCl concentrations. Lignin content also decreased NaCl stressed-plant but there was no tendency at NaCl stress concentrations. Electric conductivity of cell sap with seedlings was high with NaCl stressed-plant. Amount of cell sap gradually increased with time in the roots than in the shoots, The grana of chloroplasts was changed by 150mM NaCl concentration. The christe of mitochondria in root meristematic sells ruined in structure and cell wall of leaf and root was also ruined by NaCl stress.

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Investigation of Vegetation of University Forests at Bu-yeo (농과대학(農科大學) 부여(扶餘) 연습림(演習林)의 식생조사(植生調査))

  • Lee, Soo-wook;Kim, Chi-moon;Song, Ho-kyung
    • Korean Journal of Agricultural Science
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    • v.4 no.2
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    • pp.178-190
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    • 1977
  • The experimental forest of Cbungnam National University is located in Naryung-Ri, Eunsan-Myen, Buyeo-Gun, Chungcheongnamdo. The climax vegetations of Quercus species grown in centrual temperate zone are supposed to be destroyed before pure pine stands as a semi-climax vegetation are established on the southern aspect and along the ridges. Variability of vegetations is rather high and dominant species are such as Pinus densiflora, Quercus acutissima, Quercus variabilis, Quercus aliena, Quercus serrata, Lindera erythrocarpa, Platycarya strobilacea, and so on. There tare 203 woody plants and 128 herbal plants. According to the use of vegetations they can be classified as follows; 60 familia and 154 species for edible source, 63 familia and 143 species for medicinal, 51 familia and 140 species for forage, 32 familia and 78 species for ornamental, and 12 familia and 27 species for timber source.

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Epidemiology of Soybean Mosaic Virus Diseases (콩모자이크바이러스병의 역학적 연구)

  • Cho Eui-Kyoo;Choi S.H.;Hwang C.Y.
    • Korean journal of applied entomology
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    • v.23 no.4 s.61
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    • pp.197-202
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    • 1984
  • Two soybean cultivars Hanan and Kwanggyo were used to monitor SMV spread in the field. The Haman producing mosaic symptoms by infection with SMV strain G7 was planted in $2.2m^2$ at the coater of $320.7m^2$ field and Kwanggyo producing necrosis by infection with SMV-G7 was planted around the Haman one day after inoculation of Heman with SMV-G7. The most severe incidence of the necrotic disease in soybean cultivar Kwanggyo occurred on 43 days after planting(July 13) whereas aphid population reached peak around 22 days after planting(June 22). Total incidence of the necrotic disease was $20.4\%$, in the whole field and $56\%$, in eight $mall plots around the spreader of SMV. The spread of S MV outward from the spreader source was greater downwind than it was upwind. Spread also showed a signigicant gradieut pattern leeward from the infection focus.

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Studies on Bionomics and Control of Cutworms (거세미나방류의 생태 및 방제에 관한 연구)

  • Kim H.S.;Kim S.H.;Choi K.M.
    • Korean journal of applied entomology
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    • v.19 no.4 s.45
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    • pp.243-248
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    • 1980
  • Experiments were conducted to study on bionomics and control of cutworms; Agrotis tokionis, A. ipsilon, A. fucosa in Suweon, 1978-1979. A. ipsilon and A. fucosa had two or three generations and A. tokionis had one generation a year. A large number of A. toikonis occured at the end of September, and the major peaks of the first generation of A. tokionis and A. fucosa were in mid-June, the second generation in mid-August, and the third generation was at the end of September. These cutworms laid many eggs on the lower surface of curved downward leaf of Chinese cabbage and the larvae later than 3rd instar began to cut the basal part of stem and then pulled into the soil. A parasite of A. tokionis, a braconid wasp, Meteorus rubens, and two unidentified Ichneumnid wasps were found. Mocap and Volaton gave effective control to the cutworms but Volaton should be applied not to contact with plant.

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Induced Mutant by Gamma Rays and Genetic Analysis for Mutant Characters in Flue-cured Tobacco Variety(Nicotiana tabacum L.) II. Genetic Analysis for Mutant Characters in Flue-cured Tobacco (황색종 연초 품종의 Gamma선에 의한 돌연변이 유기 및 변이형질의 유전분석 II. 변이형질의 유전분석)

  • Jeong, Seok-Hun;Lee, S.C.;Kim, H.B.
    • Journal of the Korean Society of Tobacco Science
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    • v.14 no.2
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    • pp.116-125
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    • 1992
  • This experiment was conducted to examine characteristics of agronomic characters and estimate of gene effect for several mutant characters. The genetic populations were derived from cross between 83H-5 and Hicks. There were significant difference for plant height, stlk height, leaf shape and bacterial wilt disease index except leaf number, leaf length, and what is more, F3 variance is more than Bl and B2 generation from cross 83H-5 X Hicks. Gene actions for stalk height and bacterial wilt disease were estimated by 3-parameter, and by 6- parameter model for all characters except above two characters but stalk height and bacterial wilt disease index are not significant in the additive and dominance effects. Dominant$\times$dominant epitasis for plant height, dominant and dominant$\times$dominant epistasis for leaf length, additive and additive$\times$additive and dominant$\times$dominant epistasis for leaf width, and additive and additive$\times$dominant epistasis for days to flower were appeared significant in gene action.

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Fractionation of Iron in Rice Leaf Tissue (벼잎의 철분별 정량)

  • Park, H.;Chun, J.K.;Lee, C.Y.
    • Applied Biological Chemistry
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    • v.14 no.3
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    • pp.177-182
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    • 1971
  • A new method for the measure of iron pools using 0.02M EDTA and $Na_2S_2O_4$ was tested on Akagare diseased and healthy rice leaf tissue 1) The proposed method could fraction iron into four fractions; ferrous iron($Fe^{++}$), ferric iron($Fe^{+++}$) precipitated iron(PFe) and bound iron(BFe) well indicating the physiological status of tissue. 2) The pattern of iron pools appears to be $Fe^{+++}>PFe>BFe>Fe^{++}$ in most physiologically favorable status of iron, $PFe>Fe^{+++}>BFe>Fe^{++}$ in favorable status, $BFe>Fe^{+++}>PFe>Fe^{++}$ in unfavorable status and $BFe>PFe>Fe^{+++}>Fe^{++}$ in toxic status. 3) The percentage of each fraction to total iron was less than 10 for $Fe^{++}$, 20 to 40 for $Fe^{+++}$ and PFe and 20 to 50 for BFe. 4) Ferrous iron was always higher in upper half leaf, the appearance of which is less healthier than lower half indicating that there is more active metabolic system in which ferrous iron is involved.

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Composition and Antimicrobial Activities of Essential Oils in the Peel of Citrus Fruits

  • Oh, Hyun-Jeong;Ahn, Hyo-Min;Kim, Sang-Suk;Yun, Pil-Yong;Jeon, Gyeong-Lyong;Ko, Young-Hwan;Riu, Key-Zung
    • Journal of Applied Biological Chemistry
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    • v.50 no.3
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    • pp.148-154
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    • 2007
  • Volatile components of the essential oils of Satsuma mandarin (C. unshiu), Dangyuza (C. grandis), Yuza (C. junos), Byungkyul (C. playtymamma), Jinkyul (C. sunki), and Hakyul (C. natsudaidai) grown in Jeju Island were isolated from the fruit peels by hydro distillation and determined by GC-MS. GC-MS analysis identified 58 compounds, with main components being d-limonene $(64.01{\sim}79.34%),\;{\beta}-myrcene\;(3.01{\sim}26.53%),\;{\gamma}-terpinene\;(0.11{\sim}12.88%),\;{\beta}-pinene\;(0.78{\sim}4.74%),\;and\;{\alpha}-pinene\;(1.01{\sim}2.55%)$. Differences in compositions and contents of the essential oils were observed among citrus varieties. Effects of citrus oils on growth inhibitions of Escherchia coli, Staphyllococcus epidermidis, and Candida albicans were investigated using disc diffusion assay and minimal inhibitory concentration (MIC) assay. The essential oils inhibited growths of the test organisms, exhibiting higher levels of activity against Gram-positive S. epidermidis (MIC values $0.04{\sim}0.17mg/mL$), whereas Gram-negative E. coli was moderately resistant (MIC values $1.66{\sim}20.30mg/mL$). MIC of citrus essential oils ranged from $0.82{\sim}23.69mg/mL$ against C. albicans. The essential oils obtained from C. sunki, C. grandis, and C. playtymamma showed the highest antimicrobial activities against S. epidermidis and C. albicans, indicating their potential as natural antimicrobial agents.