• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.1-8
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• 2010

Sensitivities of PLBs of four Phalaenopsis cultivars, P. 'Taisuco Windian', P. 'Nancy Amour', P. 'Pink Twilight' and P. 'Taipei Gold' to kanamycin, spectinomycin and hygromycin at different concentrations (0, 25, 50, 100, 200, and $400mg{\cdot}L^{-1}$) were examined. Hygromycin was favorable for selecting the transformants in the genetic transformation of Phalaenopsis as PLBs of four cultivars were all dead at even $25mg{\cdot}L^{-1}$ hygromycin. Responses of PLBs of P. 'Maki Watanabe' and P. 'Brother Lawrence' to DL-phosphinothricin (PPT) were determined at different concentrations (0, 0.1. 0.25, 0.5, 1.0, 2.0, 2.5, and $5.0mg{\cdot}L^{-1}$) and $0.5mg{\cdot}L^{-1}$ PPT was thought to be suitable for selecting the transformants of Phalaenopsis. The optimum conditions for Agrobacterium cocultivation with Phalaenopsis PLBs were examined using a two-step cocultivation method in Dtps. 'City Girl' and A. tumefaciens LBA4404. In the first infection period in a 1 : 10 suspension of Agrobacterium to a VW medium, 1 hr infection showed the highest PLB survival ratio. And then, PLBs were cocultivated with a bacterial strain and a 3-day cocultivation period was better for Phalaenopsis PLBs than a prolonged period. Agrobacterium tumefaciens strains LBA4404 (pTOK233) and EHA105 (pGA643) were used to compare their efficiency on the genetic transformation of Phalaenopsis PLBs. The PLBs infected with EHA105 survived more than those infected with LBA4404 after two days in a dark condition and two weeks in light condition on a selective medium. About 1,000 PLBs for each of P. 'Maki Watanabe' and P. 'Brother Lawrence', and each bacterial strain of AGL1 (pCAMBIA3301) and LBA4404 (pTOK233) were used for the regeneration of transgenic plants. The bacterial strain AGL1 had a higher genetic transformation efficiency than LBA4404, with no significant difference between cultivars. In this study, 11 hygromycin-resistant plantlets and 32 PPT-resistant plantlets were produced, but these putative transgenic plantlets need further examinations.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.20 no.1
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• pp.39-45
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• 2012

The LSA system in Korea was introduced for the development of the aerial sporting and recreational activities. For the development of the aerial sporting and recreational activities, multi-faceted effort is needed. We discussed only for the Aeronautical Information of the aerial sporting and recreational activities. In this study, we surveyed the status of aerial sporting and recreational activities in Korea, and international AIP(Aeronautical Information Publications). We introduced the development plans for the information service of aerial sporting and recreational activities in Korea.

• Korean Journal of Plant Resources
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• v.20 no.3
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• pp.242-246
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• 2007

This study was conducted to produce the transgenic plant of rice. We obtained Agrobacterium AGL1 harbaring pCambial 300 vector with HPT gene. We carried out PCR analysis of 22 ea putative transgenic rice to investigate transformed lines. The 3 ea transgenic lines were detected insertion of HPT gene. Transgenic lines selected from PCR analysis were performed by Southern blot. From Southern blot, we obtained that two transgenic lines detected single band. We are going to study the method improving of cotransformation as well as transformation efficiency in rice.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• fall
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• pp.180-182
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• 2021

본 논문에서는 드론의 비행 장소와 온도 및 습도에 영향을 받지 않는 적외선 기반 깊이 카메라로부터 얻어진 깊이영상을 분석하여 지면 영역을 찾고 AGL(Above Ground Level) 단위의 고도를 측정하는 방법을 제안한다. Decimation filter 와 Median filter 를 적용하여 잡음 및 빈 데이터들을 제거한 깊이영상으로부터 RANSAC (RANdom Sample Consensus) 기반 평면 모델 추정 방법을 이용하여 지면 영역과 이에 대한 평면의 방정식을 유추하고 현재 위치와의 거리를 계산한다. 성능 평가를 위해 Lidar 센서와 비교한 결과, 제안 방법이 지면에 위치한 장애물에 영향을 더 적게 받으며, 자세 정보와 독립적으로 고도를 측정할 수 있었다.

• Journal of Conservation Science
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• v.37 no.3
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• pp.209-219
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• 2021

In this study, the wind environment in an urban area near Sungneymun gate was numerically investigated in the cases of inflow directions. The wind fields for the target area were simulated using Geographic Information System data and Computational Fluid Dynamics model. Results, including vector fields, three-dimensional wind velocity components, and wind speeds, were analyzed to examine flow characteristics. Wind direction variability affected by buildings was shown in the target area. The complex flows around Sungneymun did not depend on the inflow direction as a boundary condition. The wind speed around Sungneymun was generally 3 times stronger at 14 m above ground level (AGL) compared to the surface wind at 2 m AGL and relatively high in the case of easterly inflow. The effect of wind was also analyzed to be relatively significant at the southeast side of Sungneymun. Thus, it was suggested that the assessment of wind environment affected by high-rise and high-density buildings should be necessary for the architectural heritage in urban areas.

• Journal of Plant Biotechnology
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• v.50
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• pp.56-62
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• 2023

Transgenic lilies have been obtained using Agrobacterium tumefaciens (AGL1) with the plant scale explants, followed by DL-phosphinothricin (PPT) selection. In this study, scales of lily plants cv. "red flame" were transformed with the pCAMBIA3301 vector containing the gus gene as a reporter and the blpR gene as a selectable marker, as well as a gene of interest showing herbicide tolerance, both driven by the CaMV 35S promoter. Using a 20-minute infection time and a 5-day cultivation period, factors that optimized and demonstrated a high transformation efficiency were achieved. With these conditions, approximately 22-27% efficiency was observed for Agrobacterium-mediated transformation in lilies. After transformation with Agrobacterium, scales of lilies were transferred to MS medium without selective agents for 2 weeks. They were then placed on selection MS medium containing 5 mg/L PPT for a month of further selection and then cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets after transferring into hormone-free MS medium. Also, most survived putatively transformed plantlets indicated the presence of the blpR gene by PCR analysis and showed a blue color indicating expression of the gus gene. In conclusion, when 100 scales of lily cv. "red flame" are transformed with Agrobacterium, approximately 22-27 transgenic plantlets can be produced following an optimized protocol. Therefore, this protocol can contribute to the lily breeding program in the future.

• Atmosphere
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• v.21 no.4
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• pp.439-446
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• 2011

NCEP reanalysis data were analyzed in order to provide distribution of global wind resource and wind speed in the surface layer for the years 2000-2009. Wind speed at 10 m above ground level (AGL) was converted to wind speed at 80 m above the ground level using the power law. The global average 80 m wind speed shows a maximum value of $13ms^{-1}$ at the storm track region. High wind speed over the land exists in Tibet, Mongolia, Central North America, South Africa, Australia, and Argentina. Wind speed over the ocean increased with a large value in the South China Sea, Southeast Asia, East Sea of the Korea. Sea surface wind in Western Europe and Scandinavia are suitable for wind farm with a value of $7-8ms^{-1}$. Areas with great potential for wind farm are also found in Eastern and Western coastal region of North America. Sea surface wind in Southern Hemisphere shows larger values in the high latitude of South America, South Africa and Australia. The distribution of low-resolution reanalysis data represents general potential areas for wind power and can be used to provide information for high-resolution wind resource mapping.

• Transactions of the Korean Society of Automotive Engineers
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• v.24 no.3
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• pp.273-278
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• 2016

This study makes a relative comparison of the results of tensile test and quasi-static analysis using AGL(Adjuster Guide Loop) model that plays a role in adjusting the height of shoulder belt, of the components of the vehicle seatbelt system and attempts to propose a method of reducing the error rate of the quasi-static analysis technique effectively. This study selects two major factors affecting the result of an analysis, draws the result of analysis through the method of experimental design, one of the statistical techniques and understands the contribution rate of the major factors affecting the result of the analysis through ANOVA(Analysis of Variance).

• Journal of the Korean Society for Aviation and Aeronautics
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• v.29 no.2
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• pp.94-99
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• 2021

UTM is an air traffic management ecosystem under development for autonomously controlled operations of UAS by the FAA, NASA, other federal partner agencies, and industry. They are collaboratively exploring concepts of operation, data exchange requirements, and a supporting framework to enable multiple UAS operations beyond visual line-of-sight at altitudes under AGL 500ft in airspace where air traffic services are not provided. Minimum Safe Altitude is a generic expression, used in various cases to denote an altitude below which it is unsafe to fly owing to presence of terrain or obstacles. The European drone regulation mentions that the UAS is maintained within 120 metres from the closest point of the surface of the earth during flight, except when overflying an obstacle. This study attempted to develop a minimum flight altitude database system. Based on domestic and international rules and regulations on setting the minimum flight altitude it is expected that it can be applied to the operation of aircraft and unmanned aerial system in UTM environments for specific area in Korea.

• Wind and Structures
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• v.35 no.6
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• pp.385-394
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• 2022

The energy demand of the world is increasing rapidly, mainly using fossil energy, which causes environmental damage. The wind is free and clean energy to solve the environmental problems. Thailand is one of the developing nations, and the majority of its energy is obtained from petroleum, natural gas and coal. The objective of this study is to test the characteristics of wind energy at Khon Kaen in Thailand. The wind measurement tools, the 3-cup anemometers to measure wind speed, and wind vanes to measure wind direction, were mounted on a wind tower mast to record wind data at the heights of 60, 90 and 120 meters above ground level (AGL) for 5 years between January 2012 and December 2016. The results show that the annual mean wind speeds were 3.79, 4.32 and 4.66 m/s, respectively. The highest mean wind speeds occurred in June, August and December, in order, and the lowest occurred in September. The majority of prevailing wind directions were from the North-East and South-West directions. The average annual wind shear coefficient was 0.297. Furthermore, five wind turbines with rated power from 0.85 to 4.5 MW were selected to estimate the wind energy output and it was found that the maximum AEP and CF were achieved from the low cut-in speed and high hub-height wind turbines. This important information will help to develop wind energy applications, such as the plan to produce electricity and the calculation of the wind load that affects tall and large structures.