• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1529-1539
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• 2017

Objective: The objective of this study was to compare the DNA methylation profile in the longissimus dorsi muscle between Small Tailed Han and Dorper${\times}$Small Tailed Han crossbred sheep which were known to exhibit significant difference in meat-production. Methods: Six samples (three in each group) were subjected to the methylated DNA immunoprecipitation sequencing (MeDIP-seq) and subsequent bioinformatics analyses to detect differentially methylated regions (DMRs) between the two groups. Results: 23.08 Gb clean data from six samples were generated and 808 DMRs were identified in gene body or their neighboring up/downstream regions. Compared with Small Tailed Han sheep, we observed a tendency toward a global loss of DNA methylation in these DMRs in the crossbred group. Gene ontology enrichment analysis found several gene sets which were hypomethylated in gene-body region, including nucleoside binding, motor activity, phospholipid binding and cell junction. Numerous genes were found to be differentially methylated between the two groups with several genes significantly differentially methylated, including transforming growth factor beta 3 (TGFB3), acyl-CoA synthetase long chain family member 1 (ACSL1), ryanodine receptor 1 (RYR1), acyl-CoA oxidase 2 (ACOX2), peroxisome proliferator activated receptor-gamma2 (PPARG2), netrin 1 (NTN1), ras and rab interactor 2 (RIN2), microtubule associated protein RP/EB family member 1 (MAPRE1), ADAM metallopeptidase with thrombospondin type 1 motif 2 (ADAMTS2), myomesin 1 (MYOM1), zinc finger, DHHC type containing 13 (ZDHHC13), and SH3 and PX domains 2B (SH3PXD2B). The real-time quantitative polymerase chain reaction validation showed that the 12 genes are differentially expressed between the two groups. Conclusion: In the current study, a tendency to a global loss of DNA methylation in these DMRs in the crossbred group was found. Twelve genes, TGFB3, ACSL1, RYR1, ACOX2, PPARG2, NTN1, RIN2, MAPRE1, ADAMTS2, MYOM1, ZDHHC13, and SH3PXD2B, were found to be differentially methylated between the two groups by gene ontology enrichment analysis. There are differences in the expression of 12 genes, of which ACSL1, RIN2, and ADAMTS2 have a negative correlation with methylation levels and the data suggest that DNA methylation levels in DMRs of the 3 genes may have an influence on the expression. These results will serve as a valuable resource for DNA methylation investigations on screening candidate genes which might be related to meat production in sheep.

• Proceedings of the KIEE Conference
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• 2002.07b
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• pp.1097-1099
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• 2002

본 논문에서는 제어용 전원으로 사용할수 있는 주파수 제어 직렬 공진형 컨버터의 동작 원리 및 특성을 살펴보고 실제의 제어용 전원을 설계 및 제작하려고 한다. 고성능의 SMPS 제작을 위한 공진형 컨버터 회로를 구성하고 실제 시스템 제작에 앞서 먼저 시뮬레이션을 통한 시스템의 분석을 하였다. 시뮬레이션 프로그램은 ACSL(Advanced Continuous Simulation Language)을 사용하였다.

• Proceedings of the KIPE Conference
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• 2003.07a
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• pp.69-72
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• 2003

In this paper new control strategy of series resonant converter system for control power supply is suggested. Frequency controlled series resonant converter system is robust to load variations because it is POSR(parallel output series resonant) type. And it provides stable output voltage by changing switching frequency to input voltage variations. Firstly, operation analysis about suggested series resonant converter system was carried. Then simulations using ACSL(Advanced Continuous Simulation Language) and experiments to actual system were carried to prove characteristics of suggested system.

• Proceedings of the KIEE Conference
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• 2001.04a
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• pp.473-475
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• 2001

This paper proposed a MPPT ( Maximum Power Point Tracking) control algorithm for PV(Photovoltaic) array based on a modified constant voltage control MPPT algorithm at low-insolation. This method which combines a IncCond(Incremental Conductance) and a constant voltage control algorithm. In contrast to the typical conventional MPPT algorithm, the proposed method have been obtained high efficiency and good performance. The proposed algorithm is verified through simulation result. In order to confirm the availability of the scheme, a simulation used PSIM and ACSL software tool.

• Proceedings of the KSR Conference
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• 2002.10a
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• pp.440-445
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• 2002

In this paper, traction system for urban transit maglev system is proposed. Using vector control strategy to control magnitude and frequency of output voltage transiently is general. But in case of traction system for railway vehicle, it is impossible that adapt vector control because there is one-pulse mode in a high speed region. So this paper proposes the control strategy using vector control in a low speed region and slip frequency control in a high speed region. And also proposes overmodulation method that makes to change in one-pulse mode softly. The performance of traction system will be verified by simulation results using ACSL.

• Proceedings of the KAIS Fall Conference
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• 2003.06a
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• pp.271-274
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• 2003

본 논문에서는 통신 기지국용 전원으로 사용할 수 있는 주파수 제어 직렬 공진형 컨버터의 동작 원리 및 특성을 살펴보고 실제의 전원 시스템을 설계 및 제작하려고 한다. 주파수 제어 공진형 컨버터 시스템은 POSR(Parallel Output Series Resonant) 형태이기 때문에 부하에 강인하고 주파수를 제어함으로써 입력전압의 변화에도 안정된 출력을 보여준다. 실제 시스템 제작에 앞서 먼저 시뮬레이션을 통한 시스템의 분석을 하였고 시뮬레이션 프로그램은 ACSL(Advanced Continuous Simulation Language)을 사용하였다.

• Nutrition Research and Practice
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• v.10 no.5
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• pp.477-486
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• 2016

BACKGROUND/OBJECTIVES: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD. MATERIALS/METHODS: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR. RESULTS: In intestine, significantly lower Cd36 mRNA expression (P<0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P<0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly. CONCLUSIONS: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

• The Transactions of the Korean Institute of Power Electronics
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• v.5 no.6
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• pp.584-591
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• 2000

Power Quality Compensator(PQC) has been installed to protect the sensitive loads against the voltage disturbances, such as voltage sag and interruption. In general, static switch is used for the purpose of link between utility and PQC. So transfer operation of the static switch play a important part in the PQC. Many studies on the structure and control of PQC have been progressed in active, but these researches have been rarely mentioned about any voltage-disturbances-detection method to start the PQC operation. In this paper, a new voltage-disturbances-detection algorithm for computer application loads using the CBEMA/ITIC curve is proposed for transfer operation of the static switch. The proposed detection algorithm is implemented to get fast detecting time through the comparison of instantaneous 3-phase voltage values transferred to DC values in the synchronous reference frame with the operating reference values. To get the robust characteristics against the noise, a first order digital filter is designed. The magnitude falling and phase delay caused by the filter are compensated through the error normalizing and numerical analysis using transfer function, respectively. Finally, the validity of the proposed algorithm is proved by ACSL simulation and experimental results.

• Journal of Animal Science and Technology
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• v.64 no.6
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• pp.1199-1214
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• 2022

Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway. This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction.

• Proceedings of the KIPE Conference
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• 1997.07a
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• pp.200-203
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• 1997

This paper describes an study electric injection system for diesel engines. It is needed effective fuel injection which controls the solenoid valve of fuel pump. To solve this, this paper proposes DCC-PWM method which can realize fast reply and low holding current for solenoid valve on/off. For the proposed design method, simulation tools of ACSL are used to analyze the system. And the single-chip microcomputer is used to reduce the size of controller and to improve flexibility. And the system's validity can be verified through the experimental results.