• Title/Summary/Keyword: ACE inhibition activity

Search Result 182, Processing Time 0.032 seconds

Angiotensin Converting Enzyme Inhibitory Activity of Skipjack/Yellow Tuna Cooking Broth (참치 자숙액의 Angiotensin 전환효소 저해작용)

  • Yeo, Saeng-Gyu;Lee, Tae-Gee;Ahn, Cheol-Woo;Kim, In-Soo;Gu, Yeun-Suk;Park, Yeong-Ho;Kim, Seon-Bong
    • Journal of Life Science
    • /
    • v.8 no.3
    • /
    • pp.312-317
    • /
    • 1998
  • This study was designed to investigate the angiotensin convertin enzyme (ACE) inhibitory activity of skipjack/yellowpin tuna cooking broth. The cooking broth was pretreated with membrane filter (MW cut-off 5,000) to obtain the peptide fraction with ACE inhibition. the crude peptides fractionated with Amberlite IR-120 ($H^{+}$ form and followed by Bio-gel P-2, were separated into nine fractions (T-1 to T-9). The maximum inhibitory activity was observed in the fraction T-4 ($IC_{50}$ value, 0.619mg/ml). The abundant amino acids obtained from active fraction T-4 were phenylaanine, leucine and glutamic acid.

  • PDF

Optimization of Whey-Based Medium for Growth and ACE-Inhibitory Activity of Lactobacillus brevis

  • Ahn, Jae-Eun;Park, Seung-Yong;Lee, Byong-H.
    • Journal of Dairy Science and Biotechnology
    • /
    • v.25 no.1
    • /
    • pp.1-7
    • /
    • 2007
  • A Whey-based medium was formulated with Lactobacillus brevis to investigate whether any functional peptides could derive from whey protein. The optimal concentrations of the ingredients of the medium for the growth of Lactobacillus were determined as 2% whey protein concentrate and 1% glucose and 0.5% yeast extracts. The growth of Lb. brevis was improved with the supplementation of yeast extracts than glucose. The viable cells counts of Lb. brevis reached to 2.0 × 10$^8$CFU/mL in the whey-based medium. The whey protein hydrolysates recovered from the supernatant after centrifugation at 10,000 x g for 10min induced strong inhibitory activity against ACE. When the whey protein hydrolysate were partially purified by a membrane tubing below 8,000Da, the partially purified fraction remained 64.7 ${\pm}$ 3.6% of the ACE inhibition activity of the whey protein hydrolysates and IC$_{50}$ was 38.8 ${\pm}$ 2.2mg/mL. The whey-based medium was proved to be effective in producing ACE inhibitory peptides by lactic bacteria fermented whey protein.

  • PDF

Studies on the Radical Scavenging Effects and the Inhibitory Effects on ACE Activity of Several Flavonoids (각종 Flavonoids의 라디칼 봉쇄능과 ACE 활성 억제능에 관한 연구)

  • 강진훈
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.32 no.8
    • /
    • pp.1318-1322
    • /
    • 2003
  • This study was carried out to identify the biophysical utility of bioflavonoids by the determination of their antioxidative activities, radical scavenging activity and inhibitory effect on the ACE activity. The results obtained were as follows; All flavonoids experimented greatly inhibited the linoleic acid oxidation from the early period of oxidation, and the radical scavenging ability was also greater in genistein and daidzein than other flavonoids, generally showing donating ability. Rutin has the metal-chelating ability with C $u^{2+}$ and $Mg^{2+}$, which means to have the inhibitory effect on the promotive oxidation of lipid by metal ion. All flavonoids experimented inhibited the angiotensin converting enzyme (ACE) activity, which was greater in genistein and daidzein than other flavonoids.s.

Immunomodulatory Effects of Euglena gracilis Extracts (Euglena gracilis 추출물의 면역조절 및 생리활성 분석)

  • Yu, Sun Nyoung;Park, Bo Bae;Kim, Ji Won;Hwang, You Lim;Kim, Sang Hun;Kim, Sunah;Lee, Taeho;Ahn, Soon Cheol
    • Journal of Life Science
    • /
    • v.31 no.2
    • /
    • pp.183-191
    • /
    • 2021
  • Euglena gracilis is a microalga of great biotechnological interest that can create high levels of bioactive compounds, such as tocopherol, paramylon, and folic acid. The objective of this study was to investigate the biological activities of extracts from E. gracilis, especially those focused on immunological activity. E. gracilis biomass was extracted with hot water (HWE) and the remaining pellet was continuously extracted with methanol (HWME). First, we examined the effect of two extracts from E. gracilis on the production of nitric oxide (NO) and the expression of pro-inflammation cytokines, including IL-1β, IL-6, and TNF-α in murine macrophage RAW 264.7 cells. HWE treatment dose-dependently increased the production of IL-1β and TNF-α. On the other hand, treatment with HWME significantly decreased the generation of NO and pro-inflammatory cytokines (IL-6 and TNF-α) in lipopolysaccharide (LPS)-stimulated macrophage cells. In addition, other biological activities of the extracts were further analyzed: α-glucosidase inhibition, protein tyrosine phosphatase (PTP1B) inhibition, tyrosinase inhibition, xanthine oxidase (XO) inhibition, and angiotensin-converting enzyme (ACE) inhibition. Analysis of these biological activities showed that HWE has more inhibitory effects than HWME against α-glucosidase, tyrosinase, and XO agents. However, the inhibition of PTP1B and ACE with HWME were higher than with HWE. Taken together, the results suggested that E. gracilis possesses various biological activities―especially immunological capabilities―through regulation of cytokine production. Therefore, E. gracilis extract may be potentially useful for food material with immune-regulating effects.

Isolation of Angiotensin Converting enzyme inhibitors from Ripe Cucurbita moschata Duch

  • Hyeyoung Jung;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
    • /
    • v.6 no.4
    • /
    • pp.244-246
    • /
    • 2001
  • Angiotensin converting enzyme (ACE) inhibitor acts on the inhibition of ACE and causes a decrease in blood pressure. There have been several reports on screening of ACE inhibitors from natural food products and protein hydrolysates of various food sources. Ripe Cucurbita moschata Duch has been used as an oriental medicine in Korea. To isolate ACE inhibitors, crude water extracts of the edible portion of ripe Cucurbita moschata Duch were obtained after heating in water at 95$^{\circ}C$ for 2 h. Crude extracts were then filtered using PM-10 and YM-1 membranes. The membrane-filtered solution was loaded onto Sephadex G-15 column equlibrated with a phosphate buffer. Among the four major fractions of gel permeation chromatography, the second fraction had the highest inhibitory activity of 65%. Further purification of the fraction using reversed-phase HPLC with a $C_{18}$ column produced ACE inhibitors, which were identified as a mixture having molecular mass of 222 and 273 by Tandem mass spectrometry.

  • PDF

Effect on Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activities of Soybean (Glycine max L.) following Steaming and Drying Nine Times (구증구포 처리 대두 추출물의 항산화 활성 및 Angiotensin-I Converting Enzyme 저해 효과)

  • Kim, Hyun Young;Seo, Hye-Young;Seo, Woo Duck;Lee, Mi Ja;Choi, Man-Soo;Ham, Hyeonmi
    • The Korean Journal of Food And Nutrition
    • /
    • v.33 no.2
    • /
    • pp.167-173
    • /
    • 2020
  • To increase the functional material content of soybean, a repetitive steaming and drying process was used. We investigated the changes in the total polyphenol content, the antioxidant activity, and the angiotensin-I converting enzyme (ACE) inhibition in soybean following nine rounds of steaming and drying. Soybean was steamed 9 times for 2 h and then dried 9 times from 55℃ to 73℃ for 3 h. The total polyphenol content in the soybean reached a maximum value of 60.47 mg GAE eq./100 g at 73℃ while the total polyphenol content in the raw soybean reached 25.17 mg GAE eq./100 g. In the raw soybean samples, the DPPH radical scavenging activity (5 mg/mL) was 8.04% but it increased by 43.29% after drying 9 times to 73℃. ABTS radical scavenging activity also improved following 9 rounds of steaming and drying. ACE inhibitory activity of the soybean dried 9 times at 73℃ was 58.94% at 10 mg/mL. These results showed that steaming and drying soybean 9 times enhanced the antioxidant activity and the ACE inhibitory activity of soybean. Therefore, more research on the biological and anti-hypertensive activity of soybean using this steaming and drying method is necessary.

Functional Properties of Nutmeg (육두구의 생리활성에 관한 연구)

  • Bok, Jin-Heuing;Takeda, Yasutuki;Ando, Kouichi;Son, Jong-Youn
    • Korean journal of food and cookery science
    • /
    • v.23 no.1 s.97
    • /
    • pp.33-40
    • /
    • 2007
  • This study investigated the antioxidative and antimicrobial activities of nutmeg (water, ethanol extract and essential oil). The total phenol contents of water, ethanol extract and essential oil were 3.4%, 16.9%, and 3.2%, respectively. Hydrogen donating abilities of water, ethanol extract and essential oil at 1,000 ppm were 4.9%, 41.8% and 6.8%, respectively. The antioxidative activities in linoleic acid substrates were in the order of BHT > ethanol > extract > ${\alpha}$-tocopherol > essential oil > water extract. The antioxidative activities in linoleic acid emulsion substrates were in the order of BHT > water extract > essential oil > ethanol extract > ${\alpha}$-tocopherol. In antimicrobial activity, ethanol extract showed growth inhibition effect against Micrococcus luteus, Bacillus cereus and Salmonella enteritidis, and the essential oil showed growth inhibition effect against Micrococcus luteus. However, no antimicrobial activity of water extract was observed. The nitrite-scavenging abilities of water, ethanol extract and essential oil at 1,000 ppm were 4.5%, 28.8% and 98.8%, respectively, and the ACE inhibitory activities were 0.2%, 11.0% and 10.0%, respectively.

Inhibitory Effect against Angiotensin Converting Enzyme and Antioxidant Activity of Panax ginseng C. A. Meyer Extracts (인삼 추출물의 Angiotensin Converting Enzyme 저해 효과와 항산화 활성)

  • Lee, Seung-Eun;Seong, Nak-Sul;Bang, Jin-Ki;Kang, Seung-Won;Lee, Sung-Woo;Chung, Tae-Yung
    • Korean Journal of Medicinal Crop Science
    • /
    • v.11 no.3
    • /
    • pp.236-245
    • /
    • 2003
  • The study was performed for elucidating angiotensin converting enzyme (ACE) inhibitory activity and comparing antioxidative activity of Panax ginseng extracts prepared at different conditions. Total phenolic content, inhibitory activity on ACE and antioxidative effects were tested on 10 ethanolic extracts and correlation coefficient between total phenolic content and physiological activity was calculated. Yield and total phenolic content of 50% ethanolic extract prepared at $85^{\circ}C$ exhibited the highest value as 42.52% and 0.82%, respectively. Among the fractions obtained from 50% ethanolic extract prepared at room temperature, water fraction showed the highest value in yield as 72.08% and ethyl acetate fraction did in total phenolic content as 6.59%. In the test on ACE inhibitory activity, 50% ethanolic extract obtained at room temperature indicated the strongest effect of 93.8% which was higher than 85.2% of commercialized ACE inhibitor and solvent fractions showed potent inhibitory activity in order of hexane fraction, diethyl ether fraction, ethyl acetate fraction, butanol fraction and water fraction at concentration of $4000{\mu}g/ml$. 50% Ethanolic extract prepared at $85^{\circ}C$ had the most potent inhibition effect on human LDL oxidation as 78.2% at $200{\mu}g/ml$ and the other extracts also did above 60%. Diethyl ether fraction and ethyl acetate fraction showed strong inhibition activity $(34.38%{\sim}78.13%)$ on LDL oxidation at concentration of $10{\sim}200\;{\mu}g/ml$. From the statistical analysis via SAS program, correlation coefficient between total phenolic content and ACE inhibitory effect was 0.6353 at P<0.05. Conclusively, this report showed that the most efficient extraction condition for elevating inhibitory activity on ACE and LDL oxidation, phenolic content and yield from Panax ginseng was 50% ethanol extraction at room temperature or high temperature condition. And Panax ginseng would be used for preventing hypertension or atheroscrelosis for man via inhibitory action on ACE and LDL oxidation.

Screening of Biological Activity for Phenolic Fraction from Onion (양파 페놀성분획물의 생리활성기능탐색)

  • 안봉전;이진태
    • Food Science and Preservation
    • /
    • v.8 no.2
    • /
    • pp.224-230
    • /
    • 2001
  • To develop a material for functional food, the fraction Ⅰ,II and Ⅲ of onion extract were separated by Sephadex LH-20 column. The revealed purification levels of each fraction were 5.2%-6.5%, 12.2%-14.5% and 82.6%-86.7%, respectively. A functional activity test was performed with fraction Ⅲ. The ACE inhibition activity was about 50% at 5mg and about 80% at 10mg. The XOase inhibition activity of Korean Changnyong Daego was 84%, which was the highest. At the experiment of tyrosinase inhibition was detected 21-36% at 10mg. The cholesterol dose in mice blood was as low as low as 79$\pm$5(mg/100 mL) after the intake of fraction Ⅲ. Cholestrol dose in liver was decreased to below 50%. The results show that Sephadex LH-20 column method was very useful as a fraction method for the development of functional food material nsing onions.

  • PDF

Studies on the Physiological Functionality of Pine Needle and Mugwort Extracts (솔잎과 쑥 추출물의 기능성 점토)

  • Kang, Yoon-Han;Park, Yong-Kon;Oh, Sang-Ryong;Moon, Kwang-Deog
    • Korean Journal of Food Science and Technology
    • /
    • v.27 no.6
    • /
    • pp.978-984
    • /
    • 1995
  • This study was conducted to investigate the physiological functionality of the hot water and 70% acetone extracts obtained from the pine needle and mugwort. Flavanol tannin content was above 60% of the total polyphenol in pine needle extracts. 70% Acetone extract from pine needle exhibited inhibition percentage of about 82.2% on the mutagenicity of Trp-P-1. Angiotensin I converting enzyme(ACE) inhibition activity was 61% and 50% in the hot water extract and in the 70% acetone extract respectively. Electron donating ability(EDA) of the hot water and 70% acetone extracts obtained from the pine needle was significantly good above 80%. 70% Acetone extract from pine needle showed inhibitary effect against the polyphenol oxidase(PPO) of water dropwort. The nitrite scavenging ability was appeared in all the extracts examined and it showed $80{\sim}90%$ at pH 3.0. The oxidative stability was determined by POV. Results showed that ethyl acetate fraction is better antioxidants than chloroform and butanol fractions in the soybean oil. Then, in the mugwort extracts, antimutagenicity of 70% acetone extract was similar to that of pine needle. Ratio of ACE inhibition activity was higher than that of pine needle extracts. EDA of hot water and 70% acetone extracts was 45%. Extracts from the mugwort were lower in nitrite scavenging ability about $20{\sim}30%$ than extracts from pine needle.

  • PDF