Inhibitory Effect against Angiotensin Converting Enzyme and Antioxidant Activity of Panax ginseng C. A. Meyer Extracts

인삼 추출물의 Angiotensin Converting Enzyme 저해 효과와 항산화 활성

  • Published : 2003.09.30

Abstract

The study was performed for elucidating angiotensin converting enzyme (ACE) inhibitory activity and comparing antioxidative activity of Panax ginseng extracts prepared at different conditions. Total phenolic content, inhibitory activity on ACE and antioxidative effects were tested on 10 ethanolic extracts and correlation coefficient between total phenolic content and physiological activity was calculated. Yield and total phenolic content of 50% ethanolic extract prepared at $85^{\circ}C$ exhibited the highest value as 42.52% and 0.82%, respectively. Among the fractions obtained from 50% ethanolic extract prepared at room temperature, water fraction showed the highest value in yield as 72.08% and ethyl acetate fraction did in total phenolic content as 6.59%. In the test on ACE inhibitory activity, 50% ethanolic extract obtained at room temperature indicated the strongest effect of 93.8% which was higher than 85.2% of commercialized ACE inhibitor and solvent fractions showed potent inhibitory activity in order of hexane fraction, diethyl ether fraction, ethyl acetate fraction, butanol fraction and water fraction at concentration of $4000{\mu}g/ml$. 50% Ethanolic extract prepared at $85^{\circ}C$ had the most potent inhibition effect on human LDL oxidation as 78.2% at $200{\mu}g/ml$ and the other extracts also did above 60%. Diethyl ether fraction and ethyl acetate fraction showed strong inhibition activity $(34.38%{\sim}78.13%)$ on LDL oxidation at concentration of $10{\sim}200\;{\mu}g/ml$. From the statistical analysis via SAS program, correlation coefficient between total phenolic content and ACE inhibitory effect was 0.6353 at P<0.05. Conclusively, this report showed that the most efficient extraction condition for elevating inhibitory activity on ACE and LDL oxidation, phenolic content and yield from Panax ginseng was 50% ethanol extraction at room temperature or high temperature condition. And Panax ginseng would be used for preventing hypertension or atheroscrelosis for man via inhibitory action on ACE and LDL oxidation.

한국산 백삼으로부터 온도 및 에탄올 함유 비율을 달리하여 조제된 추출물들에 대한 angiotensin converting enzyme (ACE) 저해활성을 검정하고 항산화 활성을 비교하기 위해 본 연구를 수행하였으며 그 결과는 다음과 같다. 1. 10가지 조건에서 얻어진 추출물의 인삼분말에 대한 수율과 총 페놀함량은 $85^{\circ}C$에서 50% 에탄올로 추출했을 때 42.25% 및 0.82%로 가장 높았으며 대체적으로 가온조건이 상온조건보다 높았다. 상온 50% 에탄을 추출물로부터 조제된 분획물의 수율은 물 분획이 72.08%로 가장 높았고, 총 페놀함량은 에틸아세테이트 분획이 6.59%로 가장 높았다. 2. 10가지 추출물의 ACE 저해활성을 실험한 결과 $4000{\mu}g/ml$의 농도에서 상온 50% 에탄올 추출물이 93.8%로 가장 우수한 효과를 보였으며 이 값은 시판 ACE inhibitor의 85.2%보다도 월등하게 높은 수치였으며 분획물은 핵산(201%) > 에텔 (105%) > 에틸아세테이트 (67%) > 부탄올 (46%) < 물 분획 (19%)의 순으로 효과가 높았다. 3. 사람 LDL에 대한 산화저해효과 실험에서는 $85^{\circ}C$에서 얻어진 50% 에탄올 추출물이 $200{\mu}g/ml$의 농도에서 78.2%로 가장 효과적으로 항산화력을 보였으며 모든 추출물들이 60%이상의 높은 산화저해효과를 나타내었다. 분획물에 대한 LDL산화저해효과 검정 실험에서는 에텔 분획물 및 에틸아세테이트 분획물이 $10{\sim}200\;{\mu}g/ml$의 농도에서 $34.38%{\sim}78.13%$로 높은 활성을 보였다. 4. 각 에탄올 추출물의 linoleic acid 과산화저해 및 DPPH라디칼 소거효과는 그다지 높지 않았으며 비교 실험된 시판 천연 항산화제인 ${\alpha}-tocopherol$보다 매우 낮았다. 5 상관분석의 결과 총 페놀함량과 ACE 저해활성은 P<0.05의 수준에서 0.6353의 유의적인 상관성을 나타내었다. 이상의 실험결과로부터 인삼이 ACE 저해 및 LDL산화 저해작용을 효과적으로 나타내고 총 페놀함량이 많이 추출될 수 있으며 수율을 높일 수 있는 가장 적합한 조건은 상온 혹은 가온의 50% 에탄올 추출조건이었으며 인삼은 이러한 활성에 의해 항고혈압 및 동맥경화예방의 효과를 발휘 할 수 있을 것으로 기대되었다.

Keywords

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