• 한국식품과학회지
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• 제25권5호
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• pp.456-460
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• 1993

전통 기호음료 성분에 의하여 나타나는 기능특성을 조사하기 위한 연구의 일환으로 결명자,들깨, 대추, 모과, 오미자, 오갈피 및 생강 추출물을 ion-exchange chromatography, 유기용매에 의한 분획, silica gel column chromatography, thin layer chromatography에 의하여 여러 가지 획분으로 분획하여 Angiotensin I 전환효소(ACE) 저해작용을 조사한 결과는 다음과 같다. 기호음료 원료에서 추출한 수용성 획분의 ACE 저해작용은 생강>오갈피>오미자>들깨>결명자>모과>대추의 순으로 나타났다. 일반 가정에서 널리 이용되고 있는 결명자에서 분리한 compound C 는 ACE 저해제로 알려져 있는 bradykinin에 비하여 ACE 저해작용이 비교적 낮았다.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2005년도 제36차 추계 학술발표대회
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• pp.329-333
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• 2005

ACE inhibition activity of peptides was measured after 2 months of storage at $4^{\circ}C$ under condition of pH 6.0, 6.5, 7.0, 7.5, 8.0. and the ACE inhibitory activity were changed only slightly. After 2 months of chilled storage ($4^{\circ}C$), no dramatic change and significance was found. This indicates that acidic, neutral, weak alkali conditions did not affect ACE inhibitory activity of those peptides. Among peptide 1134, 1152, and 1155, peptides from thermolysin + protease A hydrolysates, inhibition activity of peptide 1134 and 1152 was decreased significantly at $60^{\circ}C$, however, they showed stable inhibition activity from $70^{\circ}C$ to $100^{\circ}C$ (P<0.001). Also, chromatogram of peptide 1134, 1152, and 1155 was shown that retention time of peptide of $60^{\circ}C$ was not correspond to the retention time of the rest of peptides. This indicated that temperature may change the inhibitory activity and profile of peptides.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.741-746
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• 2005

To investigate the basic information and the possibility of ACE-inhibitory peptides for antihypertension materials, goat's caisin (CN) was hydrolyzed by various proteolytic enzymes and ACE-inhibitory peptides were separated and purified. ACE-inhibition ratios of enzymatic hydrolysates of goat's CN and various characteristics of ACE-inhibitory peptides were determined. ACE-inhibition ratios of goat's CN hydrolysates were shown the highest with 87.84% by pepsin for 48 h. By Sephadex G-25 gel chromatograms, Fraction 3 from goat's CN hydrolysates by pepsin for 48 h was confirmed the highest ACE-inhibition activity. Fraction 3 g and Fraction 3 gh from peptic hydrolysates by RP-HPLC to first and second purification were the highest in ACE-inhibition activity, respectively. The most abundant amino acid was leucine (18.83%) in Fraction 3 gh of ACE-inhibitory peptides after second purification. Amino acid sequence analysis of Fraction 3 gh of ACE-inhibitory peptides was shown that the Ala-Tyr-Phe-Tyr, Pro-Tyr-Tyr and Tyr-Leu. IC$_{50}$ calibrated in peptic hydrolysates at 48 h, Fraction 3, Fraction 3 g and Fraction 3 gh from goat's CN hydrolysates by pepsin for 48 h were 29.89, 3.07, 1.85 and 0.87 g/ml, respectively. Based on the results of this experiment, goat's CN hydrolysates by pepsin were shown to have ACE-inhibitory activity.

• BMB Reports
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• 제31권5호
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• pp.459-467
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• 1998

A new set of functional group interaction energy descriptors relevant to the ACE (Angiotensin-Converting Enzyme) inhibitory peptide, QSAR (Quantitative Structure Activity Relationships), is presented. The functional group interaction energies approximate the charged interactions and distances between functional groups in molecules. The effective energies of the computationally derived geometries are useful parameters for deriving 3D-QSAR models, especially in the absence of experimentally known active site conformation. ACE is a regulatory zinc protease in the renin-angiotensin system. Therapeutic inhibition of this enzyme has proven to be a very effective treatment for the management of hypertension. The non bond interaction energy values among functional groups of six-feature of ACE inhibitory peptides were used as descriptor terms and analyzed for multivariate correlation with ACE inhibition activity. The functional group interaction energy descriptors used in the regression analysis were obtained by a series of inhibitor structures derived from molecular mechanics and semi-empirical calculations. The descriptors calculated using electrostatic and steric fields from the precisely defined functional group were sufficient to explain the biological activity of inhibitor. Application of the descriptors to the inhibition of ACE indicates that the derived QSAR has good predicting ability and provides insight into the mechanism of enzyme inhibition. The method, functional group interaction energy analysis, is expected to be applicable to predict enzyme inhibitory activity of the rationally designed inhibitors.

• Nutrition Research and Practice
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• 제5권2호
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• pp.93-100
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• 2011

Inhibition of angiotensin I-converting enzyme (ACE) activity is the most common mechanism underlying the lowering of blood pressure. In the present study, five organic extracts of a marine brown seaweed Ecklonia cava were prepared by using ethanol, ethyl acetate, chloroform, hexane, and diethyl ether as solvents, which were then tested for their potential ACE inhibitory activities. Ethanol extract showed the strongest ACE inhibitory activity with an $IC_{50}$ value of 0.96 mg/ml. Five kinds of phlorotannins, phloroglucinol, triphlorethol-A, eckol, dieckol, and eckstolonol, were isolated from ethanol extract of E. cava, which exhibited potential ACE inhibition. Dieckol was the most potent ACE inhibitor and was found to be a non-competitive inhibitor against ACE according to Lineweaver-Burk plots. Dieckol had an inducible effect on the production of NO in EAhy926 cells without having cytotoxic effect. The results of this study indicate that E. cava could be a potential source of phlorotalnnins with ACE inhibitory activity for utilization in production of functional foods.

• Applied Biological Chemistry
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• 제51권2호
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• pp.93-101
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• 2008

본 연구는 내산성과 담즙산 내성이 뛰어나고 건강증진 효과가 우사한 L. acidophilus를 신생아의 분변에서 분리 및 선발하고 이들에 의한 장내 유해미생물 생육억제, 혈중 cholesterol 저하 및 과산화수소에 대한 저항성과 탈지유 발효를 통한 풍미성분 형성, ACE 저해활성, 철분가용성 증진 등에 관한 기초 지식을 확보하여 다양한 생리활성 기능을 가진 유산균을 개발하고자 실시하였다. 실험 결과 신생아(생후 2주 이내)에서 분리된 5종의 L. acidophilus 중 특히 L. acidophilus SD 105는 Listeria sp.에 대한 항균활성, 복합담증산염 분해활성 및 항산화효과가 다른 시험 균주에 비해 모두 높게 나타나 그 이용성이 매우 다양할 것으로 생각된다. 또한 L. acidophilus SD 102는 ACE활성 억제효과가 18%로 시험균주 중에 가장 높았으며 L. acidophilus SD 103은 유단백질 유래 철분가용화 펩타이트 생성능이 높아 철분흡수율 증가와 연관된 probiotic 유산균으로서의 활용가능성이 있는 것으로 사료된다.

• 한국식품과학회지
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• 제38권5호
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• pp.691-698
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• 2006

본 연구는 혈액순환 개선 효능을 알아보기 위해 민간에서 빈번하게 사용하는 허브류인 마조람, 라벤더, 딜, 로즈마리, 히솝, 장미, 레몬밤, 파인애플 세이지 및 에키네시아 등 총 9종 을 실험 시료로 선정하였다. 이들을 부위별 및 용매별로 추출하여 angiotensin I converting enzyme(ACE) 저해 활성, hydroxy-methylglutalyl coenzyme A reductase 저해활성 및 혈전용해활성을 측정하였다. 추출 수율은 장미꽃이 가장 높았으며 열수 추출의 경우 43.3%였고 70% 에탄올 추출의 경우 45%였다. ACE 저해활성은 열수 추출의 경우 장미꽃이 133.8% 로 가장 높았고 70% 에탄올 추출의 경우에는 파인애플 세이지 잎이 91.2%로 가장 높았다. HMG-CoA reductase 저해활성의 경우에는 장미꽃이 열수 추출물 48.9%, 70% 에탄올 추출물 80.5%로 모두 높았다. 혈전용해활성도 장미꽃에서 열수 추출이나 에탄올 추출물 모두 가장 높은 활성을 보였다. 이상의 결과로 볼 때 장미꽃 추출물의 혈액순환 개선 효과가 뛰어난 것으로 나타나 기능성 식품으로의 개발 가능성이 기대된다.

• 한국식품과학회지
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• 제30권6호
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• pp.1476-1479
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• 1998

키토산 올리고당이 ACE 저해활성과 SHR의 혈압에 미치는 영향을 검토하였다. 키토산 올리고당은 모두 ACE 저해활성을 나타내었다. ACE 저해활성$(IC_{50})$은 3량체가 $0.9\;{\mu}mole$로 가장 우수하였고 2량체의 경우에는 $2.4\;{\mu}mole$, 그 외의 올리고당은 모두 $>100\;{\mu}mole$였다. 강력한 ACE 저해제인 Captopril(2-D-mercaptopropanoyl-L-proline)의 인체 투여량을 기준으로 3량체 키토산 올리고당 2.14 mg/kg을 SHR에 강제 경구투여한 바, 4시간 경에 8주령 및 21주령 SHR모두 최저혈압을 보였고 이 때의 혈압 강하는 8주령 SHR $27{\pm}4.8\;mmHg$, 21주령 SHR $36{\pm}4.3\;mmHg$로 나타났다. 따라서 3량체 키토산 올리고당은 2량체 키토산 올리고당과 함께 향후 고혈압 치료제로서 응용가능함이 시사되었다.

• 한국식품과학회지
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• 제29권6호
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• pp.1316-1318
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• 1997

산 casein으로부터 FPLC를 이용하여 gel permeation column으로 ${\kappa}-Casein$을 분획한 다음, 이를 chymosin, pepsin, trypsin 으로 각각 처리하여 3% TCA에서 soluble한 부분을 증류수로 투석(MW cut-off 1kDa)시킨 후 ACE저해 효과를 측정한 결과, trypsin으로 분해 시킨 경우 ACE 저해율이 94.7%로 가장 높게 나타났으며, chymosin 가수분해물은 가장 낮았다. GMP를 투석막의 종류에 따라 투석 시킨 후 $IC_{50}$을 측정한 결과, MW cut-off 의 크기가 증가할수록 ACE저해효과는 감소하는 것으로 나타났으며, MW cut-off 2 kDa의 경우가 가장 높은 저해율을 보였고 MW cut-off 5kDa에서는 저해율이 가장 낮았다.

• 동의생리병리학회지
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• 제23권1호
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• pp.93-96
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• 2009

This study was attempted to investigate the anti-hypertensive activity of Solanum tuberosum, cv. Gui valley. Ethanol extract of Gui vally (EGV) increased free radical scarvenge activity up to 91.6% of control at $200{\mu}g/m{\ell}$. It's anti-oxidant activity is similar to 10 uM of ascorbic acid, well known as antioxidant. EGV inhibited Angiotensin-I-converting enzyme (ACE) activity in vitro. ACE plays a important role in regulation of blood pressure and ACE inhibitors are important for the treatment of hypertension. Anti-hypertensive activity of EGV in vivo was assessed in lead acetate (LAT)-induced hypertensive rats for 8 weeks. Elevated blood pressure in control group was significantly decreased by EGV at 200 mg/kg. Also, ACE activity in blood was also suppressed by EGV treatment. Taken together, these results suggest that EGV has an anti-hypersive activity via inhibition of ACE and can be used for the treatment or prevention of hypertension.