• Food Science and Biotechnology
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• 제18권6호
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• pp.1476-1480
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• 2009

Antioxidant activities of mulberry leaf extracted with different concentrations of EtOH were investigated. Total phenolic content and electron donating abilities of extract from 70% EtOH were the highest. Extracts obtained from EtOH-water mixture were shown to be significantly higher superoxide dismutase (SOD)-like activities than other treatment (p<0.05). Angiotensin-converting enzyme (ACE) inhibition was the greatest at 50% EtOH concentration (p<0.05). The extracts from 30-70% EtOH exhibited higher ferric reducing ability of plasma (FRAP) value than rest of the concentration (p<0.05). In case of nitrite scavenging activity, much higher scavenging activities were observed when the extraction was performed with EtOH or EtOH-water mixture (p<0.05). The results indicate that concentration of EtOH as extraction solvents can affect the antioxidant activity of mulberry leaf, which may provide useful information on the optimal solvent conditions for the extraction.

• Applied Biological Chemistry
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• 제44권3호
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• pp.137-142
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• 2001

미생물로부터 고분자 물질을 생산하여 기능성 식품보조제로 사용하기 위하여 Bacillus coagulans CE-74에 의해 생산된 biopolymer를 이용해 glucose, bile acid 흡수 지연 효과 및 ACE와 tyrosinase 저해 효과를 조사 검토하였다. 분리된 biopolymer의 소화관내 생리적 기능성을 알아보기 위해 반투막을 이용한 in vitro 실험을 수행하였다. 투석막을 이용하여 biopolymer의 glucose흡수 지연 효과를 살펴본 결과 biopolymer를 2% 첨가한 경우 투석 30분이 경과되었을 때 히ucose retardation index가 43.5%였고 시간이 경과 할수록 그 효과는 감소 하였으며 투석 5시간 이후에는 그 효과가 거의 없는 것으로 나타났다. Bile acid흡수 지연 효과를 살펴본 결과 투석 30 분이 경과 되었을 때 bile acid retardation index는 biopolymer가 1%, 2% 첨가된 경우 각각 34%, 44.2%였으며 시간이 경과할수록 그 효과가 감소하였다. 분리된 biopolymer의 ACE저해효과는 $10\;{\mu}g/{\mu}l$의 농도에서는 97%의 저해를 보였으며, tyrosinase 저해효과를 관찰한 결과 $20\;{\mu}g/{\mu}l$농도에서 48.5%의 저해효과를 나타내었다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 추계 수산관련학회 공동학술대회발표요지집
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• pp.183-184
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• 2001

Angiotensin I converting enzyme (ACE) in renin-angiotensin system is a cause of essential hypertension, which covers most hypertension, one of the major adult diseases. Thus, the inhibition of ACE would be indispensable for the prevention and cure of hypertension. Therefore, a lot of studies on the ACE inhibitor have been conducted. Peptides from the protein hydrolysate have been reported as an remarkable inhibitor. Especially, various ACE inhibitory peptides were isolated and identified from marine products for their utilization as value added products. (omitted)

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1315-1320
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• 2015

To enhance the γ-aminobutyric acid (GABA) content, the optimized fermentation of soybean with added sea tangle extract was evaluated at 30℃ and pH 5.0. The medium was first inoculated with Aspergillus oryzae strain FMB S46471 and fermented for 3 days, followed by the subsequent inoculation with Lactobacillus brevis GABA 100. After fermentation for 7 days, the fermented soybean showed approximately 1.9 g/kg GABA and exhibited higher ACE inhibitory activity than the traditional soybean product. Furthermore, several peptides in the fraction containing the highest ACE inhibitory activity were identified. The novel fermented soybean enriched with GABA and ACE inhibitory components has great pharmaceutical and functional food values.

• Journal of Applied Biological Chemistry
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• 제49권3호
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• pp.82-85
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• 2006

Eucommia ulmoides bark extracts by cold water, boiling water, 100% EtOH, 70% EtOH, 100% MeOH, 70% MeOH and $CHCl_3$ were assayed for their medicinal effects. The antioxidant activity of the extracts ranged from $IC_{50}$ 125.2 to $IC_{50}\;872.7{\mu}g/ml$ in the 1,1-diphenyl-2-picrylhydrazyl (DDPH) free radical-scavenging assay, and cold water extracts had the highest antioxidant activity. $CHCl_3$ extracts had the highest inhibitory effect on angiotensin I-converting enzyme (ACE) giving inhibition of up to 56.4% at a concentration of 1 mg/ml. Extracts in 100% EtOH had the greatest inhibitory effect on $\acute{a}-amylase$ activity ($IC_{50}=174.6{\mu}g/ml$), and 70% MeOH extracts had the greatest inhibitory effect on ${\alpha}-glucosidase$ activity ($IC_{50}=14.0{\mu}g/ml$). Taken together, these results provided the in vitro evidence on the ACE, amylase and glucosidase inhibitory actions of E. ulmoides bark that form the pharmacological basis for its antihypertensive and antidiabetic action.

• 한국축산식품학회지
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• 제26권4호
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• pp.511-516
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• 2006

발효 소시지의 제조에 starter로 사용한 균주를 선발하기 위해 각 유산균 균주들의 ACE 저해 활성, 콜레스테롤 흡착능력, 발효 육제품 저장 중 S. aureus에 대한 저해 황성을 평가하였다. ACE 저해 활성은 L. plantarum L167이 가장 우수하였고(58.75%), 콜레스테롤 흡착 능력이 우수한 균주로는 L. plantarum L155를 선발하였다. 박테리오신을 생산하는 P. damnosus를 starter로 이용하여 제조한 발효 소시지에 S. aureus를 접종하여 상온 저장 중 생균수를 측정한 결과 저장 35일째에 대조구에 비하여 1 log의 균수 감소 효과가 나타났다.

• 한국식품조리과학회지
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• 제24권6호
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• pp.849-855
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• 2008

DPPH radical scavenging activities were 66.80% by hot water extract and 41.86% by 80% methanol extract. Inhibitions of angiotensin I-converting enzyme (ACE) were 91.09% by hot water extract and 75.20% by 80% methanol extract. In this study, Sulgidduk samples were prepared with 1, 3, 5, and 7% concentrated sweet pumpkin powder (CSPP), and a control were examined for quality characteristics such as moisture content, water activity, color, textural characteristics, and sensory qualities, in order to determine the optimal ratio of CSPP in the formulation. The samples containing CSPP exhibited significantly higher moisture content than the control group. Water activity was not significantly different among the Sulgidduk samples. For the color, the control group presented significantly higher lightness as compared to the samples containing CSPP. The samples containing 1 and 3% CSPP had significantly higher greenness than the samples containing 5 and 7% CSPP. Yellowness increased as the level of CSPP content increased. In terms of textural characteristics, hardness, gumminess, and chewiness were highest at the 1% substitution level, while lowest at the 3% level. The CSPP samples presented significantly higher adhesiveness, springiness, and cohesiveness than the control group. In the consumer acceptance and characteristic intensity rating test, the control group showed significantly higher color and flavor as compared to the CSPP samples. Pumpkin flavor, delicious taste, and off-flavor increased with increasing amounts of CSPP. Softness, overall acceptability, and gumminess were not significantly different among the various samples tested. Sweetness was highest at the 5% substitution level, while lowest at the 1% level. In conclusion, the results indicate that substituting $3{\sim}5%$ CSPP to Sulgidduk is optimal, providing good DPPH radical scavenging activity and inhibition of ACE as well as reasonably high overall acceptability.

• Applied Biological Chemistry
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• 제49권2호
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• pp.114-124
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• 2006

생리활성을 갖는 새로운 기능성 물질을 개발할 목적으로 108종의 뽕잎을 이용하여 물과 80% 에탄올 추출물을 제조하고 이들의 Angiotensin converting enzyme(ACE) 활성억제효과 및 XOase(xanthine oxidase) 활성억제효과를 탐색하여 본 결과, 페놀함량이 물 추출물에서는 각자용산, 대정선 등 10종이, 80% 에탄올 추출물에서는 와룡, 하수상 등 23종이 15 mg/g 이상의 총 페놀함량을 나타내었으며, Angiotensin converting enzyme (ACE) 활성억제효과는 야마나까다까스께, 미지로 등 10종이 물 추출물 및 80% 에탄올 추출물에서 동시에 100%의 활성억제효과를 나타내었으며, 그 외 청일뽕 등 34종은 물 추출물에서, 원주고조 등 34종은 80% 에탄올 추출물에서 90% 이상의 활성억제효과를 나타내었다. 또한 XOase(xanthine oxidase) 활성억제효과는 물 추출물에서 청십조생, 수원상3호 등 5종이, 80% 에탄올 추출물에서 적목이 50% 이상의 활성억제효과를 나타내었으며, 그 외 수원뽕 등 27종은 물 추출물에서, 홍올뽕 등 21종은 80% 에탄올 추출물에서 약간씩의 효과를 나타내었다. 이상의 결과, g당 페놀함량이 적은 뽕잎이지만 높은 생리활성기능을 나타내는 여러 종의 뽕잎 물과 80% 에탄올 추출물들은 항고혈압 및 항관절염의 효과가 있는 기능성 식품소재로 활용할 수 있을 것으로 판단되었다.

• 생명과학회지
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• 제28권11호
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• pp.1290-1300
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• 2018

돌산갓김치의 조추출물을 이용하여 glucosinolates함량, 항산화활성, 항고혈압 및 항당뇨활성을 측정하였다. Glucosinolates 함량은 DLMK의 잎과 줄기에서 6.41, 7.92 mg/g으로 발효기간 중 낮은 함량을 나타냈다. Total polyphenol과 total flavonoid함량은 줄기보다 잎에서 발효기간 동안 2배 이상의 함량을 나타냈다. ABTS라디컬 소거활성과 EDA은 발효 14일째 대조군과 유사한 활성을 나타냈고, FRAP는 발효 14일째 잎에서 대조군보다 높은 함량을 나타냈다. 그리고 항고혈압 활성(ACE 저해활성)은 잎에서 대조군인 0.01% captopril과 비슷하거나 높은 저해활성을 나타냈으며, 항당뇨활성(${\alpha}$-glucosidase 저해활성)은 대조군인 0.05% acarbose보다 잎, 줄기에서 높은 저해활성을 나타냈다. 또한 glucosinolates 함량과 ABTS 및 EDA, ACE 저해활성 및 ${\alpha}$-glucosidase 저해활성의 상관관계는 줄기 추출물 보다 잎 추출물이 높은 양의 상관관계를 나타냈다. 발효기간 중 glucosinolates는 sinigrin, glucobrassicin, glucotropaeolin, progoitrin 검출되었다. 이 결과를 통해 돌산 갓김치 추출물은 항산화, 항고혈압 및 항당뇨활성에 효과가 높은 것으로 나타냈기에 기능성 식품으로서 가치가 높을 것으로 기대된다.

• 한국작물학회지
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• 제55권2호
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• pp.159-164
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• 2010

Specialty barley extracts were prepared and investigated for its antioxidant activity and biological activity. Hunter $L^*$ values of the Iksan 86 extracts had higher than that of the Iksan 87 and Zasoojeongchal extracts. The extraction yields of Iksan 86, Iksan 87, and Zasoojeongchal was 8.08, 6.62, and 7.30%, respectively. The contents of total phenolic compounds of the Iksan 86, Iksan 87, and Zasoojeongchal extracts were 16.24, 15.51, and 13.95 GAE mg/g of sample, respectively. The DPPH radical scavenging activity of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 50.00, 33.27, and 7.56% at a 500 ppm, respectively. The samples showed an inhibition of xanthin oxidase. ACE inhibition effect of specialty barley extracts, Iksan 86, Iksan 87, and Zasoojeongchal, was 39.81, 41.06, and 27.78%, respectively. Tyrosinase inhibition rates (%) of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 26.21, 24.57, and 20.00%, respectively. Results indicated that specialty barley extracts possesses various biological activities including antioxidative capacity, xanthin oxidase inhibition activity, angiotensin converting enzyme inhibition activity, and tyrosinase inhibition activity.