• Proceedings of the Botanical Society of Korea Conference
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• 1999.07a
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• pp.11-15
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• 1999

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1,700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.356-361
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• 2005

In this paper, a new scheme of current programmed control for three phase PWM AC-AC converter is presented. Compared to duty-ratio voltage control, current programmed control has several advantages such as reduction of system order and inherent current protection. By considering only the magnitude components, the same scheme in the DC-DC converter can be extended to the three phase PWM AC-AC converter. Sensing the output voltage and the inductor current, an error signal will be generated by comparing the output voltage magnitude with a reference value. Then the error signal will be processed by a PI compensator to generate the current command. The converter switches will the change the state by a periodic clock pulse or at the instants when the inductor current magnitude reaches the current command. As an example case, the buck converter is treated. The converter analysis is carried out by applying the complex DQ circuit transformation to obtain basic physical insight into the converter operation and to establish some important characteristic equations for control purpose. The simulation with Simplorer shows the validity of the proposed scheme and the experimental results support the verification of the design.

• Journal of Power Electronics
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• v.11 no.6
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• pp.897-903
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• 2011

Many voltage sag compensators have been introduced, including the traditional dynamic voltage restorer (DVR), which requires an energy storage device but is inadequate for compensating deep and long-duration voltage sags. The AC-AC sag compensators introduced next do not require a storage device and they are capable of compensating voltage sags. This type of compensator needs an AC-AC converter to regulate the output voltage. Presented in this paper is a three-phase PWM-switched autotransformer voltage sag compensator based on an AC-AC converter that uses a proposed detection technique and PWM voltage control as a controller. Its effectiveness and capability in instantly detecting and compensating voltage sags were verified via MATLAB/Simulink simulations and further investigated through a laboratory prototype developed with a TMS320F2812 DSP as the main controller.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.7
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• pp.1349-1355
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• 2009

This paper is studied on a novel power factor correction (PFC) AC-DC converter of high efficiency by soft switching technique. The input current waveform in the proposed converter is got to be a sinusoidal form composed of many a discontinuous pulse in proportion to the magnitude of a ac input voltage under the constant switching frequency. Therefore, the input power factor is nearly unity and the control method is simple. The proposed converter adding an electric isolation operates with a discontinuous current mode (DCM) of the reactor in order to obtain some merits of simpler control, such as fixed switching frequency, without synchronization control circuit used in continuous current mode (CCM). To achieve the soft switching (ZCS or ZVS) of control devices, the converter is constructed with a new loss-less snubber for a partial resonant circuit. It is that the switching losses are very low and the efficiency of the converter is high, Particularly, the stored energy in a loss-less snubber capacitor recovers into input side and increases input current from a resonant operation. The result is that the input power factor of the proposed converter is higher than that of a conventional PFC converter. This paper deals mainly with the circuit operations, theoretical, simulated and experimental results of the proposed PFC AC-DC converter in comparison with a conventional PFC AC-DC converter.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.8
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• pp.1685-1691
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• 2004

The objective of this study is aimed at reducing the contour error of AC Servo derives by improving the interpolation error of each axis through variable structure control system. The errors in machining process by AC Servo motor are due to many elements, such as the delay of the servo drivers, friction and the gain mismatch between x axis and y axis motors and so on. Sliding mode control system is applied to a AC servo drive as a numerical example in this paper. The experiment results which are compared with those of typical PI scheme show the validity of improvement in circular interpolation error of the system.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.497-502
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• 2013

Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cystforming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess a type of Atg8. In this study, an isoform of Atg8 was identified and characterized in Acanthamoeba castellanii (AcAtg8b). AcAtg8b protein was found to encode 132 amino acids and to be longer than AcAtg8 protein, which encoded 117 amino acids. Real-time PCR analysis showed high expression levels of AcAtg8b and AcAtg8 during encystation. Fluorescence microscopy demonstrated that AcAtg8b is involved in the formation of the autophagosomal membrane. Chemically synthesized siRNA against AcAtg8b reduced the encystation efficiency of Acanthamoeba, confirming that AcAtg8b, like AcAtg8, is an essential component of cyst formation in Acanthamoeba. Our findings suggest that Acanthamoeba has doubled the number of Atg8 gene copies to ensure the successful encystation for survival when 1 copy is lost. These 2 types of Atg8 identified in Acanthamoeba provide important information regarding autophagy formation, encystation mechanism, and survival of primitive, cyst-forming protozoan parasites.

• Herbal Formula Science
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• v.19 no.2
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• pp.23-38
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• 2011

Objectives : Atractylodes chinensis(AC) and Phellodendron amurense Ruprecht(PAR) have been used as anti-inflammatory medicines. However, the effects of AC, PAR and Yimyosan on AP were not examined. To prove this, We decocted the dried prescription of AC and PAR with boiling distilled water and freeze-dried to be powdered. AC, PAR and Yimyosan was administrated intraperitoneally. Methods : 1h after administration, cerulein was injected hourly six times. 6hrs after last cerulein injection, mice were sacrificed, then the pancreas and blood were harvested. Serum amylase and lipase, neutrophil infiltration, pancreatic cytokines were used as the parameter of severity of AP. Results : As a result of assessment the parameters of AP, AC alone treatment did not inhibit the severity of AP, however PAR treatment inhibited the severity of AP significantly. Yimyosan also showed the protective effects against AP at lower doses, however AC alone plus PAR alone extract did not show the protective effects significantly. Conclusions : In conclusion, PAR extract has a protective effects on AP, and the effects could be increased by co-treatment with AC.

• The Journal of Information Technology
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• v.3 no.3
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• pp.57-65
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• 2000

We have proposed BIST method and circuit for embedded 16M SDRAM with logic. It can test the AC parameter of embedded 16M SDRAM using the BIST circuit capable of detecting the address of a fail cell of a 16M SDRAM installed in an Merged Memory with Logic(MML) generating the information of repair for redundancy circuit. The function and AC parameter of the embedded memory can also be tested using the proposed BIST method. The total gate of the BIST circuit is approximately 4,500 in the case of synthesizing by $0.25\mu\textrm{m}$ cell library. and verify the result of Verilog simulation. The test time of each one AC parameter is about 200ms using 2Y-March 14N algorithm.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.11a
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• pp.242-242
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• 2009

Fabrication of Anodic aluminum oxide under DC vias condition has been studied. When bias and time of anodic aluminum oxide process change, the hole distance and diameter size change. Comparison of fabricated AAO between AC vias and DC vias condition has been studied in this experiment. The first and second anodization of one aluminum is done by using DC and AC power supplier. And first and second anodization of another aluminum is done by DC power supplier. The size of the aluminum is $1cm{\times}3cm$, and second anodic aluminum oxide process takes about 45min. It is found that the hexagonal shape appears on the surface of the AAO. AC power source can fabricate aao which have a nano hole array. We can see that the hole on the surface of the AC vias has a better rounded hole than DC vias AAO. we need more data so we can get characteristic about AC power generated AAO.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.555-558
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• 2017

In this paper, we designed a power supply AC LED driving device which can display uniform power consumption and light efficiency even when AC power of different size is applied. By setting input voltages and deviations of different sizes, it is confirmed that the electric light characteristics are the same at two input voltages. In addition, according to peak voltage of AC power, improper lighting caused in specific LED section is improved, and LED lighting of all sections is implemented in all commercial AC input voltage.