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Physiological responses involved in reactive oxygen species (ROS) of rice plant under alone or multi artificial stress conditions

  • Kim, Yoonha;Waqas, Muhammad;Khan, Abdul Latif;Mun, Bong-Gyu;Yun, Byung-Wook;Lee, In-Jung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.203-203
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    • 2017
  • The Earth's climate is rapidly changing because of increasing carbon dioxide content in atmosphere so, climate prediction models anticipate that earth surface temperature will rise by 3 to $5^{\circ}C$ in next 50 to 100 years. Therefore, frequency of un-expected weather events such as drought, salinity, low or high temperature and flooding etc. will be increasing worldwide. Furthermore, increased atmosphere temperature can influence pests and pathogens spread as well. Therefore, to protect enormous grain loss from unexpected weather conditions, studies related with combine stress conditions like abiotic plus biotic stress condition are really required. Thus, our research focused on physiological responses under combined abiotic and biotic stress condition in rice plant. To induce uniform stress condition, we used NaCl (100 mM) and salicylic acid (0.5 and 1.0 mM SA) as each stress a stimulator. Each artificial abiotic and biotic stress inducer was applied to hydroponically grown rice seedlings alone or together for four day. The data were collected in a time-dependent manner [1, 2, 3 and 4 day(s) after treatment (DAT)] and were matched with our anticipation that shoot length and shoot fresh weight was decreased in solo and combined abiotic and biotic stress condition. The lipid peroxidation content was significantly increased ($1.5{\pm}0.2$ to $2.7{\pm}0.1mg$ mg of $MDA\;g^{-1}FW$) in the first two days in both stress exposed plants, and showed the opposite trend ($0.5{\pm}0.01$ to $0.1{\pm}0.001mg$ of $MDA\;g^{-1}FW$) in last two days under multi stress condition. Superoxide dismutase (SOD) activity did not showed difference in only biotic stress condition (alone 0.5 and 1.0 mM SA) as compared to control however, it was significantly increased in multi stress condition or solo abiotic stress condition whereas, catalase (CAT), and ascorbate peroxidase (APX) activities were significantly decreased in solo biotic and combined abiotic and biotic condition. In particular, both enzymes activities were more decreased in multi stress condition as compared to solo biotic stress condition. The results for relative mRNA expression level of CAT and APX enzymes were in agreement with results of spectrophotometric values. Correlation value between each stress condition and phenotypic data showed that biotic stress condition showed high correlation with activity of CAT and APX whilst, abiotic stress condition revealed significant correlation with SOD activity.

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Quality Characteristics and Antioxidant Activities of Commercial Jeupjang (일부 시판 즙장의 품질 특성 및 항산화 활성)

  • Kim, Ha-Yun;Hwang, In-Guk;Yoo, Seon-Mi;Hwang, Young;Cha, Sung-Mi;Kim, Haeng-Ran
    • The Korean Journal of Community Living Science
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    • v.21 no.4
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    • pp.571-579
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    • 2010
  • Jeupjang has long been considered only a local delicacy. So, it has gradually been forgotten because its name and definition have not been established. This study was performed in order to provide some basic data by comparing the general components, color, free amino acids, free sugar, organic acids and antioxidant activities between commercial Jeupjang(GG, GD, JM, NJ, NS). Moisture contents, crude protein, crude fat and crude ash contents of Jeupjang were 48.07~61.77%, 4.08~10.53%, 3.92~4.64%, and 3.63~9.95%. pH, titrable acidity and salt in Jeupjang were 4.32~5.50, 9.37~14.82 mL(1N-NaOHmL), and 3.27~8.90%. Amino-type nitrogen content is 42.05~ 138.83mg%. Lightness(L), redness(a) and yellowness(b) values in color of Jeupjang were10.04~21.89, 18.92~28.80, and 17.16~37.56. The total content of free amino acids in commercial Jeupjang ranged from 35.22mg% to 306.02mg% in which it showed wide variation among samples. The major free amino acids were glutamic acid, aspartic acid, alanine and proline. The major free sugars were fructose(0.31~1.60%), glucose(6.20~ 13.61%) and maltose(7.34~10.01%). The major organic acids were succinic acid(29.08~ 103.62 mg%) and citric acid(3.08~108.69 mg%). Total polyphenol contents were from 1.16 mg/g to 1.78 mg/g, DPPH radical scavenging activity is from 0.57 AEAC to 1.53 AEAC and ABTS radical scavenging activity is from 1.58 AEAC to 2.85 AEAC. This result might cause the differences of soybean composition, processing methods and brewing periods. For the succession of traditional fermented food such as Jeupjang, quality standards are required. To this end, the standardization of manufacturing processes must be followed.

A Survey on Clients' Home Care Satisfaction in Taejon City in Korea (일개 시지역의 가정간호 수혜자들의 만족도 조사)

  • Kim, Sun-Sook;So, Hee-Young;Lee, Tae-Yong
    • Journal of Korean Academic Society of Home Health Care Nursing
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    • v.5
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    • pp.73-83
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    • 1998
  • This study enrolled one thousand five hundred twenty one clients that were receiving home care services at five health centers and a home care services center in the city of Taejon from November 17 through December 7, 1997. The purpose of this study was to analyze satisfaction levels, general characteristics and efficacies, of the client served home care. The main results were as follows; The age group of most of the subjects was 70-79 years: 76.9% of them were female and 87.3% were over 65 years. In education variables, over 90% of the clients were below primary school. In marital status variables, 63.4% of them had no spouse, 57.5% of them were widows and widowers. In living arrangement variables, alone(35.8%) was the most. In insurance status variables, medical aid(69.9%) was the most. In household income variables, below 000 won(72.6%) was the most. In hospitalization variables, 53% of the clients had no hospitalization. The satisfaction level of home care service of male and female was similar. The older the age, the higher the satisfaction level. The satisfaction level of the group having religion was higher than the group having no religion and the Christianity group had the highest satisfaction level(p<0.001). In education variables, the over high school group was the highest satisfaction level(p<0.01). In living arrangement variables, the other (sisters or neighbors etc.) group indicated the highest satisfaction level(p<0.001). In insurance status variables, the other group(except for medical aid) was the highest satisfaction level(p<0.001). In household income variables, below 390,000 won was the highest satisfaction level(p<0.001). In hospitalization variables, 'over 4 times' group indicated the highest satisfaction level (p<0.01). Home care took place more in health centers than in home care services center. In frequency of home care per month three times was the most. In opinion of home care frequency per month 82.8% of the people answered 'proper'. A lot of present illness was neuralgia, arthritis, digestive disease, hypertension & CVA. In contents of home care services variables, education & counselling was the most and medication was second. In duration of illness variables, over 10 years was the most. In place of treatment before home care service variables, hospital(57%) was the most. In illness condition after home care variables, 'moderate' was the most and 'much better'(85.5%) was second. In help of home care variables, 'much help'(71.5%) was the most and 'moderate'(28.1%) was second. In contents of counselling variables, treatment method of illness was the most. Home care services center indicated higher level of satisfaction than health center(p<0.001). In opinion of home care frequency per month variables, 'less' was the highest satisfaction level (p<0.001). In duration of illness variables, below one year was the highest and over 10 years was second(p<0.001). In place of treatment before home care service variables, health center was the highest (p<0.001). In illness condition after home care variables 'much better' was the highest and 'worse' was second (p<0.001). I n help of home care variables, 'much help' was the highest (p<0.001). In contents of counselling variables, cause of illness was the highest(p<0.001). According to the 14 items which consisted of 3 point scales the total level of satisfaction of home care service was very high, with total mean score 36. According to the above results, most clients taking home care services are satisfied. However, organization and a fund are required to support high quality home care services to those who need them. Furthermore, a follow-up survey should be accomplished to evaluate the status of clients.

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Effect of Pressure Rise Time on Tidal Volume and Gas Exchange During Pressure Control Ventilation (압력조절환기법에서 압력상승시간(Pressure Rise Time)이 흡기 일환기량 및 가스교환에 미치는 영향)

  • Jeoung, Byung-O;Koh, Youn-Suck;Shim, Tae-Sun;Lee, Sang-Do;Kim, Woo-Sung;Kim, Dong-Soon;Kim, Won-Dong;Lim, Chae-Man
    • Tuberculosis and Respiratory Diseases
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    • v.48 no.5
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    • pp.766-772
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    • 2000
  • Background : Pressure rise time (PRT) is the time in which the ventilator aclieves the set airway pressure in pressure-targeted modes, such as pressure control ventilation (PCV). With varying PRT, in principle, the peak inspiratory flow rate of the ventilator also varies. And if PRT is set to a shorter duration, the effective duration of target pressure level would be prolonged, which in turn would increase inspiratory tidal volume(Vti) and mean airway pressure (Pmean). We also postulated that the increase in Vti with shortening of PRT may relate inversely to the patients' basal airway resistance. Methods : In 13 paralyzed patients on PCV (pressure control 18$\pm$9.5 cm $H_2O$ $FIO_2\;0.6\pm0.3$, PEEP 5$\pm$3 cm $H_2O$, f 20/min, I : E1 : 2) with Servo 300 (Siemens-Elema, Solna, Sweden) from various causes of respiratory failure, PRT of 10 %, 5 % and 0 % were randomly applied. At 30 min of each PRT trial, peak inspiratory flow (PIF, L/sec), Vti (ml), Pmean (cm $H_2O$) and ABGA were determined. Results : At PRT 10%, 5%, and 0%, PIF were 0.69$\pm$0.13, 0.77$\pm$0.19, 0.83$\pm$0.22, respectively (p<0.001). Vti were 425$\pm$94, 439$\pm$101, 456$\pm$106, respectively (p<0.001), and Pmean were 11.2$\pm$3.7, 12.0$\pm$3.7, 12.5$\pm$3.8, respectively (p<0.001). pH were 7.40$\pm$0.08, 7.40$\pm$0.92, 7.41$\pm$0.96, respectively (p=0.00) ; $PaCO_2$ (mm Hg) were 47.4$\pm$15.8, 47.2 $\pm$15.7, 44.6$\pm$16.2, respectively (p=0.004) ; $PAO_2-PaO_2$ (mm Hg) were 220$\pm$98, 224$\pm$95, 227$\pm$94, respectively (p=0.004) ; and $V_n/V_T$ as determined by ($PaCO_2-P_E-CO_2$)/$PaCO_2$ were 0.67$\pm$0.07, 0.67$\pm$0.08, 0.66$\pm$0.08, respectively (p=0.007). The correlation between airway resistance and change of Vti from PRT 10% to 0% were r= -0.243 (p=0.498). Conclusion : Shortening of pressure rise timee during PCV was associated with increased tidal volume, increased mean airway pressure and lower $PaCO_2$.

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Antimicrobial and Antioxidant Activity of the Discorea alata L. (Discorea alata L.의 항균 및 항산화 활성)

  • Kwon, Jeong-Eun;Kwon, Jung-Bae;Kwun, In-Sook;Sohn, Ho-Yong
    • Microbiology and Biotechnology Letters
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    • v.38 no.3
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    • pp.283-288
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    • 2010
  • Yam (Dioscorea spp.) has been used as important edible and medicinal natural resource in worldwide and D. alata L. is most popular nourishment among the yam. In this study the composition, color, antioxidation and antimicrobial activity of D. alata Gyeongbuk No. 6 (GB-6), which was established in Gyeongbuk Agricultural Research & Extension Services, Andong, Korea, was compared to those of D. batatas Gyeongbuk No. 1 (GB-1), a major domestic cultivation species. Water content of GB-6 was $78.02{\pm}0.16%$, which is slightly lower than that of GB-1 ($82.6{\pm}0.07%$). The contents of crude protein, crude fat, crude fiber and ash of GB-6 were 0.95, 0.26, 0.85 and 0.70%, whereas those of GB-1 were 1.58, 0.15, 1.39 and 0.88%, respectively. Analysis of color using colormeter showed that the GB-6 is slight dark-yellow than GB-1, and total polyphenol content of GB-6 was 2-fold higher compared than that of GB-1. Sequential organic solvent fractions from methanol extract of GB-6 showed that the ethylacetate fraction has highest total polyphenol ($144.1{\pm}3.20\;mg/g$). Determination of antioxidation activity showed that the ethylacete fraction and water fraction has strong DPPH radical scavenging activity ($IC_{50}=78.32\;{\mu}g/mL$) and reducing power, respectively. In antimicrobial activity assay, the n-hexane and ethylacetate fraction showed antibacterial activity against B. subtilis, L.monocytogenes, S. epidermidis, S. aureus, P. vulgaris, and S. typhimurium. These results provide the possibility of domestic cultivated D. alata GB-6 as a healthy food.

Changes in quality characteristics of makjang depending on fermentation location and complex starters (발효 장소와 복합 종균에 따른 막장의 품질 특성 변화)

  • Jieon Park;Myeong-Hui Han;Woosoo Jeong;Soo-Hwan Yeo;So-Young Kim
    • Food Science and Preservation
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    • v.30 no.6
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    • pp.1056-1071
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    • 2023
  • This study aimed to investigate the quality and microbial population changes for 90 days under two fermentation conditions, outdoors and indoors (35℃), with starters (single or mixed) in soybean paste. Bacillus velezensis NY12-2 (S1), Debaryomyces hansenii D5-P5 (S2), Enterococcus faecium N78-11 (S3), and their mixtures (M) were used for the makjang fermentation. The content of amino-type nitrogen among the makjang samples was highly shown in the indoors, followed by M, S3, and S2. The glutamic and aspartic acid contents in the M sample fermented in the indoors showed the highest values of 867.42±77.27 and 243.20±15.79 mg/g, respectively. By the electronic tongue analysis, the M sample fermented in the indoors exhibited lower saltiness and higher umami than the others. Consequently, we expect that using mixed strains, such as Bacillus, Debaryomyces, and Enterococcus, under constant conditions showed potential to the quality improvement of soy products.

Effects of Dietary Barley on the Growth Performance and Carcass Characteristics in Hanwoo Steers (보리의 사료가치평가와 한우 거세우 급여에 의한 발육 및 육질개선효과 구명)

  • Chang, S.S.;Oh, Y.K.;Kim, K.H.;Hong, S.K.;Kwon, E.G.;Cho, Y.M.;Cho, W.M.;Eun, J.S.;Lee, S.C.;Choi, S.H.;Song, M.K.
    • Journal of Animal Science and Technology
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    • v.49 no.6
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    • pp.801-818
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    • 2007
  • This study was carried out to determine the effects of processing methods of barley as a proper source of grain in diets of Hanwoo on fermentation pattern in the rumen fluid and digestibility of the diets, the growth performance and carcass characteristics of Hanwoo. The degree of ruminal pH change in the cows fed diets of the Corn(corn basis) and GDRB(ground and dry-rolled barley) tended to be greater than those in the cows fed other two diets (ground barley, GB and dry-rolled barley, DRB). The diet of GDRB showed the lowest ruminal pH(5.5), indicating the rapid degradation of the diet in the rumen. Although ammonia concentration was not affected by diet, the GR and DBR diets maintained the low concentrations of ruminal ammonia compared with other two treatments. And the effects of feeding processed barley grain on body weight gain and meat quality of Hanwoo steers were as follows. Steers fed DRB diet had the highest body weight, 683.0kg at 28 months old, while those fed the GDRB showed the lowest body weight, 653.3kg. The average daily gain(ADG) was similar between the steers fed Corn and GR throughout the whole period, but the GDRB showed the lowest ADG. The steers fed the DRB showed the significant increase in ADG(0.89kg/d from 19 to 23 months old and 0.43kg/d from 24 to 28 months old) compared with those fed other diets. Feeding diets containing corn and/or barley did not influence live body weight, cold carcass weight, carcass yield, back-fat thickness and carcass grade of Hanwoo steers.

Effect of Red Pepper Canopy Coverages on Soil Loss and Runoff from Sloped Land with Different Transplanting Dates (경사지에서 고추 정식시기에 따른 토양유실과 유출수에 대한 식생피복 효과)

  • Cho, H.R.;Ha, S.K.;Hyun, S.H.;Hur, S.O.;Han, K.H.;Hong, S.Y.;Jeon, S.H.;Kim, E.J.;Lee, D.S.
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.3
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    • pp.260-267
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    • 2010
  • As sloped farmland is subject to runoff and soil erosion and consequently require appropriate vegetative coverage to conserve soil and water, a field study was carried out to evaluate the impact of crop canopy coverage on soil loss and runoff from the experimental plot with three different textural types (clay loam, loam, and sandy loam). The runoff and soil loss were examined at lysimeters with 15% slope, 5 m in length, and 2 m in width for five months from May to September 2009 in Suwon ($37^{\circ}$ 16' 42.67" N, $126^{\circ}$ 59' 0.11" E). Red pepper (Capsicum annum L. cv. Daechon) seedlings were transplanted on three different dates, May 4 (RP1), 15 (RP2), and 25 (RP3) to check vegetation coverage. During the experimental period, the vegetation coverage and plant height were measured at 7 day-intervals and then the 'canopy cover subfactor' (an inverse of vegetation cover) was subsequently calculated. After each rainfall ceased, the amounts of soil loss and runoff were measured from each plot. Under rainfall events >100 mm, both soil loss and runoff ratio increased with increasing canopy cover subfactor ($R^2$=0.35, p<0.01, $R^2$=0.09, p<0.1), indicating that as vegetation cover increases, the amount of soil loss and runoff reduces. However, the soil loss and runoff were depending on the soil texture and rainfall intensity (i. e., $EI_{30}$). The red pepper canopy cover subfactor was more highly correlated with soil loss in clay loam ($R^2$=0.83, p<0.001) than in sandy loam ($R^2$=0.48, p<0.05) and loam ($R^2$=0.43, p<0.1) plots. However, the runoff ratio was effectively mitigated by the canopy coverage under the rainfall only with $EI_{30}$<1000 MJ mm $ha^{-1}hr^{-1}$ ($R^2$=0.34, p<0.05). Therefore, this result suggested that soil loss from the red pepper field could be reduced by adjusting seedling transplanting dates, but it was also affected by the various soil textures and $EI_{30}$.

EFFECTS OF INTERCROPPING, SEEDLING RATE AND FERTILIZER ON FODDER PRODUCTION IN THE LOW LYING AREA OF BANGLADESH

  • Sarker, N.R.;Giasuddin, M.;Islam, M.M.;Rahman, M.M.;Yasmin, L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.1
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    • pp.57-61
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    • 1994
  • The study was conducted at low lying areas in Pabna Sirajgong districts of Bangladesh. To observe the potentiality of bio-mass production two trials were conducted. In first trial maize intercropped with Khesari taking 15 experimental plots of each size $5m{\times}5m$ were arranged in 5 blocks having homogenous soil characteristics. The study showed that the bio-mass yield of sole maize and sole Khesari were 35.25 t/ha. and 56.80 t/ha. respectively and there was a significant difference (p < 0.01) among them. The results also showed that bio-mass yield of maize and Kherasi was higher ($70.04{\pm}6.25t/ha$, $98.88{\pm}10.77t/ha$ and $80.56{\pm}9.5t/ha$) compared to sole maize and sole Khesari and land equivalent ratio was also lower. For second trial, one hectare of land was divided into 16 experimental plots with 4 replications in each plot. Four levels of urea (0 kg/ha, 30 kg/ha, 45 kg/ha, and 60 kg/ha.) were applied to experimental plot. The seed rates were 98.8 kg/ha (farmer's practice), 86.45 kg/ha, 74.1 kg/ha and 61.75 kg/ha. average bio-mass yield of matikalai at different seed rates along with urea fertilizer ranged from 38.49 t/ha, to 65.35 t/ha. the highest seed rate along with highest fertilizer also correspond to the peak production (65.35 t/ha) and the lowest seed rate (61.75 kg/ha) along with the lowest fertilizer rate (30 kg/ha.) showed lowest production (38.49 t/ha.). Here, it was found that the bio-mass yield of matikalai increased with the incremental amount of seed, indicating significant effect (p < 0.05) of seed rates on the bio-mass yield of matikalai. On the other hand, fertilizer doses in different treatment combinations had significant effect (p < 0.05) on bio-mass yield. Two levels of seed rates at zero level of fertilizer were recommended : 86.45 kg/ha for the resource rich farmers and 61.75 kg/ha for the resource poor farmers.

Effect of Intra-Uterine Infusion of Lipopolysaccharides on Reproductive Performance in Dairy Cows with Retained Placenta (후산정체 젖소에 있어서 Lipopolysaccharide의 처리가 번식 성적에 미치는 영향)

  • Baek, Kwang-Soo;Park, Seong-Jai;Park, Soo-Bong;Lee, Wang-Sik;Kim, Hyeon-Shup;Jeong, Gyeong-Yong;Ki, Kwang-Seok;Jeon, Byeong-Soon;Ah, Byeong-Seog;Suh, Guk-Hyun;Kim, Tae-Il
    • Reproductive and Developmental Biology
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    • v.30 no.3
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    • pp.195-199
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    • 2006
  • This study was carried out to investigate the effect intra-uterine infusion of lipopolysaccharides(LPS) on the reproductive performance of dairy cows with retained placenta, The LPS isolated from Bacteroids helcogenes and Fusobacterium varium was injected at the rate of 100 ${\mu}g$ with 30 ml of phospahte buffer saline in each cow(n=33) at day 20 postpartum. Twelve cows with retained placenta were not heated with LPS and served as a control. Conception rate after first service was 0% and 33,3% for control and LPS groups, respectively. Service period was $149.6{\pm}34.3\;and\;53.0{\pm}12.5$ days for control and LPS groups, respectively(p<0.05). Services per conception was $3.6{\pm}0.8\;and\;2.1{\pm}0.3$ for control and LPS groups, respectively(p<0.05). In conclusion, intra-uterine infusion of LPS in cows with retained placenta can reduce the service period and services per conception. Reduced service period can improve the dairy farm profitability by decreasing cost un feeding and management of cows.