Browse > Article

Antimicrobial and Antioxidant Activity of the Discorea alata L.  

Kwon, Jeong-Eun (Dept. of Food and Nutrition, Andong National University)
Kwon, Jung-Bae (Experiment Station of Bioresources, Gyeongbuk Agricultural Research & Extension Services)
Kwun, In-Sook (Dept. of Food and Nutrition, Andong National University)
Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.3, 2010 , pp. 283-288 More about this Journal
Abstract
Yam (Dioscorea spp.) has been used as important edible and medicinal natural resource in worldwide and D. alata L. is most popular nourishment among the yam. In this study the composition, color, antioxidation and antimicrobial activity of D. alata Gyeongbuk No. 6 (GB-6), which was established in Gyeongbuk Agricultural Research & Extension Services, Andong, Korea, was compared to those of D. batatas Gyeongbuk No. 1 (GB-1), a major domestic cultivation species. Water content of GB-6 was $78.02{\pm}0.16%$, which is slightly lower than that of GB-1 ($82.6{\pm}0.07%$). The contents of crude protein, crude fat, crude fiber and ash of GB-6 were 0.95, 0.26, 0.85 and 0.70%, whereas those of GB-1 were 1.58, 0.15, 1.39 and 0.88%, respectively. Analysis of color using colormeter showed that the GB-6 is slight dark-yellow than GB-1, and total polyphenol content of GB-6 was 2-fold higher compared than that of GB-1. Sequential organic solvent fractions from methanol extract of GB-6 showed that the ethylacetate fraction has highest total polyphenol ($144.1{\pm}3.20\;mg/g$). Determination of antioxidation activity showed that the ethylacete fraction and water fraction has strong DPPH radical scavenging activity ($IC_{50}=78.32\;{\mu}g/mL$) and reducing power, respectively. In antimicrobial activity assay, the n-hexane and ethylacetate fraction showed antibacterial activity against B. subtilis, L.monocytogenes, S. epidermidis, S. aureus, P. vulgaris, and S. typhimurium. These results provide the possibility of domestic cultivated D. alata GB-6 as a healthy food.
Keywords
Dioscorea alata Gyeongbuk No. 6; total polyphenol; crude fat; antibacterial; DPPH radical scavenging; reducing power;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Cheng, W. Y., Y. H. Kuo, and C. J. Huang. 2007. Isolation and identification of novel estrogenic compounds in yam tuber (Dioscorea alata Cv. Tainung No. 2). J. Agric. Food Chem. 55: 7350-7358.   DOI   ScienceOn
2 Kum, E. J., S. J. Park, B. H. Lee, J. S. Kim, K. H. Son, and H. Y. Sohn. 2006. Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscroea batatas Decne. J. Life Sci. 16: 647-652.   과학기술학회마을   DOI
3 Liu, Y. H., Y. S. Lin, D. Z. Liu, C. H. Han, C. T. Chen, M. Fan, and W. C. Hou. 2009. Effects of different types of yam (Dioscorea alata) products on the blood pressure of spontaneously hypertensive rats. Biosci Biotechnol Biochem. 73: 1371-1376.   DOI   ScienceOn
4 Blanco-Metzler, A., J. Tovar, and M. Fernandez-Piedra. 2004. Nutritional characterization of carbohydrates and proximal composition of cooked tropical roots and tubers produced in Costa Rica. Arch. Latinoam. Nutr. 54: 322-327.
5 Chang, K. J., J. I. Park, S. L. Kim, J. H. Park, and B. J. Park. 2002. Studies on the growth and enlargement of tuber in tropical yams (Dioscorea alata L.). Kor. J. Plant Res. 15: 285-292.   과학기술학회마을
6 Chen, P. Y., Y. X. Tu, C. T. Wu, T. T. Jong, and C. M. Chang. 2004. Continuous hot pressurized solvent extraction of 1,1-diphenyl-2-picrylhydrazyl free radical scavenging compounds from Taiwan yams (Dioscorea alata). J. Agric. Food Chem. 52: 1945-1949.   DOI   ScienceOn
7 Fu, S. L., Y. H. Hsu, P. Y. Lee, W. C. Hou, L. C. Hung, C. H. Lin, C. M. Chen, and Y. J. Huang. 2006. Dioscorin isolated from Dioscorea alata activates TLR4-signaling pathways and induces cytokine expression in macrophages. Biochem. Biophys. Res. Commun. 339: 137-144.   DOI   ScienceOn
8 Hsu, F. L., Y. H. Lin, M. H. Lee, C. L. Lin, and W. C. Hou. 2002. Both dioscorin, the tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1), and its peptic hydrolysates exhibited angiotensin converting enzyme inhibitory activities. J. Agric. Food Chem. 50: 6109-6113.   DOI   ScienceOn
9 Imbert, M. P. and C. Seaforth. 1968. Anthocyanins in Dioscorea alata L. Experientia 24: 445-447.   DOI   ScienceOn
10 Kim, J. I., H. S. Jang, J. S. Kim, and H. Y. Sohn. 2009. Evaluation of antimicrobial, antithrombin, and antioxidant activity of Dioscorea batatas Decne. Kor. J. Microbiol. Biotechnol. 37: 133-139.   과학기술학회마을
11 Arnau, G., A. Nemorin, E. Maledon, and K. Abraham. 2009. Revision of ploidy status of Dioscorea alata L. (Dioscoreaceae) by cytogenetic and microsatellite segregation analysis. Theor. Appl. Genet. 118: 1239-1249.   DOI   ScienceOn
12 A.O.A.C. 1990. Official methods of analysis. 15th ed. Association of official analytical chemists. Washington D.C.
13 Aderiye, B. I., S. K. Ogundana, S. A. Adesanya, and M. F. Roberts. 1996. Antifungal properties of yam (Dioscorea alata) peel extract. Folia Microbiol. (Praha) 41: 407-412.   DOI   ScienceOn
14 Ahn, J. H., K. H. Son, H. Y. Sohn, and S. T. Kwon. 2005. In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Kor. J. Plant Biotechnol. 32: 317-323.   과학기술학회마을
15 Wanasundera, J. P. and G. Ravindran. 1994. Nutritional assessment of yam (Dioscorea alata) tubers. Plant Foods Hum. Nutr. 46: 33-39.   DOI   ScienceOn
16 Lawal, O. S., M. D. Lechner, and W. M. Kulicke. 2008. Single and multi-step carboxymethylation of water yam (Dioscorea alata) starch: synthesis and characterization. Int. J. Biol. Macromol. 42: 429-435.   DOI   ScienceOn
17 Lee, S. C., C. C. Tsai, J. C. Chen, J. G. Lin, C. C. Lin, M. L. Hu, and S. Lu. 2002. Effects of "Chinese yam" on hepatonephrotoxicity of acetaminophen in rats. Acta Pharmacol. Sin. 23: 503-508.
18 Lin, P. L., K. W. Lin, C. F. Weng, and K. C. Lin. 2009. Yam storage protein dioscorins from Dioscorea alata and Dioscorea japonica exhibit distinct immunomodulatory activities in mice. J. Agric. Food Chem. 57: 4606-4613.   DOI   ScienceOn
19 Liu, Y.W., H. F. Shang, C. K. Wang, F. L. Hsu, and W. C. Hou. 2009. Immunomodulatory activity of dioscorin, the storage protein of yam (Dioscorea alata cv. Tainong No. 1) tuber. Food Chem. Toxicol. 45: 2312-2318.
20 Sohn, H. Y. H. Y. Ryu, Y. J. Jang, H. S. Jang, Y. M. Park, and S. Y. Kim. 2008. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Kor. J. Microbiol. Biotechnol. 36: 195-200.   과학기술학회마을
21 Wang, T. S., S. J. Liang, C. K. Lii, and S. Y. Liu. 2004. Protective effect of water yam (Dioscorea alata L.) extract on the copper-driven fenton reaction and X-ray induced DNA damage in vitro. Phytother. Res. 18: 325-328.   DOI   ScienceOn
22 Wu, Z., C. Leng, Z. Tao, Y. Wei, and C. Jiang. 2009. Genetic diversity of Dioscorea alata based on ISSR analysis. Zhongguo Zhong. Yao Za Zhi. 34: 3017-3020.
23 Yang, M. H., K. D. Yoon, Y. W. Chin, and J. W. Kim. 2009. Phytochemical and pharmacological profiles of Dioscorea species in Korea, China and Japan. Kor. J. Pharmacogn. 40: 257-279.   과학기술학회마을