• Parasites, Hosts and Diseases
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• 제53권2호
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• pp.147-154
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• 2015

Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it should be properly monitored by an inhibition control. In this study, a cloning vector harboring a modified target DNA sequence (${\approx}375bp$) was constructed to be used as a competitive internal amplification control (IAC) for a conventional PCR assay that detects ${\approx}550bp$ of the Cryptosporidium oocyst wall protein (COWP) gene sequence in human feces. Modification of the native PCR target was carried out using a new approach comprising inverse PCR and restriction digestion techniques. IAC was included in the assay, with the estimated optimum concentration of 1 fg per reaction, as duplex PCR. When applied on fecal samples spiked with variable oocysts counts, ${\approx}2$ oocysts were theoretically enough for detection. When applied on 25 Cryptosporidium-positive fecal samples of various infection intensities, both targets were clearly detected with minimal competition noticed in 2-3 samples. Importantly, both the analytical and the diagnostic sensitivities of the PCR assay were not altered with integration of IAC into the reactions. When tried on 180 randomly collected fecal samples, 159 were Cryptosporidium-negatives. Although the native target DNA was absent, the IAC amplicon was obviously detected on gel of all the Cryptosporidium-negative samples. These results imply that running of the diagnostic PCR, inspired with the previously developed DNA extraction protocol and the constructed IAC, represents a useful tool for Cryptosporidium detection in human feces.

• 한국환경과학회지
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• 제13권2호
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• pp.149-159
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• 2004

In the removal of heavy metals from the mine deposit using electrokinetic processes, the effects of operation under both constant current and constant potential conditions were estimated. The results of soil pH distributions for DDW-20 V and DDW-100 mA cases after the electrokinetic remediation tests were observed. In the former case, soil pH was not much changed and kept to almost constant value just little higher than initial soil pH of 3.52, except near the cathode, which was about pH 5. While in the latter case, soil pHs of anode and the cathode regions were less than pH 3 and about 6, respectively. The electroosmotic flow to the cathode increased rapidly till 10 hrs and decreased steadily and then maintained to constant rate until the end of operation at constant current condition. Electric potential gradient was continuously increased to as much as 34.375 V/cm. At the steady state, values of the apparent electric conductivity for DDW-20 V and DDW-100 mA were around 40 ${\mu}\textrm{s}$/cm and 30 ${\mu}\textrm{s}$/cm, respectively. In the DDW-100mA test, Cu, Cd, and Zn except Pb showed the tendency of moving toward the cathode. While in the DDW-20 V case, it was observed that Cu, Zn, and Pb except Cd were not moved to any directions. The results of the tests demonstrated that the electrokinetic soil remediation process could be operated better under constant current condition than constant electric potential condition.

• 간호행정학회지
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• 제7권2호
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• pp.361-375
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• 2001

This research aims to produce and implement web-based continuing nurse education programs in response to nurses' educational needs, and to verify them, thus preparing for the times that the program will be offered via web. This research designed, produced and implemented 'emergency nursing' and 'medical fee schedule management' subjects based on Jung, In-seong's(1997) web-based instructional system design, and then compared the learning achievements of web-based learning group of 38 people with those of face-to-face learning group of 39 people. The questionnaire have been developed by these researchers to measure pre-learning knowledge on 'emergency nursing' and 'medical fee schedule management.' Data collected for this research have been given statistical analysis, using SPSS 10.0 for Windows Program. As a result of giving Mann-Whitney test, with respect to pre-learning prior knowledge level, there was no significant difference between the web-based learning group and the face-to-face learning group(Z=-.092, p=.926), while after completing learning, there was a significant difference in the learning achievements between the web-based learning group and the face-to-face learning group(Z=-2.406, p=.008). That is, this research revealed this: the web-based learning group and the face-to face learning group with both having no significant difference in the pre-learning level, after receiving the continuing education each with different methods(face-to-face education and web-based education), showed that the web-based learning groups attained higher learning achievements than the face-to-face learning groups. This result proves the effect of the web-based education to be no worse or even better than that of the face-to-face education, provided that choices of appropriate themes and quality courses composition, as well as systematic design development effective implementation are guaranteed.

• Mycobiology
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• 제42권4호
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• pp.368-375
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• 2014

Red ginseng (Panax ginseng), a Korean traditional medicinal plant, contains a variety of ginsenosides as major functional components. It is necessary to remove sugar moieties from the major ginsenosides, which have a lower absorption rate into the intestine, to obtain the aglycone form. To screen for microorganisms showing bioconversion activity for ginsenosides from red ginseng, 50 yeast strains were isolated from Korean traditional meju (a starter culture made with soybean and wheat flour for the fermentation of soybean paste). Twenty strains in which a black zone formed around the colony on esculin-yeast malt agar plates were screened first, and among them 5 strains having high ${\beta}$-glucosidase activity on p-nitrophenyl-${\beta}$-D-glucopyranoside as a substrate were then selected. Strain JNO301 was finally chosen as a bioconverting strain in this study on the basis of its high bioconversion activity for red ginseng extract as determined by thin-layer chromatography (TLC) analysis. The selected bioconversion strain was identified as Candida allociferrii JNO301 based on the nucleotide sequence analysis of the 18S rRNA gene. The optimum temperature and pH for the cell growth were $20{\sim}30^{\circ}C$ and pH 5~8, respectively. TLC analysis confirmed that C. allociferrii JNO301 converted ginsenoside Rb1 into Rd and then into F2, Rb2 into compound O, Rc into compound Mc1, and Rf into Rh1. Quantitative analysis using high-performance liquid chromatography showed that bioconversion of red ginseng extract resulted in an increase of 2.73, 3.32, 33.87, 16, and 5.48 fold in the concentration of Rd, F2, compound O, compound Mc1, and Rh1, respectively.

• Animal Systematics, Evolution and Diversity
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• 제34권1호
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• pp.37-49
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• 2018

Two hypotrich ciliates, Australocirrus shii (Shi et al., 1997) Kumar & Foissner, 2015 and Afrokeronopsis aurea (Foissner & Stoeck, 2008) Foissner et al., 2010 isolated from freshwater habitats in Korea and were studied based on the specimens from live and after protargol impregnation. Australocirrus shii is redescribed based on morphology and 18S rRNA gene sequence, whereas Af. aurea is the first record for Korea. Main morphological features of the Korean population of Au. shii are as following: body size $100-200{\times}40-80{\mu}m$ in vivo; elongate to ellipsoidal or slightly elongate obovate, dorsoventrally flattened; transverse cirri arranged in (3+2) pattern, anterior pretransverse ventral cirrus distantly anterior of the first transverse cirrus; eight or nine dorsal kineties; and three caudal cirri. Main morphological features of the Korean population of Af. aurea are as following: body size $230-375{\times}70-145{\mu}m$ in vivo; shape elongate obovate or ellipsoidal, widest at the mid-body; undulating membranes in Australocirrus pattern with a buccal depression; and three caudal cirri. The Korean population of A. shii is similar in morphology with previous descriptions except for the presence of indentation at the posterior end in the Korean population. The Korean population of A. aurea is slightly shorter than the South African population and has slightly less marginal and mid-ventral cirri. The phylogenetic analysis of present two Korean hypotrichs and relevant species based on 18S rRNA gene sequences generated almost similar tree topologies compared with previous studies.

• 한국의류학회지
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• 제31권3호
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• pp.364-375
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• 2007

The propose of this study is to develop the optimal sizing system of ready-to-wear far elementary school boys using a newly invented statistical technique. The body measurements was classified by the method that equalizes the distribution of the subjects using the probability density function, to theoretically systemize a method to determine a size range of ready-to-wear for elementary school boys between 7 to 12 yeiws old. The results were as follows: 1. Height group includes 9 types of heights: 115, 120, 125, 130, 135, 140, 145, 150 and 155. 2. In the case of short children's groups, the variance in bust girth and waist girth is narrow. The people cluster together around the average. The size deviation of ready-to-wear is small. 3. In the case of tall children's groups, the variance in bust girth and waist girth is wide. The people spread widely around the average. The size deviation of ready-to-wear is large. 4. The optimal size system is suggested considering the weight of growth exponent of children according to their respective ages. Clothing companies can selectively choose sizes that meet the target of their brands. 5. It suggests the body sizes chart, which based on their means by the middle size children for each height group, so that clothing companies make use of it.

• 한국CDE학회논문집
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• 제10권5호
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• pp.375-379
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• 2005

Durability of rubber dust cover in the ball joint for automotive suspension parts is analyzed by FEM and compared with experimental data. Upper open area of ball joint is sealed by dust cover for preventing outflow of the lubricating oil and intrusion of send, dust, water, etc. This rubber cover undergoes repeated loadings such as tension and compression while the car is running. Analysis about rubber material needs to consider every kinds of nonlinearities arise in finite element analysis, which are geometric nonlinearity due to large displacement and small strain, materially nonlinearity and nonlinear boundary condition such as contact. The deformation behavior of dust cover is analysed by using the commercial finite element program MARC. In the study, this program could solve these kinds of nonlinear analysis accurately. Finite element model of dust cover is considered as 3-dimensional half model based on 2-dimensional axisymmetric model. Material property of rubber is modeled by Ogden model and input data for calculation takes form uniaxial tension test of rubber specimen. The final object of the study is obtaining the design specification of dust covers and the result of analysis should be a useful data to design of rubber cover.

• Asian-Australasian Journal of Animal Sciences
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• 제25권3호
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• pp.375-381
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• 2012

Two experiments were conducted to establish a digestible energy (DE) content prediction model of rapeseed meal for growing-finishing pig based on rapeseed meal's chemical composition. In experiment 1, observed linear relationships between the determined DE content of 22 rapeseed meal calibration samples and proximate nutrients, gross energy (GE) and neutral detergent fiber (NDF) were used to develop the DE prediction model. In experiment 2, 4 samples of rapeseed meal selected at random from the primary rapeseed growing regions of China were used for testing the accuracy of DE prediction models. The results indicated that the DE was negatively correlated with NDF (r = -0.86) and acid detergent fiber (ADF) (r = -0.73) contents, and moderately correlated with gross energy (GE; r = 0.56) content in rapeseed meal calibration samples. In contrast, no significant correlations were found for crude protein, ether extract, crude fiber and ash contents. According to the regression analysis, NDF or both NDF and GE were found to be useful for the DE prediction models. Two prediction models: DE = 16.775-0.147${\times}$NDF ($R^2$ = 0.73) and DE = 11.848-0.131${\times}$NDF+0.231${\times}$GE ($R^2$ = 0.76) were obtained. The maximum absolute difference between the in vivo DE determinations and the predicted DE values was 0.62 MJ/kg and the relative difference was 5.21%. Therefore, it was concluded that, for growing-finishing pigs, these two prediction models could be used to predict the DE content of rapeseed meal with acceptable accuracy.

• 대한지리학회지
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• 제45권3호
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• pp.375-389
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• 2010

한라산 표고 1,710m에 발달한 나지 표층에 2개의 페인트 라인을 설치하여 자갈의 이동량을 관측하고, 이동 프로세스와 관련된 요인을 검토하였다. 관측 기간에 발생한 자갈의 이동량은 평균 58.2cm로서 일평균 이동 거리는 0.24cm이다. 계절별로 비교하면 동결기 0.3cm에 대하여 비동결기는 0.05cm에 불과하여 표층 자갈은 주로 동결기에 주빙하 프로세스를 통하여 이동하고 있다. 특히 서릿발 포행을 비롯한 동상 포행이 자갈의 이동을 주도하고 있다. 사면 경사각의 영향은 관측 기간 내내 측선 II의 이동량이 측선 I보다 큰 결과에 반영되어 있지만, 암괴와 식생이 자갈의 이동을 방해하므로 절대적이지는 않다. 자갈의 크기와 비중도 이동량에 관여하는 요인으로서 특히 스코리아의 작은 비중이 매우 큰 이동량을 가져왔다.

• 지역사회간호학회지
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• 제13권2호
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• pp.363-375
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• 2002

Objectives: The aim of this study was to analyze the trend of research on cancer prevention and early detection in Korea, in order to suggest a future direction of research on cancer prevention and early detection for Koreans. Methods: A total of 97 studies published from 1980 to 2001 were analyzed according to the year of publication, research design, journal type, cancer type, major study concepts, and findings. Results: 1) The number of studies related to cancer prevention and early detection had increased rapidly since the year 1995. 2) The most frequently used research design in the studies was the descriptive study design (55.7%). 3) There were 10 master's theses on cancer prevention and early detection, and 10 studies published in the Korean Epidemiology Journal. 4) When classified by the published field, 47 studies (48.5%) were published in nursing journals, 46 studies (47.4%) were published in medical journals, and 4 studies (4.1%) were published in public health journals. 5) The major topics of the studies were cancer prevention (51.5%), early detection (44.4%), and cancer prevention and early detection (4.1%). 6) Breast cancer was the most largely addressed issue in the studies (N=25; 25.7%), followed by lung cancer (N=23; 23.7%), hepatoma (N=17; 17.5%), gastric cancer (N=16; 16.5%), other general type of cancer (N=6; 6.2%), colorectal cancer (N=5; 5.2%) and cervical cancer (N=5; 5.2%). Conclusion: It is suggested that there should be more studies on cancer prevention and early detection in the future, and, particularly, experimental studies to exam the effects of intervention on cancer prevention and early detection are considered necessary.