• Title/Summary/Keyword: A375-S2

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Effect of Application Rate of Hairy Vetch on Ammonia Emission from Paddy Soil (논에서 헤어리베치 시용량에 따른 암모니아 휘산량 평가)

  • Kim, TaeYoung;Daquiado, Aileen Rose;Alam, Faridul;Lee, YongBok
    • Korean Journal of Environmental Agriculture
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    • v.31 no.4
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    • pp.375-377
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    • 2012
  • BACKGROUND: Hairy ventch (Vicia villosa) is a good green manure for supplying nitrogen in arable soil. Ammonia emission from rice fields can occur, and the degree of this emission can be great. However, quantitative information of ammonia emission from paddy soil using green manure is required to obtain emission factors for rice cropping in Korea. METHODS AND RESULTS: Ammonia emission from flooding soil with different application rate of hairy vetch was measured using the closed chamber method. For this study, hairy vetch was applied at rates of 0 (control), 500 (H500), 1000 (H1000), 2000 (H2000), and 3000 (H3000) kg/ha (fresh matter basis). This experiment was conducted for 54 days under flooding condition. The total NH3 emission throughout the experiment period was 0.32, 0.54, 1.20, 4.20, and 6.20 kg/ha for control, H500, H1000, H2000, and H3000, respectively. The ratio of NH3 emission to applied nitrogen by hairy vetch for each treatment was 0.7, 1.4, 3.2, and 3.2% for H500, H1000, H2000, and H3000, respectively. CONCLUSION(S): A very small amount of ammonia emission was recorded in the present study. Therefore, the use of hairy vetch in paddy field instead of chemical fertilizer can reduce ammonia emissions.

Blended Learning Applied Curriculum Design for Nursing Department's Computer-Utilizing Academic Subjects (간호학과의 컴퓨터 활용 교과목 수업을 위한 브랜디드 러닝을 적용한 교과과정 설계)

  • Yoon, Sung-Ja;Kim, No-Whan;Park, Jin-Seob
    • The Journal of the Korea institute of electronic communication sciences
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    • v.12 no.2
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    • pp.375-384
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    • 2017
  • This paper is intended to provide a good lecture to computer utilizing courses in the Department of Nursing, therefore first analyzes the outlines of relevant qualifying examinations as well as the contents of the textbooks and syllabus that are currently taught in universities, and then design the curriculum by applying blended learning for effective proceedings of computer-utilizing course. The curriculum for computer-utilizing course which this paper suggests is based on blended learning which blends face-to-face classes with e-learning classes, and its two tracks of teaching and practice include weekly core areas, teaching goals, and subjects. Therefore, this curriculum is expected to lead to excellent learning outcomes as it will become a good teaching scheme for teachers and will motivate students to acquire license. and to find employment.

Reliability and Validity of the Korean Version of the Coping and Adaptation Processing Scale-Short-Form in Cancer Patients

  • Song, Chi Eun;Kim, Hye Young;So, Hyang Sook;Kim, Hyun Kyung
    • Journal of Korean Academy of Nursing
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    • v.48 no.3
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    • pp.375-388
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    • 2018
  • Purpose: This study was conducted to assess the reliability and validity of the Korean version of the Coping and Adaptation Processing Scale-Short- Form in patients with cancer. Methods: The original scale was translated into Korean using Brislin's translation model. The Korean Short-Form and the Functional Assessment Cancer Therapy-General were administered to 164 Korean patients with cancer using convenience sampling method. The collected data were analyzed using SPSS 23.0 and AMOS 23.0. Construct validity, criterion validity, test-retest reliability, and internal consistency reliability of the Korean Coping and Adaptation Processing Scale-Short-Form were evaluated. Results: Exploratory factor analysis supported the construct validity with a four-factor solution that explained 60.6% of the total variance. Factor loadings of the 15 items on the four subscales ranged .52~.86. The four-subscale model was validated by confirmatory factor analysis (Normed ${\chi}^2=1.38$ (p=.013), GFI=.92, SRMR=.02, RMSEA=.05, TLI=.94, and CFI=.95), and criterion validity was demonstrated with the Functional Assessment Cancer Therapy-General. Cronbach's alpha for internal consistency of the total scale was .83 and ranged .68~.81 for all subscales, demonstrating sufficient test-retest reliability. Conclusion: The Korean version showed satisfactory construct and criterion validity, as well as internal consistency and test-retest reliability.

Regulation of Branched-Chain, and Sulfur-Containing Amino Acid Metabolism by Glutathione during Ultradian Metabolic Oscillation of Saccharomyces cerevisiae

  • Sohn Ho- Yong;Kum Eun-Joo;Kwon Gi-Seok;Jin Ingnyol;Kuriyama Hiroshi
    • Journal of Microbiology
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    • v.43 no.4
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    • pp.375-380
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    • 2005
  • Autonomous ultradian metabolic oscillation (T$\simeq$50 min) was detected in an aerobic chemostat culture of Saccharomyces cerevisiae. A pulse injection of GSH (a reduced form of glutathione) into the culture induced a perturbation in metabolic oscillation, with respiratory inhibition caused by $H_2S$ burst pro-duction. As the production of $H_2S$ in the culture was controlled by different amino acids, we attempted to characterize the effects of GSH on amino acid metabolism, particularly with regard to branched chain and sulfur-containing amino acids. During stable metabolic oscillation, concentrations of intra-cellular glutamate, aspartate, threonine, valine, leucine, isoleucine, and cysteine were observed to oscil-late with the same periods of dissolved $O_2$ oscillation, although the oscillation amplitudes and maximal phases were shown to differ. The methionine concentration was stably maintained at 0.05 mM. When GSH (100 $\mu$M) was injected into the culture, cellular levels of branched chain amino acids increased dramatically with continuous $H_2S$production, whereas the cysteine and methionine concentrations were noticeably reduced. These results indicate that GSH-dependent perturbation occurs as the result of the promotion of branched chain amino acid synthesis and an attenuation of cysteine and methionine synthesis, both of which activate the generation of $H_2S$. In a low sulfate medium containing 2.5 mM sulfate, the GSH injections did not result in perturbations of dissolved $O_2$ NAD(P)H redox oscillations without burst $H_2S$ production. This suggests that GSH-dependent perturbation is intimately linked with the metabolism of branched-chain amino acids and $H_2S$ generation, rather than with direct GSH-GSSG redox control.

Expression of CP4 5-Enol-Pyruvylshikimate-3- Phosphate Synthase Transgene in Inbred Line of Korean Domestic Maize (Zea may L.) (국내 옥수수 순계주에서 CP4 5-Enol- Pyruvylshikimate-3- Phosphate Synthase 유전자의 발현)

  • Cho, Mi-Ae;Kwon, Suk-Yoon;Kim, Jin-Seog;Lee, Byoung-Kyu;Moon, Choo-Yeun;Choi, Pil-Son
    • Journal of Plant Biotechnology
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    • v.34 no.4
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    • pp.375-380
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    • 2007
  • This study was conducted to develop herbicide-resistance domestic maize plants by introducing the CP4 5-enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) gene using Agrobacterium tumefaciens-mediated immature embryo transformation. Immature embryos of five genotypes (HW1, KL103, HW3, HW4, HW7) were co-cultivated with strains Agrobacterium tumefaciens (strain C58C1) containing the binary vector (pCAMBIA2300) carrying Ubiquitin promoter-CP4 EPSPS gene and Cauliflower mosaic virus 35S (CaMV35S) promoter-nptll gene conferring resistance to paromomycin as a selective agent. The presence and expression of CP4 EPSPS transgene were confirmed by PCR, RT-PCR and Northern blot analysis, respectively. Also, the resistance to glyphosate in the transgenic maize ($T_1$) was analyzed by shikimate accumulation assay. The frequency (%) of paromomycin-resistance callus was 0.37, 0.03, 2.20, 2.37, and 0.81% in pure lines HW1, KL103, HW3, HW4 and HW7, respectively. EPSP transgene sequences were amplified in putative transgenic plants that regenerated from paromomycin-resistance calli of two inbred lines (HW3, HW4). Of them, RT-PCR and Northern blot analyses revealed that the transgene was only expressed in two transgenic events (M266, M104) of HW4 inbred line, and a mild glyphosate resistance of transgenic event (M266) was confirmed by the lower shikimate accumulation in leaf segments. These results demonstrate that transgenic maize with herbicide-resistance traits in Korean genotype can be genetically obtained.

A Study on the Design and Implementation of CDMA Modem using DSP (DSP를 이용한 CDMA 모뎀 설계 및 구현에 관한 연구)

  • Park, Jin-Hong;Kang, Byeong-Gwon
    • Proceedings of the KIEE Conference
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    • 2001.11c
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    • pp.372-375
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    • 2001
  • 본 논문에서는 고속데이터 전송을 위한 CDMA 모뎀를 구현하였다. 데이터율 1Mbps의 트래픽 5채널에 직교부호를 곱하여 채널을 구분한 후 하나의 채널로 처리하였다. I,Q로 입력된 신호는 복소 곱셈기에서 칩 레이트 8Mcps로 OCQPSK(또는 HPSK) 변조하였다. 복조기는 I,Q의 신호를 역확산한 후 직교부호를 다시 곱하여 각 채널의 데이터를 분리한다. 변복조기의 구현은 클럭 속도 167MHz의 부동 소수점형 프로세서인 TI사의 TMS320C6701 DSP(Digital Signal Processor)를 사용하었고, long code 및 I,Q 채널 PN 코드는 IMT-2000 동기방식과 비동기방식의 규격에 정의된 2가지의 PN코드 발생기를 모두 구현하였다.

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A Comparative Study of Dissatisfaction towards Imported and Domestic Clothing (수입의류와 국산의류의 불만족에 관한 비교연구)

  • 박혜정;박재옥
    • Journal of the Korean Society of Clothing and Textiles
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    • v.26 no.2
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    • pp.364-375
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    • 2002
  • The purpose of this study is to identify the differences between married women's dissatisfaction towards imported and domestic clothing and to test the role of socioeconomic variables on dissatisfaction. Two-piece formal suit was selected as the item, and country of origin of imported suit was not controlled. Sample selection criteria included married women living in Seoul, Korea, between ages 30-59. The data were analysed using factor analysis, paired t-test, and one-way ANOVA. The results indicate, in the Korean clothing market, both international and Korean marketers should be prudent with their price policies and should implement differentiated marketing strategy based on their target market's demogrophic profile. Higher dissatisfaction towards domestic clothing in terms of most factors, under the rapidly globalizing market environment suggests the Korean marketers will be forced to face full-scale harsh competition from international marketers.

Deep digital flexor tendonectomy in cats

  • Yeon, Seong-chan
    • Korean Journal of Veterinary Research
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    • v.39 no.2
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    • pp.370-375
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    • 1999
  • Owner's attitudes for tendonectomy, the advantages of this surgical technique, and postoperative complication were investigated by telephone survey. 18 cats on whom tendoncetomy was performed with or without concurrent ovario-hysterectomy or castration were included in this study. The first reason for tendonectomy was to avoid damage caused by the cat's scratching household materials. The first benefit of tendonectomy was decreasing damage to materials (89%). The primary concern of the owners of cats that underwent tendonectomy was postoperative pain after surgery (61%). Twelve cats (67%) that underwent tendonectomy recovered fully within the first three days and 6 cats (33%) recovered within two weeks. After combining the very positive rating and positive as positive, seventeen owners (94%) of cats that underwent tendonectomy had a positive attitude to the surgery.

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The production of monoclonal antibodies against canine distemper virus (Canine Distemper Virus에 대한 단클론성 항체의 생산)

  • Kim, Tae-jong;Kim, Se-young
    • Korean Journal of Veterinary Research
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    • v.37 no.2
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    • pp.375-381
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    • 1997
  • The purpose of the production of monoclonal antibodies aganist the Canine distemper virus(CDV) were perfect diagnosis and a new approach to treat canine distemper because the diagnosis and treatment of canine distemper were difficult. Canine distemper virus(CDV) was purified using saturated ammonium sulfate, and injected into hind footpads of BALB/c mouse. 12-15 days later, popliteal lymph node(PN) cells were harvested and fused with SP2/O myeloma cells. Characteristics of monoclonal antibodies were analysed. 1. 9 hybridomas produce the specific antibody against CDV. 2. 6 monoclonal antibodies are against intranuclear and cytoplasmic component of CDV, and 3 monoclonal antibodies are against cytoplasmic inclusions. 3. All monoclonal antibodies did not react with other 5 different viruses (CAV-I, CAV-II, CCV, CPV and CPIV) and react with another CDV-FXNO strain. 4. 3 monoclonal antibodies have neutralizing activity against CDV. 5. Antigenic difference was observed between CDV by IFA.

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In vitro Effects of Essential Oils from the Aerial Parts of Artemisia annua L. Against Antibiotic-Susceptible and -Resistant Strains of Salmenella typhimurium (항생제 내성 및 감수성 Salmonella typhimurium 균주에 대한 개똥쑥 지상부 정유와 Kanamycin의 병용효과)

  • Shin, Seung-Won
    • YAKHAK HOEJI
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    • v.51 no.5
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    • pp.355-360
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    • 2007
  • The essential oil fraction from the aerial parts of A. annua was analyzed by GC-MS. As the results, caryophyllene oxide (11.7%), caryophyllene (7.54%), camphor (7.32%), 1,8-cineol (4.98%), and borneol (3.99%) were confirmed as the main components of the oil fraction. The effects of this oil and its main components on antibiotic-susceptible and -resistant strains of Salmonella enteritidis and S. typhimurium were assessed. A. annua oil fraction significantly inhibited all strains of the two Salmonella species examined, with minimum inhibiting concentrations (MICs) ranging from 2.0 mg/ml to 8.0 mg/ml. Among the main components of the oil, borneol and camphor showed relatively strong inhibiting activity with MICs between 1.0 mg/ml and 4.0 mg/ml. The MICs of caryophyllene and caryophyllene oxide were higher than 16 mg/ml. The combination effects of the oils with kanamycin were evaluated using a checkerboard microtiter assay. Against S. typhimurium KCCM11862 and CCARM8009 strains, the oil fraction of A. annua, camphor, and 1,8-cineol exhibited significant synergistic with kanamycin with fractional inhibitory concentration (FIC) indices in the range of 0.085 to 0.375. In conclusion, a combination of kanamycin and A. annua oil or its main component, camphor, and cineol, may be useful for reducing the minimum effective dose of antibiotic required for the treatment of resistant S. typhimurium infections.