• 아시안잔디학회지
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• 제24권2호
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• pp.165-169
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• 2010

Fusarium circinatum은 소나무류에 푸사리움 가지마름병을 유발하는 병원균으로 알려져 있다. 본 연구에서는 골프장의 리기다소나무로부터 병원균을 분리하였으며, 이들의 형태적인 특성을 관찰하고 분자생물학적인 방법을 이용하여 동정한 결과 F. circinatum 임을 확인하였다. 특히 histone H3 유전자와 IGS rDNA의 염기서열을 분석한 결과 분리된 균들의 염기서열은 동일하였으며, 유성생식(mating)에 관여하는 유전자를 분석한 결과 모두 MAT1-1 유형임을 확인하였다. 또한 푸사리움 가지마름병에 대한 감수성 수종인 리기다소나무를 비롯하여 저항성 수종인 잣나무와 소나무의 메탄올 추출물을 만들어 이들의 병원균에 대한 항균력을 검정하였다. 그 결과 저항성 수종인 잣나무와 소나무의 추출물에서 리기다소나무 보다 높은 항균력을 확인 하였다.

• The Plant Pathology Journal
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• 제27권1호
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• pp.20-25
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• 2011

Gibberella zeae (anamorph: Fusarium graminearum), a homothallic (self-ferile) ascomycete with ubiquitous geographic distribution, causes serious diseases in several cereal crops. Ascospores (sexual spores) produced by this fungal pathogen have been suggested as the main source of primary inoculum in disease development. Here, we report the function of a gene designated GzRUM1, which is essential for ascospore formation in G. zeae. The deduced product of GzRUM1 showed significant similarities to the human retinoblastoma (tumor suppressor) binding protein 2 and a transcriptional repressor, Rum1 in the corn smut fungus (Ustilago maydis). The transcript of GzRUM1 was detected during the both vegetative and sexual stages, but was more highly accumulated during the latter stage. In addition, no GzRUM1 transcript was detected in a G. zeae strain lacking a mating-type gene (MAT1-2), a master regulator for sexual development in G. zeae. Targeted deletion of GzRUM1 caused no dramatic changes in several traits except ascospore formation. The ${\Delta}$GzRUM1 strain produced perithecia (sexual fruit bodies) but not asci nor ascospores within them. This specific defect leading to an arrest in ascospore development suggests that GzRUM1, as Rum1 in U. maydis, functions as a transcriptional regulator during sexual reproduction in G. zeae.

• 한국임상수의학회지
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• 제7권2호
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• pp.521-525
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• 1990

Microspoum canis에 감염된 개 및 고양이와 직접 접촉한 경험이 있는 5명의 환자를 조사하여 동물기생체로서의 이 진균의 중요성을 확인하였다. ring worm의 전형적인 병변은 신체의 노출된 부위에만 발생하였다. M. canis는 환자와 감염된 애완동물의 피부병변에서 분리되었으며 피부 시료로부터 직접 증명되었다. 그림에도 불구하고 비접촉군과 사물성환경으로 부터는 증명되지 않았다. 사람, 개 및 고양이에서 분리된 진균들은 육안적으로나 현미경적으로 서로 비슷한 형태를 나타내었다. 이 진균들의 유전학적 연구결과는 교배(-)형이었다. 동물기호성 표재성진균의 전염은 직접적인 접촉에 의해 자주 일어나는 것으로 사료된다. 이감염증에 대한 역학과 예방방법에 대해 고찰하였다.

• 한국식물병리학회지
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• 제14권2호
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• pp.115-119
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• 1998

Root rot of carnation caused by isolates of Phytophthora was found at Kongju, 1996. Infected plants appeared symptoms of wilting, brown discoloration on collar area and consequently led to death. The causal pathogen was identified as Phytophthora nicotianae based on morphological, cultural and physiological characteristics. Mycelium was grown to rosette colony pattern on corn meal agar and the growth was 10.2 mm/day on 10% clarified V8 medium at $25^{\circ}C$. Swelling with radiating hyphae was formed in water and on agar within 7 days. Chlamydospores were abundantly produced on agar within 7 days. Sporangium was prominent papillate, 2~3 apex, 1.2 : 1 l/b ratio, lateral attacment on sporangiospore and was rarely produced on solid media but produced in water. The shape of sporangium was spherical or ovoid, and the size was 34~73$\times$32~60 (av. 33$\times$66.5) ${\mu}{\textrm}{m}$ in dimension. The isolates were heterothallic, and mating type A2. Oogonium was spherical, ovoid, 26~36 (av. 36 ${\mu}{\textrm}{m}$) in diameter, and antheridium was amphygynious to oogonia. The fungus was able to grow 10~35$^{\circ}C$, and optimal temperature was 27$^{\circ}C$.

• 한국가축번식학회지
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• 제14권3호
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• pp.191-197
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• 1990

The transgenic mice were produced by microinjection of human growth hormone gene fused with mouse metallothionein Ⅰ promoter. They were mated with momal mice by backcross or brother-sister mating. The reproduction efficiencies of female and male n the FO transgenic mice were 17.6%(3/17 mice) and 31.2%(5/16 mice), respectively, and were very lower than that in normal mice(85.7% and 100%, respectively). Interestingly, a few of female transgenic mice were fertile which was different from the previous reports. Of 6 fertile transgenic mice, 2 mice were identified as mosaic type by the reduced frequency of genetic transmission to successive generation below Mendelian levle and the enhanced copy numbers of transgene in progeny mice compared with the transgenic parent. In the group of F1, F2, F3 transgenic mice the reproduction efficiencies of males were gradually improved, whereas females were absolutely infertile. It was consequently shown that the transgenic mice expressing human growth hormone gene were frequently infertile, but the genotypic and phenotypic characteristics of the fertile transgenic mice were normally passed on to the progeny through herm line. Therefore it must be considered wheter or not the products of foreign DNA introduced into animals will detrimentally affect their physiological aspects.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1536-1544
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• 2013

A least squares regression interval mapping model was derived to detect quantitative trait loci (QTL) with a unique mode of genomic imprinting, polar overdominance (POD), under a breed cross design model in outbred mammals. Tests to differentiate POD QTL from Mendelian, paternal or maternal expression QTL were also developed. To evaluate the power of the POD models and to determine the ability to differentiate POD from non-POD QTL, phenotypic data, marker data and a biallelic QTL were simulated on 512 F2 offspring. When tests for Mendelian versus parent-of-origin expression were performed, most POD QTL were classified as partially imprinted QTL. The application of the series of POD tests showed that more than 90% and 80% of medium and small POD QTL were declared as POD type. However, when breed-origin alleles were segregating in the grand parental breeds, the proportion of declared POD QTL decreased, which was more pronounced in a mating design with a small number of parents ($F_0$ and $F_1$). Non-POD QTL, i.e. with Mendelian or parent-of-origin expression (complete imprinting) inheritance, were well classified (>90%) as non-POD QTL, except for QTL with small effects and paternal or maternal expression in the design with a small number of parents, for which spurious POD QTL were declared.

• 한국버섯학회지
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• 제11권3호
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• pp.149-153
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• 2013

병재배용 고품질 장기저장성 느타리 품종인 '곤지7호'의 주요특성을 요약하면 다음과 같다. 균사생장적온은 $26{\sim}29^{\circ}C$이고 버섯발생 및 생육온도는 $16{\sim}18^{\circ}C$로 중온성을 나타내었다. 병재배시 배양기간은 30일, 초발이 소요일수는 4일, 생육일수는 4일로 총재배기간은 38일이 소요되었다. 갓크기는 46.6 mm이며, 갓색은 진회색을 나타냈다. 대직경 12.4 mm, 대길이 84.8 mm로 굵고 긴형태이며, 대색은 백색을 나타내었다. 수량은 생산력검정시 900 ml병에서 166 g을 나타내었으며, 농가실증재배시 농가1에서는 187 g/ 1100 ml, 농가2에서는 197 g/1100 ml으로 대조품종과 대등하였다. 대의 물리성에서 있어, 탄력성 83%, 응집성 71%, 씹읍성 352 g, 깨집성 29 kg으로 '춘추2호'와 '수한1호'의 중간특성을 나타내었다. Minamide법에 준한 저장성은 35일까지 신선도 등급 6 (판매가능 등급) 으로 대조품종에 비하여 7일정도 저장기간이 연장되었다. DNA다형성을 비교 분석한 결과, UFPF3, UFPF5, UFPF7등의 primer에서 교배모본인 '농민59호'와 'MT07156'의 DNA의 밴드가 혼합되어 있었으며 품종간, 균주간의 밴드 차이가 있었다.

• The Plant Pathology Journal
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• 제16권3호
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• pp.142-146
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• 2000

Ordered differential display using RT-PCR (ODD-PCR) was conducted to have a profile of the differently expressed genes between a hypovirulent strain of Cryphonectria parasitica (UEP1) and its isogenic wild type strain (EP155/2). ODD-PCR has advantages of high sensitivity, reproducibility, proportional representation, and limited number of primer combinations comparing with other differential display methods. RNAs were prepared from 1 and 5 day liquid culture of both hypovirulent and wild type strains, and were further evaluated with the marker genes of C. parasitica such as cryparin and mating factor MF2-1, which were already proven to be specifically down-regulated by the presence of mycovirus CHV1-713. ODD-PCR was conducted using those RNAs and expressed genes were categorized to five groups according to their temporal and quantitative expression patterns. Those fives groups are CPC, CPE, CPL, CPD, and CPU which represent constitutively-expressed, early-expressed, late-expressed, down-regulated, and up-regulated, respectively. Ninety two primer combinations out of a total of 192 have been tested so far. Among the twenty to fifty distinct bands per each reaction, an average of four to ten genes was identified as viral-regulated fungal genes. Those viral-specifc genes were further analyzed by DNA sequencing followed by homology search. Characterization of 30 clones including all five groups were conducted as a preliminary data and more are under investigation.

• International Journal of Industrial Entomology and Biomaterials
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• 제47권1호
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• pp.34-43
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• 2023

The eighteen (18) F₁ hybrid combinations were tested to identify potential combinations adaptable to type 1 climatic conditions in the Philippines. The six (6) bivoltine purelines (DMMMSU 108, DMMMSU 109, DMMMSU 110, DMMMSU 111, DMMMSU 113, and DMMMSU 119); and three (3) multivoltine purelines (DMMMSU 1000, DMMMSU 1007, and DMMMSU 1014), were crossed (multivoltine x bivoltine) in a mating plan. These were arranged in a Completely Randomized Design (CRD), replicated three times, and analyzed using Analysis of Variance (ANOVA). A test of significance was done using ANOVA across years and Tukey's Honest Significant Difference Test (HSD). The multiple trait evaluation index (EI) method was also used in the identification of potential F₁ hybrids. Three major phases were done: (1) parental rearing of multivoltine and bivoltine pure lines for breed multiplication; (2) hybridization process; and (3) evaluation of F₁ hybrids. Rearing evaluations were conducted for three consecutive years. Based from the three evaluations, 10 potential crosses were identified: DMMMSU MV-12, DMMMSU MV-11, DMMMSU MV-13, DMMMSU MV-16, DMMMSU MV-07, DMMMSU MV-14, DMMMSU MV-05, DMMMSU MV-09, DMMMSU MV-03, and DMMSU MV-10. The topmost combinations with the best economic and commercial characters and are consistently adaptable during two (2) cropping seasons were DMMMSU MV-07, DMMMSU MV-12, DMMMSU MV-05, DMMMSU MV-09 and DMMMSU MV-11. These newly-identified F₁ hybrids are considered potential breeds that could improve cocoon production.

• The Plant Pathology Journal
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• 제22권3호
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• pp.211-214
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• 2006

In 2004 and 2005, Phytophthora rot on Luffa cylindrica which had not been reported in Korea occurred in the experimental field at Gyeongsangnam-do Agricultural Research and Extension Services. The disease initiated on leaves and fruits of the plant with small watersoaked dark brown spots and progressed rapidly. The causal pathogen isolated from diseased tissues was identified as a Phytophthora sp. because of aseptate mycelia and zoospores released directly from sporangia. The fungus grew well on PDA and 10% V-8 juice agar showing an arachnoid or rosaceous colony pattern. Sporangia formed abundantly in water and were conspicuously papillate, noncaducous, ovoid to globose, and sized $26\sim62\times19\sim38{\mu}m$. The fungus was heterothallic as producing sexual reproduction structures only when mated with only A2 standard mating type strain. Oogonia and oospores were spherical, smooth walled, and measured as $20\sim28{\mu}m\;and\;16\sim24{\mu}m$, respectively. Oospores were aplerotic and antheridia were amphigynous, unicellula and spherical. Chlamydospores were globose and $20\sim38{\mu}m$ in diameter. Optimum temperature for growth was around $28\sim30^{\circ}C$. The fungus caused similar symptoms on artificially inoculated plant and could be re-isolated thereby proving Koch's postulation. Based on the mycological criteria investigated in this study, the causal fungus of Luffa sylindrica rot was identified as Phytophthora nicotianae. This is the first report of Phytophthora rot of Luffa cylindrica caused by P. nicotianae in Korea.