• Title/Summary/Keyword: A. radiobacter K84

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Biological control of crown gall disease on rose by Agrobacterium radiobacter K84 (Agrobacterium radiobacter K84에 의한 장미 뿌리혹병의 생물적 방제)

  • Park, Kwang-Hoon;Cha, Jae-Soon
    • The Korean Journal of Pesticide Science
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    • v.5 no.3
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    • pp.50-53
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    • 2001
  • Severe crown gall disease was occurred in green house cultivating rose in Jinchen, Chungbuk recently. Although it causes problem on rose cultivation, the growers do not have many choices of control measures for the disease now. Agrobacterium radiobacter K84 has been known as a strong antagonist against A. tumefaciens, a pathogen causing crown gall disease, and used as a biopesticide for crown gall in many countries since it had been introduced in 1972. We tested control activity of A. radiobacter K84 for the crown gall disease on rose. Spray of A. radiobacter K84 suspension on above ground of rose either before or after pathogen spray reduced size and fresh weight of galls significantly. Size and fresh weight of galls on roses inoculated with pathogen either before A. radiobacter K84 spray (pathogen-K84 treatment) or after A. radiobacter K84 spray(K84-pathogen treatment) were 4 to 5% of those of galls on roses inoculated pathogen only. Disease incidence of plants inoculated pathogen only was 85% whereas disease incidence of pathogen-K84 or K84-pathogen treatments were 6.7% and 5.0% respectively. Dipping of roots of rose in suspension of A. radiobacter K84 was also reduced size of galls and diseased rate significantly. These results indicate that A. radiobacter K84 is effective in the prevention of gall formation by A. tumefaciens and it can be used to control of crown gall disease of rose.

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Biological control of grapevine crown gall (포도나무 줄기 혹병의 생물학적 방제)

  • Chung, Kwang-Jin;Shim, Jae-Seop;Chung, Bong-Koo
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.97-101
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    • 1998
  • Agrobacterium vitis causes a crown gall disease in grapevine and that is one of the major hindrances for the wide cultivation and production of grapevine. We studied the possibility of biological control using selected biological control agent. One isolate from the infected soil, named as strain 27, was able to inhibit the biovar 1; A. tumefaciens C58 and Ach5, biovar 2; A. rhizogenes 13264, and biovar 3; A. vitis, in vitro and in vivo test. The putative biological control agent, A. radiobacter strain 27 was carrying the plasmid and the size of isolated plasmid was very similar to that of pAgK84 of A. radiobacter K84.

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