• The Korea Journal of Herbology
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• v.24 no.2
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• pp.67-76
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• 2009

Objectives : The present study compared Aralia continentalis Root and Angelica pubescens Root used in Korea and China respectively concerning their anti-oxidant effect Methods : We tested the anti-oxidant effect through in vitro experiment and in vivo experiment that induced oxidative stress using ethanol. Results : 1. DPPH scavenging activity was stronger in Aralia continentalis Root than in Angelica pubescens Root 2. Superoxide anion radical scavenging activity was similar between Aralia continentalis Root and Angelica pubescens Root 3. The linoleic acid peroxidation inhibition effect was stronger in Aralia continentalis Root than in Angelica pubescens Root. 4. The phenolic component was higher in Aralia continentalis Root than in Angelica pubescens Root. 5. Both Aralia continentalis Root and Angelica pubescens Root increased the concentration of GSH and decreased SOD activity in mice, in which oxidative stress was induced, and the effect was stronger in Aralia continentalis Root. 6. Aralia continentalis Root increased GSH peroxidase activity but Angelica pubescens Root did not have such an effect. 7. Neither Aralia continentalis Root nor Angelica pubescens Root had a significant effect on catalase, ADH and ALDH in mice, in which oxidative stress was induced. Conclusions : Aralia continentalis Root has a stronger anti-oxidant effect than Angelica pubescens Root. Thus, although Aralia continentalis Root is not an original plant recorded in botanical literature, it may be usable based on the data about its effects.

• The Journal of Korean Medicine
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• v.18 no.1
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• pp.218-237
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• 1997

In order to investigate the antihypertentive effects and its mechanism of the Siegesbeckia pubescens aqua-acupuncture treatment, experiments were performed on immediate and continuous antihypertensive effects, vasodilatation-autonomic nerve block, diuretic activity, and angiotensin converting enzyme inhibitory activity. The results were as follows; 1. Acupuncture treatment group showed significantly immediate antihypertensive effects in 4 hours after treatment. Normal saline aqua-acupuncture treatment group showed significantly immediate antihypertensive effects in 2, 4 and 6 hours after treatment. Siegesbeckia pubescens aqua-acupuncture treatment group showed significantly immediate antihypertensive effects in 30 minutes, 1, 2, 4 and 6 hours after treatment. 2. Siegesbeckia pubescens aqua-acupuncture treatment group showed significantly continuous antihypertensive effects in 6, 8, 9 and 10 days after treatment. 3. Siegesbeckia pubescens aqua-acupuncture extract solution showed significantly vasodilatatory and sympathetic nerve block effects with concentration of $10^{-6},\;10^{-5},\;10^{-4},\;10^{-3}g/m{\ell}$. 4. Oral administration group of the Siegesbeckia pubescens aqua-acupuncture extract solution didn't show no significantly diuretic effects. 5. Siegesbeckia pubescens aqua-acupuncture extract solution showed 28.8% angiotensin converting enzyme inhibitory activity.

• Journal of Korea Foresty Energy
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• v.22 no.1
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• pp.8-16
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• 2003

The Phyllostachys pubescens planted in the Nambu forest Experiment was used for this study. The growth ages of the P. pubescens were 1, 2 and 3 years, respectively. The experiment was carried out every month in between June of 2001 and May of 2002. The p. pubescens were divided into upper, middle and lower parts according to the growing points. The static modulus of elascity($MOE_d$) and dynamic modulus of elascity($MOE_d$) were investigated for the physical and mechanical properties of the P. pubescens. The density, MOEs and $MOE_d$according to the growing points were highest in the upper part of the P. pubescens. Generally, density and MOEs and $MOE_d$ of the P pubescens for 3 years grows larger every month. However, moisture content, MOEs and $MOE_d$ of the P. pubescens for 1, 2 years had nothing to do with growth ages and felling time. In the case of the relationships between average MOEs and $MOE_d$ the correlation coefficient was 0.88 in between June of 2001 and May of 2002. Also, $MOE_d$ showed about 18.5% higher than MOEs. Generally, the equally expressed in research that was known that $MOE_d$ of wood is higher than MOEs of wood. Therefore, the $MOE_d$ using a resonance frequency mode is useful as a nondestructive evaluation(NDE) method for predicting the MOE of the P. pubescens.

• Natural Product Sciences
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• v.1 no.1
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• pp.61-65
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• 1995

A new triterpenoid saponin named Mussaendoside F(1), along with five known compounds (2-6) were isolated from aerial part of Mussaenda pubescens.

• Archives of Pharmacal Research
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• v.10 no.2
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• pp.132-141
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• 1987

New triterpenoid saponins, ilexosides A, D, E, J, K and O have been isolated form the root of Ilex pubescens. Chemical and spectroscopic studies have established their structures as shown in formulae 1, 2, 8, 11, 3, 4 and 5.

• The Plant Pathology Journal
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• v.39 no.2
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• pp.191-206
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• 2023

Ground cherry (Physalis pubescens) is the most prominent species in the Solanaceae family due to its nutritional content, and prospective health advantages. It is grown all over the world, but notably in northern China. In 2019 firstly bacterial leaf spot (BLS) disease was identified on P. pubescens in China that caused by both BLS pathogens Xanthomonas euvesicatoria pv. euvesicatoria resulted in substantial monetary losses. Here, we compared whole genome sequences of X. euvesicatoria to other Xanthomonas species that caused BLS diseases for high similarities and dissimilarities in genomic sequences through average nucleotide identity (ANI) and BLAST comparison. Molecular techniques and phylogenetic trees were adopted to detect X. euvesicatoria on P. pubescens using recQ, hrpB1, and hrpB2 genes for efficient and precise identification. For rapid molecular detection of X. euvesicatoria, loop-mediated isothermal amplification, polymerase chain reaction (PCR), and real-time PCR techniques were used. Whole genome comparison results showed that the genome of X. euvesicatoria was more closely relative to X. perforans than X. vesicatoria, and X. gardneri with 98%, 84%, and 86% ANI, respectively. All infected leaves of P. pubescens found positive amplification, and negative controls did not show amplification. The findings of evolutionary history revealed that isolated strains XeC10RQ, XeH9RQ, XeA10RQ, and XeB10RQ that originated from China were closely relative and highly homologous to the X. euvesicatoria. This research provides information to researchers on genomic variation in BLS pathogens, and further molecular evolution and identification of X. euvesicatoria using the unique target recQ gene through advance molecular approaches.

• Korean Journal of Plant Resources
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• v.19 no.6
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• pp.660-664
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• 2006

The aerial part of Siegesbeckia pubescens (Compositae) has been used to treat rheumatoid arthritis and hypertension in the Oriental medicine. This crude drug has been used without process (SP-0) or with three times-process of steaming and drying (SP-3) or the nine times of that process (SP-9). To search for the antinociceptive anti-inflammatory components from this crude drug, activity-directed fractionation was performed on this crude drug. Since the $CHCl_3$ extract was shown to have a more potent effect than other extracts, it was subjected to silica gel & ODS column chromatography to yield two diterpene compounds (1). Compound 1 was structurally identified as ent-16 (H, 17-hydroxykauran-19-oic acid, which were tentatively named siegeskaurolic acid A. A main diterpene, siegeskaurolic acid A was tested for the antiiflammatory antinociceptive effects using both hot plate- and writhing anti-nociceptive assays and carrageenan-induced anti-inflammatory assays in mice and rats. Pretreatment with siegeskaurolic acid A (20 and 30mg/kg) significantly reduced the stretching episodes, action time of mice and carrageenan-induced edema. These results support that siegeskaurolic acid is a main diterpene responsible for antinociceptive and antiiflammatory action of S. pubescens. In addition, the assays on SP-0, SP-3 and SP-9 produced the experimental results that SP-9 had more significant effects than other two crude drugs. These results suggest that the processing on the original plant may lead to the higher pharmacological effect.

• Korean Journal of Plant Taxonomy
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• v.39 no.4
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• pp.299-303
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• 2009

We report an unrecorded taxon of genus Viola, V. grypoceras A. Gray var. pubescens Nakai, collected on Jeju Island, Korea. The distribution of this variety was restricted to the northern part of Japan, Hokkaido in particular. It is distinguished from V. grypoceras in that it is densely pilose except on its spur and lateral petals. We gave it the Korean name 'Tul-nak-si-je-bi-kkot' based on the morphological characters. In addition, some illustrations of this variety and a key to the V. grypoceras complex were provided.

• Microbiology and Biotechnology Letters
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• v.44 no.2
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• pp.117-123
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• 2016

In this study, to evaluate the anti-oxidative and anti-inflammatory effects of Carpinus pubescens Burkill ethanol extract (CPEE), we performed the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reactive oxygen species (ROS) inhibition, and nitric oxide (NO) scavenging assays and an analysis of the related protein expressions. CPEE showed high DPPH radical scavenging activity and effectively increased ROS inhibition activity dose-dependently. Furthermore, CPEE induced the expression of the anti-oxidative enzyme heme oxygenase 1 and its upstream transcription factor, nuclear factor-E2-related factor 2, in RAW 264.7 cells. CPEE was associated with a reduction in NO production, which was induced by lipopolysaccharide treatment in a dose-dependent manner. The expression of inducible nitric oxide synthase (iNOS), an upstream regulator of NO production, was also inhibited. Taken together, these results suggest that CPEE has anti-oxidative and anti-inflammatory activities and could be useful as a potential anti-oxidant and antiinflammatory agent.

• Korean Journal of Oriental Medicine
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• v.12 no.3 s.18
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• pp.101-115
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• 2006

The purpose of this study was to introduce the differential standard of the types of Angelicae Pubescentis Radix. We established the classificatory standard according to the external and internal morphology and the physicochemistrical pattern for the four types of Aucklandiae Radix. The results follow: 1. At the external shape, Angelica pubescens f. biserrata has rising lenticel and dark brown surface, Its section is light gray and its xylem is yellow gray. Aralia continentalis has big stem mark in upper portion, its section has no dense tissue with yellow gray. Heracleum hemsleyanum has dark brown surface and little lenticel, its cortical layer is white yellow and xylem is isabella and powdered. 2. At the internal shape, Angelica pubescens f. biserrata has phloem with half of the root and rare vessel. Aralia continentalis has phloem with two-thirds of the root and it has broad cutting and putting vessel that formed 1-2 row. Heracleum hemsleyanum also has phloem with half of the root and wide scattered latex tube and many large vessel. 3. At the TLC pattern, Heracleum hemsleyanum has remarkable dark spot at $R_{f}$ 0.23 on the sulphuric acid color pattern test, but others have faint. 4. At the HPLC pattern, all samples have generally patterns. But Angelica pubescens f. biserrata shows diminutive continentalic acid content and the peak at Rt 20.278min comes out on Heracleum hemsleyanum, but do not come out on Aralia continentalis and Angelica pubescens f. biserrata. Heracleum hemsleyanum has remarkable peak at Rt 20.278min, but shows no peak at Rt 29.023min unlike Aralia continentalis or Angelica pubescens f. biserrata. Also Aralia continentalis and Angelica pubescens f. biserrata show one remarkable peak at Rt 29.023, but Heracleum hemsleyanum do not show. Consequently, Aralia continentalis and Angelica pubescens f. biserrata are comparable whit continentalic acid content and Heracleum hemsleyanum is comparable with the peak at Rt 20.273 and Rt 29.023. So it is thought that content of continentalic acid and the peaks at Rt 20.278 and Rt 29.023 can apply to differentiate a species from other. It is considered the results of this study will be furnished the basis to succeeding studies and it is needed to extensive comparative study for the same genus-degree of relatedness.