• Korean Journal of Heritage: History & Science
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• v.47 no.2
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• pp.86-113
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• 2014

This study has researched the characteristics and elements of Chwibyeong (翠屛), a sort of trellis in the Joseon Dynasty through the old documents, and the original form of Chwibyeong at Juhapru (宙合樓) in Changdeuk Palace. The results were as follow. First, as the result of literatures analysis for Imwon-gyeongje-ji (林園經濟志) and Jeungbo-sallim-gyeongje (增補山林經濟), the plant screen was classified as kinds of support[frame] material, plants and methods of planting. It was found that the supports of Chwibyeong were made of bamboo or the material such as the Jinjangmok (眞長木: a stick of oak) and Giryu (杞柳: Salix purpurea var. japonica). The evergreen coniferous trees including Pinus densiflora, Taxus cuspidata and Thuja orientalis were mainly used for the plant material of Chwibyeong. The general planting method of Chwibyeong was to plant on the ground, but sometimes the container planting was also found on the artificial ground. Second, the term of 'Chwibyeong' in the literatures was used in only the screen made by evergreen trees, and the superordinate category term of it was indicated by 'byeong (屛)'. Therefore Chwibyeong was a compound word formed from 'chwi (翠)' which means the characteristics of evergreen and 'byeong' as tree screen which the support was made by bamboo. And Chwibyeong had semantic context which was combined with the literary symbolization to describe a landscape of green peak and Taoist ideology be inherent from 'twelve peaks of Musan[巫山十二峰]' in Sichuan sheng (四川省). Thirdly, the photograph of Chwibyeong at Juhapru taken by the 1880s, showed that Chwibyeong was made with coniferous trees and was almost 2 meters high. The Chwibyeong at Juhapru was removed during the Japanese colonial era, but a few yew trees(Taxus cuspidata) used for Chwibyeong are still remaining. And some Juniperus chinensis which the composition time is unclear, were cultivated while hung loose its branchs at the sides of Eosumun (魚水門). This Junipers were presumed to be planted by Japanese after Japanese annexation of Korea(1910), and it was judged that both of the roofs of Eosumun's side gates might have been transformed into Japanese style at the same time. Lastly, Chwibyeong at Juhapru was restored in 2008 but it was restored in wrong way from original form without precise research. Especially Chwibyeong was restored with Sasa boreralis which is damaged by frost, so it requires exertion that should revive the originals to plant original material as much as possible. And it needs the development of fabrication technique for Chwibyeong and the application to current landscape architecture.

• Korean journal of applied entomology
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• v.58 no.2
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• pp.89-99
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• 2019

In total, 654,362 adult mosquitoes were captured using black light traps in Gangwon-do Province of the Republic of Korea from 2012 to 2017. The collected mosquitoes were identified to the species level, placed in pools of up to 50 mosquitoes each, by species and date of collection, and screened for flaviviruses using a reverse transcription-polymerase chain reaction assay. A total of 276,224 adult mosquitoes were grouped in 7,721 pools for virus testing, and 68 flavivirus positive pools (0.9%) were detected. Flavivirus-positive products were confirmed by DNA sequencing. Japanese encephalitis viruses were detected in single pools collected from Chuncheon (2012, 2017: Culex pipiens, 2,728 and 1,111 mosquitoes, respectively), Hoengseong (2013: Culex orientalis, 19), and Gangneung (2017: C. pipiens, 724). All the Japanese encephalitis viruses detected were revealed as genotype V. Chaoyang viruses were detected in 63 pools of 5,055 Aedes vexans nipponii and a single pool of 585 C. pipiens collected in Gangwon-do Province from 2012 to 2017. Chuncheon was the region with the highest minimum infection rates (MIR, 0.32) and maximum likehood estimate (MLE, 0.33; confidence interval (CI) 95%, 0.23-0.46) of A. vexans nipponii for Chaoyang virus, followed by Hoengseong (MIR 0.30, MLE 0.30, CI 0.16-0.52) and Gangneung (MIR 0.21, MLE 0.21, CI 0.13-0.31). Monthly MIR and MLE values of A. vexans nipponii for Chaoyang virus were the highest in October (MIR 0.38, MLE 0.38, CI 0.07-1.25).

• Korean Journal of Environment and Ecology
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• v.34 no.6
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• pp.515-529
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• 2020

Constructed wetlands undergo biological and physical changes such as an increase in the proportion of arid plants due to the natural succession process after formation. It can adversely affect not only the purification function but also the habitat of species. As such, this study aims to identify environmental factors affecting biodiversity and propose management plans based on the monitoring results of physical environmental changes and the emergence of species in seven constructed wetlands selected based on the water depth and surrounding conditions among the lands purchased by the Nakdong River basin. We examined the environmental conditions and emergence of the Odonata, which is a wetland-dependent species, to predict the trend of changes in biodiversity and abundance. The results showed that the open water area decreased as the emergent plants spread to the deep water in 2015 compared to 2012 when they were initially restored to a depth of 0.2 to 1 m. While a total of 54 dragonfly species were observed, the habitat diversity, such as vegetation, water surface, and grassland, remained similar to the initial formation of the wetlands despite the expansion of the emergent plants. On the other hand, the number of Agrionidae species, which prefer areas with fewer aquatic plants, decreased between 2012 and 2015 due to the diminished water surface. The p-values of the differences in the number of species and population between wetlands by year were 2.568e-09 and 1.162e-08, respectively, indicating the statistically significant differences. The decrease in open water surface was found to have the greatest effect on the biodiversity and habitat density of dragonflies. The time-series survey of constructed wetlands confirmed that the spread of Phragmites communis, P. japonica, Typha orientalis, etc., caused a decrease in species diversity. It suggests that environmental management to maintain the open water surface area is necessary.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.116-186
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• 1983

An epidemiological study on Clonorchis sinensis infection along the Nam-river (total length ; 186km) flowing in Gyeongsang-nam-do, southern part of Korea, was carried out. Formalin-ether concentration technique and Stoll's egg counting method were employed to figure out the prevalence of C. sinensis infection. For the detection of cercariae from Parafossarulus sp., the snail host of C. sinensis, each snail was placed in aerated tap water, and examined for expelled cercariae. For observing the metacercariae the fresh water fish favorably eaten in raw conditions wore prepared by means of pressing the muscles between two slide glasses and/or by digesting them with artificial gastric juice. The fresh water fish were fed to the rabbits to get the worms and to identify the morphology of adult C. sinensis. The results are summarized as follows: 1. Overall Prevalence of C. sinensis infection was 38.75 from 5,291 examinees; 44.15 (1,408 out of 3,196) in male and 30.4% (637 out of 2,095) in female. 2. The prevalence rates were 42.0% at the upper stream, 41.2% around the vicinity of Jinyanglake, 34.2% at Jinju city, 34.2% at middle stream and 40.3% at down stream regions, respectively. 3. By age, the highest positive rate (53.4∼54.3%) was observed in 30 to 59 years of age. In this age group, the rate in males was 59.7∼62.2%, and in females 42.2∼44.4%, In the age group of less than 19 years it was 7.5∼20.9%. 4. By social strata, the positive rate was 16.5% in the primary school children, 22.6% in school students, 46.2% in teachers and local officers and 49.6% in the general inhabitants. 5. The quantitative examinations with the stool collected from clonorchiasis cases revealed that the light infection (less than 4,000/EPG) was 53.6%, moderate infection (4,001∼10,000 /EPG) 30.3% and heavy infection (more than 10,001/EPG) 16.1%, respectively. More than half of total cases examined were light infection, and 73.2% of female examinees were lightly infected with this cuke. 6. The average value of EPG was 4,963 (male, 6,057; female, 2,557 and the highest value was obtained from the age group of 30 to 59 years (5,240∼6,454). 7. The prevalence of Metagonimus yokogawai infection in humans was 5.5%, and 89.8% of metagonimiasis cases wore double-infected arith C. sinensis. The highest prevalence rate was observed in Jinju city (11.2%). 8. Total of 5,005 Parafossarulus sp., the snail intermediate host of C. sinensis, were examined for the detection of cercariae. The cercarial expulsion rate was 0.34%, and the snails collected in Jin-yang-lake side and in the down stream expelled mainly the cercariae of C, sinensis. 9. About 788 cercariaejday (range: 127∼1,503) were expelled daily from a snail naturally infected with C. sinensis. The snails which released more than 1,000 cercariae/day were 30.8% out of total collected. A snail uniquely released 5,840 cercariae/day in this study. 10. The other trematode cercariae besides C. sinensis were also detected, and the rates out of total snails were the cercariae of Losogenes liberum 6.71%, Cyathocotyle orientalis 0.26%, Notocotylus attenuatus 2.52% and Mucobucaris 0.54%, 11. Ten out of 18 species of fresh water fish caught along the river harbored the metacercariae of C, sinensis. The highest rate of metacercarial infection in fish was detected in Pseudorasbora larva (85.9%). The fish mainly eaten by the inhabitants along the Nam-river, and the metacercarial infection rates were: Zacco platypus 8.0%, Hemibarbus sp. 18.2∼26.7%, Gnathepcgcn sp. 37.5%, Ischikauia steenackeri 42.9% and Pseudogobio esocinus 16.7%. 12. Out of 36 P. larva, the number of metacercariae were about 109 (ranEe; 18∼446) per fish and 27 per gram of flesh. The fish caught in spring harbored the highest number of metacercariae. As indicated above, the prevalence of C. sinensis infection in the inhabitants residing around the Nam-river was relatively high. The farther toward the upper stream areas, the higher was the positive rate of C. sinensis infection, and most of clonorchiasis cases were lightly infected. The snail hosts of C. sinensis distributed all around the Nam-river. Several species of freshwater fish were infected with the metacercariae of C. sinensis and the infection rates were relatively high. To prevent C. sinensis infection in the endemic areas, the effective health education system is suggested as a control measure, although mass treatment is also expected to be useful, using chemotherapeutic agents such as "praziquantel", a recently developed anthelminthic for C. sinensis inftraction.

• Applied Microscopy
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• v.15 no.2
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• pp.31-68
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• 1985

Authors are trying to unveil the ultrastructural organization of olfactory bulb, which has been summerized under light microscopic level or communicated only in some detail in different view point until now. For the critical point of view, since the phylogenetical approach will give the ultimate value in the correlative study between structural and functional bases (Brodal, 1969), the present study was carried out light and electron microscopic analyses of the structures of the neurons and synaptic organizations in olfactory bulbs from different animals in phylogenetical scale. We selected each one species from five animal classes: the house rabbit(Oryctolagus cuniculus var. domesticus [Gmelin]) from Mammalia, the domestic fowl (Gallus gallus domesticus Brisson) from Aves, the viper (Agkistrodon hylys [G.P. Pallas]) from Reptilia, a frog (Bombiana orientalis Boulenger) from Amphibia and the crussian carp (Carassius carassius [Linne]) from Pisces. For light microscopic study, samples were fixed in 10% formalin and paraffin sections were stained with hematoxylin-eosin. For the electron microscopic study, the tissues were fixed by perfusion through the heart or immersion with 1% paraform-aldehyde-glutaraldehyde mixture (phosphate buffer, pH 7.4), and final tissue block trimmed under dissecting microscope were osmicated (1% OsO4), they were embedded in Araldite or Epon 812, and ultrathin sections were made by LKB-V ultratome following the inspection of semi-thin sections stained with toluidine blue-borax solution. Ultra-thin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100CX electron microscope. We have summerized our morphological analyses as follows: 1. The olfactory bulb of rabbit, viper and frog shows the eight layers of fila olfactoria, glomerular, external granular, external plexiform, mitral cell, internal plexiform, internal granular, medullary but domestic fowl shows the five layers of glomerular, fibrillar, mitral, granular and medullary and the three layers of fibrilla, glomerular and medullary in crussian carp. The sharpness of demarcation between the layers shows deferential tendency according to phylogenetical order. 2. Mitral cells of vertebrate have large triangular or oval shape with spherical nuclei which contain not so much chromatin. The cytoplasm contains numerous cell organelles, of which Nissl's bodies or granular endoplasmic reticula arranged as parallel strands. Development of granular endoplasmic reticula were declined as the phylogentical grade is going lower. 3. Tufted cells of all animal are mostly spindle or polygonal contour and contain oval nuclei which located in periphery of cytoplasm. The nuclei of rabbit, fowl, viper and frog has relatively space chromatin, but a nucleus of crussian carp contain irregularly aggregated chromatin in karyoplasm. Their cytoplasmic volume and cell organelle contents are in between those of mitral cell and granular cell. They contain moderate amount of mitochondria, granular endoplasmic reticula, a few Golgi complex, polysomes, lysosome, etc. 4. Granule of cells of all the vertebrate amimals studied exhibit similar features; cells and their dense nuclei show spherical or oval contour, and they have the thin rim of cytoplasm which contain only a few cell organelles. 5. In rabbit, the soma of mitral cells were in contact with boutons with two types of synaptic vesicles, that is, round and flat vesicles, especially flat vesicles in boutons were showing reciprocal synapses. However, in domestic fowls, vipers, frogs and crussian carps, there were found boutons showing only spherical synaptic vesicles. 6. The boutons containing round synaptic vesicles were made contact with the some of tufted cell of olfactory bulb in the rabbits, fowls, vipers and frogs, but no synaptic boutons were observed in soma of tufted cells in crussian carps. In the frogs, there were observed dendrites were contact with the soma of tufted cells. 7. In the neuropils of plexiform, granular and glomerular layers olfactory bulbs in the vertebrate, the synapses were axo-large dendrites, axo-median and small dendrites, dendrodendritic, and axo-axonal contacts. However, in the neuropil of crussian carps, synapses were observed only in glomerular layer.

• Korean Journal of Environment and Ecology
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• v.34 no.1
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• pp.85-92
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• 2020

The purpose of this study is to provide base information that can be utilized in surveying the status of landscape management within apartment complexes and grasping the conditions of the soil for planting plants, as a way to improve the quality of green spaces by repairing poorly vegetated spaces within apartment complexes and remodeling them with diverse species of plants. Considering that trees and shrubs of equal sizes are simultaneously planted upon the completion of apartment complexes, they were divided based on their completion year at the interval of 5 years and differences in the growth of trees and the components of soil over time were analyzed. Out of the species of trees planted in all the 9 apartment complexes surveyed in this study, the growth of 4 species of needleleaf trees and 4 species of broadleaf trees were surveyed. Juniperus chinensis 'Kaizuka' and Pinus densiflora Siebold & Zucc. out of the needleleaf trees and Malus floribunda Siebold ex Van Houtte out of the broadleaf trees showed the highest growth rate when over 5 years passed after planting and their growth rate decreased when over 10 years passed. Platycladus orientalis and Acer palmatum Thunb. in the apartment complexes that were built over 10 years ago showed the highest growth rate, which indicates that the species require a relatively long period of time for growth. The hardness of the soil at the areas where trees were planted but their soil surface was bare was analyzed. When over 5 years passed after the completion of apartment complexes, over 20 mm of the soil was found to be stamped. The physicochemical properties of soil were also surveyed and the pH level was found to have been continuously high ever since the completion. The organic content in the surveyed soil was about 1/3 of the content in fertile soil, which means that additional fertilization is required. These results indicate that the stamped soil and the health of soil can be restored, when replanting plants in bare areas, by adding plans to improve soil, such as designing drain ways around the planted areas, transporting soil for the 50 cm depth of the ground and mixing organic matters such as chaff, and simply by planting groundcover plants in the lower part of tress and shrubs.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.4
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• pp.277-287
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• 2012

To develop new therapeutic materials to prevent hair loss and enhance hair growth, we developed a medicinal herbal complex extract (MHCE) using 23 herbs traditionally used in oriental medicine. Medicinal Herbal complex extract was consist of Angelica gigas Nakai, Psoralea corylifolia Linne, Biota orientalis Endlicher, and Eclipta prostrata Linne, Rehmannia glutinosa Liboschitz var. purpurea Makino, Ligustrum lucidum Aiton, Polygonum multiflorum Thunberg, and Sesamum indicum Linne, Sophora angustifolia Sieboldet Zuccarini, Angelica dahurica Benthamet Hooker, and Leonurus sibiricus Linne, Salvia miltiorrhiza Bunge, Prunus persica Batsch, Commiphora molmol Engler, Chrysanthemum indicum Linne, Boswellia carterii Birdwood, Panax ginseng C. A. Meyer, Cnidium officinale Makino, Albizia julibrissin Durazzini, and Corydalis ternata Nakai that have traditionally been used for treating hair loss, preventing gray hair, anti-inflammation, and blood circulation in oriental medicine. In addition, we examined the hair growth effect of MHCE in vitro and in vivo. In vitro, we evaluated the effects of MHCE on cultured HFDPC, HaCaT cells, and murine embryonal fibroblasts (NIH3T3 cells). Also, we evaluated the ability of MHCE to prevent gray hair on murine melanoma cells (B16F1 cells). The hair growth-promoting effect of MHCE in vitro was also observed in vivo using C57BL/6 mice. Our results showed that MHCE significantly increased the proliferation of HFDPC (175 % proliferation at $50{\mu}g/mL$), HaCaT cells (133 % proliferation at $20{\mu}g/mL$), and NIH3T3 cells (120 % proliferation at $50{\mu}g/mL$). MHCE also showed consistent melanogenesis in B16F1 cells (154 % melanin synthesis at $50{\mu}g/mL$). Moreover, MHCE showed potential for hair growth stimulation in C57BL/6 mice experiments (98 % hair growth area on 4 weeks). These results indicate that MHCE may be a good candidate for promotion of hair growth.

• The Korean Journal of Zoology
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• v.27 no.2
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• pp.117-125
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• 1984

Mosquitoes were collected with light trap at a pig shed in the vicinity of Daegu city from mid-April to the end of November in 1981 and 1982. A total of 12,942 and 118,061 individuals were collected in 1981 and 1982 respectively. The collection comprised 77% females and 23% males in 1981, and 96% females and 4% males in 1982. The catches were classified into following 7 species: Culex (Culex) pipiens pallens, C. (C.) tritaeniorhynchus summorosus, Anopheles (Anopheles) sinensis, C. (C.) vagans, C. (C.) oritntelis, C. (C.) bitaeniorhynchus, Aedes (Aedimorphus) vexans nipponii. The former three species showed distinct seasonal prevalence. Arranged in the descending order in size of the catches, in 1981, Culex (Culex) pipiens pallens was 44.9% of the total collection (at sex ratio of 0.85), Anopheles (Anopheles) sinensis 42.9% (0.05), Culex (Culex) tritaeniorhynchus summorosus 12.1% (0.00). On the contrary, Culex (Culex) tritaeniorhynchus summorosus 70.4% (0.00), Anopheles (Anopheles) sinensis 25.2% (0.05), Culex (Culex) pipiens pallens 4.4% (2.19) in 1982. The monthly percentages of collected mosquitoes to the total collection were 0.1% (in 1981) and 0.0% (in 1982) in May; 3.5%, 1.3% in June; 50.0%, 33.9% in July; 37.1%, in August; 8.8%, 11.9% in September; 0.5%, 0.8% in October and 0.0% in November. As for seasonal prevalence, mosquitoes appeared in May in both years and began to increase in number from the first week of June in 1981, but from the second week of May, ahead of three weeks in 1982. The highest peak time in 1982 was the second week of August, two weeks later than the fifth week of July in 1981. Culex (Culex) pipiens pallens showed the maximum activity for the fifth week of July in 1981, but for the third week of July, ahead of two weeks in 1982. Culex (Culex) tritaeniorhynchus reached the highest peak for the second week of August in both years. Anopheles (Anopheles) sinensis showed the maximum activity for the fifth week of July in 1981, but for the third week of July, two weeks earlier in 1982. The highest peak times of three main species were compared respectively as folows. Culex (Culex) pipiens pallens had the highest peak time in common with Anopheles (Anopheles) sinensis, Culex (Culex) tritaeniorhynchus summorosus showed the maximum activity for the second week of August in 1982.

• Journal of Life Science
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• v.20 no.5
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• pp.729-735
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• 2010

In our previous study, it was reported that an herbal mixture, SH21B, inhibits fat accumulation and adipogenesis both in vitro and in vivo models of obesity. SH21B is a mixture composed of seven herbs: Scutellaria baicalensis Georgi, Prunus armeniaca Maxim, Ephedra sinica Stapf, Acorus gramineus Soland, Typha orientalis Presl, Polygala tenuifolia Willd, and Nelumbo nucifera Gaertner (Ratio 3:3:3:3:3:2:2). The aim of this study was to investigate the detailed molecular mechanisms of the effects of SH21B on various regulators of the adipogenesis pathway. During the adipogenesis of 3T3-L1 cells, SH21B significantly decreased the expression levels of central transcription factors of adipogenesis, such as peroxisome proliferator-activated receptor (PPAR)$\gamma$ and CCAAT/enhancer binding protein (C/EBP)$\alpha$. To elucidate the detailed molecular mechanism of the anti-adipogenic effects of SH21B, we examined the expression levels of the various pro-adipogenic or anti-adipogenic regulators of adipogenesis upstream of $PPAR{\gamma}$ and C/$EBP{\alpha}$. The mRNA levels of Krox20 and Kruppel-like factor (KLF) 15, which are pro-adipogenic regulators, were significantly down-regulated by SH21B treatment, whereas the mRNA levels of C/$EBP{\gamma}$ and KLF5 were not changed. KLF2 and C/EBP homologous protein (CHOP), which are anti-adipogenic regulators, were significantly up-regulated by SH21B treatment. These results suggest that the molecular mechanism of the anti-adipogenic effect of SH21B involves both the down-regulations of pro-adipogenic regulators, such as Krox20 and KLF15, and the up-regulations of anti-adipogenic regulators, such as KLF2 and CHOP, which results in the suppression of central transcription factors of adipogenesis including $PPAR{\gamma}$ and C/$EBP{\alpha}$.

• Korean Journal of Ecology and Environment
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• v.54 no.1
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• pp.24-38
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• 2021

Microhabitat In the upper stream is created by various environment variables such as the bottom substrate and the physicochemical factors, and may influence the distribution of benthic macroinvertebrates. We investigated the bottom substrate and environmental variables influencing the distribution of benthic macroinvertebrate in 26 stream-type waterways established at upper reaches of Geum River. During study period, total 85 families, 160 species, 9305 individuals of benthic macroinvertebrates were recorded. The stream-type waterways, where the bottom substrates consist mainly of pebble (16~64 mm) and cobble (64~256 mm) or with rapid water velocity (more than 0.2 m/s) and high dissolved oxygen (more than 120%), were supported by high species diversity of benthic macroinvertebrate. Hierological cluster analysis and the nonparametric multidimensional scale (NMDS) divided 26 stream-type waterways into a total of three clusters. In Cluster 1, the invertebrate species, such as Branchiura sowerbyi, Cloeon dipterum, Ischnura asiatica, Paracercion calamorum, and Radix auricularia, closely related to aquatic macrophytes, and Chironomidae spp., Limnodrilus gotoi, and Tanypodinae sp. were abundant in waterways, with high coverage of silt and clay as well as high turbidity and total nitrogen. The benthic macroinvertebrate species (Cheumatopsyche brevilineata, Drunella ishiyamana, Dugesia japonica, Ephemera orientalis, Gumaga KUa, Macrostemum radiatum, Potamanthus formosus, Semisulcospira libertine, Stenelmis vulgaris, and Teloganopsis punctisetae) included in Cluster 2 were dominated in sites with high cover rates of pebble and gravel. Cluster 3 was predominantly covered by the Cobbles, was supported by Simulium sp. Such a clear distinction in the study sites means that each stream-type waterways is governed by a clear habitat environment. In the case of some sites with low species diversity, improvement measures are required to restore nature, such as improving the function of inflows and outflows, creating meandering channel, and inducing the settlement of littoral vegetation.