• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.565-569
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• 2008

Geographic variation of characteristics of cone, seed and bract morphology were examined in 8 populations of rare endemic Abies nephrolepis (Trautv.) Maxim and A. koreana Wilson. Additionally we studied classification index to distinguish between the species by the method of discriminant analysis. Nested ANOVA showed that there were statistically significant differences among populations as well as among individuals within populations in all 13 morphological traits. In the seed length, seed index, bract width, and bract index of A. nephrolepis and the bract width and index of A. koreana, variance components among populations were larger than those among individuals within populations. In discriminant analysis, three traits (cone width, length of seed wing, and bract length) were found to be useful in discriminating A. nephrolepis from A. koreana. The optimal classification results of stepwise selection were discriminated length of seed wing and bract length.

• Korean Journal of Environment and Ecology
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• v.5 no.1
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• pp.32-41
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• 1991

A survey of subalpine zone (altitude is 1,360-1,410m) in Mt. Chiri, was conducted using 25 sample plots of 500$m^2$ size. The classification by TWINSPAN and DCA ordination were applied to the study area in order to classify them into several groups based on woody plants and environmental variables. By TWINSPAN techniques, the plant community were divided into five groups by importance value of Abies nephrolepis. The dividing groups are Fraxinus rhynchophylla - Magnolia sieboldii community, Quercus mongolica - Rhododendron schlippenbachii community, Q. mongolica - F. rhynchophylla - Acer pseudo-sieboldianum community, Q. mongolica- A. nephrolepis- Rh. schlippenbachii community, and A. nephrolepis- Pinus densiflora - Rh. schlippenbachii community. The successional trends of tree species by both techniques seem to be from Q. mongolica to A. nephrolepis in the canopy layer. It was no difference between the stand scores of DCA and environmental variables.

• Korean Journal of Plant Taxonomy
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• v.35 no.4
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• pp.287-294
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• 2005

Three unrecorded fern species from the flora of Korea, Nephrolepis cordifolia, Athyrium epirhachis, and Asplenium castaneo-viride, are reported here. Nephrolepis cordifolia, recognized as introduced species and cultivation in Korea until now, is found in the wild at the entrance of small cave in Jeju island. Athyrium epirhachis is found under the Quercus acuta forest at about 400 - 500 m alt. in Jeju island. This species is similar to A. otophorum but different from the latter by having once pinnately divided leaf blade and the decurrent base of ultimate segment. Asplenium castaneo-viride is hybrid between A. ruprechtii and A. incisum and is distinguished from parental species by presence of proliferation, shape of leaf blade, pattern of venation, and leaf texture.

• Journal of Life Science
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• v.34 no.3
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• pp.160-169
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• 2024

In this study, Abies nephrolepis MAX. was divided into A. nephrolepis MAX. stem (AS) extract and A. nephrolepis MAX. leaf (AL) extract. Their anti-inflammatory abilities and applicability as cosmetic materials were determined. Tests of the cell survival rate measured using RAW 264.7 cells and extracts of AS and AL showed 97.8% and 95.6% cell viability at a 500 ㎍/ml concentration. To determine anti-inflammatory activity, we examined the inhibitory effects on the production of LPS-induced NO in RAW 264.7 cells by Griess assay. The results showed that the AS and AL extracts presented a concentration-dependent inhibition of NO production. The protein expression inhibitory effects of AS and AL extracts were measured by western blot at 25, 50, and 100 ㎍/ml concentrations. β-actin was used as a positive control. The results of western blot of extracts from AS showed that the expression inhibition rate of the iNOS protein was decreased by 50.1% at the 100 ㎍/ml concentration. Additionally, the results of western blot of AL extracts showed that the expression inhibition rate of COX-2 and iNOS protein was decreased by 66% and 8.2% at the 100 ㎍/ml concentration. The mRNA inhibitory effect was measured by RT-PCR at 25, 50, and 100 ㎍/ml concentrations. GAPDH was used as a positive control. Consequently, the iNOS mRNA expression effect by RT-PCR of AS extract demonstrated by RT-PCR decreased by 27.9% at the 100 ㎍/ml concentration, and the iNOS and IL-6 mRNA expression effect of AL extract measured by RT-PCR decreased by 48.6% and 48.7% at the 100 ㎍/ml concentration.

• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.577-584
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• 2011

To estimate level of genetic variation and genetic differentiation among populations of 3 populations in Abies koreana and 5 populations in Abies nephrolepis, 5 nSSR markers were analyzed. Except 1 locus where too many alleles were observed excessively, population genetic parameters were recalculated with 4 loci. Mean expected heterozygosities ($H_e$) were 0.292 in A. koreana and 0.220 in A. nephrolepis, respectively. In both species, positive fixation coefficient was estimated (F=0.065 for A. koreana and F=0.095 for A. nephrolepis), which suggests that there is an excess of homozygotes relative to Hardy-Weinberg expectations within populations. Relatively high degree of population differentiation was observed in A. koreana ($F_{ST}=0.063$). compared to that of A. nephrolepis ($F_{ST}=0.039$). From 3-level Hierarchical estimation of F-staticstics, only 4.9% of the genetic variation was allocated between species ($F_{PT}$), which suggested that most of genetic variation was shared between two species. On the basis of results from analysis of genetic relationships among populations, 2 populations of A. koreana (Mt. Halla and Mt. Deogyu) were genetically distinct from the populations of A. nephrolepis but a population of Mt. Jiri was allocated within a group of populations of A. nephrolepis. Populations of both species seemed to have undergone genetic drift due to gradual decrease in population size induced by global warming after the last glacier, which resulted in increase of homozygotes by inbreeding. It could be also postulated that these species might be diverged recently and It is likely that the two species have not fully speciated yet.

• Journal of Korean Society of Forest Science
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• v.81 no.4
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• pp.310-319
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• 1992

Vegetational data obtained from 24 quadrats of Mt. Paekdu area were analysed by applying two multivariate methods : two-way indicator species analysis(TWIMSPAN) for classification and detrended canonical correspondence analysis(DCCA) for ordination. DCCA technique allowed to extract the ordination axes that could be related to 9 environmental factors. The dominant tree species distributed in the region between 1,700-1,950m from sea level were Betula ermanii, Larix olgensis, Abies nephrolepis, and Picea jezoensis in descending order. The dominant tree species distributed in the region between 1.300-1.500m were Abies nephrolepis, Pinus koraiensis, Larix olgensis, Betula mandshurica, and Picea jezoensis in descending order. The available phosphorus content in forest soil ranged from 7 to 1.153ppm. The distribution of the major communities showed a close relationship with elevation gradient. From high to low elevation, the communities of Alnus fruticosa var. mandshurica, Betula ermanii, Larix olgensis, Abies nephrolepis, Picea jezoensis, Pinus koraiensis, and Quercus mongolica were found in descending order. At the communities where Betula ermanii was dominant, Betula ermanii forest was distributed in the high elevation area, Betula ermanii-Larix olgensis forest in the good nutrition of organic matter and $K^+$, and Abies nephrolepis forest in the good nutrition of $Mg^{{+}{+}}$ and $Ca^{{+}{+}}$. The most dominant factor influencing community distribution was elevation.

• Journal of Korean Society of Forest Science
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• v.96 no.4
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• pp.387-392
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• 2007

Characteristics of needle morphology and anatomy were examined in 14 populations of Abies nephrolepis (Trautv.) Maxim. and A. koreana Wilson. Additionally we studied the classification index to distinguish between the species by the method of discriminant analysis. Characteristics of needle for A. nephrolepis could be distinguished from those for A. koreana by flatten arrangement, thin and long length for needle form, many stomata row, and marginal position of resin duct Nested ANOVA showed that there were statistically significant differences among populations as well as among individuals within populations in all 9 needle traits. For the needle indices such as needle thickness, number of stomata row, and the distance between resin duct and vascular for both species, variance components among populations were larger than those among individuals within populations. The characteristics that contributed most to the separation of A. nephrolepis and A. koreana according to the discriminant analysis using stepdisc procedures were needle index and thickness of needle, needle arrangement index, distance between resin duct and vascular, and number of stomata row.

• Journal of Korean Society of Forest Science
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• v.112 no.4
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• pp.405-416
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• 2023

In this study, the sustainability of Abies nephrolepis in the presence of Tripterygium regelii was analyzed. To accomplish this, a field survey of140 stands was conducted. Based on the presence or absence of T. regelii, as well as the layer in which it was present (where appropriate), the type of T. regelii presence wasclassified as follows: TP type, indicating the presence of T. regelii in the tree and sub-tree layers; SP type, indicating its presence in the shrub layer; and A type, indicatingits absence from all layers. The indicator species analysis revealed T. regelii to be an indicator species of the TP type and SP type. The MRPP test was used to examine the differences among the types. Moreover, the seedling and DBH class analysis indicated there to be a difficulty in terms of the status competition, as the number of young trees generated by A. nephrolepis was the lowest in the TP type. The findings of this study enhanced our understanding of the vegetation structure of A. nephrolepis, thereby contributing to efforts concerningits conservation and management.

• Research in Plant Disease
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• v.12 no.2
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• pp.115-118
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• 2006

In February of 2000-2001, the gray mold disease occurred on nephrolepis (Nephrolepis sp.) grown in a flower nursery farm in Suwen, Korea. Typical symptoms were water-soaked brown or blackish lesions on terminal leaf blades. Severely infected leaves were entirely blighted with grayish fungal mycelia formed on the surface. Conidia of the fungus in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and $13.5{\sim}16.9{\times}6.8{\sim}9.2{\mu}m$ in size. Conidiophores were formed on PDA with $8.7{\sim}11.1{\mu}m$ in width. The sclerotia were readily formed within 2 or 3 days on PDA. In addition, the Biolog database gave the causal fungus a high similarity to Botrytis cinerea (78%) with a match probability of 100%. Pathogenicity of the causal organism was proved according to Koch's postulate. The causal organism was identified as Eotrytis cinerea based on its mycological characteristics and utilization of carbon sources with Biolog system as supporting data. This is the first report of gray mold of nephrolepis caused by Botrytis cinerea in Korea.

• Journal of Life Science
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• v.32 no.6
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• pp.430-437
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• 2022

We conducted a study on the whitening activity of Abies nephrolepis MAX. extracts. The electron-donating ability of 70% ethanol extracts from A. nephrolepis MAX. Stem (AN-S) and A. nephrolepis MAX. Leaf (AN-L) were found to be 89.4% and 90.9% at 1,000 ㎍/ml concentration, respectively. Their ABTS⁺ radical scavenging abilities were found to be 88.8% and 96% 500 ㎍/ml concentration, respectively. Their tyrosinase inhibitory effects were found to be 48.5% and 31.1% at 500 ㎍/ml concentration, respectively. Cell survival rates measured in B16F10 were examined with the AN-S and AN-L extracts. Results showed cell viabilities of 98.3% and 94.4% at 500 ㎍/ml concentration, respectively. The protein and mRNA expression inhibitory effects of AN-S and AN-L extracts were measured by Western blotting and RT-PCR, respectively, at 25, 50 and 100 ㎍/ml concentrations. The results of western blotting showed that the AN-S extract at 100 ㎍/ml concentration decreased the TRP-1 protein expression rate by 46%. The results of Western blotting also showed that the AN-L extract at 100 ㎍/ml concentration decreased the MITF protein expression rate was by 54.6%. Consequently, in the case of MITF, TRP-1, TRP-2, and tyrosinase, the amount of mRNA expressed decreased as the concentration of the extracts increased. Our results suggested that A. nephrolepis MAX. extracts can be used as a functional cosmetic material by confirming that they can be used as a whitening material.