• Journal of the Korean Wood Science and Technology
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• v.41 no.6
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• pp.551-558
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• 2013

Ethanol extracts from Rhododendrom brachycarpum and Abies koreana were investigated for their anti-allergic activities using RBL-2H3 cell line. After treatment with ethanol extracts of various concentrations on the immune response induced mast cell by concanavalin A (Con A), the expressions of cytokine interleukin-4 (IL-4), interleukin-13 (IL-13) were determined by using RT-PCR and the degranulation of mast cells was determined by measuring ${\beta}$-hexosaminidase release. Expression level of IL-4 was decreased by the extract from Rhododendrom brachycarpum in $10^{-7}$, $10^{-5}$ and $10^{-3}%$ concentrations. Expression level of IL-13 was also decreased by both extracts. ${\beta}$-Hexosaminidase release by RBL-2H3 cells was inhibited at the $10^{-5}$ and $10^{-3}%$ concentration of extracts from Rhododendrom brachycarpum and Abies koreana, respectively. These results demonstrate that ethanol extracts of Rhododendron brachycarpum and Abies koreana exert anti-allergic effects by regulating the reduction of IL-4 and IL-13 genes expression and also the secretion of ${\beta}$-hexosaminidase.

• Journal of Life Science
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• v.32 no.1
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• pp.56-62
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• 2022

This study was investigated to evaluate the potential of Stewartia koreana Nakai as an oral healthcare material. The inhibitory effects of extracts on the biofilm formation and fimbriae genes expression of Porphyromonas gingivalis were determined by minimal inhibitory concentrations (MIC), biofilm biomass staining, SEM, and qRT-PCR analysis. The S. koreana Nakai branch was extracted into 70% ethanol, and bacteriostatic MIC of extracts against P. gingivalis were 0.6 mg/ml. In P. gingivalis cultures treated with 0.2-2.0 mg/ml of extract, biofilm production rate was significantly decreased in a concentration-dependent manner. The morphology of treated and untreated samples was observed by SEM, and cell aggregation and biofilm were only observed in those treated with extract. Subsequently, qRT-PCR analysis showed that the mRNA expression on fimbriae genes fimA and fimB was suppressed in a concentration-dependent manner. Based on these results, it can be suggested that S. koreana branch extract has the potential to be used as naturally derived oral healthcare material because of its bacteriostatic action and inhibition of P. gingivalis biofilm formation.

• The Korean Journal of Ecology
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• v.14 no.4
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• pp.379-398
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• 1991

In order to investigate the ecotypic variations of pulsatilla koreana nakai distributed in korea, 8 local populations of the plant were selected in accordance with latitude and compared on the differences of leaf shape, elongstion, stomatal distribution and dhlorophyll content. The incised angle of the plant is 38。32' in cheju, showing a great difference as compared with 23。77'-30。 32' of the other sites. as to the incised rate, mt. togyu and cheju representsed relatively high values of 49.04% and 48.21% the parted leaved individuals were only observed along the warm current coastal area including cheju, sokcho and sunchon and were 54(62.8%), 2(2.5%) and 3(3.3%) in the sites, respectively. Especially, the individuals consisting of only parted learves were 5(5.8%) in cheju alone and none of the other sites showed such individuals at all. So p. cernua distributed in cheju and p. koreana in inland area can be considered to beecotypic variations of the same species from the upper investigations. According to the elongations of leaf and rachis, the local populations could be classified into 3 types, such as warm current coastal area type(cheju,sunchon and sokcho),southern inland area type(andong, chungju and mt. togyu)and central inland ara type(mt. komdan and hongchon). The dentate numbera of 39.80-43.73 in the warm current area were also different from those of 87% in mt.togyu and 15% in hongchon, while opposite leaflets account for 100% in the other sites. The content of chlorophyll ranged from 0.609 mg/g. d.w.(cheju)to 0.924 mg/g.d.w.(hongchon), showing an increasing trends as the latitudes grow higher. The number of stomata on the low epidermis is much larger than that on the upper epidermis in the whole area. But both aspects showed an increasing tendency, too as the latitude becomes higher.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.537-541
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• 1994

This study was conducted to investigate the possibility of callus induction and plant regeneration from immature inflorescence, stem and petiole of A. koreana MAX. which is worth enough to be used as food and medicine. The callus induction and its proliferation was best when immature inflorescence segments were placed on MS medium supplemented with 2, 4-D 2mg / l. The white and compact embryogenic callus on the surface of dark yellow and soft callus which was induced from immature inflorescence segments came into being only on MS medium with 2, 4-D 1mg /l and 2mg /l, but didn't come into being on the other ones. The shoot came into being effectively from callus derived from immature inflorescence on MS medium mixed 2, 4-D 0. 1mg /l with Kinetin 1mg /l, and 2, 4-D 0.5mg /1 with Kinetin 2mg /l. Immature inflorescence was most appropriate material for callus induction and plant regeneration.

• Applied Biological Chemistry
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• v.42 no.4
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• pp.344-350
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• 1999

The mutagenicity, antimutagenicity, and cytotoxicity of the hot water extracts of 130 medicinal plants were examined. The results obtained using authentic alkaline phosphatase activity as the growth representative of the indicator cell, E. coli PQ37, demonstrated that the extracts of 14 medicinal plants including, Forsythia koreana Nakai, showed strong toxic effect on the cell growth, however, the extracts of 22 medicinal plants, including Phlomis umbrosa Turcz., were shown to have stimulatory effect on the cell growth. The mutagenicity and antimutagenicity of the medicinal plants were screened using SOS chromotest. The mutagenic activity was detected from 36 kinds of the samples, including Forsythia koreana Nakai and Coptis japonica Nakai. On the contrary, 12 medicinal plants, including Crataegus pinnatifida Bunge, were shown to have a significant antimutagenic activity.

• Journal of Korean Library and Information Science Society
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• v.35 no.2
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• pp.73-94
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• 2004

This study is made of proofreading some documents about ‘Tripitaka Koreana’, which was unknown and not noticed quite well until now and is also for montemplating the features of ‘Qidanzang’ that were accepted in ‘Tripitaka’. By all the researches the following facts were found out. First, ‘Qidanzang’ included in ‘Tripitaka’ were all 18 cases. Second, ‘Qidanzang’ that were corrected and accepted into ‘Tripitaka’ were 42cases. Third, ‘Qidanzang’ that have errors at comparing documents were all 11cases. Fourth the sutra that were accepted in the comparative book were all 57 cases. Fifth, unaccepted sutras , which were found out at comparative documents, were 4cases. At last, from the analysis abouve, we notice that most of the ‘Qidanzang’, not part of it, were accepted into ‘Tripitaka Koreana’ and like the previous viewpoint, ‘Qidanzang’ was an excellent book but it also had a few mistakes.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.2
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• pp.41-51
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• 1996

Phaseolus aureus (mung bean), Leguminosae, has been used as an antidote from the ancient time. Especially, it has been widely used for cleaning face and skin in oriental countries. Although several constituents such as fatty acids, phytoallexin and phaseol derivatives were reported in P. aureus and related species including seedlings, there has been a few report to describe its biological activity. Therefore, in this investigation, the ethanol extract from P. aureus was obtained and its biological activities including the antioxidative and anti-inflammatory activities were studied. The 70% ethanol extract from P. aureus showed dose-dependent antioxidative activity (52.3% inhibition at 4 mg/ml) against lipid peroxidation assay, while the extract did not show the inhibitory activity of superoxide radical formation. The extract also showed the topical anti-inflammatory activity against croton-oil and arachidonic acid induced mouse ear edema test (18-19% inhibition at 7.5 mg/ear) as well as mild inhibitory activity against picryl chloride induced delayed hypersensitivity in mouse. For investigating active principles, vitexin and isovitexin (apigenin C-glycoside) as flavonoids, and adenosine were isolated from the extract using silica gel chromatography. The actual contents of vitexin and isovitexin were found to be 3.7 and 2.4 mg/g extract, respectively. Vitexin and isovitexin showed the antioxidative activity. They showed the topical anti-inflammatory activity, although the activities were not potent compared to the reference compounds. These results suggested that vitexin and isovitexin may be, at least in part, the compounds contributing the antioxidative activity in vitro and the topical anti-inflammatory activity of P. aureus in vivo. All results of present study might be one of the scientific rationale in using mung bean for skin care from the ancient time.

• Korean journal of applied entomology
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• v.57 no.4
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• pp.303-315
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• 2018

Bonsai is part of export forest products in Korea. Demand for bonsai exports to other countries is growing but the need for quarantine hazards must be eliminated in other to export. Plant parasitic nematode is also an important factor in quarantine. The nematodes were surveyed from bonsai farms in 6 areas of Korea. The surveyed deciduous bonsai plants were Acer palmatum, Zinnia elegans, Ligustrum obtusiflium, Carpinus turcaninowii, and Malus sieboldii. Three plant parasitic nematodes (Hemicyliophora koreana, Rotylenchus blothrotylus and Xiphinema americanum) and three non-parasitic nematodes (Aporclaimellus donghwaens, Egtitus andhricus, and Mesodorylaimus usitatus) were isolated from bonsai. Non-parasitic nematode, Mesodorylaimus usitatus was isolated from all surveyed bonsai trees. Only two species of quarantine nematodes (Hemcylicopora koreana and Xiphinema americanum) were found from bonsai. A. donghwaens and M. usitatus were isolated from root of bonsai tree. However other nematodes were isolated from soil in bonsai trees.

• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.304-308
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• 2021

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

• Journal of Food Hygiene and Safety
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• v.27 no.1
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• pp.96-102
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• 2012

This study was designed to evaluate a repeated oral dose toxicity and immunomodulating activity of $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ in Sprague-Dawley rats. The female rats were treated with $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ of control group, low group (0.5 ml/kg), medium group (1 ml/kg), high group (2 ml/kg) for distilled water, intragastrically for 4 weeks, respectively. To ensure the safety of $Pulsatilla$ $koreana $ and $Artemisiae$ $annuae$ such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. Hematological results were the increase of neutrophils, lymphocytes and monocytes in the high dose group of $Pulsatilla$ $koreana$. The increase immune cells in the high dose group of $Pulsatilla$ $koreana$ might immunomodulating activity. No significant differences in body weight, feed intake, serum biochemical analysis and histopathological between control and fed group were found. In conclusion, $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ is physiologically safe and improve immunomodulating activity.