• Bulletin of the Korean Chemical Society
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• v.31 no.5
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• pp.1159-1164
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• 2010

Several triterpenoid saponins from root of Pulsatilla koreana Nakai (Ranunculaceae) were studied and their biological activities were reported. It is difficult to analyze triterpenoid saponins using HPLC-UV due to the lack of chromophores. So, evaporative light scattering detection (ELSD) is used as a valuable alternative to UV detection. More recently, a charged aerosol detection (CAD) has been developed to improve the sensitivity and reproducibility of ELSD. In this study, we developed and validated a novel method of high performance liquid chromatography coupled with a charged aerosol detector for the simultaneous determination of four triterpenoid saponins: pulsatilloside E, pulsatilla saponin H, anemoside B4 and cussosaponin C. Analytes were separated by the Supelco Ascentis$^{(R)}$ Express C18 column (4.6 mm ${\times}$ 150 mm, 2.7 ${\mu}m$) with gradient elution of methanol and water at a flow rate of 0.8 mL/min at $30^{\circ}C$. We examined various factors that could affect the sensitivity of the detectors, including various concentrations of additives, the pH of the mobile phase, and the CAD range. Linear calibration curves were obtained within the concentration ranges of 2 - 200 ${\mu}g$/mL for pulsatilloside E, anemoside $B_4$ and cussosaponin C, and 5 - 500 ${\mu}g$/mL for pulsatilla saponin H with correlation coefficient ($R^2$) greater than 0.995. The limit of detection (LOD) and quantification (LOQ) were 0.04 - 0.2 and 2 - 5 ${\mu}g$/mL, respectively. The validity of the developed HPLC-CAD method was confirmed by satisfactory values of linearity, intra- and inter-day accuracy and precision. This method could be successfully applied to quality evaluation, quality control and monitoring of Pulsatilla koreana.

• Archives of Pharmacal Research
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• v.27 no.9
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• pp.915-918
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• 2004

By bioassay-guided separation, an already known saponin, Pulsatilla saponin D was isolated from the root of Pulsatilla koreana Nakai as a antitumor component when evaluated by in vivo antitumor activity as well as in vitro cytotoxic activity test. It showed potent inhibition rate of tumor growth (IR, 82％) at the dose of 6.4 mg/kg on the BDF1 mice bearing LLC cells.

• Animal Systematics, Evolution and Diversity
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• v.28 no.4
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• pp.297-303
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• 2012

New Korean lumbricids are described: Eisenia gaga Blakemore sp. nov. from remote Gageodo Island and E. sindo Blakemore, sp. nov. from an island at Incheon. Both are comparable to Eisenia koreana (Zicsi, 1972) from near Pyongyang, North Korea. A remarkable yet previously unrecognized similarity to American earthworm Eisenoides carolinensis (Michaelsen, 1910) is discussed, but synonymy is rejected on tenuous morphological grounds (form of nephridial vesicle bladders) with taxonomy supported by objective molecular data (mtDNA cytochrome c oxidase subunit 1 barcodes).

• The Korean Journal of Zoology
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• v.35 no.1
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• pp.107-114
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• 1992

A new species, Psylla Koreana, arising on the Acer is described from Korea, and nineteen psyllid species from Mt. Sobaek are listed.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.169-173
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• 2020

We report on an unrecorded endophytic fungus, Grovesiella abieticola (Zeller & Goodd.) M. Morelet & Gremmen isolated from Abies koreana of Mt. Halla in Jeju. In this study, we used a culture method to determine its conidia and compared relative species using an internal transcribed spacer (ITS) 1, 2, and 5.8S ribosomal DNA sequence. Thus, we present the cultural characteristics and morphology of G. abieticola in this paper.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.6
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• pp.135-149
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• 2015

Korean fir(Abies koreana E.H.Wilson 1920), endemic tree species of Korean peninsula, is considered as vulnerable and endangered species to recent rapid environmental changes such as land use and climate change. There are limited activities and efforts to find natural habitats of Korean fir for conservation of the species and habitats. In this study, by applying SDMs (Species Distribution Models) based on climate and topographic factors of Korean fir, we developed Korean fir's predicted distribution model and explored novel natural habitats. In Mt. Shinbulsan, Youngnam region and Mt. Songnisan, we could find korean fir's two novel habitat and the former was the warmest($13^{\circ}C$ in annual mean temperature), the driest(1,200mm~1,600mm in annual rainfall) and relatively low altitude environment among Korean fir's habitats in Korea. The result of SDMs did not include mountain areas of Gangwon-do as habitats of A. nephrolepis, because there were different contributions of key habitat environment factors, summer rainfall, winter mean temperature and winter rainfall, between A. koreana and A. nephrolepis. Our results raise modification of other distribution models on Korean fir. Novel habitat of Korean fir in Mt. Shinbulsan revealed similar habitat affinity of the species, ridgy and rocky site, with other habitats in Korea. Our results also suggest potential areas for creation of Korea fir's alternative habitats through species reintroduction in landscape and ecosystem level.

• Journal of Veterinary Clinics
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• v.12 no.1
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• pp.911-916
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• 1995

Ionophorous antibiotics have been used popularly for the protection of avian coccidiosis, though Halo-fuginone which is derived from an extract of the Dichroa febrifuga, was developed as an antimalarial and anticoccidial agent. The antibiotics are regarded as the causes of residues in the avian products, therefore the author has tried to find out more safe herbal materials for the control of avian coccidiosis. Thus, the extracts of 5 kinds of herbs, roots of Pulsatilla koreana Nakai and Sophora flavescens Aiton, nuts of Quisqualis indica Linne, whole herbs of Artemisia annua Linne and Polygonum aviculare Linne, were investigated on the efficacy against E tenella. Survival rates, bloody diarrhea, lesion scores, body-weight gains and feed conversion rate were investigated at the 1st and the 2nd week after infection. The bloody diarrhea in the groups treated with P koreana, A annua, Q indica and P aviculare were milder than those in the groups treated with S flavescens and infected control. The lesion score in the groups treat.4 with herb extracts(2.00$\pm$0.82~2.65$\pm$0.89) were lower than those in the control group (2.80$\pm$0.7l). But, there was not significant in all infected groups. The body weight gai in the groups treated with P koreana, S flavescens, A annua and Q indica(291.74$\pm$15.76~303.43$\pm$51.90) were higher than that in the control group (283.71$\pm$16.53) but there was not significant. In a conclusion, analyzing the data of the survival rates, bloody diarrheal symptoms, lesion scores, body weight gains and oocyst excretions, the extract of S flavescens, P koreana, A annua and Q indica were effective. The further research on the above herbal materials will have been carried out by the author and the other researchers by means of the chemical analysis of the components.

• Korean Journal of Plant Resources
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• v.20 no.1
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• pp.1-6
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• 2007

The vegetation of habitats of a Korean rare plant Kirengeshoma koreana Nakai at Mt. Baekun was investigated by phytosociological approach. The vegetation of investigated area shows typical southern-temperate Quercus forest. The humidity of soil was high and the ground of locality was flat area of sub-alpine. These investigated area is very important for conservation of a rare plant, Kirengeshoma koreana that is limited in this area. Therefore, sustainable conservation strategy such as designation of conservation area should be prepared urgently by the results of long-term monitoring studies.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.1
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• pp.58-65
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• 1999

The purpose of this study was to evaluate the effect of rehabilitation measures on the fired woodland of Chesuk-bong in Mt. Chiri through the vegetation structure and comparison of growth pattern between natural seedlings and transplanted Abies koreana seedlings. It was also discussed the rate of standing and fallen trees of dead conifers in the management strategy for vegetation regeneration and protection of sub-alpine area. In this fired woodland, shrub layers consisting of Weigela subsessilis, Sambucus williamsii var. coreana, Rhododendron schlippenbachii, R. mucronulatum, Tripterygium regelii, and etc. were gradually expanded, and tree species such as Betula ermani, Sorbus commixta, Acer pseudosieboldianum, and Malus baccata var. mandshurica were also regenerated. The growth of height and crown width of Abies koreana seedlings transplanted for vegetation regeneration were a little lower than those of natural seedlings. The seedlings of A. koreana transplanted in this fired woodland grew about 50.6% normally, but the others had multi-branched or growth stopped by death of terminal shoot. Because the number of dead conifers by fire tend to be gradually increased as time passed, it can occurs to the soil erosion and landslide by weakness of the cohesion and resistance of soil. Therefore, it is consider that rehabilitation measures projects of the fired woodland in subalpine area may be more prevention a natural disaster like soil erosion, flood and landslide.

• Journal of the Korean Wood Science and Technology
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• v.45 no.5
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• pp.629-639
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• 2017

Tripitaka Koreana in Haein Temple, Hapcheon Province is the most historical and largest heritage in this country, however the species of their blocks have yet been unknown. A nondestructive test method is necessary to investigate their species. The oven-dry density of wood was measured by inversely using the principle of high frequency moisture meter. The oven-dry densities of more than 100 domestic species of specimens estimated by measurement method and high frequency moisture meter were compared and following conclusions were obtained. There was highly close correlation between the oven-dry density estimated by measurement method and the oven-dry density estimated by high frequency moisture meter. The densities of Tripitaka Koreana that was a global cultural heritage could be correctly estimated by using high frequency moisture meter and the equilibrium moisture content under which Tripitaka Koreana equilibrated, thus, it was expected to provide the key to species identification.