• YAKHAK HOEJI
• /
• v.49 no.1
• /
• pp.6-10
• /
• 2005

Immunoglobulin Y (IgY) obtained from chicken as the immunization host brings several advantages to antibody production, such as improved yield, lower cost, longer stability and higher specificity than mammalian immunoglobulin. In the present study, we attempted to produce IgY against a sialic acid-binding lectin, Maackia fauriei agglutinin (MFA), from egg yolk of white Leghorn hens. For the isolation of IgY from egg yolk, we applied a water dilution method. The weekly yield of IgY was determined by enzyme-linked immunosorbent assay, with a final yield of anti-MFA IgY from total IgY of approximately $1\%$. The yielded IgY were used to prepare IgY-affinity column conjugated with CNBr-activated Sepharose 4B, which resulted in the lectin being successfully purified in a single step from Maackia fauriei. This purified lectin exhibited the same hemagglutination activity as lectin purified using conventional purification methods.

• Korean Journal of Medicinal Crop Science
• /
• v.24 no.6
• /
• pp.464-470
• /
• 2016

Background: Valeriana fauriei (Valerianaceae) has been used to as a traditional medicine to treat a variety of symptoms, including headache, insomnia, hypertension, and menstrual irregularity. However, the present study investigates the species' antioxidant activity and its inhibition of oxidative DNA damage, which have yet to be studied. Methods and Results: The antioxidant activity was assessed using radical scavenging assays with 1,1-diphenyl-2-picryl hydrazyl (DPPH) and, 2, 2'-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and a reducing power assay. The total phenol content was also analyzed, and phenolic compounds were detected using HPLC/UV, whereas the inhibitory effect of Valeriana fauriei on oxidative DNA damage was measured using ${\phi}-174$ RF I plasmid DNA cleavage assay. The DPPH and ABTS radical scavenging activity were $75.17{\pm}3.55%$ and $95.83{\pm}0.63%$, repectively, and the reducing power was $93.14{\pm}1.74$ at $200{\mu}g/m{\ell}$. The total phenol content was $10.24{\pm}0.04mg/g$, whereas chlorogenic acid, catechin, caffeic acid and epicatechin were identified using HPLC/UV, and the ${\phi}-174$ RF I plasmid DNA cleavage assay indicated that V. fauriei provided protection against oxidative damage. Conclusions: The results of the present study suggest that V. fauriei has powerful antioxidant activity that can provide protective effects against the oxidative DNA damage caused by free radicals. The species, therefore, provides a valuable resource for the development of natural pharmaceutical to treat aging, cancer, and degenerative diseases.

• Korean Journal of Plant Taxonomy
• /
• v.42 no.4
• /
• pp.330-334
• /
• 2012

Distribution of Juncus fauriei L$\acute{e}$v. & Vaniot were restricted in Japan and Russia in previous literatures. We found its new distribution in the Korean peninsula: wet meadows of sandy seashore in Ha-Jo-Dae, Kangwon province. The plants have flattened stems (usually coiled) and black-colored margin in tepals. These are key characters to distinguish J. fauriei from J. decipiens, J. setchuensis, J. haenkei, and J. brachyspathus, which are closely related Korean taxa in the section Juncotypus. We provide description, distribution, and detailed photos of J. fauriei, and key for Korean taxa of sect. Juncotypus. The plant is named as "Geom-Jeong-Nap-Jak-Gol-Pul" in Korean.

• Korean Journal of Plant Taxonomy
• /
• v.44 no.4
• /
• pp.257-260
• /
• 2014

While preparing a floristic study of Korean Pteridophytes, we discovered the following two unrecorded species from Jeju Island: Cyclosorus penangianus (Hook.) Copel. and Pteris fauriei Hieron. C. penangianus can be distinguished from other Korean congeners of the genus Cyclosorus by having exindusiate sori, toward with sinus of ultimate segments of veinlets more than 4. P. fauriei can be distinguished from other Korean congeners of the genus Pteris by having ultimate segments with entire margins, veins reaching to leaf margin and membraneous petiolar scales.

• Korean Journal of Medicinal Crop Science
• /
• v.3 no.2
• /
• pp.77-80
• /
• 1995

This study was carried out to know the effect of air temperature on photosynthesis and growth of Korean Valerian (valeriana fauriei var. dasycarpa HARA). The results are as follows; 1. Photosynthesis of V fauriei had highly significant relation to temperature in a quadratic regression mo­del, from which the optimum temperature for the plant growth were estimated to be $17.7^{\circ}C$. During the day­time in the field condition, the photosynthesis rate was highest at 9 a.m, then reduced to minimum at 2 p.m., and increased again thereafter. 2. Number of stomata was about $25/mm^2$ on the upper side and $85/mm^2$ on the lower side of the leaf, and the size was $21{\sim}30/{\mu}m$ in diameter. 3. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was $20.3^{\circ}C$.

• Korean Journal of Medicinal Crop Science
• /
• v.4 no.1
• /
• pp.7-11
• /
• 1996

This study was conducted to know the effects of light intensity and temperature on growth of V. fauriei plants. Photosynthesis of V. fauriei had highly significant relations to light intensity and temperature in a quadratic regression model, from which the optimum light intensity and temperature for the plant growth were estimated to be 40,000lux and $17.7^{\circ}C.$ Root was produced less by shading at Jinbu where is located in alpine region, but root yield is increased by shading at Umsong where is located in plane region. Roots were produced more in Jinbu than in Umsong. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was $20.3^{\circ}C.$

• Korean Journal of Medicinal Crop Science
• /
• v.4 no.2
• /
• pp.109-113
• /
• 1996

This study was conducted to know the effect of cultivation area with different sea levels on essential oil content and its composition and content of major active compounds in Valerian fauriei var. dasycarpa HARA and Valerian officinalis L. The total contents of essential oil were not different according to the locality in both valerian, however, its compositions were varied. The major components in V. fauriei were detected bornyl acetate and sesquiterpene alcohol in three cultivation areas. The major compounds were borneol, borney acetate, ${\beta}\;-sesquiohellandrene$ in V. officinalis other. Among the valepotrate components known as major pharaceutically active components in Europe and Nepal valerian, valtrate was detected in a small amount in V. fauriei, but its quantity was not changed according to the cultivation area.

• 한국약용작물학회:학술대회논문집
• /
• 2011.04a
• /
• pp.188-189
• /
• 2011

• Korean Journal of Pharmacognosy
• /
• v.26 no.2
• /
• pp.175-178
• /
• 1995

The study was conducted to investigate the effect of cultivation area with different sea levels on essential oil in the root of Valeriana fauriei var. dasycarpa Hara (Valerianaceae). The total content of the essential oil was not different in various cultivation area, however, its composition varied. The major components were bornyl acetate and sesquiterpene alcohol in three cultivation areas. Among the valepotrate components known as major active components in Europe and Nepal valerian, valtrate was detected in a small amount, but the quantity was not changed.

• 한국약용작물학회:학술대회논문집
• /
• 2011.09a
• /
• pp.198-199
• /
• 2011