• 한방비만학회지
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• 제22권2호
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• pp.115-124
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• 2022

Objectives: In this study, we investigated physicochemical characteristics and antioxidant activity of Artemisia argyi H. fermented with lactic acid bacteria. Methods: The A. argyi water extract was fermented using lactic acid bacteria isolated from kimchi at 30℃ for 96 h. To evaluate the physicochemical characteristics, we investigated pH, total acidity, viable cells, free sugars, free organic acids, and free amino acids contents during fermentation. In addition, we examined antioxidant activity of fermented Artemisia argyi H. by measurement of 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazinyl (DPPH) and 2,2'-azubi-bus-3-ethylbenzothiazoline-6-sulfonic acid (ABTS⁺) scavenging activities. Results: During fermentation time, pH of fermented A. argyi was decreased from 4.57 to 3.22, and total acidity was increased from 0.39% to 1.63%. The number of lactic acid bacteria fermented A. argyi was increased from 1.28×10⁷ CFU/ml to 3.75×10⁸ CFU/ml during fermentation time. The free sugars of fermented A. argyi were confirmed glucose and sucrose. In addition, the organic acid content of fermented A. argyi was the highest in oxalic acid and lactic acid. In the composition of free amino acids, content of ornithine increased from 4.4 mg/100 g to 18.8 mg/100 g compared with non-fermented A. argyi. Furthermore, DPPH and ABTS⁺ radical scavenging activities of fermented A. argyi increased in a dose-dependent manner. Conclusions: In conclusion, our data suggest that lactic acid fermentation of A. argyi could be used as a functional food for antioxidants.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.492-502
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• 2023

This study investigated the antioxidant and immune enhancement activities of Artemisia argyi H. fermented by Lactobacillus plantarum. The fermented A. argyi H. ethanol extract increased scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺), hydroxyl (·OH), and superoxide (O₂^-) radicals. Particularly, the ethanol extract of fermented A. argyi H. exhibited higher ·OH and O₂^- radical scavenging activities, compared with DPPH and ABTS⁺ radical scavenging activities. To evaluate the immune enhancement effects of the fermented A. argyi H., mice were fed a normal diet supplemented the fermented A. argyi H. at concentrations of 1%, 2%, and 5%, respectively. The supplementation of fermented A. argyi H. dose-dependently increased splenocyte proliferation. In addition, mice fed with 5% fermented A. argyi H. showed enhanced proliferation of T-cells and B-cells, along with increased levels of interferon-γ, interleukin-10, and tumor necrosis factor-α, compared to the normal group. Furthermore, mice fed with fermented A. argyi H. exhibited an increase in prominent probiotics such as Akkermansia muciniphila and Lactobacillus in gut microbiota, compared to the normal group. This study suggests that fermented A. argyi H. with Lactobacillus plantarum could be used as a dietary antioxidant and immune enhancement agent.

• Plant Resources
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• 제5권2호
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• pp.161-164
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• 2002

This study was performed to investigate the effect of Artemisiae argyi Herba extract on the treatment of chromaticity and colorfastness. Artemisiae argyi Herba has been used as a Korean medicine. It is effective in removing skin disorders and suppressing pain. It is also good as a fungicide and in treating pruritis. In the long history of Korea, dyeing has been applied for a means representing the grace of natural and inner esthetic consciousness of man. Vegetable dyes give us such great benefits, diversified color; but no pollution. And ramie fabric has distinctive features such as beautiful brilliance, elegance, and strong durability. So, it is regarded as a special product of Korea traditionally. These studies were carried out to treat with acetate iron, dichloride copper and alum with a mordant to ramie fabric. The ramie fabric was died with Artemisiae argyi Herba extract. The results of experiment showed as follows: First, the chromophoric degree was the highest in acetate iron but not distinction in another mordants. Seocond, the light colorfastness was the highest in non treated and dichloride copper, but alum was the lowest. Third, the discoloration was alum and dichloride copper showed first grade in washing colorfastness. Abrasion colorfastness was not significant in this test. According the previous results, Artemisiae argyi Herba has an efficiency in removing skin disorders and suppressing pain. So it is considered that Artemisiae argyi Herba can be applied effectively to theraphy of fungicide and in treating pruritis.

• 인간식물환경학회지
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• 제23권5호
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• pp.537-544
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• 2020

Background and objective: Artemisia argyi has a long history as an effective treatment for various diseases. The detection of environmental pollutant benzo(a)pyrene, a known human carcinogen, in the leaves of Artemisia argyi is cause for concern. For medicinal plant extracts, both a reduction of benzo(a)pyrene as well as the maintained effectiveness of the compound are important. Therefore, in this study, we propose an optimized process for the addition and filtration of activated carbon to reduce benzo(a)pyrene and change the contents of the indicating substance(jaceosidine and eupatilin). Methods: Artemisia argyi EtOH extract containing 36 ppb of benzo(a)pyrene was added to 0.1, 0.5, 1.0, and 1.5% (w/w) of activated carbon for 120 min and filtered using an activated carbon filter 1, 2, 3, and 5 times respectively. The content of benzo(a)pyrene and indicating substances in Artemisia argyi extract were then measured with high performance liquid chromatography (fluorescence and UV detectors). Results: As the amounts of activated carbon powder and filtering cycles increased, the content of benzo(a)pyrene in the Artemisia argyi extract decreased. However, when activated carbon powder 1.5% was added to the extract, and when the activated carbon filter was filtered five times, the results were reduced by 15% and 30~40% respectively. The optimal extraction condition for reducing benzo(a)pyrene was adding 1.5% of activated carbon powder. This resulted in reducing benzo(a)pyrene by 83% and indicating substances by about 4%. Conclusions: Here we present a process for reducing benzo(a)pyrene in Artemisia argyi extract using activated carbon to reduce toxicity and minimize the loss of active ingredients. This approach has potential application within a manufacturing process of various medicinal plant extracts.

• 한국식품영양학회지
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• 제17권1호
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• pp.86-91
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• 2004

강화에 자생하는 사자발쑥(Artemisia princeps), 황해쑥(Artemisia argyi) 그리고 인진쑥(Artemisia iwayomogi)의 에탄을 추출물과 열수 추출물을 각각 제조하여 항산화 효과와 아미노산 및 당 성분을 분석하였다. 총 페놀함량은 사자발쑥 10.2 mg/$m\ell$, 황해쑥 4.7 mg/$m\ell$ 그리고 인진쑥 7.0 mg/ml로 나타나 사자발쑥의 총 페놀함량이 가장 많았다. 플라보노이드함량은 사자발쑥 6.1 mg%, 황해쑥 3.6 mg% 그리고 인진쑥 1.8 mg%로 나타나 역시 사자발쑥의 함량이 가장 많았다. DPPH법에 의한 전자공여능 측정 실험에서 사자발쑥 19.0%, 황해쑥 24.4% 그리고 인진쑥 14.3%로 나타나 황해쑥의 항산화 능력이 다소 높았다. HPLC를 이용한 아미노산분석에서 총 아미노산함량은 사자발쑥이 가장 많았으며 모든 쑥에서 proline이 비교적 많았다. 당분석에서는 총 당함량은 인진쑥이 가장 많았고 모든 쑥에서 당성분중 glucose가 가장 많았으며 fructose는 인진쑥＞황해쑥＞사자발쑥 순이었다.

• 생명과학회지
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• 제24권4호
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• pp.377-385
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• 2014

본 연구는 남해특화작목인 섬애약쑥의 활용도를 높이고 건강식품 소재로서의 가능성을 제시하기 위하여 음건한 섬애약쑥과 일정기간 발효시킨 섬애약쑥의 이화학적 성분을 비교하고, 물과 에탄올 추출물(30%, 50%, 70%, 100%)을 제조하여 항산화 활성과 tyrosinase 및 xanthine oxidase 저해 활성을 측정하였다. 수율은 섬애약쑥 30% 에탄올 추출물에서 29.74%로 가장 높았으나 총 페놀 및 플라보노이드 함량은 섬애약쑥 70% 에탄올 추출물에서 각각 72.25 mg/g 및 33.34 mg/g으로 가장 높았다. 항산화 활성을 측정한 결과 모든 추출물에서 농도가 증가함에 따라 그 활성이 증가하였으며, 특히 섬애약쑥 70% 에탄올 추출물은 DPPH, ABTS 라디칼 소거능 및 ${\beta}$-carotene linoleic 탈색 방지 효과가 가장 우수하였다. Tyrosinase 저해 활성은 $500{\mu}g/ml$ 농도에서 섬애약쑥 70% 에탄올 추출물이 50.01%로 가장 높았으나 100% 에탄올 추출물은 11.44%로 가장 낮았다. Xanthine oxidase 저해 활성은 $250{\mu}g/ml$ 농도에서 섬애약쑥 물 추출물이 60% 이상으로 가장 높았다. 이상의 결과를 종합하여 볼 때, 섬애약쑥은 발효하지 않았을 때 항산화 활성이 더 우수하였으며, 70% 에탄올로 추출할 경우 가장 항산화 효과가 높았다. 또한, 섬애약쑥 추출물은 tyrosinase 및 xanthine oxidase 저해에도 상당한 효과를 가지며, 이러한 결과는 페놀 및 플라보노이드 함량과 상관관계가 높은 것으로 판단된다.

• Korean Journal of Acupuncture
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• 제21권1호
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• pp.15-27
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• 2004

Objectives : Acupuncture has been used to prevent and treat the cerebrovascular accident, such as a stroke, and many studies of acupuncture and moxibustion concerning to the stroke have been undertaken in the human and various animals. Also, herbal acupuncture, namely aqua acupuncture has been applied and developed to various diseases including the cerebrovascular accident. FOLIUM ARTEMISIAE ARGYI is the dry leaf of Artemisia argyi Levl. et Vant. collected in summer before the plant blooms and used to moxibustion and has been recommended for use as an analgesic and hemostatic. In this study, effects of FOLIUM ARTEMISIAE ARGYI (艾葉)' herbal acupuncture on the $LR_3$, namely Taechung on neuroprotection after the transient forebrain ischemia were investigated in Sprague-Dawely rats. Methods : Expressions of neuronal nitric oxide synthase (nNOS) protein in the hippocampus and cortex were observed at 2 hrs after transient forebrain ischemia by immunohistochemistry. Results : Expression of nNOS protein was increased in the hippocampus and cortex at 2 hrs after transient forebrain ischemia. However, pretreatment with FOLIUM ARTEMISIAE ARGYI' herbal acupuncture on $LR_3$ significantly decreased expression of nNOS protein protein compared to ischemia group. These features were observed in the motor cortex and the hippocampus. Conclusions : These results suggest that pretreatment with FOLIUM ARTEMISIAE ARGYI' herbal acupuncture on $LR_3$ inhibits the expression of nNOS protein induced by transient forebrain ischemia and may modulate excitatory toxicity of neuron related to neuronal cell death.

• 한국식품영양학회지
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• 제18권4호
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• pp.334-340
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• 2005

[ $CCl_4$ ]에 의한 실험동물의 간 손상에 미치는 사자발쑥 (Artemisia princeps)과 황해쑥 (A. argyi)의 영향을 알아보고자 쑥 분말을 투여한 실험동물 혈청의 간 기능 지표 효소인 aspartate aminotransferase (AST), alanine aminotransferase (ALT), 그리고 alkaline phosphatase (ALP) 활성을 측정하였다. $CCl_4$ 단독 투여군에 비해 $CCl_4$와 사자발쑥 혼합병행군은 AST, ALT 및 ALP 활성을 각각 33, 23 및 $19\%$ 감소시켰고 $CCl_4$와 황해쑥 혼합 병행군 역시 각각 37, 33 및 $26\%$ 감소시켰다. 사자발쑥과 황해쑥을 에탄올로 추출하여 제조한 쑥 추출물에 대한 항산화 성분을 측정한 결과, 총 페놀 함량은 사자발쑥 10.2 mg/mL, 황해쑥 4.7 mg/mL로 나타나 사자발쑥의 함량이 보다 많았으며 플라보노이드 함량은 사자발쑥 $6.1\;mg\%$, 황해쑥 $3.6\;mg\%$로 나타나 역시 사자발쑥의 함량이 보다 많았다. DPPH법에 의한 전자 공여능 측정 실험에서 사자발쑥 $19.0\%$, 황해쑥 $24.4\%$로 나타나 황해쑥의 항산화 능력이 다소 높았다. 사자발쑥과 황해쑥의 추출된 휘발성 성분의 조성을 GC/ MS로 분석한 결과, 사자발쑥의 주요 향기 성분은 테르펜류의 $\delta$-3-carene ($2.2\%$)과 알코올류의 nerolidol ($0.9\%$)로, 황해쑥은 알코올류의 eugenol ($1.4\%$)과 에스테르류의 ethyl pentadecanoate ($1.1\%$)로 확인되었다.

• BMB Reports
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• 제32권6호
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• pp.585-593
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• 1999

The water and methanol extracts of Artemisia argyi showed significant cytotoxicities against J774A.1 cells but not so much against normal leukocytes. The cytotoxicities were found to be dependent on the extract concentration and the incubation time. The concentration of water and methanol extracts inhibiting 50% of cell proliferation ($IC_{50}$) were estimated to be 44.2 mg/ml and 71.6 mg/ml, respectively. In the presence of Artemisia argyi water extract, total superoxide dismutase (CuZnSOD and MnSOD) activities of media, cytoplasmic and mitochondrial fractions of J774A.1 cells increased in accordance with cytotoxicity. MnSOD was found to be the main component of enhanced total SOD activities, particulary in the mitochondrial fraction. In contrast to SOD, catalase and glutathione peroxidase (GPx) were not found in any instance of the current investigation. In addition, substantial amount of $O_2^-$ appeared to be generated in the mitochondrial fraction under the influence of Artemisia argyi. All data put together, it is postulated that Artemisia argyi extracts seem to stimulate $O_2^-$ generation in mitochondria of J774A.1 cells with concomitant increases of SODs. Since $H_2O_2$, the reaction product of SOD on $O_2^-$, is known to be readily converted to very toxic $OH{\cdot}$ in the absence of catalase and/or GPx cooperation, toxicity derived from ROS such as $O_2^-$, $H_2O_2$, and $OH{\cdot}$ may be the main cause of necrosis and/or apoptosis of J774A.1 cells.

• 대한한의학회지
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• 제24권2호
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• pp.81-93
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• 2003

Objectives : Recently, the new therapeutic tool, that is herbal acupuncture, has been developed and applied to various diseases including the cerebrovascular accident. The main characteristics of herbal acupuncture are a combination of acupuncture and herbal medicine. It was not well known the therapeutic effect and the mechanism of herbal acupuncture on transient forebrain ischemic injury, although it has been used frequently in clinics. The objective was to determine the effect of folium artemisiae argyi (艾葉) herbal acupuncture on the trasient forebrain ischemic injured rat. Methods : In this study, the effects of folium artemisiae argyi (艾葉) herbal acupuncture on the $LR_3$ named Taechung, on neuroprotection after the transient forebrain ischemia were investigated in Sprague-Dawely rats. Expressions of cFos, FosB and BDNF protein in the hippocampus and cortex were observed at 2 hrs and 48 hrs after transient forebrain ischemia by immunohistochemistry and ELISA technique. Results : Expression of cFos protein was increased slightly in the hippocampus and cortex at 2 hrs after transient forebrain ischemia, but FosB protein was increased highly comparing to cFos protein. However, pretreatment with folium artemisiae argyi' herbal acupuncture on $LR_3$ significantly increased expression of cFos protein and significantly decreased expression of FosB protein compared to control group. These features were observed in the retrosplenial granular cortex as well as the hippocampus. Also, pretreatment with folium artemisiae argyi' herbal acupuncture on $LR_3$ significantly increased the expression of BDNF protein in the hippocampus ($263.26{\pm}44.56{\;}pg/ml$ extracted by water, $275.35{\pm}51.47{\;}pg/ml$ extracted by moxa tar)and the cortex ($102.33{\pm}13.65{\;}pg/ml$ extracted by water, $109.54{\pm}9.37{\;}pg/ml$ extracted by moxa tar) compared to the hippocampus ($134.07{\pm}2.96{\;}pg/ml$) and the cortex ($61.16{\pm}4.11{\;}pg/ml$) in control group at 48 hrs after transient forebrain ischemia. Conclusions : These results suggest that pretreatment with folium artemisiae argyi' herbal acupuncture on $LR_3$ has neuroprotective effect on transient forebrain ischemia and the herbal acupunture on $LR_3$ may be related to antioxidative function of folium artemisiae argyi.