• Journal of Microbiology and Biotechnology
• /
• 제19권9호
• /
• pp.972-981
• /
• 2009

In this paper, a fed-batch cultivation process in recombinant Escherichia coli BL21(DE3) bacteria, for the production of human soluble catechol-O-methyltransferase (hSCOMT), is presented. For the first time, a straightforward model is applied in a recombinant hSCOMT expression system and distinguishes an initial cell growth phase from a protein production phase upon induction. Specifically, the kinetic model predicts biomass, substrate, and product concentrations in the culture over time and was identified from a series of fed-batch experiments designed by testing several feed profiles. The main advantage of this model is that its parameters can be identified more reliably from distinct fed-batch strategies, such as glycerol pulses and exponential followed by constant substrate additions. Interestingly, with the limited amount of data available, the proposed model accomplishes satisfactorily the experimental results obtained for the three state variables, and no exhaustive process knowledge is required. The comparison of the measurement data obtained in a validation experiment with the model predictions showed the great extrapolation capability of the model presented, which could provide new complementary information for the COMT production system.

• 생명과학회지
• /
• 제17권11호
• /
• pp.1582-1586
• /
• 2007

균주 JH232가 생성한 bacterial cellulose (BC)는 이온교환 막 생산을 위한 환경친화적 재료로서 잠재능이 있다는 것이 전 연구(J. of Chem. Technol. Biotechnol. 2004, 79, 79-84)를 통해 보고되었다. 본 연구를 통해 JH232를 동정하였으며 BC 생성 특성을 조사하였다. 16S rRNA 유전자의 비교분석을 통해 JH232는 Gluconacetobacter sp.인 것을 밝혀냈다. 플라스크실험 결과 이 균주는 초기 pH 5.5로 조절된 CSL 배지에서 온도가 $30^{\circ}C$인 조건하에 배양하였을 때 BC 생성량이 최대를 나타내었다. 발효조 실험을 통해서 회분식 배양보다 유가배양에 의해 JH232의 BC 생성량이 1.56배 더 높게 나타났다.

• 상하수도학회지
• /
• 제8권3호
• /
• pp.1-8
• /
• 1994

In the general process of design for aerobic digestion, the design for field plant of which inflow pattern is continuous inflow is performed using the results from lab scale batch reactor. However, the recent researchers reported that the general designs were performed as over-estimated, Therefore, in this study, laboratory batch experiments were carried out at $20^{\circ}C$ and pH 7.5 on the aerobic digestion of waste activated sludge at different solid levels. This treatise could consider the negligence about effective digestion periods the usage of VSS as solid concentration, and the effect of initial solid concentration of solid degration rate coefficient($k_d$) as reasons of the overestimated design, and showed the scheme of how to design for aerobic digestion from batch experiment.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 1996년도 Proceedings of the Korea Automatic Control Conference, 11th (KACC); Pohang, Korea; 24-26 Oct. 1996
• /
• pp.263-266
• /
• 1996

A method to determine an optimal temperature trajectory that guarantees polymer products having controlled molecular weight distribution and desired values of molecular weight is presented. The coordinate transformation method and the optimal control theory are applied to a batch PMMA polymerization system to calculate the optimal temperature trajectory. Coordinate transformation method converts the original fixed-end-point, free-end-time problem to a free-end-point, fixed-end-time problem. The idea is that by making the reactor temperature track the optimal temperature trajectory one may be able to produce polymer products having the prespecified physical property in a minimum time. The on-line control experiments with the PID control algorithm have been conducted to establish the validity of the scheme proposed in this study. The experimental results show that prespecified polymer product could be obtained with tracking the calculated optimal temperature trajectory.

• 한국표면공학회지
• /
• 제25권3호
• /
• pp.117-125
• /
• 1992

In the electrolysis process of zinc plating impurity ions must be removed from zinc chloride plating solution because it's harmful to the current efficiency and the purity of zinc plating. In this study using zinc ball as a prcipitant instead of zinc dust, the fundamental data for continuous cementation process was studied. Based upon two series of experiments that consist of batch experiment with cylindrical zinc specimen and continuous experiment with zinc balls, following results have been obtained. In the continuous experiment all impurity ions have been removed within 1 hour regardless of various experimental conditions.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
• /
• 제8권4호
• /
• pp.79-86
• /
• 2019

본 논문에서 인공 신경망을 이용한 시계열 데이터 예측 사례에 대해 서술한다. 본 연구에서는 텐서 플로우 라이브러리를 사용하여 배치 기반의 인공 신경망과 스타케스틱 기반의 인공신경망을 구현하였다. 실험을 통해, 구현된 각 신경망에 대해 훈련 에러와 시험에러를 측정하였다. 신경망 훈련과 시험을 위해서 미국의 인디아나주의 공식 웹사이트로부터 8개월간 수집된 세금 데이터를 사용하였다. 실험 결과, 배치 기반의 신경망 기법이 스타케스틱 기법보다 좋은 성능을 보였다. 또한, 좋은 성능을 보인 배치 기반의 신경망을 이용하여 약 7개월 간 종합 세수 예측을 수행하고 예측된 결과와 실제 데이터를 수집하여 비교 실험을 진행 하였다. 실험 결과, 예측된 종합 세수 금액 결과가 실제값과 거의 유사하게 측정되었다.

• 대한환경공학회지
• /
• 제39권9호
• /
• pp.505-512
• /
• 2017

NaA 제올라이트 분말(Z-PA) 및 펠렛(Z-BA)를 이용하여 수중의 Sr 이온의 흡착특성을 검토하였다. 회분식 실험에서 Z-BA와 Z-PA에 의한 Sr 이온의 흡착은 유사 1차식보다는 유사 2차식에 잘 부합되었고, 등온 흡착실험 결과는 Langmuir 등온식에 잘 부합하였으며, 이 등온식으로부터 구한 최대 흡착량은 Z-PA는 233.32 mg/g, Z-BA는 164.60 mg/g이었다. 연속식 실험에서 Sr 이온의 농도가 증가함에 따라 Sr 이온의 총 흡착량(q)는 증가하였으나 파과시간, 처리 부피($V_{eff}$) 및 총 제거율(R)은 감소하였다. 실험으로부터 구한 파과곡선은 Thomas 모델식에 의해 잘 모사되었다.

• 상하수도학회지
• /
• 제20권1호
• /
• pp.156-163
• /
• 2006

While sludge is settling in batch column, sludge concentration becomes high. Because the characteristic of sludge settling changes in function of time due to the sludge concentration change, the sludge settling velocity changes too. Also, because the sludge settling characteristic is influenced by a physical characteristic of sludge and a column height etc, it is difficult to exactly measure the sludge settling characteristic. Although the sludge volume indexes, SVI, SSVI and $SSVI_{3.5}$, are used to predict sludge settling characteristic, these indexes are not reliable values. Because the previously established models for sludge settling velocity predict the sludge settling velocity only, it is difficult to predict sluge-water interface height by using those models. The purpose of this experiment is to establish the empirical model which predicts the sludge interface height change with respect to the sludge physical characteristic and the settling condition.

• Ocean and Polar Research
• /
• 제25권3호
• /
• pp.269-275
• /
• 2003

Effects of different operating factors including superficial air velocity (SAV), hydraulic residence time (HRT), protein concentration, and foam overflow height on protein removal by a foam fractionator in simulated seawater aquaculture system were investigated. This experiment was conducted on batch and consecutive modes at different combinations of the affecting factors. The foam fractionator had a diameter of 20cm and a height of 120cm and the experiment was conducted with synthetic wastewater. In 5 consecutive trials, protein concentrations in culture tank water decreased faster when the foam fractionator was operated at higher SAVs and lower HRTs. In batch trials, protein removal rates increased with an increase in SAV but decreased with an increase in URT. Higher protein concentrations in the bulk solution resulted in higher protein removal rates. Protein concentrations in the collected foam condensates increased but the foam overflow rates decreased with the increase of foam overflow heights. The results of this experiment indicate that foam fractionation would be an effective way for protein removal in seawater aquaculture systems and the performance of the foam fractionator depends largely on the operating parameters, especially SAV.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제17권6호
• /
• pp.92-101
• /
• 2012

Korea shows the soil pH is 5.8 ~ 6.2 by many factors including the geological structure and climate condition. There is known as the cause for soil acidification by weathering of the mineral, excessive use of the chemical fertilizer, and extensively diffused acid rain. The purpose of research is environmentally-friendly material neutralization technology development utilizing the waste resources against by acid soil. The experiment analyze the physico-chemical property of the acid soil and waste resource materials (waste lime, oyster shell, bottom ash). The Batch-Test was performed under 3 stage. As a result, the acid soil showed up acid soil about 3.19. And waste lime, oyster, bottom ash showed the alkalinity with 9.62, 10.08, 9.17. In case of 1 batch-test experimental result, waste lime and oyster shell, the alkalinity was shown over 7.5 and the good efficiency was showed, on the other hands, the bottom ash showed the pH 4 the neutralization efficiency which is low. waste resource materials to be applied to 2 steps was chosen as the waste lime except the bottom ash and oyster. In 2 step batch-test experiment, it was exposed to be the most appropriate in case of doing the combination ratio of the waste lime and oyster shell with 9 : 1. It was exposed to be efficient most in the effeciency and aspect of economical efficiency combination ratio of the soil and materials was 9.6 : 0.6 with 3 step batch-test experimental result.