• 대한환경공학회지
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• 제36권10호
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• pp.704-710
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• 2014

바이오매스는 지구온난화에 중요한 기여자인 이산화탄소와 같은 온실가스를 해결할 수 있는 대체에너지로 간주된다. 또한 바이오매스 에너지는 열화학적 전환 공정을 통해 다양한 형태로 전환된다. 본 연구에서는 목질계 바이오매스의 가스화를 위해 연속식 가스화기를 제작하였다. 목질계 바이오매스는 폐목재를 사용하였다. 이산화탄소 가스화 실험은 가스화 온도, 함수율 그리고 주입 이산화탄소 농도 변화에 따라 진행하였다. 실험결과는 가스화 온도가 증가함에 따라 생성가스 발생량이 증가함을 보였다. 경질타르는 중질타르의 열적 분해에 의해 증가되었고, 주사현미경 분석을 통해 촤 세공형성이 발달되는 것을 확인하였다. 일산화탄소 농도는 부다 반응에 의해 이산화탄소 주입농도 증가함에 따라 증가하였다. 변수별 실험에 의해, 최적 실험 조건에서 수소와 일산화탄소는 32.91%와 48.33%가 생성되었다.

• Journal of the Korean Wood Science and Technology
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• 제48권5호
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• pp.651-665
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• 2020

Hydrolysis of biomass for the production of fermentable sugar can be improved by the addition of surfactants. In pulp and paper mills, lignin, which is a by-product of the pulping process, can be utilized as a fine chemical. In the hydrolysis process, lignin is one of the major inhibitors of the enzymatic breakdown cellulose into sugar monomer. Therefore, the conversion of lignin into a biosurfactant offers the opportunity to solve the waste problem and improve hydrolysis efficiency. In this study, lignin derivatives, a biosurfactant, was applied to enzymatic hydrolysis of various lignocellulosic biomass. This Biosurfactant can be prepared by reacting lignin with a hydrophilic polymer such as polyethylene glycol diglycidylethers (PEDGE). In this study, the effect of biosurfactants on the enzymatic hydrolysis of pretreated sweet sorghum bagasse (SSB), oil palm empty fruit bunch, and sugarcane trash with different lignin contents was investigated. The results show that lignin derivatives improve the enzymatic hydrolysis of the pretreated biomass with low lignin content, however, it has less influence on the enzymatic hydrolysis of other pretreated biomass with lignin content higher than 10% (w/w). The use of biosurfactant on SSB kraft pulp can increase the sugar yield from 45.57% to 81.49%.

• Journal of the Korean Wood Science and Technology
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• 제51권3호
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• pp.207-221
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• 2023

The increasing demand for clean energy requires considerable effort to find alternative energy sources, such as briquettes. This research aims to develop a charcoal briquette with added pine resin (API) that has excellent combustion speed and distinctive aroma. Briquettes are composed of charcoal, pine resin (concentration: 0%-30%), and starch (up to 7%). They are produced in several stages, including coconut shell pyrolysis in conventional combustion, to obtain charcoal for the briquette precursor. Briquette compaction is conducted by mixing and densifying the charcoal, pine resin, and starch using a hydraulic press for 3 min. The hydraulic press has a total surface area and diameter of 57.7 cm² and 3.5 cm, respectively. The briquettes are dried at different temperatures, reaching 70℃ for 24 h. The study results show that the briquettes have a thickness and diameter of up to 2 and 3.5 cm, respectively; moisture of 2.18%-2.62%; ash of 11.61%-13.98%; volatile matter of 27.15%-51.74%; and fixed carbon content of 40.24%-59.46%. The compressive strength of the briquettes is 186-540 kg/cm². Their calorific value is 5,338-6,120 kcal/kg, combusting at a high speed of 0.15-0.40 s. The methoxy naphthalene, phenol, benzopyrrole, and lauryl alcohol; ocimene, valencene, and cembrene are found in the API. The API briquette has several chemical compounds, such as musk ambrette, ocimene, sabinene, limonene, 1-(p-cumenyl) adamantane, butane, and propanal, which improve aroma, drug application, and fuel production. Accordingly, API briquettes have considerable potential as an alternative energy source and a health improvement product.

• 한국토양비료학회지
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• 제47권6호
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• pp.486-490
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• 2014

This study was conducted to investigate the effect of carbonized biomass from crop residues on soil carbon storage during soybean cultivation. The carbonized biomass was made by field scale mobile pyrolyzer. The treatments consisted of control without input and three levels of carbonized biomass inputs as $59.5kg10a^{-1}$, C-1 ; $119kg10a^{-1}$, C-2 ; $238kg10a^{-1}$, C-3. Soil samples were collected during the 113 days of experimental periods, and analyzed soil pH and moisture contents. Soil carbon contents and soybean yield were measured at harvesting period. For the experimental results, soil pH ranged from 6.8 to 7.5, and then increased with increasing carbonized material input. Soil moisture contents were slightly higher by 0.1~1.5% than the control, but consistent pattern was not observed among the treatments. Soil carbon and organic carbon contents in the treatments increased at 24 and 15% relative to the control at 15 days after sowing, respectively. Loss rate of SOC (soil organic carbon) relative to its initial content was 7.2% in control followed by C-1, 6.8%> C-2, 3.5%>C-3, 1.1% during the experimental periods. The SOC change rate decreased with increasing carbonized biomass rate. It was appeared that soybean yields were $476.9kg10a^{-1}$ in the control, and ranged from 453.6 to $527.3kg10a^{-1}$ in the treatments. However, significant difference was not found among the treatments. It might be considered that the experimental results will be applied to soil carbon sequestration for future study.

• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.254-264
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• 2010

To obtain an efficient natural lignocellulolytic complex enzyme, we screened an efficient lignocellulose-degrading composite microbial system (XDC-2) from composted agricultural and animal wastes amended soil following a long-term directed acclimation. Not only could the XDC-2 degrade natural lignocelluloses, but it could also secrete extracellular xylanase efficiently in liquid culture under static conditions at room temperature. The XDC-2 degraded rice straw by 60.3% after fermentation for 15 days. Hemicelluloses were decomposed effectively, whereas the extracellular xylanase activity was dominant with an activity of 8.357 U/ml on day 6 of the fermentation period. The extracellular crude enzyme noticeably hydrolyzed natural lignocelluloses. The optimum temperature and pH for the xylanase activity were $40^{\circ}C$ and 6.0. However, the xylanase was activated in a wide pH range of 3.0-10.0, and retained more than 80% of its activity at $25-35^{\circ}C$ and pH 5.0-8.0 after three days of incubation in liquid culture under static conditions. PCR-DGGE analysis of successive subcultures indicated that the XDC-2 was structurally stable over long-term restricted and directed cultivation. Analysis of the 168 rRNA gene clone library showed that the XDC-2 was mainly composed of mesophilic bacteria related to the genera Clostridium, Bacteroides, Alcaligenes, Pseudomonas, etc. Our results offer a new approach to exploring efficient lignocellulolytic enzymes by constructing a high-performance composite microbial system with synergistic complex enzymes.

• Journal of Plant Biology
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• 제38권4호
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• pp.389-398
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• 1995

한국 서해 중부 연안의 12개 지소에서 조간대 해조류의 종조성과 생물량을 조사하였다. 조사지역에서 채집 동정된 해조류는 남조식물 19종, 녹조식물 20종, 갈조식물 32종 및 홍조식물 80종으로 총 151종이었다. 이 가운데 지충이(Sargassum thunbergii), 마디털(Stylonema alsidii) 및 꼬시래기(Gracilaria verrucosa)의 3종이 12개 조사지소 모두에서 출현하였다. 단위면적당 해조류 생물량은 백사장과 대천에서 41 g dry $wt{\cdot}m^{-2}$의 매우 낮은 평균 생물량을 보인 반면, 조사지역 가운데 가장 북쪽에 위치한 의항리에서 549 g dry $wt{\cdot}m^{-2}$로 가장 많았는데, 이 수준은 지금까지 서해안에서 측정된 해조류 생물량 가운데 가장 많은 양이다. 해조류의 문별 생물량 구성비율은 전반적으로 갈조식물이 가장 높게 나타난 반면, 녹조식물이 가장 낮았다. 출현종 가운데 지충이는 모든 조사지소에서 조간대 전반에 걸쳐 우점적인 생육을 보였다. 그러나 산호말류(Corallina spp.)는 조사지역의 상부인 태안반도의 지소들에서 우점적인 생육을 보였고 하부의 지소들에서도 부분적으로 출현하였으나, 중부의 지소들에서는 거의 출현하지 않는 분리 분포를 보였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권4호
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• pp.351-356
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• 2006

This study developed an artificial neural network (ANN) to estimate the growth of microorganisms during a fermentation process. The ANN relies solely on the cumulative consumption of alkali and the buffer capacity, which were measured on-line from the on/off control signal and pH values through automatic pH control. The two input variables were monitored on-line from a series of different batch cultivations and used to train the ANN to estimate biomass. The ANN was refined by optimizing the network structure and by adopting various algorithms for its training. The software estimator successfully generated growth profiles that showed good agreement with the measured biomass of separate batch cultures carried out between at 25 and $35^{\circ}C$.

• ALGAE
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• 제19권2호
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• pp.149-151
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• 2004

Freshwater algae make up a very important portion of the autotrophic component of the aquatic food web. Therefore, the study of freshwater algal structure and biomass is central to aquatic ecosystem studies. Due to variations in cell shape and size for each species (or taxon) and survey site, cell abundance (or cell numbers per chosen volume) often leads to misrepresentation of the true importance of some species because of the great differences in size of various algae. Thus, it is necessary to investigate the freshwater algal species of a site in order to calculate the cell volume. Although direct cell counting, species volume measurement, as well as biomass calculation are time-consuming and requiring specialists in taxonomy.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제2권1호
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• pp.21-28
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• 1998

A total of 94 taxa were identified from the Southern Ocean, 140-148 $^{\circ}E$ and 40-53 $^{\circ}S$, as an early austral summer phytoplankton. They were 53 diatoms, 37 dinoflagellates, 2 silicoflagellates, 1 prymnesiophyte, and 1 coccolithophorid. Integrated cell numbers of nanoplankton dominated microphytoplankton from 8 stations, especially from Subantarctic zone, but integrated biomass was lower than microphytoplankton. Integrated cell numbers of diatoms dominated dinoflagellates, coccolithophorids, and prymnesiophyte, but integrated biomass of microphytoplankton were dependent to the biomass of dinoflagellates except north of the Subtropical convergence zone and south of the Antractic convergence zone. Phytoplankton community changed across the fronts and 3 different communities were observed. Fronts seem to influence on the phytoplankton community from the west Pacific Sector of the Southern Ocean.

• Journal of Microbiology and Biotechnology
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• 제5권2호
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• pp.114-116
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• 1995

Concentrated Brazillian sugar cane stillage was used as a substrate for the yeast biomass production using Candida rugosa isolated from East Africa. At the optimum stillage concentration of 10% dry matter, biomass production was 20.4 g/l and COD reduction rate was 41%. The specific growth rate of the yeast was 0.17 $h^{-1}$ and the corresponding productivity 0.91 g $l{-1} h^{-1}$ in the batch fermentation was observed at $40{\circ}^C$.