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The Effect on the Change of Liver and Serum Cholesterol Contents of Albino Rats by the Feeding of the Suerose and Glucose (蔗糖과 葡萄糖이 白鼠肝臟 및 血淸 Cholesterol의 含量에 미치는 影響)

  • Koh, Jin Bog;Lee, Kyung Ro
    • The Korean Journal of Zoology
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    • v.13 no.2
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    • pp.44-50
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    • 1970
  • This experiment was made in order to observe the change in contents of the total cholesterol, free cholesterol and ester cholesterol in the liver and serum by the feeding of glucose and sucrose. The animals used for the experiment were adult male albino rat from a pure strain, weighing 285-332g. The animals were divided into standard, glucose and sucrose diet groups. The glucose and sucrose diet groups were redivided into 10%, 25% and 40% diet groups. Their liver and serum were used as sample after they were fed with the corresponding diets, respectively, for two months. 1. In the liver, the total cholesterol and ester cholesterol contents in the 40% glucose diet group were significantly increased and the free cholesterol contents in all diets were respectively increased, compared with the standard diet group. 2. In the serum, the total cholesterol contents in both diet groups, the 10% glucose and 10% sucrose, were decreased but the content in the 40% sucrose diet group was increased, compared with the standard diet group. The free cholesterol contents in the 10 and 40% glucose diet and 10% sucrose diet group were decreased, compared with the standard diet group. The ester cholesterol contents in the 10% glucose and 10% sucrose diet groups were decreased but the content in the 40% sucrose diet group was increased, compared with the standard diet group. Being taken into consideration of the above facts it was acquired that the cholesterol contents are affected by the amounts and kinds of dietary carbohydrate when the protein contents in each diet were constant.

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A study on the electrolytic properties of $CaF_2$ crystals with $YF_3$ addition ($YF_3 $ 첨가에 따른 $CaF_2 $ 결정의 고체전해질 특성에 관한 연구)

  • Cha, Y.W.;Park, D.C.;Orr, K.K.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.4 no.1
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    • pp.21-32
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    • 1994
  • $CaF_2$ crystals were grown with various growth rates by Bridgman method, and the electrical properties of these were studied to examine the changes of ionic conductivities with growth rates by AC Impedance Analyzer. As the growth rates were higher, $CaF_2$ crystals were grown to polycrystals from single crystal. And as grain boundaries and various defects were altered, the ionic conductivities were changed dramatically. $YF_3$ added to $CaF_2$ for disorderizing $CaF_2$ structure and improving the number of $F^-$ carriers and vacancies in $CaF_2$ crystals. Then $Ca_{1-x}Y_XF_{2+X}$ crystals were gained. And the ionic conductivities of $Ca_{1-x}Y_XF_{2+X}$ crystals were investigated with $YF_3$ addition. The ionic conductivities of $CaF_2$ and $Ca_{1-x}Y_XF_{2+X}$ crystals with temperatures were compared. In addition, the effects of clusterings and defects on the electrical properties of solid electrolytes were researched.

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Histological and Histochemical Studies on the Mucous Secreting Cells of the Gastric Mucosae according to the Development of Frog, Rana nigromaculata (개구리(Rana nigromaculata) 발생에 따른 위점막 점액분비세포의 조직학적 및 조직화학적 연구)

  • Kim, Han-Hwa;Noh, Yong-Tai;Chung, Young-Wha
    • The Korean Journal of Zoology
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    • v.19 no.1
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    • pp.25-32
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    • 1976
  • To observe the changes of mucosubstances of the mucous secreting cells, stomach tissues in frog tadpoles at each stage of metamorphosis were fixed in 10% buffered formalin at $4^{\circ}C$, embedded in paraffin, sectioned to 4 $\mu$m thickness and stained with periodic acid-Schiff(PAS) and alcian blue (AB) of pH 2.5 and pH 1.0. The results obtained were as follows: 1. The reactivities of the surface mucous cells, which exhibited strong PAS-positivity and weak alcianophilia at both pH 2.5 and 1.0, were not changed in metamorphosis stages and the intracellular contents of neutral mucosubstances in the surface mucous cells increased significantly in XXIV and XXV stages of metamorphosis. 2. In the foveolar mucous cells, which appear after metamorphosis XXI, the staining reactivities to PAS, AB of pH 2.5 and 1.0 were the same as that of surface mucous cells during metamorphosis and the alcianophilia were stronger at pH 1.0 than at pH 2.5. 3. THe mucous neck cells, which appear after metamorphosis XXIV, exhibited a strong PAS-positive reaction and weak alcianophilia at metamorphosis XXIV but at metamorphosis XXV weak reactivity to PAS and strong alcianophilia at pH 1.0.

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A Study on electrophoresis and Activity of Alcohol Dehydrogenase of Drosophila melanogaster (한국산 노랑초파리 (Drosophila melanogaster) Alcohol dehydrogenase의 전기영동산 및 활성에 대한 연구)

  • Chung, Yong-Jai;Yoon, Youn-Sik
    • The Korean Journal of Zoology
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    • v.23 no.3
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    • pp.125-136
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    • 1980
  • The purpose of the present study is to investigate the electrophoretic patterns of ADH isozymes and activity at various developmental stages of Oregon-R and Kwangju strains of D. melanogaster. The ADH isozymes were seperated by agarose gel plate eletrophoresis and they were stained for ADH activity study. The optical density was measured by using densitometer and the total activity was determined by measuring the enzyme solution's absorbance of 340 nm by spectrophotometer. The results are as follows: 1. At egg stages of Oregon-R and Kwangju strains ADH 2, ADH 3, ADH 4 were observed in te zymograms, but all of ADH 1, ADH 2, ADH 3, ADH 4 and ADH 5 were detected in larval, pupal and imaginal stages. 2. The amount of ADH 1+ADH 2 (anion) was 20-40% and that of ADH 3+ADH 4+ADH 5 (cation) was 60-805. In the developmental stages other, than egg stages, ADH 3 of both strains showed the largest amount among all ADH isozymes, and especially ADH 4 of Kwangju strain showed less amount than that of Oregon-R strain. 3. The total activity of the ADH at the egg stage was found to be the lowest, at larval stage became to be higher and to be lower at pupal stage and then became to be the highest at the imaginal stage. It is noticeable that the activity of the ADH in Kwangju strain was higher than that in Oregon-R strain.

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Studio sulla regia di Strehler -Sulla regia de I giganti della montagna- (스트렐러의 연출론 연구 - I giganti della montagna 의 시대별 연출 -)

  • Jang, Ji Yeon
    • Lettere Italiane
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    • no.27
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    • pp.155-169
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    • 2009
  • Al centro dell'interesse di Strehler ci sono sempre l'uomo e le sue azioni, e l'uomo e la posizione del teatro. Inoltre, nei suoi teatri vengono alla luce i rapporti che interessano l'uomo e la societa', l'uomo e se stesso, l'uomo e la storia, l'uomo e la politica. Strehler interpreta sempre i testi prendendo in considerazione la contemporaneita' e completa la rappresentazione strutturandola in un sistema implacabilmente coerente di segni scrulolosi. Poiche' le sue opere, anche se le stesse, sono rappresentate ogni volta con significati diversi a secondo del tempo, non si tratta soltanto di rivivere le opere, ma di metterle in scena in nuove versioni che hanno significati che interpretano i cambiamenti avvenuti. Questo e' anche il caso de I giganti della montagna. Nella prima regia di quest'opera, Strehler cerca di mettere in evidenza una concreta e generale storia umana attraverso il mito, e un messaggio che è al tempo stesso di disperazione e di fede nell'uomo. Un messaggio di perdono e di pietà. Pietà per tutti. Nella seconda regia, egli incarna, attraverso il vuoto dell'immobilita' e il silenzio, la vita della poesia. Cerca di rappresentare l'estremo degli effetti scenici attraverso la minima mobilita' in una scena immobile, e attraverso il massimo grido di dolore in una scena di silenzio. E anche i personaggi sono divisi in due diverse categorie. 'Gli scalognati', che creano 'teatro puro' non per un pubblico ma per puro giuoco, e 'la compagnia dei comici', che crea 'teatro rappresentato' che implica la possibilita' dell'interprete condizionato dai rischi, dagli errori di valutazione e d'interpretazione e che resta legato ad una realta' concreta. Attraverso questo contrasto egli vuole esprimere "il timore che nonostante tanta "rivoluzione" giovanile intorno, il cammino della societa' degli uomini verso l'abbandono di alti ideali e valori, fosse accelerato e quasi ineluttabile". Nella terza regia, i due mondi si incontrano in uno spazio dove realta' e sogno si confondono. Non sono piu' contrapposti. E' come "una tragica contestazione che i Giganti hanno vinto e che ci hanno, consapevoli o inconsapevoli, travolti, che non siamo piu gli stessi, che stiamo precipitando". Ma egli non dispera, benche' rappresenti la condizione tragica, dice al pubblico di "comunicare in silenzio la reciprocita' delle sconfitte. al di la' delle parole che non esistono".

Effect of Oral Health Care on Swallowing Function and Swallowing-Quality of Life in Patients with Dementia (치매환자의 구강관리가 삼킴기능과 구강관련 삶의 질에 미치는 영향)

  • Bae, Seong-Hwan;Baek, Ji-Young
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.7
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    • pp.449-459
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    • 2019
  • The purpose of this study was to evaluate the status of oral health care in dementia patients living in long-term care facilities and to investigate the effects of this on swallowing function and swallowing-quality of life. The subjects were 60 demented patients who were admitted to long-term care facilities in Gwangju and Jeonnam provinces. Their Oral Health Assessment Tool(OHAT), Clinical Dysphagia Scale(CDS), and Oral Health Impact Profile 14(OHIP-14) were measured. The results of this study were as follows: First, the effect of oral health care on dentition patients using long-term care facilities on swallowing function and swallowing-quality of life was significantly different according to oral care score (p<.05)(p<.01). Second, the relationship between oral health care and swallowing function of dementia patients and swallowing-quality of life. was examined. As a result, oral health care of dementia patients was affected by swallowing function (r=.405, p<.01) the swallowing-quality of life (r=.462, p<.01) and swallowing function were significantly correlated with swallowing-quality of life (r=.351, p<.01). Therefore, the oral health care of users of long-term care facilities was closely related to swallowing function and swallowing-quality of life.

Learning Experiences of the Project Approach in Early Childhood Preservice Teachers (예비유아교사가 경험한 프로젝트 접근법)

  • Yang, Jung-Eun
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.8
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    • pp.457-467
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    • 2019
  • The purpose of this study is to find out how those taking early childhood preservice teachers experience their learning through learner-centered education and the meaning given by such experiences to grasp their values. Especially, to look into experiences of learning the project approach theory studied and directly experienced, which is one of the early childhood educational approach methods, data on participatory observation, personal interviews, personal reflective journals, activity reports and ect. were collected and analyzed by the current writer, who fully participated in the class. The meaning of early childhood preservice teacher'experiences in the project approach was analyzed from the two aspects of 'my' learning activities and 'teacher's teaching activities. In the aspect of 'my' learning activities, they got indulged through purposeful practices in the process of tackling problems that originated from personal interest and wanted to share the joy of learning they'd had with others. Aware of themselves as doers of behavior and thinking, they were found to be able to focus an 'my' own learning activities based on these learning experiences of theirs. In the aspect of 'teacher's teaching activities, project theories and practices done at the same time helped them internalize the project and they learned possible changes ign the project by experiencing actual cases. To sum up, the project approach has its meaning in that it not only helps teachers learn theoretical knowledge but also have reflective thinking through their experiences as doers of learning and form practical knowledge. Accordingly, it indicates th need for intensive discussion on the project approach as a way to educate pre-teachers or current ones.

MicroRNA analysis reveals the role of miR-214 in duck adipocyte differentiation

  • Wang, Laidi;Hu, Xiaodan;Wang, Shasha;Yuan, Chunyou;Wang, Zhixiu;Chang, Guobin;Chen, Guohong
    • Animal Bioscience
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    • v.35 no.9
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    • pp.1327-1339
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    • 2022
  • Objective: Fat deposition in poultry is an important factor in production performance and meat quality research. miRNAs also play important roles in regulating adipocyte differentiation process. This study was to investigate the expression patterns of miRNAs in duck adipocytes after differentiation and explore the role of miR-214 in regulating carnitine palmitoyltransferases 2 (CPT2) gene expression during duck adipocyte differentiation. Methods: Successful systems for the isolation, culture, and induction of duck primary fat cells was developed in the experiment. Using Illumina next-generation sequencing, the miRNAs libraries of duck adipocytes were established. miRanda was used to predict differentially expressed (DE) miRNAs and their target genes. The expression patterns of miR-214 and CPT2 during the differentiation were verified by quantitative real-time polymerase chain reaction and western blot. Luciferase reporter assays were used to explore the specific regions of CPT2 targeted by miR-214. We used a miR-214 over-expression strategy in vitro to further investigate its effect on differentiation process and CPT2 gene transcription. Results: There were 481 miRNAs identified in duck adipocytes, included 57 DE miRNA candidates. And the 1,046 targets genes of DE miRNAs were mainly involved in p53 signaling, FoxO signaling, and fatty acid metabolism pathways. miR-214 and CPT2 showed contrasting expression patterns before and after differentiation, and they were selected for further research. The expression of miR-214 was decreased during the first 3 days of duck adipocytes differentiation, and then increased, while the expression of CPT2 increased both in the transcriptional and protein level. The luciferase assay suggested that miR-214 targets the 3'untranslated region of CPT2. Overexpression of miR-214 not only promoted the formation of lipid droplets but also decreased the protein abundance of CPT2. Conclusion: Current study reports the expression profile of miRNAs in duck adipocytes differentiated for 4 days. And miR-214 has been proved to have the regulator potential for fat deposition in duck.

A Study on the Differences in Senior Fitness Levels for Health Life in the Elderly Women (건강을 위한 여성고령자의 노인체력수준 차이에 관한 연구)

  • Ahn, Yong-Duk;Shin, Jeong-Hun
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.8
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    • pp.367-375
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    • 2019
  • The purpose of this study is to help the elderly women's health improvement by comparing body composition factors and differences in senior fitness levels for health life according to the degree of obesity and physical activity levels in the elderly women. The subjects of the study were 32 elderly women and their consent to the study was obtained. The degree of obesity was classified by measuring body composition and physical activity levels were classified based on the questionnaire survey. Body composition factors and senior fitness levels were compared. Finally, the following conclusions were drawn. 1. The body composition factors according to the classification of the degree of obesity were significantly different in high blood pressure. Senior fitness was significantly different in 30-Second Chair Stand, Arm Curl, 2min Step Test, 8 Foot up and go. High degree of obesity was associated with decreased 30-Second Chair Stand, Arm Curl and 2min Step Test for senior fitness. 2. The body composition factors according to physical activity levels were significantly different in high blood pressure. Senior fitness was significantly different in 30-Second Chair Stand, Arm Curl, Chair Sit and Reach, 2min Step Test, 8 Foot up and go. High physical activity was associated with increased 30-Second Chair Stand, Arm Curl, Chair Sit and Reach, 2min Step Test, 8 Foot up and go for senior fitness.

The Effect of General Eduction Class on Skin Care Based on Service Learning on Quality of Life and Self-Efficacy (봉사학습 기반의 대학 피부미용 교양수업이 삶의 질, 자기효능감에 미치는 영향)

  • Park, Jeong-Yeon;Sim, Hyeon-Ae
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.7
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    • pp.479-485
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    • 2019
  • The purpose of this study was to explore practical direction of university general education through considering the goal and value by analyzing the effect of learning in students participating in general eduction class on skin care based on service learning. The subjects were 30 students in which female (n=24; 80%) and Juniors (n=16; 53.3%) are most common. Their most common major and religion were humanities and social sciences (n=12; 40.0%) and no-religion (n=21; 70%), respectively. The difference between pre- and post-service learning was analyzed to determine the effect of the service learning on the self-efficacy and the quality of life and the results showed significant difference, indicating the positive effect of service learning in improving the self-efficacy and the quality of life. The score of satisfaction with service learning was found to be 4.12, indicating high satisfaction and the intention to retain service learning was found to be 3.99, indicating they have the intention to retain service learning. In addition, there was no difference in the satisfaction with and the intention to retain service learning depending on the characteristics such as gender, grade, major, and religion. These results showed that the general eduction class on skin care based on service learning had positive effect on the self-efficacy and quality of life of learners. There effects are expected to helpful, through university education in developing a sense of community and strengthening social responsibility of learners as social identities.