• Korean Journal of Ichthyology
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• v.20 no.1
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• pp.1-6
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• 2008

Three types of clonal cell lines were isolated according to their size and phenotype from the adherent cell populations in long-term liquid cultures from the embryonic fibroblast cells of Zebrafish, Danio rerio. All kind of cell lines were well proliferated. The size and number of clonal cell lines derived colonies from stable embryonic cells were significantly increased in the presence of NAC and A2P conditioned medium from the cell lines. The stable cell lines and clonal cell lines were cap-able of well proliferation in vitro. These cell lines have been maintained in continuous culture without change in characteristics. A majority of the clonal cells (80%) was shown a normal chromosomal complement (50 chromosomes, 2N) in according with FACs analysis. Majority of cells were positive to vimentin staining and none of them were positive for nestin and Oct -4 by immunocytochemistry. These results indicate that the clonal cell lines obtained from cultured cells are fibroblasts and may be extremely useful in genetic manipulation for further nuclear transfer and fish cloning.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1996.04a
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• pp.369-372
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• 1996

The factory under this study consists of mixed-model assembly lines and workcenters which provide parts to the main lines. Parts produced by the workcenter have different specifications for different product models. The workcenters fabricate parts in batches, and they are divided into two types. A type 1 center supplies parts only to the main line that is designated to the center while type 2 center provides parts to all the main lines. The purpose of this study is to develop a scheduling scheme for the workcenter, and the main objective of the schedules is to provide parts for the main lines without delay. The facts that make the scheduling challengeable are that 1) the different models existing together on a main line request different parts, 2) the spaces for part inventories are limited and 3) set up times are sequence dependent and long in some cases. This study presents developed scheduling schemes for the type 1 center and explains the scheduling and control structure used.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2000.07a
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• pp.663-666
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• 2000

Laser direct writing of micro-patterned copper lines has been achieved by pyrolytic decomposition of copper formate films (Cu(HCOO)$_2$$.$4H$_2$O), as a precursor, using a focused Ar$\^$+/ laser beam ($\lambda$= 514 nm) on PCB boards and glass substrates. The linewidth and thickness of the lines were investigated as a function of laser power and scan speed. The profiles of the lines were measured by scanning electron microscope (SEM), surface profiler (${\alpha}$-step) and atomic force microscopy (AFM). The electrical resistivities of the patterned lines were also investigated as a function of laser parameter using probe station and semiconductor analyzer. we compared resistivities of the patterned lines with that of the Cu bulk, respectively.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.128-136
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• 2009

To obtain pure lines for breeding $F_1$ varieties of spreading surfinia and wave petunia, Petunia ${\times}$ hybrida, 20 lines of surfinia petunia and 28 lines of wave petunia that were considered uniform in growth and flowering characteristics were selected by self-pollination of the fifth($S_5$) or the seventh generation($S_7$). The 20 selected lines of surfinia petunia had the branch number ranged from 6.0 to 11.0 cm, and the internode length ranged from 2.0 to 4.2 cm. Among them, ten lines, including '$Pe99-017^7$' were above 60 cm of plant width, above 300 leaves in a plant. Fourteen lines including '$Pe99-017^7$' were more than 150 in the number of flower. In the petal color, thirteen lines, including '$Pe99-017^7$', were red-purple; three, including '$Pe99-007^7$', were purple; '$Pe04-086^7$' and '$Pe04-159^7$' were violet; and line '$Pe072-1^7$' was white. Eight lines including '$Pe02-205-2^5$' ranged from 4.0 to 5.0 cm of flowers diameter, and seven lines including '$Pe04-086^5$' ranged from 3.0 to 4.0 cm of leaf length, which is relatively low. Germination rate of the lines was more than 50%. In the wave petunia, the branch number of the 28 selected lines ranged from 8.0 to 13.0 cm, and the internode length ranged from 1.0 to 3.2 cm, which is relatively higher than surfinia petunias. Among them, ten lines, including '$Pe99-020^7$' were above 60 cm of plant width, above 200 leaves in a plant. Twelve lines including '$Pe04-034-2^5$' were more than 150 in the number of flower. In the petal color, eighteen lines, including '$Pe99-020^7$', were red-purple; three, including '$Pe04-113-4^5$', were red; three, including '$Pe04-263^5$', were white; '$Pe04-201^5$' and '$Pe04-263^5$' were violet blue; and line '$Pe04-072-5^5$' was purple. Nine lines including '$Pe04-201^5$' ranged from 4.0 to 5.0 cm of flowers diameter, and eleven lines including '$Pe04-263^5$' ranged from 3.0 to 4.0 cm of leaf length, which is relatively low. All the lines with various growth and flowering characteristics would be very promising to use as breeding materials for $F_1$ hybrids of spreading petunia, Petunia ${\times}$ hybrida.

• Journal of Advanced Navigation Technology
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• v.20 no.1
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• pp.59-64
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• 2016

This paper presents a high dividing ratio unequal dualband divider using coplanar waveguide (CPW) and shunt connected open stub transmission lines. In order to implement transmission lines for a dualband divider using design equations, the low impedance lines of divider can be realized a shunt connected open stub transmission line. Also, the high impedance lines are realized by CPW transmission lines. To certify the validity of an unequal dualband divider using CPW and shunt connected open stub transmission lines, the 10:1 unequal dualband divider is implemented at operating frequency of 1 and 2 GHz. Good experimental performance at each frequency are obtained, which are in good agreement with the simulated results.

• Journal of The Korean Astronomical Society
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• v.53 no.2
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• pp.35-42
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• 2020

We analyze high dispersion emission lines of the symbiotic nova AG Pegasi, observed in 1998, 2001, and 2002. The Hα and Hβ lines show three components, two narrow and one underlying broad line components, but most other lines, such as H_I, He_I, and He_II lines, show two blue- and red-shifted components only. A recent study by Lee & Hyung (2018) suggested that the double Gaussian lines emitted from a bipolar conical shell are likely to form Raman scattering lines observed in 1998. In this study, we show that the bipolar cone with an opening angle of 74°, which expands at a velocity of 70 km s^-1 along the polar axis of the white dwarf, can accommodate the observed double line profiles in 1998, 2001, and 2002. We conclude that the emission zone of the bipolar conical shell, which formed along the bipolar axis of the white dwarf due to the collimation by the accretion disk, is responsible for the double Gaussian profiles.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.247-252
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• 2002

The combination of radiation technique with an in vitro culture system was initiated to develop salt tolerant rice. We established an in vitro culture system to select tolerant lines against salt stress. NaCl tolerant cell lines were selected from the callus irradiated with gamma ray on N$_{6}$ medium with 1.5% NaCl and 2 mg/L 2,4-D. Regenerants (M$_1$) were obtained from the tolerant callus which was cultured for 30 days auxin-free medium. The M$_2$seeds were harvested from M$_1$plants on an individual plant basis. Thirty seedlings from each 450 M$_2$lines were transplanted in a field and total 5,000 M$_3$lines were harvested with an average 90 percent of fertile grain. M$_3$lines were utilized for selection of salt tolerance. Salinity-tolerant lines (225) were selected among 5,000 M$_3$lines. Of the 225 lines tested, the morphological traits of two lines (120-10 and -11) were far superior to control (Donagjinbyeo) in agromomic traits such as plant height, root length and no. of roots. Control and tolerant lines were analyzed by RAPD markers. Three polymorphic bands were presented in only tolerant lines, demonstrating a genetic difference between control and the tolerant lines. Such tolerant lines could be used as genetic resources to improve salt tolerance.e.

• BMB Reports
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• v.44 no.8
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• pp.523-528
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• 2011

To identify novel genes that are regulated by promoter methylation, a combinational approach involving in silico mining followed by molecular assay was performed. From the expression microarray data registered in the European bioinformatics institute (EBI), genes showing downregulation in breast cancer cells were initially screened and then selected by e-Northern analysis using the Unigene database. A series of these in silico methods identified CAMK2B and ARFGEF1 as candidates, and the two genes were revealed to be hypermethylated in breast cancer cell lines and hypomethylated in normal breast cell lines. Additionally, cancer cell lines showed downregulated expression of these genes. Furthermore, treatment of the cancer cell lines with a demethylation agent, 5-Aza-2'-deoxycytidine, recovered expression of CAMK2B and ARFGEF1, implying that hypermethyaltion silenced gene activity in cancer cells. Taken together, promoter methylations of CAMK2B and ARFGEF1 are novel epigenetic markers identified in breast cancer cell lines and can be utilized for the application to clinical cancer tissues.

• The Journal of Internal Korean Medicine
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• v.27 no.1
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• pp.166-177
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• 2006

Objectives : This study was performed to determine if Orostschys japonicus A. Berger has protective effects against CML in K562 cell lines. Materials and Methods : MTT assay, cell proliferation assay, Reverse transcription-polymerase reaction chain, RT-PCR, DNA fragmentation assay, Quantitative PCR were studied. Results : Orostschys japonicus A. Berger had no effects on Bax gene in K562 cell lines, but decreased Bcl-2 gene, and increased the Caspases-3 gene. This is indicate of induced apoptosis in K562 cell lines by Orostschys japonicus A. Berger. Conclusion : These results suggest that Orostschys japonicus A. Berger has effects on apatosis in K562 cell lines.

• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.205-211
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• 2006

A quantitative analytical method to detect new lines of genetically modified (GM) maize, NK603 and TC1507, has been developed by using a real-time polymerase chain reaction (PCR). To detect these GM lines, two specific primer pairs and probes were designed. A plasmid as a reference molecule was constructed from an endogenous DNA sequence of maize, a universal sequence of a cauliflower mosaic virus (CaMV) 35S promoter used in most GMOs, and each DNA sequence specific to the NK603 and TC1507 lines. For the validation of this method, the test samples of 0, 0.1, 0.5, 1.0, 3.0, 5.0, and 10.0% each of the NK603 and TC1507 GM maize were quantitated. At the 3.0% level, the biases (mean vs. true value) for the NK603 and TC1507 lines were 3.3% and 15.7%, respectively, and their relative standard deviations were 7.2% and 5.5%, respectively. These results indicate that the PCR method developed in this study can be used to quantitatively detect the NK603 and TC1507 lines of GM maize.