• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1903-1907
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• 2016

Background: Studies of effects of IL-1 polymorphisms, CYP2C19 genotype together with antibiotic resistance for H. pylori eradication are rare worldwide. The present study was designed to evaluate efficacy of 10-day sequential therapy (SQT) and 14-day standard triple therapy (STT) with four- times-daily dosing of amoxicillin for H. pylori eradication related to these important host and bacterial factors in Thailand. Materials and Methods: This prospective randomized study was performed during March 2015 to January 2016. H. pylori infected gastritis patients were randomized to receive 10-day sequential therapy and 14-day standard triple therapy. CYP2C19 genotyping, IL1 polymorphism (IL-1B and IL-1RN genotypes) and antibiotic susceptibility tests were performed in all patients. 13C-UBT was conducted to confirm H. pylori eradication at least 4 weeks after treatment. Results: A total of 100 patients (33 males and 67 females, mean age=51.1 years) were enrolled. Eradication rate by PP analysis was 97.9% (47/48) with the 10-day SQT regimen and 87.8% (43/49) with 14-day STT regimen (97.9% vs 87.8%; p-value=0.053). Antibiotic susceptibility testing demonstrated 45% resistance to metronidazole, 14.8% to clarithromycin, and 24.1% to levofloxacin. CYP2C19 genotyping revealed 44.9% RM, 49% IM and 6.1% PM. IL-1B and IL-1RN genotypes were demonstrated as 21.4% for CC, 48.1% for TC, 36.8% for TT, 72.7% for 1/1, and 21.2% for 1/2 genotypes, respectively. The 10-day SQT regimen provided 100% eradication in patients with clarithromycin or dual clarithromycin and levofloxacin H. pylori resistant strains. Moreover, the 10-day SQT regimen resulted in a 100% eradication rate in all patients with CYP2C19 genotype RM and almost type of IL-1B (TC and TT) and IL1-RN genotypes ( 1/2 and other). Conclusions: Treatment with 10-day sequential therapy is highly effective for H. pylori eradication regardless of the effects of clarithromycin resistance, dual clarithromycin and levofloxacin resistance, CYP2C19 genotype, IL-1B and IL1-RN genetic polymorphisms and can be used as effective first line therapy in Thailand.

• Journal of Aquaculture
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• v.10 no.3
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• pp.335-346
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• 1997

Denitrification is one of the important processes of removing nitrate from in recirculating aquaculture systems. And this process is affected by many factors such as external organic carbon sources, hydraulic retention time (HRT), COD/NO3--N (C:N) ratio, etc. However, not many studies were done for the optimum conditions of denitrification in the recirculation system for aquaculture. Therefore, this study was conducted to find out the optimum removal condition of NO3--N using submerged denitrification biofilter. The combinations of two external organic carbon sources (glucose and methanol), two HRT (4 and 8-hour) and four differnent C : N ratios (3, 4, 5, 6) were tested. The removal efficiencies of NO3--N and total inorganic nitrogen (TIM) at 8-hour HRT were better than those at 4-hour's (P<0.05). The maximum removal efficiency of NO3--N by methanol (97.8%) was achieved at HRT and C : N ratio were 8-hour and 4.0 respectively. The efficiencies of methanol for the removal of NO3--N and TIN were always better than those of glucose (P<0.05). The maximum removal efficiencies of total inorgainc nitrogen (TIN) were gained at C : N ration of 5.0. The maximum removel efficiencies of TIN using methanol and glucose were 96.9% and 71.5% respectively. Anaerobic condition which is necessary for denitrification process was not made until the 8-hour HRT and higher C : N ratio (5.0). Removal of NO3--N at 4-hour HRT and C : N ration lower than 5.0 were inhibited by oxygen and/or low quantity of external organic carbon. Removal efficiencies of NO3--N were also inhibited by high C : N (6.0) ratio when HRT was 8-hour.

• Korean Journal of Food Science and Technology
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• v.34 no.6
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• pp.1067-1072
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• 2002

Peroxidase was used as a standard enzyme to determine optimum blanching conditions of Flammulina velutipes and Lyophyllum ulmarium. Crude peroxidase extracted from raw mushrooms had maximum activity at $10{\sim}15^{\circ}C$ and pH 5.5 (50 mM, potassium phosphate buffer) using substrates of $H_2O_2$ and p-Phenylendiamine. Thermal inactivation of the crude peroxidase followed the first-order kinetics. The activation energy and z value of the crude peroxidase for F. velutipes were 59.58 kcal/mol and $9.0^{\circ}C$, whereas were 43.05 kcal/mol and $12.4^{\circ}C$ for L. ulmarium, respectively. On the basis of thermal kinetics parameters obtained, the optimum blanching conditions for F. velutipes and L. ulmarium were 1 min at $70^{\circ}C$ and 5 min at $80^{\circ}C$, respectively. Activation energies and z values of peroxidases extracted from heat-treated mushrooms were 7.97 and 6.55 kcal/mol, and $59.8^{\circ}C\;and\;74.1^{\circ}C$ for F. velutipes and L. ulmarium, respectively.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1743-1750
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• 2007

Two Gram-negative, nonmotile, coccobacilli, SW-$3^T$ and SW-$100^T$, were isolated from sea water of the Yellow Sea in Korea. Strains SW-$3^T$ and SW-$100^T$ contained ubiquinone-9 (Q-9) as the predominant respiratory lipoquinone and $C_{18:1}\;{\omega}9c$ and $C_{16:0}$ as the major fatty acids. The DNA G+C contents of strains SW-$3^T$ and SW- $100^T$ were 44.1 mol% and 41.9 mol%, respectively. A neighbor-joining tree based on l6S rRNA gene sequences showed that the two isolates fell within the evolutionary radiation enclosed by the genus Acinetobacter. Strains SW-$3^T$ and SW-$100^T$ exhibited a l6S rRNA gene similarity value of 95.7% and a mean DNA-DNA relatedness level of 9.2%. Strain SW-$3^T$ exhibited l6S rRNA gene sequence similarity levels of 93.5-96.9% to the validly described Acinetobacter species and fifteen Acinetobacter genomic species. Strain SW-$100^T$ exhibited l6S rRNA gene sequence similarity levels of less than 97.0% to the other Acinetobacter species except Acinetobacter towneri DSM $14962^T$ (98.0% similarity). Strains SW-$3^T$ and SW-$100^T$ exhibited mean levels of DNA-DNA relatedness of 7.3-l6.7% to the type strains of some phylogenetically related Acinetobacter species. On the basis of phenotypic, phylogenetic, and genetic data, strains SW-$3^T$ and SW-$100^T$ were classified in the genus Acinetobacter as two distinct novel species, for which the names Acinetobacter marinus sp. novo (type strain SW-$3^T$=KCTC $12259^T$=DSM $16312^T$) and Acinetobacter seohaensis sp. novo (type strain SW-$100^T$=KCTC $12260^T$=DSM $16313^T$) are proposed, respectively.

• Korean Journal of Environmental Agriculture
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• v.19 no.4
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• pp.300-308
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• 2000

The degradation of herbicide $^{14}C-bifenox$ was studied in soils under anaerobic conditions. $^{14}C-bifenox$ was treated in silty loam and sandy loam soils, respectively at a rate of 2.1 mg/kg, and the soil was incubated under anaerobic conditions at $25^{\circ}C$ for 180 days. The mineralization, solvent extractable and non-extractable residues, degradation products of bifenox were investigated during the experiments. The relative amounts of $^{14}CO_2$ were 1.97 and 0.9% of applied $^{14}C$ in silty loam and sandy loam soils, respectively. The non-extractable residues of sandy loam soil increased dramatically up to 79.12% of applied $^{14}C$, and were higher than those of silt loam soil, suggesting physico-chemical properties and especially organic matter contributed to the difference of $^{14}C$ between two soils. The non-extractable residues were formed mainly humin fraction and increased with time. The major metabolites were nitrofen, 5-(2,4-dichlorophenoxy)-2-Nitrobenzoate, 2,4-dichlorophenoxy aniline and methyl 5-(2,4-dichlorophenoxy) anthranilate by GC/MS analysis. From the results of volatilization, mineralization and degradation of bifenox, bifenox was stable chemically and biologically in soil.

• Korean journal of applied entomology
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• v.40 no.2
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• pp.131-136
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• 2001

Cabbage butterfly, Pieris rapae was reared in a room to establish a year-round rearing system. The eggs oviposited by the parent fed on host plant showed 89.2% of hatchability and hatched in 3.9 days after oviposition. The larval period was 18.1 days under high temperature, long day condition ($25^{\circ}C$, 16L : 8D), showing 97.8% pupal ratio and emergence rate. However, under low temperature, short day condition ($21^{\circ}C$, 10L : 14D) the larval period extended to 23.6 days and the pupal ratio was 70%. All of those pupae went into diapause. The oviposition preference experiment on different hosts (Chinese cabbage, cabbage, tulip and kale) showed that hot-water extract was preferred over methanol extracts or squeezed raw juices. The host preference showed that Chinese cabbage was less preferred than the other three. The artificial ovipositing kit was constructed for the oviposition in a room. The 48-hours old eggs could be stored for 7 days at$ 5^{\circ}C$ and showed 70% of hatchability. Non-diapausing pupae could be stored for 30 days at 5 to $15^{\circ}C$, showing 85% of emergence rate. However, the pupae stored at $5^{\circ}C$ showed longer storage period and higher emergence rate. The systematic successive rearing method of cabbage butterfly in a room was completed, based on the above experiments.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.1
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• pp.49-56
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• 2009

The objective was to determine the effects of supplementation of protected conjugated linoleic acid (CLA), CLA-20 comprising 10% each of cis-9, trans-11 and trans-10, cis-12, on milk production and fatty acid profiles in plasma and milk in lactating dairy cows. Five mid-lactation, multiparous crossbred Holstein Friesian cows with average 402${\pm}$20 kg BW were used in a 5${\times}$5 Latin square design for 21-d periods. Cows were given a total mixed ration (TMR) and supplemented with CLA-20 at 0, 20, 40, 80 and 160 g/d. The results showed that dry matter intake depression occurred in cows supplemented with CLA-20 at 160 g/d. Milk production slightly increased when CLA-20 supplementation was at 20, 40 and 80 g/d. However, 3.5% fat-corrected milk (FCM) was not affected by CLA-20 supplementation. Increased levels of CLA-20 supplementation resulted in a significantly decreased percentage of milk fat. Plasma concentrations of fatty acid were not altered by the amounts of CLA-20 supplementation except for the concentration of trans-10, cis-12 CLA. For all dietary treatments, percentages of fatty acids (C4:0, C6:0, C8:0, C13:0, C14:0 C14:1 C15:0 C15:1 C16:0, C16:1, C18:1n9t, C18:2n6t, C18:2n6c, C20:0, C18:3n6, C18:3n3, C20:1 and C20:3n6) in milk fat were similar. Concentrations of C10:0, C11:0, C12:0 and C18:1n9c were decreased cubically and C18:0 was elevated linearly (p<0.01) according to the increased amounts of CLA-20 supplemented. The linear increase was observed for cis-9, trans-11 CLA (0.62, 1.17, 1.94, 1.87 and 1.82% of total fatty acid), trans-10, cis-12 CLA (0.01, 0.63, 0.67, 0.93 and 0.95% of total fatty acid) and total CLA (0.80, 2.25, 3.16, 3.97 and 3.94% of total fatty acid) in milk fat from 0 to 160 g/d of CLA-20 supplement. In conclusion, concentration of cis-9, trans-11 CLA in milk fat was concomitantly elevated at an increasing rate with the increased amounts of CLA-20. Based on the results in this study, supplementation of CLA-20 at 80 g/d optimally enhanced total CLA in milk fat.

• Korean Journal of Veterinary Service
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• v.21 no.2
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• pp.141-148
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• 1998

Eighteen strains of Clostpidium perfringens were isolated from the piglets with hemorrhagic enteritis. The characteristics of the outbreaks, clinical signs and lesions were examined. The biochemical properties, type of toxins and susceptibility to antimicrobial agents against the isolates were investigated. 1. The incidence of diarrhea was appeared in 97(22.4%) of 432 piglets examined. 2. The isolation rate of Cl perfingens from the 97 diarrheal faeces were 18.5%(18 strains) 3. The population of Cl perfingens in feces were ranged $10^{8-9}$cfu/g in 5(32.5%) and $10^{3-7}$cfu/g in 13(67.4%) of 18 samples. 4. The toxin type of the 18 isolates investigated by mouse inoculation test was all type C strains of Cl perfringens. 5. As a results of antimicrobial susceptibility test, 18 isolates were higly susceptible to cephalothin, tetracycline and penicillin.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.1
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• pp.83-88
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• 2014

This research was carried out to determine the characteristics of germination in relation to temperature, and seedling emergence and growth affected by soil moisture in forage millet. The seeds of forage millet could germinate at more than $15^{\circ}C$ at the rate of 0, 90.5, 97.3 and 96.8% respectively 10 days after seeding at 10, 15, 20 and $25^{\circ}C$. The result showed that the temperature needs to be $15^{\circ}C$ or more for the seeds to germinate. The effects of seeding depth on the growth characteristics and emergence of millet seed were investigated in waters and direct seeding methods by thoroughly mixing the seed and soil. In water treatment, the emergence rates were 3.7% at $15^{\circ}C$, 7.6% at $20^{\circ}C$, 6.3% at $25^{\circ}C$, while direct seeding treatment showed the emergence rates of 65.6, 75 and 71% at 15, 20 and $25^{\circ}C$, respectively. At 15, 20, and $25^{\circ}C$, seeds in water seeding could germinate at 0.5, 1.8, 1.5 cm of soil depth, while in direct seeding, they could germinate at the depth of 9.5, 10.0, 9.9 cm. When the initial growth characteristics of the seeds were investigated, there was positive correlation between seeding depth and mesocotyl length in both water and direct seed methods. Growth and development in terms of leaf age, root length, coleoptiles and mesocotyl length, and plant height were found maximum at 0.5 cm soil depth for water seeding, and 3-5 cm soil depth for direct seeding. Results showed that there were close relationship among temperature, soil depth and moisture acquiring methods, and the combination of these factors greatly affected the initial growth characteristics and development of millet seeds. It can be concluded that, to get good seedling stand and germination of millet seed, millet should grown in field condition at the depth of 0.5 cm or less for water seeding, and 3-5 cm for direct seeding method.

• Proceedings of the IEEK Conference
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• 1998.10a
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• pp.727-730
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• 1998

In this paper, we propose an implementation of an efficient parallel Radon transform on TMS320C80-based system. For an N$\times$N SAR image, we can obtain O(NM/p) of the conventional parallel Radon transform, by representing the projection patterns in Radon space variables instead of the image space variables, and pipelining the algorithm, where p is the number of processors and M is the number of projection angles. Also, we can reduce the time for the dynamic load distribution among the nodes and the communication overheads of accessing the global memories, by pipelining the memory and processing operations by using tripple buffer structure. Experimental results show an efficient parallel Radon transform of speedup Sp=3.9 and efficiency E=97.5% for 256$\times$256 image, when implemented on TMS320C80 composed of four parallel slave processors with three memory blocks.