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Anti-inflammation Activities of Cultured Products from Suspension Culture of Aloe vera Callus (Aloe vera Callus 현탁배양 생성물의 항염증 활성)

  • Kim, Myung Uk;Cho, Young Je;Lee, Shin Young
    • Journal of Applied Biological Chemistry
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    • v.56 no.3
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    • pp.157-163
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    • 2013
  • Cultured products (callus and exopolysaccharide) were obtained from suspension culture of Aloe vera callus, and the extracts of callus were further prepared with cold water or 60% ethanol solution. The ethanol extract of callus (AC) and exopolysaccharide (ACP) of 10 mg/mL exhibited the relatively higher suppression activity of 43.2-52.1% against hyaluronidase activity. Thus, their anti-inflammatory effects were further investigated using animal cell (Raw 264.7) in vitro. Though AC shows a slight suppression effect of cell survival rate (97%) using MTT assay in the presence of $400{\mu}g/mL$ AC- dimethyl sulfoxide (DMSO), cell growth promotion was observed in the other samples of lower levels. It indicates that the ethanol extract of Aloe callus rarely affect cell survival rate in the ranges ($200-400{\mu}g/mL$) used in the study. Using Griess reagent, the suppression of NO production by the aloe callus extract was analyzed by measuring the amount of the nitrite produced in Raw 264.7 culture activated by lipopolysaccharide (LPS). As a result, supplementation of AC-distilled water (DW) and AC-DMSO produced higher levels of NO than the positive control LPS. However, the NO suppression effect by ACP-DW was so intense that lower amount ($80-100{\mu}g/mL$) suppressed NO production to the level of the control. The effect was attributed to the expression of the iNOS. Then, Raw 264.7 cells were stimulated with the LPS and expression of COX-2 protein level was analyzed depending on the Aloe suspension culture product treatment. The results showed that the ACP-DW supplemented medium did not express COX-2 by itself, and LPS stimulated COX-2 expression was slightly decreased. On the other hand, realtime-PCR analysis of the expression of inflammatory cytokine showed that IL-$1{\beta}$ and TNF-${\alpha}$ expression was highly suppressed in the ACP- distilled water supplemented medium.

Effects of Feeding Dehulled Soybean Meal on Growth Performance, Carcass Characteristics and Meat Quality Parameters in Berkshire Pigs (탈피대두박 급여가 흑돼지의 생산성, 도체 및 육질 특성에 미치는 영향)

  • Song, Y.M.;Kim, H.Y.;Sin, I.S.;Lee, H.S.;Lee, S.D.;Jung, H.J.;Park, J.C.;Moon, H.K.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.69-78
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    • 2008
  • A total of 360 Berkshire(hog) weighing 25kg were fed experimental diets containing either dehulled soybean meal(DSBM) or non-dehulled soybean meal(NDSBM) as a major protein source until they reach 80kg. The pigs were then fed the finisher diets either high(3,265 kcal/kg) or low(2,940 kcal/kg) in ME to evaluate the effects of dietary energy level for finisher phase on the growth performances, carcass and meat quality parameter. In growth performance, daily weight gain(kg/day) was higher(P<0.05) in T1 than in other groups. Feed conversion(feed/gain) was lower(P<0.05) in other groups. In carcass parameter, carcass percentage was higher(P<0.05) in T2 than in T1 and T4. Backfat thickness was lower(P<0.05) in T2 than in T1 and T3. The ratio of up B grade in carcass grade tender to be higher in T2 than in other groups. In meat quality, chemical composition was similar between groups. WHC was slighter higher in T1 than in other groups. Shear force was tender to be higher in T1 than in other groups. Hunter L and a in meat color were tender to be higher in T2 than in other groups. Hunter L in backfat color was thender to be higher in T1 than in other groups. The results indicate that growth performance and carcass parameter of Berkshire were improved by the dehulled soybean meal as comparison with non-dehulled soybean meal during growing and finishing periods.

Attitudes of Safety for the Food Developed by Gene-Recombination in Korea -Focus on the Specialist Group Related to Food- (유전자재조합 식품의 안전성에 대한 기본인식 조사 - 식품관련 전문가 그룹을 중심으로 -)

  • 김영찬;박경진;이홍석;김동연
    • Journal of Food Hygiene and Safety
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    • v.14 no.4
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    • pp.397-407
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    • 1999
  • A survey was conducted to investigate the attitudes of the food specialist to the food developed by gene recombination. The mail survey was distributed to 1,400 food specialists and received 464, a response rate of 33.1%. Respondents were asked about knowledge, concerns of potential hazards, purchasing and labeling of the gene recombination foods. Most respondents (98.7%) have some knowledge on the gene recombination foods. 91.3% of respondents recognized necessity of gene recombination technology. However, they also point out its potential hazards (80.9%). The groups with less knowledge showed their increased worry on the hazard in comparison with ones of having more knowledge(p<0.01). The result indicated that there was negative relationship between knowledge and worry on the gene recombination foods. The groups with more knowledge showed their increased purchasing on gene recombination foods in comparison with ones of having less knowledge(p<0.01). The result indicated that there was positive relation-ship between their purchase intent for gene recombination foods and knowledge. 68.4% of respondents showed their interest on purchasing the gene recombination foods. In this group, most of them (44.9%) has on condition that low cost (27.0%). In addition, they also have not use the foods for their children (17.9%) if they buy it. Most respondents (85.3%) want labeling on the gene recombination foods.

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Mercury Contents of Paddy Soil in Korea and its Uptake to Rice Plant (우리나라 논 토양 중 수은함량과 벼 흡수이행)

  • Park, Sang-Won;Yang, Ju-Seok;Kim, Jin-Kyoung;Park, Byung-Jun;Kim, Won-Il;Choi, Ju-Hyeon;Kwon, Oh-Kyung;Ryu, Gab-Hee
    • Journal of Food Hygiene and Safety
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    • v.23 no.1
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    • pp.6-14
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    • 2008
  • Objective of this study was to investigate the residual levels of mercury (Hg) in soil for "Top-rice" area and its uptake into rice plant for making sure food safety as compared to "Top-rice" & common rice produced from 2005 to 2006. Hg was analyzed with the direct mercury analyzer (DMA 80, Milestone, Italy), which implements the US/EPA method 7473. The average concentration of Hg in paddy soil was 0.031 mg/kg, which was below at 1/25-1/65 fold of the threshold levels (concern level 4 mg/kg, action level 10 mg/kg) for soil contamination designated by "The Soil Environment Conservation Law" in Korea. The maximum residue level (MRLs) for Hg residue in the polished rice is not designated in Korea. Therefore, Hg contents in the polished rice of "Top-rice" brand and common rice were compared to other country's criteria. Hg contents in the polished rice of "Top-rice" brand was 0.0018 mg/kg, which was lower at 1/10-1/30 fold than the MRLs, 0.02 mg/kg of China criteria and 0.05 mg/kg of Taiwan criteria, respectively. Hg were 0.02788, 0.00896, 0.00182, 0.00189, 0.00166, 0.00452 and 0.00145 mg/kg in soil, rice straw, unhulled rice, rice hulls, brown rice, rice bran, and polished rice produced in 2006 "Top-rice" area, respectively. For the ratio of Hg as compared to Hg contents in soil, there were 0.321 of rice straw ${\gg}$ 0.162 of rice bran ${\gg}$ 0.068 of rice hulls > 0.065 of unhulled rice > 0.060 of brown rice> 0.052 of polished rice. And, the slope of Hg uptakes was steeped as following order; rice straw ${\gg}$ rice bran ${\gg}$ rice hulls > unhulled rice > brown rice > polished rice. It means that the more slope steeped was the more uptakes. For the distribution of Hg uptaken, there was 83.8% into rice straw, and 16.2% into unhulled rice, 2.8% into rice hulls, 12.4% into brown rice, 3.5% into rice bran and 9.7% into polished rice. Consequently, it was appeared that the Hg contamination in the polished rice should not be worried in Korea.

Studies on the Manufacture and Production of Enzyme Utilizing for Food Industry -Development of the powerful microbial pectic enzyme utilizing for the clarification of fruit juice- (식품공업(食品工業)에 이용(利用)되는 효소(酵素)의 생산(生産)과 제품화(製品化)에 관(關)한 연구(硏究) -과즙(果汁)의 청징(淸澄)에 사용(使用)하는 강력(强力)한 미생물(微生物)펙틴분해효소(分解酵素)의 개발(開發)-)

  • Chung, Man-Jae
    • Korean Journal of Food Science and Technology
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    • v.10 no.2
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    • pp.237-244
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    • 1978
  • Among the strains isolated form the various sources, the strain AC-12 producing a powerful pectinase was selected by the extensive screening test. The selected strain was indentified and its toxicity investigated. The conditions of the pectinase production, the characteristics of the purified enzyme and the clarification effect on the apple juice were studied. 1. The selected strain AC-12 was identified by the classification method of paper and fennel and named as Aspergillus sp. AC-12. 2. As a result of the breeding test of the white mouse, no toxicity was found from this enzyme. 3. The yield of pectinase in the medium of defatted rice bran was much better than that in the medium of wheat bran. 4. The optimum conditions for the culture of the strain in the medium of defatted rice bran were that the cultural time was 72hrs, the amount of water to be added about 80%, temperature $30{\sim}35^{\circ}C$ and pH $3.0{\sim}5.0$. 5. The yield of pectinase was slightly increased by the addition of pectin to the medium of defatted rice bran and by the addition of pectin, $NaNO_3$ and $K_2HPO_4$ to the medium of wheat bran, respectively. 6. The optimum conditions for the enzyme activity were pH $3.0{\sim}4.0$ and temperature $40{\sim}50^{\circ}C$. The enzyme was stable below $40^{\circ}C$ and pH $2.0{\sim}8.0$, respectively. But above $50^{\circ}C,$ this enzyme was abruptly inactivated. The activity was slightly increased by the addition of $MnSO_4\;and\;CuSO_4.$ 7. It was regarded that the opimum temperature for the clarification of the apple juice was $40{\sim}50^{\circ}C$, the optimum pH 3.0 and the optimun concentration of the enzyme 0.1%, and the apple juice was almost clarified by the reaction at $45^{\circ}C$ for 60 minutes.

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Spatial Ability, Its Relationship to Mathematics Achievement, and Strategic Choices for Spatial Tasks Among Engineering Freshmen, and Gender Differences (공과대학 신입생들의 공간 시각화 능력의 수학 성취도와의 관계와 문제해결 전략 및 성별 차이에 관한 연구)

  • Kim, Yon Mi
    • Korean Journal of Cognitive Science
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    • v.28 no.3
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    • pp.149-171
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    • 2017
  • In this research, based on the fact that spatial ability is important for the achievement in the STEM fields, and technological innovation, Purdue Spatial Visualization Test-Rotation has been used to investigate engineering freshmen's spatial ability and gender differences. Students who have taken advanced mathematics courses in high school(those who have taken type B math test in Korean SAT test) and students with general math courses(those who have taken type A in Korean SAT-Math test) are included in this study to find out the relationship between mathematics achievement and spatial ability. Finding out the strategies taken by students was another aim of this study. This strategic differences between high achievers and lower achievers, male and female students were analyzed from students' self report. Spatial ability test score was highest in the SAT-Math type B male students, decreased in the order of type A male students, type B female students, and lastly type A female students. There was no substantial difference between second and third groups. In each group, male students' average score was 8~10% higher than female students, which affirms 2015's results. The correlation between spatial ability and mathematics achievement was negligible in each group, but male students' math score and spatial ability score were higher than that of female students. This can be interpreted that there is some correlation between these two. Strategic choices can vary in the continuous spectrum with analytic method and holistic method at both ends. From students' self report, using Mann-Witney test, it turned out that there exists strategic differences between male and female students. Male students have a tendency to use holistic strategy more often than female students. I also found that the strategy choice did not vary greatly among all score groups. For the perfect score groups, both female and male students used holistic strategy most frequently. For low achieving groups, there is an evidence that these students overuse one method compared to average or high achieving groups, which turned out to be less effective. Based on these, I suggest that low achieving students need to have more chances to adopt efficient strategies and to practice challenging problems to improve their spatial abilities.

Studies on Efficient Plowing Methods and the Shapes of Field for 4 Wheel Tractor (사수트렉터를 위한 효율적인 정기방법과 포장형상에 관한 연구)

  • 원장우
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.12 no.3
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    • pp.2019-2028
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    • 1970
  • 1. Experiments were made for the efficient plowing method by the 4 wheel tractor, the results are as follow; 1) In case of plowing of inner side of the field, the efficient turning method to be the smallest turning time is the $\Omega$-shaped turning method in the turning distance less than 2r (r is the minimum turning radius of the tractor), and also, it is the U-shaped turning method in the turning distance larger than 2r. 2) 2.5r is most efficient in the unit turning section 'w' on plowing of the inner side of the field. 3) In case of plowing of outer side of the field, intermitted plowing method is efficient in case of W>-0.0345 L + 35.84, and also, semi-followed plowing method is efficient in case of W<-0.0345 L + 35.84. 4) The smaller the width of outer side of outer side of the field 'I' is, the higher is the plowing efficiency, and it is estimated that the minimum value 2r is suitable to 'I' in plowing of inner side and outer side of the field. 2. Study on the correlation between the unit field and plowing efficiencies obtained the following results; 1) plowing efficienies increase generally according as length-width ratio L/W and area A increase. 2) Percent of increase of plowing efficiencies decreases generally according as length-width ratio and area enlarge. 3) The limit that change of T is large owing to L/W is 6 for 20 a, 5 for 30 a, 4 for 50 a, 3 for 80 a, less than 2.5 for 100 a, generally, in L/W-T curve. 4) Rate of change of T-A curve is similar to rate of change of $T=A-\frac{2}{3}$ curve in spite of influence of L/W. 5) In case that length-width ratio is more than 3, effects of increase of 10 a area influenced upon plowing efficiencies are as much as effects of about 5 increase of length width ratio without correlation of size of the field. 6) In case that length-width ratio is 2 to 3, effects of increase of 10 a area influenced upon plowing efficiencies are as much as effects of about 4 to 2 increase of length-width ratio without correlation of size of the field, and the effects decrease according as not only length-width ratio decreases but also area increases, generally.

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Hydrochemistry of Groundwater at Natural Mineral Water Plants in the Okcheon Metamorphic Belt (옥천계변성암 지역의 먹는샘물 지하수의 수리지구화학적 특성)

  • 추창오;성익환;조병욱;이병대;김통권
    • Journal of Korea Soil Environment Society
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    • v.3 no.3
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    • pp.93-107
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    • 1998
  • Because of its stable quantity and quality, groundwater has long been a reliable source of drinking water for domestic users. Rapid economic growth and rising standards of living have in recent years put severe demands on drinking water supplies in Korea. Groundwaters that are currently being used for natural mineral water were hydrochemically evaluated and investigated in order to maintain their quality to satisfy strict health standards. There exist 15 natural mineral water plants in the Okcheon metamorphic belt. Characteristics of groundwaters are different from those of other areas in that electrical conductivity, hardness, contents of Ca, Mg and $HCO_3$are relatively high. The content of major cations is in the order of Ca>Mg, Na>K, whereas that of major anions shows the order of $HCO_3$>$SO_4$>Cl>F. The fact that the Ca-Mg-HCO$_3$type is mostly predominant among water types reflects that dissolution of carbonates that are abundantly present in the metamorphic rocks plays an important part in groundwater chemistry. Representative correlation coefficients between chemical species show Mg-$HCO_3$(0.92), Ca-$HCO_3$(0.88), Ca-Mg(0.80), Ca-Cl(0.78), Mg-$SO_4$(0.78), Ca-$SO_4$(0.71), possibly due to the effect by dissolution of carbonates, gypsum or anhydrite. Determinative coefficients between some chemical species represent a good relationship, especially for EC-(K+Na+Ca), Ca-$HCO_3$, Ca-Mg, indiacting that they are similar in chemical behaviors. According to saturation index, most chemical species are undersaturated with respect to major minerals, except for some silica phases. Groundwater is slightly undersaturated with respect to calcite and dolomite, whereas it is still greatly undersaturated with respect to gypsum, anhydrite and fluorite, Based on the Phase equilibrium in the systems $NA_2$O-$Al_2$$O_3$-$SiO_2$-$H_2$O and $K_2$O-$Al_2$$O_3$-$SiO_2$-$H_2$O, it is clear that groundwater is in equilibrium with kaolinite, evolved from the stability area of gibbsite during water-rock interaction. It is expected that chemical evolution of groundwater continue to proceed with increasing pH by reaction of feldspars, with calcite much less reactive.

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Artificial Cultivation of Tricholoma giganteum Collected in Korea (I) - Morphological Charateristics of Fruitbody and Environmental Condition in Habitat of T. giganteum - (한국산 왕송이버섯의 인공재배 (I) - 자실체 형태적 특징과 발생지 환경조사 -)

  • Kim, Han-Kyoung;Kim, Yang-Sup;Seok, Soon-Ja;Kim, Gwang-Po;Cha, Dong-Yeul
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.182-186
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    • 1998
  • The result of study on the morphological feature of fruitbody, soil characteristics and enviromental condition of habitat of Tricholoma giganteum in Korea was as follows: The Pileus was $5.5{\sim}28.0\;cm$ in diameter and $1.5{\sim}3.7\;cm$ in thickness, indicating of yellowish white, beige, or ivory color. The lamellae were $18{\sim}20\;mm$ in diameter; the stipe was $9.0{\sim}35.7\;cm$ long and $1.0{\sim}3.0\;cm$ wide. The spores were $3.5{\sim}4.8{\times}5.7{\sim}7.4\;{\mu}m$ in size, ellipsoid, hyaline and the spore deposit white. The basidia were $6.1{\sim}7.0{\times}32.2{\sim}39.2\;{\mu}m$ in size. Cheiloystidia were $3.5{\sim}4.4{\times}30.5{\sim}33.1\;{\mu}m$ in size. Pileipellis were $3.3{\sim}4.4{\times}33.0{\sim}55.0\;{\mu}m$ wide and had clamp connection. Stipitipellis were $2.2{\sim}3.3{\times}88.0{\sim}93.1\;{\mu}m$ in size. Texture of soil in habitate of T. giganteum was silty loam, and organic materials content and available phosphate content in this soil were high as comparision to general soil in Korea. In the time of the mushrooms sprout, the room temperature indicated $25{\sim}27^{\circ}C$, their humidity showed $80{\sim}83%$, and the brightnesses were 328 Lux.

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Effect of Orthodontics Patients' Orthodontics Characteristics on the Living Quality Related to Oral Health (OHIP) (교정환자의 교정치료 특징이 구강건강관련 삶의 질(OHIP)에 미치는 영향)

  • Yoon, Sung-Uk;Oh, Na-Rae;Jeong, Mi-Ae
    • The Journal of the Korea Contents Association
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    • v.14 no.3
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    • pp.250-258
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    • 2014
  • The purpose of this study was to promote effective health management of orthodontics patients by determining the effect that the factors related to orthodontics would have on the Oral Health Impact Profile (OHIP), and the subjects of this study was the local residents of Gangwon-do Province. The mean score of OHIP for the subjects was 2.97, while the mean score of physical factor was 3.01. The mean score of psychological factor was found to be 2.93. Thus, the orthodontics was found to have more influence on psychological factor. The item that had the greatest influence among the 10 items of Oral Health Impact Profile was found to be the question, "Have you ever felt shy due to your dental shape?" which had the mean score of 2.66. The characteristics that had significant effect on OHIP among general characteristics were found to be the age, religion, occupation, income, smoking or non-smoking status(p<0.05). In terms of the age, the age of 20 or less had the greatest effect with the mean score of 2.53. In relation to the occupation, the production/sales occupation had an influence with the mean score of 2.56 (p<0.05). Those with income exceeding KRW 4 million were found to have the mean score of 2.83 and 2.78. It turned out that the smokers had greater effect compared to the non-smokers in relation to the smoking/non-smoking status as the income was higher (p<0.05). The characteristics of orthodontics were manifested as the health perception in relation to Oral Health Impact Profile (OHIP) (p<0.05). Regarding the reason why they underwent the orthodontics, the 'uncomfortable feeling in chewing' was found to have the mean score of 2.69 and 2.67 (p<0.05). Regarding the orthodontics period, 1 to 2 years were found to have the mean score of 2.80 and 2.74, thus having an influence (p<0.05). In relation to the regret/non-regret over orthodontics, those who indicated that they regretted were found to have the mean score of 2.65 and 2.60 (p<0.05). Analysis of the relationship between Characteristics of orthodontic treatment and OHIP, orthodontic treatment reasons, oral health awareness, whether orthodontic treatment regret showed a statistically significant correlation. it is considered necessary to make constant efforts to help orthodontics patients, both physically and psychologically, in the course of treatment and improve their quality of lives.