• 농업과학연구
• /
• 제45권2호
• /
• pp.248-257
• /
• 2018

Overexpression of AtCYP78A7, a gene encoding a cytochrome P450 protein, has been reported to improve tolerance to drought stress in genetically modified (GM) rice (Oryza sativa L.). The aim of this study was to evaluate the potential allergenicity and acute oral toxicity of the AtCYP78A7 protein expressed in GM rice. Bioinformatics analysis of the amino acid sequence of AtCYP78A7 did not identify any similarities with any known allergens or toxins. It showed that no known allergen had more than a 35% amino acid sequence homology with the AtCYP78A7 protein over an 80 amino acid window or more than 8 consecutive identical amino acids. The gene encoding the AtCYP78A7 protein was cloned in the pGEX-4T-1 vector and expressed in E. coli. Then, the AtCYP78A7 protein was purified and analyzed for acute oral toxicity. The AtCYP78A7 protein was fed at a dose of 2,000 mg/kg body weight in mice, and the changes in mortalities, clinical findings, and body weight were monitored for 14 days after the dosing. Necropsy was carried out on day 14. The protein did not cause any adverse effects when it was orally administered to mice at 2000 mg/kg body weight. These results indicate that the AtCYP78A7 protein expressed in GM rice would not be a potential allergen or toxin.

• Journal of Microbiology and Biotechnology
• /
• 제29권1호
• /
• pp.114-126
• /
• 2019

This paper introduces three ways to determine host-guest complexation of cucurbit[7]uril (CB[7]) with homocysteine (Hcy). After preincubating Hcy and cysteine (Cys) with CB[7], Ellman's reagent (DTNB) was used to detect Hcy and Cys. Only Cys reacted with DTNB and Hcy gave a retarded color change. This suggests that the -SH group of Hcy is buried inside CB[7]. Human cystathionine ${\gamma}-lyase$ (hCGL) decreased the level of Hcy degradation after preincubating Hcy and CB[7]. These results suggest that the amount of free Hcy available was decreased by the formation of a Hcy-CB[7] complex. The immunological signal of anti-Hcy monoclonal antibody was decreased significantly by preincubating CB[7] with Hcy. The ELISA results also show that ethanethiol group ($-CH_2CH_2SH$) of Hcy, which is an epitope of anti-Hcy monoclonal antibody, was blocked by the cavity in CB[7]. Overall, CB[7] can act as a host by binding selectively with Hcy, but not Cys. The calculated half-complexation formation concentration of CB[7] was 58.2 nmol using Ellman's protocol, 97.9 nmol using hCGL assay and 87.7 nmol using monoclonal antibody. The differing binding abilities of Hcy and Cys towards the CB[7] host may offer a simple and useful method for determining the Hcy concentration in plasma or serum.

• 분석과학
• /
• 제17권5호
• /
• pp.401-409
• /
• 2004

7-keto-DHEA-acetate는 DHEA의 대사체인 7-keto-DHEA로 변하고 체내에서 대사되는 과정이 서로 유사하였으며 복용 후 검출된 대사체 M3, M4 및 M5에 대해 살펴보았다. 대사체 M3과 M4는 분자량이 304이며 물분자가 제거될 수 있는 히드록시기나 케톤기를 세 개 이상 가진 구조로 예상되었으며, M3의 경우 3번과 7번 탄소위치에 히드록시기가 있고 17번 탄소위치에 케톤기를 가지는 7-OH-DHEA이라는 것을 알 수 있었다. 대사체 M4는 3번과 17번 탄소위치에 히드록시기가 있고, 7번 탄소위치에 케톤기가 있는 구조인 7-oxo-diol 이었다. 대사체 M5는 분자량이 320이며 M3 및 M4와는 달리 물분자가 제거될 수 있는 히드록시기나 케톤기를 네 개 이상 가진 구조일 것이라고 예상되었으며, 3번과 7번 그리고 16번 탄소위치에 히드록시기가, 그리고 17번 탄소위치에 케톤기가 있을 것으로 예상되었다. 7-keto-DHEA-acetate 복용 후 최대 배설량을 보인 대사체는 7-OH-DHEA 이었으며, 복용 5시간 후 최대의 배설량을 보인 이후 급격히 감소하였으며 매우 빠른 속도로 체외로 배설되기도 하지만 복용 58시간 후의 뇨 시료에서도 검출되기 때문에 체내 잔류성이 높은 약물임을 알 수 있었다.

• 대한화장품학회지
• /
• 제46권2호
• /
• pp.147-157
• /
• 2020

서해안에 서식하는 해양생물인 전복은 항산화 및 항염증 효과가 있으며, 항생제 개발 및 화장품 원료 등 다양한 산업에 활용될 수 있는 잠재력을 가진 자원이다. 본 연구에서 우리는 서해안의 다양한 해양 생물 중에서 전복을 선택하였으며, 전복에서 분리한 항균펩타이드(AMP)를 이용하여 항균펩타이드 유도체인 Ab4-7를 규명하고 생리활성을 연구하였다. Ab4-7의 항염증 효능을 확인하기 위해 염증이 유도된 RAW 264.7에 Ab4-7을 처리한 결과, 염증성 사이토카인, TLR4, TNF-α, IL-1β, COX-2, iNOS 등을 억제하고 항산화와 관련된 유전인자인 HO-1의 mRNA 발현을 증가시켰다. Ab4-7의 강력한 항산화 및 항염증 효과를 바탕으로, 염증이 유도된 RAW 264.7 세포에서 Ab4-7이 extrocellular matrix metalloproteinase (ECM) 분해에 관여하여 다양한 염증성 피부질환을 유도하는 유전자인 matrix metalloproteinase (MMPs)를 조절하는 효과를 RT-PCR을 통해 확인하였다. Ab4-7은 강력한 항염증 및 항산화 효과를 가지고 있으며, 항염증 및 항산화 효과를 통한 MMPs 관련 유전인자 조절을 통해 다양한 염증성 피부질환 치료제의 기능성 재료로 활용될 수 있다고 사료된다.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2006년도 추계 종합학술대회 논문집
• /
• pp.52-57
• /
• 2006

본 연구에서는 디지털 코드리스폰(DCP)에 대한 현재 각국의 동향과 주파수 분배대역을 연구 분석하였다. 그 결과 국내 도입 가능한 후보주파수대역으로 1.7GHz와 2.4GHz대역을 제안하였다. 제안된 1.7GHz는 유럽의 DECT시스템이 도입될 것으로 예상되고 있어, 인접대역인 IMT-2000 후보주파수 대역간의 간섭분석이 필요하다. 본 논문에서는 1.7GHz대역과 IMT-2000 후보 주파수대역간의 간섭분석을 실시하여, 1.7GHz대역의 채널배치를 제안하였다.

• 한국멀티미디어학회논문지
• /
• 제10권1호
• /
• pp.1-12
• /
• 2007

모바일 환경의 제한된 자원 하에서 멀티미디어 데이터를 이용하기 위해서는 XML로 표현되는 MPEG-7 문서의 효율적인 관리 방법이 필요하다. 이 때 XML 문서 클러스터링 방법들을 이용할 수 있겠으나 보다 효율성을 높이기 위해 MPEG-7 문서의 특성을 반영한 새로운 클러스터링 방법이 요구된다. 새로운 클러스터링은 모바일 환경에서 멀티미디어 검색시 질의 처리 속도가 향상되며 다양한 응용에 적합한 문서 저장이 가능하다. 본 논문에서는 대용량의 멀티미디어 데이터를 MPEG-7 문서로 효율적으로 관리하기 위해 MPEG-7 문서의 엘리먼트들 사이에 나타나는 의미 관련성을 이용해 저장할 수 있는 새로운 클러스터링 방법을 제안하고, 이를 기존 방법들과 비교하였다.

• Archives of Pharmacal Research
• /
• 제23권4호
• /
• pp.288-301
• /
• 2000

A series of 2-alkyl, 2-aryl, and 2-piperazinyl benzimidazole-4,7-dione derivatives (7a-h) and 16m-o) were prepared, and their cytotoxicities were tested against three cancer cell lines (mouse lymphocytic leukemia cell line P388, and human gastric carcinoma cell lines SNU-1 and SNU-16). These compounds showed potent cytotoxicity against all of three cell lines tested, and especially SNU-16 was sensitive to them. 2-Aryl (7g,h) and 2-piperazinyl benzimidazole-4,7-dione derivative (I6 m) were more potent than mitomycin C against P388 and SNU-16. Among benzimidazole-4,7-dione derivatives with alkyl group at position 2, 7a had the most potent cytotoxicity against all of the cell lines tested.

• Journal of Microbiology and Biotechnology
• /
• 제20권2호
• /
• pp.265-270
• /
• 2010

Two major endoglucanase genes (cel7B and cel5A) were cloned from Penicillium decumbens 114-2 using the method of modified thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR). The result of Southern blotting suggested that P. decumbens has a single copy of the cel5A gene and a single copy of the cel7B gene in its chromosomal DNA. The expression levels of cel5A and cel7B were determined by means of real-time quantitative PCR, suggesting that the two genes were coordinately expressed, and repressed by glucose and induced by cellulose. Both endoglucanase genes were expressed in Saccharomyces cerevisiae and the recombinant proteins were purified. The recombinant Cel7B and Cel5A were both optimally active at $60^{\circ}C$ and pH 4.0. The recombinant Cel7B showed more than 8-fold, 30-fold, and 5-fold higher enzyme activities toward carboxymethyl cellulose, barley $\beta$-glucan, and PASC, respectively, in comparison with that of Cel5A. However, their activities toward pNPC and Avicel showed minor differences. The results suggested that Cel7B is a strict endoglucanase, whereas Cel5A showed processivity because of its relative higher ability to hydrolyze the crystal cellulose.

• Mycobiology
• /
• 제41권1호
• /
• pp.37-41
• /
• 2013

Laccases (EC 1.10.3.2) are copper-containing polyphenol oxidases found in white-rot fungi. Here, we report the cloning and analysis of the nucleotide sequence of a new laccase gene, fvlac7, based on the genomic sequence of Flammulina velutipes. A primer set was designed from the putative mRNA that was aligned to the genomic DNA of F. velutipes. A cDNA fragment approximately 1.6-kb long was then amplified by reverse transcriptase-PCR using total RNA, which was subsequently cloned and sequenced. The cDNA sequence of fvlac7 was then compared to that of the genomic DNA, and 16 introns were found in the genomic DNA sequence. The fvlac7 protein, which consists of 538 amino acids, showed only 42~51% identity with 12 different mushroom species containing two laccases of F. velutipes, suggesting the fvlac7 is a novel laccase gene. The first 25 amino acids of Fvlac7 correspond to a predicted signal sequence, four copper-binding sites, and four N-glycosylation sites. Fvlac7 cDNA was heterologously overexpressed in an Escherichia coli system with an approximate expected molecular weight of 60 kDa.

• 약학회지
• /
• 제38권1호
• /
• pp.86-90
• /
• 1994

7,8-Dioxo-A-norerythrinan (A) was synthesized from acid catalyzed cyclization of 6a-hydroxv-1-(2-phenylethyl)-octahydrocyclo-penta[b] pyrrole-3a-carboxylic acid ethyl ester (B) with concomitant deethoxycarbonylation. The intermediate (B) was a hydroxylated compound of N-acyliminium (C). The unstable pyrrolinium (C) was prepared from oxalylation of the enamine of phenethylamine and ethyl 2-oxocyclopentanecarboxylate.