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A study of the metabolites for 7-keto-DHEA-acetate in human urine (II)  

Kim, Yunje (Doping Control Center, Korea Institute of Science and Technology)
Lee, Jinhee (Doping Control Center, Korea Institute of Science and Technology)
Publication Information
Analytical Science and Technology / v.17, no.5, 2004 , pp. 401-409 More about this Journal
Abstract
The 7-keto-DHEA-acetate is converted to 7-keto-DHEA, a metabolite of DHEA, and similar to its metabolism. We studied the metabolite M3, M4, and M5 of 7-keto-DHEA-acetate. The estimated molecular weight of M3 and M4 was 304 which were supposed to have more 3 hydroxyl and/or ketone groups. We could know that M3 is the 7-OH-DHEA which has the hydroxyl groups on 3 and 7-carbon and a ketone group on 17-carbon. In case of M4, it is the 7-oxo-diol metabolite which has the hydroxyl groups on 3 and 17-carbon and a ketone group on 7-carbon. The M5 was supposed that the molecular weight is 320 and has the three hydroxyl groups on 3, 6, and 16 carbon and the ketone group on 17-carbon. After dosing, 7-OH-DHEA showed the maximum urine flow in human urine after 5 hr and decreased rapidly. But we could find it until 58 hr why is a higher remaining substance.
Keywords
7-keto-dehydroepiandrosterone-acetate; dosed human urine; metabolite M3; metabolite M4; metabolite M5; LC/ESI/MS; GC/MS;
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