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Inactivation of Escherichia coli O157:H7, Salmonella and Listeria monocytogenes by Organic Acid (유기산이 Escherichia coli O157:H7, Salmonella 및 Listeria monocytogenes의 증식에 미치는 영향)

  • Jang, Jae-Seon;Lee, Hye-Jeong;Oh, Bo-Young;Lee, Jea-Mann;Go, Jong-Myeong;Kim, Yong-Hee
    • Journal of Environmental Health Sciences
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    • v.33 no.5
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    • pp.403-407
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    • 2007
  • The inhibitory effect of the food processing agent on growth of Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes was performed with organic acid, and combination of citric acid, acetic acid, propionic acid and vanillic acid. The minimun inhibitory concentration(MIC) of propionic acid was 5,000 ppm in E. coli O157:H7, 2,500 ppm in Salmonella Enteritidis and Listeria monocytogenes. MIC of citric acid was 10,000 ppm in E. coli O157:H7 and Salmonella Enteritidis, 2,500 ppm in Listeria monocytogenes. MIC of acetic acid was 2,500ppm, while in vanillic acid was 5,000 ppm in Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes. MIC of combined organc acid in E. coli O157:H7 were 2,500ppm in PC, 1,250 ppm in PA, PV, CA, CV and AV. MIC of combined organc acid in Salmonella Enteritidis were 2,500 ppm in PC, PA, PV, CA, and CV, 1,250 ppm in AV. MIC of combined organc acid in Listeria monocytogenes were 1,250 ppm in all treatment group. MIC of combined treatment of three organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes were 1,250 ppm in PCA, PCV, PAV and CAV. The inhibitory effect of organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes could be confirmed from the result of this experiment. Therefore, it was expected that the food process would increase or maintain by using organic acid.

Analysis on the Tattoo Patterns used among Tattoo-related Internet Communities - Focusing on the Domestic and International Web Sites - (타투 관련 인터넷 동호회 사이트에 나타난 타투 문양 분석 - 국내.외 사이트를 중심으로 -)

  • Chung, Kyung-Hee;Lee, Mi-Sook
    • Journal of the Korean Society of Costume
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    • v.57 no.3 s.112
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    • pp.1-13
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    • 2007
  • The Purpose of this study is to analyze the kinds and positions of tattoo patterns on the body in tattoo-related internet communities and professional web sites. for this purpose, 1,892 tattoo patterns were analyzed by sex(man and woman). The results were as fellows; First, animal patterns(30.2%) occupied most, followed by character patterns(24.1%), geometric patterns(13.0%), natural patterns(10.3%), plant patterns(4.7%), mixed patterns(2.5%), and artificial patterns(2.2%). In patterns, dragon(10.3%) occupied most, followed by star(8.7%), trival(8.6%), woman(7.6%), skeleton(4.9%), and letter(4.8%). Second, men's preference to pattern groups included animal patterns(30.8%), character patterns (28.3%), geometric patterns (14.6%), and natural patterns(6.0%). Among patterns, dragon(13.4%) was the most frequent, followed by trival(10.9%), woman(10.7%), and skeleton(7.1%). Women's preference to patterns groups included animal patterns(31.4%), natural patterns(17.3%), character patterns(17.2%), geometric patterns(10.5%), and plant patterns(10.0%). Among patterns, star(15.3%) was the most frequent, followed by butter- fly(10.5%), elf(9.2%), and dragon(9.2%). Third, the positions of tattoos on the body included upper arm(26.6%), shoulder(10.8%), back(10.5%), the wrist(10.0%), the calf(7.5%), back bottom(7.0%) and the breast(6.3%). While men's preference to pattern positions included upper arm(38.2%), the wrist(13.7%), back(10.5%), the calf(9.4%), and shoulder(8.0%), women's preference to positions included back bottom(17.7%), shoulder(15.5%), back(10.5%), front bottom(8.2%), and the breast(7.8%).

Kinetics and Stereochemistry of CO Substitution Reactions of Half-Open Chromocene Carbonyls(Ⅲ): Reactions of $Cp^{*}(\eta^{5}-C_{5}H_{7})$CrCO and Phosphines

  • Jong-Jae Chung;Byung-Gill Roh;Yu-Chul Park
    • Bulletin of the Korean Chemical Society
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    • v.14 no.5
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    • pp.549-554
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    • 1993
  • The CO substitution reactions in the complex, $Cp^*(C_5H_7)$CrCO with $PR_3(PR_3=PMePh_2,\;P(OCH_3)_3,\;PMe_2Ph)$ were investigated spectrophotometrically at various temperatures. For the reaction rates, it was suggested that the CO substitution reaction took place by first-order (dissociative) pathway. Activation parameters in decaline are ${\Delta}H^{\neq}= 21.99{\pm}2.4$ kcal/mol, ${\Delta}S^{\neq}= 8.9{\pm}7.1$ cal/mol·k. Unusually low value of ${\Delta}S^{\neq}$, suggested an ${\eta}^5-S{\to}\;{\eta}^5$-U conversion of the pentadienyl ligand. At various temperature, the rates of reaction for the Cp(pdl)CrCO complexes increase in the order $Cp^*(C_5H_7)$-CrCO < Cp$(C_5H_7)$CrCO < Cp(2,4-$C_5H_{11}$)CrCO, which can be attributed to the usual steric acceration or electronic influence for the ligand substitution of metal complexes. This suggestion was confirmed by the extended-Huckel molecular orbital (EHMO) calculations, which revealed that the energy of $[Cp^*(U-C_5H_7)Cr]^{\neq}$ transition state is about 4.93 kcal/mol lower than that of [Cp(S-$C_5H_7)Cr]^{\neq}$ transition state, and the arrangement of the overlap populations between Cr and the carbon of CO is $Cp^*(C_5H_7)$CrCO > Cp($C_5H_7$)CrCO > Cp(2,4-$C_7H_{11}$)CrCO.

A Cross-sectional Survey on Drug Abuse among High School Students in Taegu City (대구시내 고등학생의 약물남용에 관한 연구)

  • Lee, Hyun-Sook;Kim, Chung-Nam;Oh, Yun-Jung
    • Research in Community and Public Health Nursing
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    • v.8 no.2
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    • pp.347-367
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    • 1997
  • The purpose of this study was to investigate drug abuse and to find related factors among high school students. The subjects for this study were 973 students from 10 high schools in Taegu city. The data were collected from September 30, 1996 to October 30, 1996. The questionnaire developed by Kim Soyoaja (1991) surveyed adolescent drug use and questions on smoking and drinking were readjusted and added by the researcher based on review. The data was analyzed using frequency, percentage, $X^2-test$, t -test, Pearson Correlation Coefficient with the SPSS /PC+. The results of this study were summarized as follows: 1. The proportion of students who drank alcohol 1-2 times or more per year was 52.4% and smoked Cigarette 1 -2 times or more per year was 20.8%. The 7 different drugs(Analgetics 5.7%. sleeping pills and sedatives 4.2%, antihistamines 1.1%. stimulants 7.7%, hallucinogens 0.8%, inhalants 3.3%, and narcotics 0.6%) were also evaluated. 2. There was a significant relationship between drinking and type of school($X^2$=62.97, p<.0l), grades($X^2$=33.86, P<.001), school life($X^2$= 19.04, p<.001), and delinquent friends($X^2$= 64.72, P<.001). 3. There was a significant relationship between smoking and type of school($X^2$=153.65, p<.001), grades ($X^2$=67.53, p<.001), their respect for teachers ($X^2$=33.80, p<.001) school life($X^2$ =50.87, p<.001), and delinquent friends($X^2$ =85.28, p<.001). 4. There was a significant relationship between the 7 different kinds of drug abuse and type of school ($X^2$=14.65, p<.01), grades($X^2$=12.89, p<.01), their respect for teachers ($X^2$=8.46, p<.05), and delinquent friends($X^2$=22.42, p<.001). 5. There was a significant relationship between a parent's habitual drug abuse and the 7 different kinds of drug abuse($X^2$=7.78, p<.01), as well as a parent's attitude toward drugs and the 7 different kinds of drug abuse($X^2$=6.33, p<.05). 6. There was a significant difference between drinking(t=-12.53, p<.001), smoking(t=-15.98, p<.001), the 7 different kinds of drug abuse(t=-5.77, p<.001), and the respondant's delinquent experience. 7. There was a correlation between drinking and smoking(r=.4166, p<.001), drinking and the 7 different kinds of drug abuse(r=.2200, p<.001), smoking and the 7 different kinds of drug abuse(r=.1428, p<.05). There was a correlation between drinking and smoking(r=.5977, p<.001), drinking and the 7 different kinds of drug abuse(r=.2849, p<. 001), smoking and the 7 different kinds of drug abuse(r=.1711, p<.05) among male students. There was a correlation between drinking and smoking(r=.4219, p<.001), drinking and the 7 different kinds of drug abuse(r=.2611, p<.001), smoking and the 7 different kinds of drug abuse(r=.1764, p<.001) among female students. 8. There was a correlation between drinking and family stability(r=.0709, p<.05) drinkry and parent -child relationships (r=.1321, p<.01), drinking and mother's rearing attitude(r=.0704, P<.05), smoking and parent -child relationships(r=.0813, P<.05). There was a correlation between drinking and family stability(r=.14S7, p<.01), drinkng and parent-child relationships(r=.2147, p<.001), smoking and family stability(r=.1544, p<.01), smoking and parent. -child relationships (r=. 2018, P<.01) among male students. There was a correlation between drinking and family stability(r=.1l21, p<.05), drinking and mother's rearing attitude (r=.0988, P<.05), smoking and parent -child relationships (r=. 0940, P<.05) among female students. 9. There was a significant difference between the 7 different kinds of drug abuse and family stability (t=2.23, p<.05), parent-child relationships (t=4. 34, p<.001), satisfaction with family (t=4.02, p<.001), father's rearing attitude(t=3.04, p<.01), mother's rearing attitude(t=2.87, p<.01). The distribution channel of drugs including alcohol beverages and cigarettes should be evaluated and restructured to discourage student's temptation and to limit accessibility. The step by step preventive teaching on alcohol drinking and cigarette smoking is needed from middle school to help prevent further drug abuse.

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The Kinematic Analysis of Handspring Salto Forward Piked (핸드스프링 몸접어 앞공중돌기동작의 운동학적 분석)

  • Kwon, Oh-Seok
    • Korean Journal of Applied Biomechanics
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    • v.17 no.1
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    • pp.145-153
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    • 2007
  • The purpose of this study is to compare and analyze the phase-by-phase elapsed time, the COG, the body joint angle changes and the angular velocities of each phase of Handspring Salto Forward Piked performed by 4 college gymnasts through 3D movement analysis program. 1. The average elapsed time for each phase was .13sec for Phase 1, .18sec for Phase 2, .4sec for Phase 3, and .3sec for Phase 5. The elapsed time for Phase 1 to Phase 3 handspring was .35sec on average and the elapsed time for Phase 4 to Phase 5 handspring salto forward piked was .7sec on average. And so it showed that the whole elapsed time was 1.44sec. 2. The average horizontal changes of COG were 93.2 cm at E1, 138. 5 cm at E2, 215.7 cm at E3, 369.2 cm at E4, 450.7 cm at E5, and 553.1 cm at E6. The average vertical changes of COG were 83.1 cm at E1, 71.3 cm at E2, 78.9 cm at E3, 93.7 cm at E4, 150.8 cm at E5, and 97.2 cm at E6. 3. The average shoulder joint angles at each phase were 131.6 deg at E1, 153.5 deg at E2, 135.4 deg at E3, 113.4 deg at E4, 39.6 deg at E5, and 67.5 deg at E6. And the average hip joint angles at each phase were 82.2 deg at E1, 60 deg at E2, 101.9 deg at E3, 161.2 deg at E4, 97.7 deg at E5, and 167 deg at E6. 4. The average shoulder joint angular velocities at each phase were 130.9deg/s E1, 73.1 deg/s at E2, -133.9 deg/s at E3, -194.4 deg/s at E4, 29.4 deg/s at E5, and -50.1 deg/s at E6. And the average hip joint angular velocities at each phase were -154.7 deg/s E1, -96.5 deg/s at E2, 495.9 deg/s at E3, 281.5 deg/s at E4, 90.3 deg/s at E5, and 181.7 deg/s at E6. The results shows that, as for the performance of handspring salto forward piked, it is important to move in short time and horizontally from the hop step to the point to place the hands on the floor and jump, and to stretch the hip joints as much as possible after the displacement of the hands and to keep the hip joints stretched and high in the vertical position at the takeoff. And it is also important to bend the shoulder joints and the hip joints fast and spin as much as possible after the takeoff, and to decrease the speed of spinning by bending he shoulder joints and the hip joints quickly after the highest point of COG and make a stable landing.

Isolation and Characterization of a Bacterium with a Fibrinolytic Activity (Fibrin 용해 균주의 분리 및 특성)

  • 정용준
    • KSBB Journal
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    • v.14 no.1
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    • pp.103-108
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    • 1999
  • A bacterium having strong fibrinolytic activity, S7-16 strain, was isolated from soil. The isolated bacterium was identified and named as Bacillus sp. S7-16. The optimal composition of the medium for the production of fibrinolytic enzyme by Bacillus sp. S7-16 was 0.5%(w/v) polypeptone, 0.5%(w/v) yeast extract, 0.3%(w/v) NaCl, 0.1% (w/v) $KH_2PO_4,\;0.3%(w/v)\;K_2PHO_4,\;and\;0.01%(w/v)\;MgSO_4{\cdot}7H_2O$. The optimal temperature and initial pH of the medium for the production of the enzyme were $35^{\circ}C$ and 7.0, respectively. The maximum production of the fibrinolytic enzyme was obtained after 24 hours of the incubation. Under the above conditions, the culture supernatant had strong fibrinolytic activity. Within pH4~11, the crude fibrinolytic enzyme was stable. The enzyme was stable up to $50^{\circ}C$. The optimum pH and temperature for the enzyme activity were around 7.5 and $40^{\circ}C$, respectively.

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Inhibition of Listeria monocytogenes by Low Concentrations of Ethanol (저농도의 Ethanol에 의한 Listeria monocytogenes의 증식억제)

  • 박찬성;김미림
    • Korean journal of food and cookery science
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    • v.11 no.4
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    • pp.379-385
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    • 1995
  • The effect of low concentrations of ethanol (3-7%, v/v) in tryptic soy broth (TSB) as an antibacterial agent against Listeria monocytogenes was tested at -20, 5, 35, 45, 50 and 55$^{\circ}C$. Increasing concentrations of ethanol progressively inhibited initial growth of L. monocytogenes at 35$^{\circ}C$. Growth occured at 5% ethanol, but only after a prolonged lag period. The number of viable cells of L. monocytogenes declined during incubation at 7% ethanol. TSB containing 3-7% ethanol was inoculated with 10$\^$5/-10$\^$6/ cells/$m\ell$ or L. monocytogenes and incubated at low temperatures (5$^{\circ}C$, -20$^{\circ}C$). In the presence of 3% of ethanol at 5 or -20$^{\circ}C$, bacterial growth was inhibited more than 90% of control cells. TSB containing 3-7% ethanol was inoculated with 10$\^$6/-10$\^$7/ cells/$m\ell$ of L. monocytogenes and incubated at high temperatures (45$^{\circ}C$, 50$^{\circ}C$, 55$^{\circ}C$). Decrease in viability of the cells incubated at 45 or 50$^{\circ}C$ was slow and the survival of L. monocytogenes was not affected so much in the presence of 3% of ethanol. The viability of L. monocytogenes was decreased with increasing concentration of ethanol and temperature. Decimal reduction times (D-values) based on tryptic soy agar plates at 55$^{\circ}C$ were 20.1, 12.6, 7.4 and 4.2 min in 0, 3, 5 and 7% ethanol, respectively.

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Types of Wedding Ceremony Robes in Jeju Area from the 1950's to the 1980's (1950-1980년대 제주 혼례복식의 유형분석)

  • Kim, Hyun-Mi
    • Journal of the Korean Society of Costume
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    • v.66 no.5
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    • pp.113-121
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    • 2016
  • The purpose of this study is to analyze the types and characteristics of wedding ceremony robes in the Jeju area from the 1950's to the 1980's. The analysis used 413 wedding pictures. The results of the study are as follows: 1. The following are the propotion of robe types used wedding ceremonies. 1.0% of Danryeong & Jangot, 6.5% of Danryeong & Wonsam, 1.5% of Danryeong & Chima+Jegori, 2.7% of Durumaki & Chima+Jegori, 24.7% of suit & Chima+Jegori and 63.7% of suit & wedding dress from the 1950's to the 1980's. 2. The Korean-style robes gradually decreased from the 1950's, and disappeared in the 1970's. The Chima+Jegori was introduced in the 1950's, reached its peak in the 1960's and its popularity continued to the 1970's. In the 1980's, after the Korean-style robes disappeared, the western ceremony robes were used in all the ceremonies. 3. After the Western-style robes, in which a bride wears a wedding dress, was introduced in the 1960's, its usage rapidly increased in the 1970's and is still popular today.

Nucleotide Sequence of Pre Protein in Chloramphenicol Resistance Plasmid pKH7. (클로람페니콜 내성 플라스미드 pKH7의 Pre 단백질의 염기서열 결정)

  • 문경호;박봉동;이동석;이백락
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.566-568
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    • 1998
  • Partial nucleotide sequence (nt 1-1842) of chloramphenicol resistance plasmid pKH7 has been reported previously and residual nucleotide sequence (nt 1843-4118) of pKH7 was determined and then the complete nucleotide sequence of pKH7 was obtained. pKH7 consists of 4118 bp and has three ORFs. Besides Rep and CAT proteins described in previous paper, Pre protein which mediates site-specific recombination in Staphylococcus aureus was found to be on pKH7. R $S_{A}$, a site-specific recombination site of Pre protein, and palA, a specific lagging-strand conversion signal, was also found in pKH7. Amino acid sequence of Pre protein of pKH7 was compared with those of other antibiotic resistant Staphylococcus aureus plasmids.s.

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Synthesis and Photoelectronic Properties of Thermally Stable Poly[oxy(2,7-fluoren-9-onenylene)oxy(diorganosilylene)]s

  • Jung, Eun-Ae;Park, Young-Tae
    • Bulletin of the Korean Chemical Society
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    • v.33 no.6
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    • pp.2031-2036
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    • 2012
  • Melt copolymerization reactions of several bis(diethylamino)silane derivatives, bis(diethylamino)methylphenylsilane, bis(diethylamino)methyloctylsilane, 1,2-bis(diethylamino)tetramethyldisilane, and 1,3-bis(diethylamino) tetramethyldisiloxane, with 2,7-dihydroxyfluoren-9-one were carried out to yield poly[oxy(2,7-fluoren- 9-onenylene)oxy(diorganosilylene)]s bearing the fluoren-9-one fluorescent aromatic group in the polymer main chain: poly[oxy(2,7-fluoren-9-onenylene)oxy(methylphenylsilylene)], poly[oxy(2,7-fluoren-9-onenylene) oxy(methyloctylsilylene)], poly[oxy(2,7-fluoren-9-onenylene)oxy(tetramethyldisilylene)], and poly[oxy- (2,7-fluoren-9-onenylene)oxy(tetramethyldisiloxanylene)]. These polymeric materials are soluble in common organic solvents such as $CHCl_3$ and THF. FTIR spectra of all the materials reveal characteristic Si-O-C stretching frequencies at 1012-1018 $cm^{-1}$. In the THF solution, the prepared materials show strong maximum absorption peaks at 258-270 nm, strong maximum excitation peaks at 260-280 nm, and strong maximum fluorescence emission bands at 310-420 nm. TGA thermograms suggest that most of the polymers are essentially stable to $200^{\circ}C$ without any weight loss and up to $300^{\circ}C$ with only a weight loss of less than 5% in nitrogen.