• 간호행정학회지
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• 제10권2호
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• pp.255-264
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• 2004

Purpose: The Purpose of this study was to identify the relationship between 7S factors of the nursing organizational culture and organizational effectiveness. Method: The data were gathered from the self-reported questionnaires of 717 nurses who work for eight different general hospitals located around Seoul and Kyounggi province. The period of data collection was from November 12 to December 7, 2002. For data analysis, descriptive statistics, clustering analysis, and t-test with SPSS Program were used. Result: The nurses who highly perceived 7S factors of nursing organizational culture showed higher job satisfaction and organizational commitment in comparison with the nurses who lowly perceived 7S factors of nursing organizational culture. And the nurses who highly perceived 7S factors of nursing organizational culture showed higher organizational citizenship behavior in comparison with the nurses who lowly perceived 7S factors of nursing organizational culture. Among subdimension of organizational citizenship behavior, altruism and civic virtue were significant. Conclusion: From the above results, the high group with 7S factors of nursing organizational culture has strong culture, therefore nursing organization with strong culture is very implicative to enhance the organizational effectiveness.

• 한국정보통신학회논문지
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• 제12권7호
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• pp.1301-1307
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• 2008

본 논문에서는 ARM 칩인 AT91SAM7S256, UHF RFID R/W 모듈인 WJ7090 및 무선 랜(IEEE 802.11.a/b)을 이용하여 UHF RFID R/W 전용 단말기를 개발하고 그 성능을 개선하였다. 또한 AT91SAM7S2S6에서 UHF R/W 모듈인 WJ7090으로 보내지는 송수신패킷을 개발하였다. 본 논문에서 개발한 무선랜(IEEE 802.11.a/b) 기반의 UHF RFID R/W 전용 단말기의 유용성을 평가하기 위해 성능 테스트를 실시하였다. 본 논문에서 개발한 UHF RFID R/W 전용 단말기는 RFID Tag값을 Tagging하고 그 값을 무선 랜(IEEE 802.11.a/b)을 통해서 서버로 전송하고, 그 결과를 단말기에 표시하였고, 성능실험을 통해 개발한 단말기의 우수성 및 송수신패킷에 대한 실험 결과를 나타내었다.

• 분석과학
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• 제18권3호
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• pp.224-231
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• 2005

잔류농약 분석에 전통적으로 사용되는 액체-액체 추출법과 액체-고체 추출법의 여러 가지 단점을 극복하고 기존의 낮은 정밀도와 저조한 추출효과를 보완하기위해 자동화된 장치를 이용한 액체상 극미량 추출법(Liquid Phase Microextraction : LPME)을 사용하였으며 이에 대한 최적화 조건을 설정하였다. 대상농약은 demeton-S-methyl, diazinon, parathion, fenitrothion, EPN등 5종이었으며 채소 시료 중에서 추출하여 HP 6890 GC/NPD를 사용하여 분석하였다. 자동화된 LPME를 이용한 최적의 추출조건은 pH 3, 염의 농도 $100{\mu}g/mL$이었으며 검량선을 작성하였을 때 $0.2{\sim}10{\mu}g/g$ 범위에서 $R^2=0.9921$ 이상 직선상의 상관관계를 나타내었고 $5{\mu}g/g$ 농도에서 demeton-S-methyl은 7.7%, diazinon은 9.8%, parathion은 7.8%, fenitrothion는 9.7%, EPN은 8.2%의 상대표준편차를 나타내었고 정확도는 demeton-S-methyl는 12.7%, diazinon는 7.8%, parathion는 10.4%, fenitrothion는 -6.7%, EPN은 -0.7%로 좋은 값을 나타내었다.

• 한국식품과학회지
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• 제20권3호
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• pp.344-349
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• 1988

분획(分劃)된 대두(大豆) 7S 및 11S 단백질(蛋白質)은 단백가수분해(蛋白加水分解) 효소(酵素)(alcalase 및 pronase)로 1시간(時間)동안 가수분해(加水分解)하였을 때 사용(使用)된 효소(酵素) 및 단백질(蛋白質) 종류(種類)에 관계없이 pH 5에서 용해도(溶解度), 열응고성(熱凝固性) 및 $Ca^{++}$에 대한 내침전성(耐沈殿性)은 상당히 증가(增加)에멀젼 활성 및 거품 안정성(安定性)은 감소(減少), 에멀젼 열안정성(熱安定性) 및 동점도(動粘度)는 거의 변화(變化)되지 않았다. 그러나 용해도(溶解度)에서 7S 단백질(蛋白質)은 pH 6에서 11S 단백질(蛋白質)은 pH 4에서 감소(減少)하였으며 또한 11S 단백질(蛋白質)은 유흡수성(油吸收性) 및 거품 형함능(形咸能)에서 가수분해(加水分解)에 의하여 증가(增加)하였으나 7S 단백질(蛋白質)은 거의 변화(變化)하지 않았다.

• 한국음향학회지
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• 제26권5호
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• pp.188-193
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• 2007

일반적으로 현재 사용하고 있는 홈시어터를 구성하기 위해서는 5.1 채널 이상의 아날로그 앰프를 주로 사용한다. 그러나 고성능의 시스템을 구성하기 위해서는 스피커의 출력이나 효율을 고려하여 음향 신호 처리를 디지털로 하는 것이 유리하다. 특히 음향을 효과적으로 분리하기 위해서는 7.1채널의 사용이 필요하다. 본 논문에서는 홈시어터 스피커를 위한 S/PDIF 7.1 채널 디지털 앰프의 구조를 연구하고 설계하였다. S/PDIF 7.1 채널 디지털 앰프는 노이즈에 강하고 S/PDIF를 사용하여 디지털 데이터를 직접 입력받아서 처리하기 때문에 디지털 원음의 손실이 적은 우수한 성능을 보여주고 있다.

• Food Science and Biotechnology
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• 제15권1호
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• pp.128-132
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• 2006

This study was undertaken to investigate the effect of peptic and chymotryptic hydrolyses of 7S globulin, the major allergen of soybean protein, on its allergenicity, as measured by enzyme linked immunosorbent assay (ELISA), and to identify the allergenic hydrolyzed fragments of 7S globulin using SDS-PAGE. When 7S globulin was hydrolyzed by pepsin, the allergenicity was reduced by over 50%. However, the allergenicity of 7S globulin reduced by peptic hydrolysis was recovered in the sera from 5 out of 10 patients following sequential chymotryptic hydrolysis. Two fragments, with molecular weights 20-25 and 13-16 kDa, among the hydrolysate of 7S globulin by sequential pepsin and chymotrypsin showed reactivity with sera from 10 soybean-allergenic patients. As a result of the theoretical hydrolyses of ${\beta}$-conglycinin, which is a major protein of 7S globulin, it is suggested that the 20-25 kDa fragments were the fragments of the ${\alpha}$-subunit of ${\beta}$'-conglycinin and that the 10-16 kDa fragments were from the ${\alpha}$'-subunit.

• 대한본초학회지
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• 제28권2호
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• pp.83-92
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• 2013

Objectives : We investigated differances of physiological functionalities in the steamed Schisandrae Fructus. Methods : The samples were extracts of dryed schisandrae fructus without steaming process (S0), extracts of schisandrae fructus with three times steamed (S3), extracts of schisandrae fructus with five times steamed (S5) and extracts of schisandrae fructus with seven times steamed (S7). We analyzed contents of schisandrin, gomisin, total polyphenol and flavonoid and antioxidant activities. We researched antiinflammation effects for Raw264.7 cells. To evaluate liver protective activity, we measured AST, ALT and gamma-GTP in serum of alcoholic mice. Results : As the steaming number of schisandrae fructus increase, the contents of schisandrin and gomisin were more increased. The contents of total polyphenol of S5 and S7 were significantly increased compared to that of S0. DPPH free radical scavenging activities of S5 and S7 were significantly increased compared to that of S0, ABST radical scavenging activities of S3 and S5 were significantly increased compared to that of S0 in vitro. The NO production of all sample was significantly decreased compared to control, PGE2 release of S3, S5 and S7 were significantly decreased compared to control. IL-$1{\beta}$ release of S5 and S7 were significantly decreased. AST, ALT and gamma-GTP of S3, S5 and S7 were significantly decreased compared to control. Conclusions : We think that extracts of schisandrae fructus with steaming process may have more potential efficacy than a schisandrae fructus without steaming process.

• Journal of Applied Biological Chemistry
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• 제62권1호
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• pp.73-79
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• 2019

A novel esterase gene (est7S) was cloned from an enriched metagenomic library derived from an oil-spill area. The gene encoded a protein of 505 amino acids, and the molecular mass of the Est7S was estimated to be 54,512 Da with no signal peptide. Est7S showed the highest identity of 40% to an esterase from a sludge metagenome compared to the characterized enzymes with their properties, although it showed 99% identity to a carboxylesterase in the genome sequence of Alcanivorax borkumensis SK2. Est7S had catalytic triad residues, Ser183, Glu312, and His420, and the GESAG motif in most family VII lipolytic enzymes. Est7S was purified from the crude extract of clone SM7 using Sephacryl S-200 HR and HiTrap Q column chromatographies. The purified Est7S was optimally active at $50^{\circ}C$ and pH 10.0. Est7S showed a high specific activity of 366.7 U/mg protein. It preferred short length esters, particularly p-nitrophenyl acetate, efficiently hydrolyzed R- and S-enantiomers of methyl-3-hydroxy-2-methylpropionate, and glyceryl tributyrate. These properties of Est7S may provide potential merits in biotechnological applications such as detergent and paper processing under alkaline conditions.

• 한국식품위생안전성학회지
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• 제30권2호
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• pp.210-216
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• 2015

본 연구는 마늘의 물추출물(AGE)의 E. coli O157:H7, S. typhimurium, 그리고 S. aureus에 대한 항균효과를 조사하였다. AGE의 E. coli O157:H7, S. typhimurium, and S. aureus에 대한 최소억제농도(MIC)는 각각 24, 48 그리고 24 mg/mL이었으며, 최소살균농도(MBC)는 모든 균에 대하여 96 mg/mL이었다. AGE 24 mg/mL을 E. coli O157:H7에 투여하고 배양 24시간 후에, 균의 증식이 무처리 대조군과 비교하여 유의성 있게 감소하였다(p < 0.01). 그러나 AGE 24 mg/mL을 S. aureus에 투여하고 배양 24시간 후에, 대조군과 비교하여 유의성 있는 균의 증식억제 효과가 나타나지 않았으나, AGE 96 mg/mL을 투여한 경우에서는 배양 24시간 후에 대조군과 비교하여 유의성 있는 균의 증식억제가 나타났다(p < 0.01). AGE 48 (p < 0.05)과 96 mg/mL (p < 0.001)을 S. typhimurim에 투여하고 24시간 배양 후에, 대조군과 비교하여 통계적으로 유의성 있는 균의 증식억제 효과가 나타났다. 본 연구의 결과로부터, 마늘의 물추출물은 항생제와 화학적 식품보존제의 대체제로서 사용이 가능할 것으로 사료된다.

• 한국축산식품학회지
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• 제18권1호
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• pp.63-74
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• 1998

Recently the high outbreaks of intestinal disease caused by the consumption of frozen dairy foods containing pathogenic bacteria has generated considerable interest in the causative agent such as Listeria monocytogenes and E. coli O157:H7. This study was carried out to detect the above pathogens and compare the microbiological qualities of three commercial forzen yogurt products. The main results obtained were as follows. L. monocytogenes coliforms and E. coli O157:H7 were not detected in the total of seven frozen yogurt samples. For microbiological qualities the viable lactic counts of products manufactured by FA company were about 2.9$\times$108 -1.6$\times$109cfu/ml 1.7$\times$106 cfu/ml for FB's and 1.2$\times$106 cfu/ml for FC's The PH values of FA's FB's and FC's products was in the range of pH 4.1~5.3 and the values of FA's were 4.1~4.6 compared by the pH 5.2~5.3 of FB's and FC's products. During refrigeration of the test samples the survival rates of L. monocytogenes spiked into thawed frozen yogurt sample(FA's FB,s and FC's) were 0.55% 15.61% and 16.89% respectively. On the other hand E. coli O157:H7 and L. monocytogenes were 12.4% and 25.0% for FA's 10.8% and 20.8% for FB's and 10.26% and 22.7% for FC's under 37$^{\circ}C$ storage, As the results described above each frozen yogurt products were different in microbiological qualities. The survival rates of pathogens spiked into the samples increased with the pH of the products. This indicates that the pH or any other factors pre-sumable supressed the growth of E. coli O157:H7 and L. monocytogenes in frozen yogurt products.