• Journal of Animal Reproduction and Biotechnology
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• v.34 no.4
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• pp.280-291
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• 2019

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.

• Journal of Korea Water Resources Association
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• v.49 no.7
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• pp.615-623
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• 2016

This Study calculated the Submerged Flow Characteristics and Discharge coefficient by the rising angular change of the Improved-Pneumatic-Movable. According to the result, the smaller the ratio of weir height and weir length (L/W) or the weir standing angle, the bigger of the downstream head ($H_2$). The change of discharge reduction factor ($Q_s/Q_1$), by the hight from weir crest to downstream surface and the ratio form weir crest to upstream water height ($h_t/H$), was decreased when the $h_t/H$ closed to number 1. Although the weir water depth of the down-stream was shallower level than the up-stream, the velocity was faster then before. And the more the flow, the less the gab between the upper and lower reaches level. And when the same flow condition, the downstream head ($H_2$) was increased when the L/W was bigger. The Submerged Flow Discharge coefficient of Improved-Pneumatic-Movable weir was made by the upstream approach flow head and the upper lower stream flow condition, not by the physical data of Movable weir.

• Mass Spectrometry Letters
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• v.11 no.2
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• pp.36-40
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• 2020

Untargeted metabolomics is a useful tool for drug development focusing on novel chemotherapeutic and chemopreventative agents against cancer cells. In recent years, quadrupole time of flight liquid chromatography-mass spectrometry (Q-TOF LC/MS)-based untargeted metabolomic approaches have gained importance to evaluate the effect of these agents at the molecular level. The researchers working on cell culture studies still do not apply standardized methodologies on sample preparation for untargeted metabolomics approaches. In this study, the rough and wet lysis techniques performed on MCF-7 breast cancer cells were compared with each other via the Q-TOF LC/MS-based metabolomic approach. The C18 and hydrophilic interaction liquid chromatography (HILIC) columns were used for the separation of the metabolites in MCF-7 cell lysates. 505 peaks were detected through the HILIC column and 551 peaks were found through the C18 column for the wet lysis technique. This situation supported by the base peak chromatograms showed that the wet lysis technique allowed us to extract higher number of non-polar metabolites. Almost equal number of metabolites was found for the C18 and HILIC columns (697 peaks for the HILIC column and 695 peaks for the C18 column) when the rough lysis technique was used. However, the intensities of polar metabolites were higher for the rough lysis technique on base peak chromatograms for both the HILIC and C18 columns. Although cell lysis technique, which is the first step in the sample preparation for cell culture studies, does not cause dramatic differences in the number of the detected metabolite peaks, it affects the polar and non-polar metabolite ratio significantly. Therefore, it must be considered carefully especially for in vitro drug development studies.

• Journal of Life Science
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• v.18 no.1
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• pp.30-37
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• 2008

The purpose of this study was to investigate the effect of Isotonic Training and Isotonic isometric complex Training to Knee joint Isokinetic muscle function in the high-school Taekwondo athlete. This subjects were randomly divided into two groups; a Isotonic Training group (n=7) Isotonic isometric complex Training group (n=7), Taekwondo athletes share each, 12 weeks between executes a motion. peak torque, H/Q ratio, bilateral muscular ratio muscular endurance of knee joint measured. For this study, two-way ANOVA with repeated measure were used. The result of this study presents between groups was not plain difference expressed. H/Q ratio, bilateral muscular ratio, muscular endurance was plain difference expressed after 12 weeks point of time (p>0.05) and peak torque was plain difference expressed (p<0.05). Conclusion each group did not appear all but one (peak torque), but point of view exercise was effect H/Q ratio, bilateral muscular ratio, muscular endurance, peak torque plain difference expressed.

• Journal of Korea Game Society
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• v.15 no.4
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• pp.7-18
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• 2015

A social network game (SNG) is the result of a combination of a life simulation game and a social networking service. These types of games are particularly popular among women. This phenomenon calls for research that focuses on the motivation behind playing SNGs, the factors that make SNGs absorbing and, particularly, the space decorating features of SNGs. The current study investigates people's experience with the space decorating features of SNGs and their motivation for playing these games. It observes the behaviors of SNG players, which were typed through Q methodology. Moreover, it specifically analyzes the motivation behind the playing of space-decorating SNGs. The study concludes that most of players were more absorbed in the space-decorating features of these games than they were in their social networking features. This also affects a player's continued playing of these games. This study proves that this phenomenon is related to players' desires for self-representation.

• Journal of Digital Convergence
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• v.14 no.7
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• pp.355-362
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• 2016

This research analyzed 'esquisse', that is one of among the visual representation tools of designers in design-thinking process. They were classified into five different types(M, N, O, P, Q) and categorized about preferred type of brain dominance area based on Ned Herrmann's 'Brain 4 division theory'. By contrasting and analyzing five types of sketch tendencies and brain tendencies through Structural left -brain type(M), Emotional limbic-brain type(N), Visual right-brain type(O), Plane expressional right-brain type(P) and Text expressional right-brain type(Q), it was deduced that which utilization the designer with each brain type with various Styles and characteristics shows internally in the design thinking process can be analyzed.

• Journal of Construction Engineering and Project Management
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• v.1 no.3
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• pp.1-8
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• 2011

Available data on the recent global financial crisis (GFC) show that it lasted between the second quarter (Q2) of 2007 and the fourth quarter (Q4) of 2009. Australia is one of the first economies to fully recover from this crisis. This study explorles the role played by the Australian construction industry in stimulating economic growth during the recession. In order to investigate the macro-variability trend during the financial crisis, data were collected and analysed relating to the quarterly GDP of Australia and selected countries between Q1 2000 and Q4 2009. Specifically, changes in the construction industry's GDP were compared with aggregate GDP changes in Australian economy and similar indices in the 'Group of 7' (G7) countries and Organisation for Economic Co-operation and Development (OECD) countries. Moreover, specific attention was focused on Germany, France, Japan, United States of America (USA) and United Kingdom (UK). Graphical and Pearson's correlation methods were used to analyse the relationships between changes in construction GDP and Australia's overall economic growth during the recession. In addition, an attempt was made to develop a regression model for predicting economic growth during the recent recession using changes in gross fixed capital formation (GFCF), changes in construction GDP and the impact of these changes on national economy. Analysis shows a slight contraction in construction activities during the crisis; however construction triggered significant growth in the economy during the crisis period and afterwards. This appears to be the major difference between Australia and other major economies that have experienced a longer recession.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.1 s.116
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• pp.15-23
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• 2007

A simple six-port direct conversion QPSK receiver which is made up of a six-port phase correlator, a signal power detector, and I/Q channel signal de-modulator is designed and implemented in this paper. The output phase signals of six-port phase correlator are also analysed. On the basis of $90^{\circ}C$ phase relation among the six-port phase correlator output signals, the QPSK de-modulation circuit is designed by a simple circuit. The six-port phase correlator is made up of $90^{\circ}$ hybrid branch line and power detector. The six-port phase correlator, which is designed in frequency range of 11.7 to 12.0 GHz, gets the phase error characteristics less than $5^{\circ}$. By considering matching network and amplitude balance in the designed fiequency range, the designed six-port direct conversion QPSK receiver demodulates the I and Q signals with performance less than $5^{\circ}$ phase error.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.26 no.5
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• pp.1089-1097
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• 2016

It is well-known that, if additional information other than a plaintext-ciphertext pair is available, breaking the RSA cryptosystem may be much easier than factorizing the RSA modulus. For example, Coppersmith showed that, given the 1/2 fraction of the least or most significant bits of one of two RSA primes, the RSA modulus can be factorized in a polynomial time. More recently, Henecka et. al showed that the RSA private key of the form (p, q, d, $d_p$, $d_q$) can efficiently be recovered whenever the bits of the private key are erroneous with error rate less than 23.7%. It is notable that their algorithm is based on counting the matching bits between the candidate key bit string and the given decayed RSA private key bit string. And, extending the algorithm, this paper proposes a new RSA private key recovery algorithm using a generalized probabilistic measure for measuring the consistency between the candidate key bits and the given decayed RSA private key bits.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.16 no.6
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• pp.95-109
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• 2006

Elliptic Curve Cryptosystem (ECC) is suitable for resource-constrained devices such as smartcards, and sensor motes because of its short key size. This paper presents an efficient multi-scalar multiplication algorithm which is the main component of the verification procedure in Elliptic Curve Digital Signature Algorithm (ECDSA). The proposed algorithm can make use of a precomputed table of variable size and provides an optimal efficiency for that precomputed table. Furthermore, the given scalar is receded on-the-fly so that it can be merged with the main multiplication procedure. This can achieve more savings on memory than other receding algorithms. Through experiments, we have found that the optimal sizes of precomputed tables are 7 and 15 when uP+vQ is computed for u, v of 163 bits and 233 bits integers. This is shown by comparing the computation time taken by the proposed algorithm and other existing algorithms.